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The map label for this gene is amiE [H]

Identifier: 218531612

GI number: 218531612

Start: 3878913

End: 3879965

Strand: Reverse

Name: amiE [H]

Synonym: Mchl_3681

Alternate gene names: 218531612

Gene position: 3879965-3878913 (Counterclockwise)

Preceding gene: 218531613

Following gene: 218531610

Centisome position: 67.15

GC content: 63.72

Gene sequence:

>1053_bases
ATGATGCACGGTGATATCTCGTCGAGTCAGGACAGCGTCGGCGTCGCCGTCGTGAACTACAAGATGCCCCGGCTGCACAC
CAAGGCCGAGGTGTTGGAAAACGCCAAGAAGATCGGCGACATGATCATCGGGATGAAGGCCGGTCTGCCGGGCCTCGATC
TCGTGATCTTCCCCGAATACTCGACCCACGGCATCATGTACGACGCGGGCGAGATGTACGAGACCGCCTCCTCCATCCCC
GGTGCCGAGACCGACATCTTCGCCGACGCCTGCCGCAAGGCCAAGGTCTGGGGCGTGTTCTCACTCACCGGCGAGCGTCA
CGAGGAGCATCCGCGCAAAGCCCCCTACAACACGCTCATCCTGATGAACGACCAGGGTGAGATCGTGCAGAAGTACCGCA
AGATCATGCCCTGGACCCCGATCGAGGGCTGGTATCCGGGCGACCGCACCTACGTCTCGGATGGACCGAAGGGCCTCAAG
ATCAGCCTGATCATCTGCGACGACGGCAACTATCCCGAGATCTGGCGCGACTGCGCGATGCGCGGCGCCGAACTCATCAT
CCGCTGCCAGGGCTACATGTACCCGGCCAAGGAACAACAGGTTCTGATCTCGAAGGCGATGGCATTCGCCAACAACACCT
ACGTCGCGGTGGCCAACGCCGCCGGCTTCGACGGCGTCTACAGCTATTTCGGCCACTCGGCGATCATCGGCTTCGACGGT
CGGACGCTGGGCGAGTGCGGCGAGGAGGAGATGGGCATCCAGTACGCCGCACTCTCGAAGTTCCTGATCCGCGATTTCCG
CGCGCACGGCCAGTCGGAGAACCACCTCTTCAAGCTGCTGCACCGCGGCTACACCGGCATGATCAACTCGAAGGAGAACC
GGCACGGGATGTCGGAATGCCCCTACGACTTCTACCGCCGCTGGATCGAAGACCCCGACGGCACCCGCGACGCGGTGCGC
GCCATCACCCGCTCCAGCGTCGGCACCGAGGAATGCCCGATCGAGGGCATCCCCTTCGTCGGCAAGGGCGAAGGCCCGCC
CGACCCGCGCTGA

Upstream 100 bases:

>100_bases
CCCTGCTCGGCCTCGCTCCGGGCTAGCCCTTTTTCCGAGCCAAGAAAGGGGGCTCGCGCGACGGGCCCCTCCCCTCACAC
CAACCGGAGGAAACACCGCC

Downstream 100 bases:

>100_bases
GCTCGGCTCGGGCCGAAACGCCACGTCATGCGGCCGAGCGCCCTCCACCCGAACCGGGTGGAGGGCGTTTTCCGTTGTGC
GCATGCGGGACGTTTGTCGG

Product: acylamide amidohydrolase

Products: NA

Alternate protein names: Acylamide amidohydrolase [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MMHGDISSSQDSVGVAVVNYKMPRLHTKAEVLENAKKIGDMIIGMKAGLPGLDLVIFPEYSTHGIMYDAGEMYETASSIP
GAETDIFADACRKAKVWGVFSLTGERHEEHPRKAPYNTLILMNDQGEIVQKYRKIMPWTPIEGWYPGDRTYVSDGPKGLK
ISLIICDDGNYPEIWRDCAMRGAELIIRCQGYMYPAKEQQVLISKAMAFANNTYVAVANAAGFDGVYSYFGHSAIIGFDG
RTLGECGEEEMGIQYAALSKFLIRDFRAHGQSENHLFKLLHRGYTGMINSKENRHGMSECPYDFYRRWIEDPDGTRDAVR
AITRSSVGTEECPIEGIPFVGKGEGPPDPR

Sequences:

>Translated_350_residues
MMHGDISSSQDSVGVAVVNYKMPRLHTKAEVLENAKKIGDMIIGMKAGLPGLDLVIFPEYSTHGIMYDAGEMYETASSIP
GAETDIFADACRKAKVWGVFSLTGERHEEHPRKAPYNTLILMNDQGEIVQKYRKIMPWTPIEGWYPGDRTYVSDGPKGLK
ISLIICDDGNYPEIWRDCAMRGAELIIRCQGYMYPAKEQQVLISKAMAFANNTYVAVANAAGFDGVYSYFGHSAIIGFDG
RTLGECGEEEMGIQYAALSKFLIRDFRAHGQSENHLFKLLHRGYTGMINSKENRHGMSECPYDFYRRWIEDPDGTRDAVR
AITRSSVGTEECPIEGIPFVGKGEGPPDPR
>Mature_350_residues
MMHGDISSSQDSVGVAVVNYKMPRLHTKAEVLENAKKIGDMIIGMKAGLPGLDLVIFPEYSTHGIMYDAGEMYETASSIP
GAETDIFADACRKAKVWGVFSLTGERHEEHPRKAPYNTLILMNDQGEIVQKYRKIMPWTPIEGWYPGDRTYVSDGPKGLK
ISLIICDDGNYPEIWRDCAMRGAELIIRCQGYMYPAKEQQVLISKAMAFANNTYVAVANAAGFDGVYSYFGHSAIIGFDG
RTLGECGEEEMGIQYAALSKFLIRDFRAHGQSENHLFKLLHRGYTGMINSKENRHGMSECPYDFYRRWIEDPDGTRDAVR
AITRSSVGTEECPIEGIPFVGKGEGPPDPR

Specific function: Also exhibits in vitro acyl transferase activity, transferring the acyl moiety of short-chain amides to hydroxylamine to form hydroxamates [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 39035; Mature: 39035

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHGDISSSQDSVGVAVVNYKMPRLHTKAEVLENAKKIGDMIIGMKAGLPGLDLVIFPEY
CCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHEEEHCCCCCCCEEEEECCC
STHGIMYDAGEMYETASSIPGAETDIFADACRKAKVWGVFSLTGERHEEHPRKAPYNTLI
CCCCEEECCHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEEEECCCHHHCCCCCCCCCEEE
LMNDQGEIVQKYRKIMPWTPIEGWYPGDRTYVSDGPKGLKISLIICDDGNYPEIWRDCAM
EECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHH
RGAELIIRCQGYMYPAKEQQVLISKAMAFANNTYVAVANAAGFDGVYSYFGHSAIIGFDG
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCEEEEECCC
RTLGECGEEEMGIQYAALSKFLIRDFRAHGQSENHLFKLLHRGYTGMINSKENRHGMSEC
CCHHHCCCHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PYDFYRRWIEDPDGTRDAVRAITRSSVGTEECPIEGIPFVGKGEGPPDPR
CHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMHGDISSSQDSVGVAVVNYKMPRLHTKAEVLENAKKIGDMIIGMKAGLPGLDLVIFPEY
CCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHEEEHCCCCCCCEEEEECCC
STHGIMYDAGEMYETASSIPGAETDIFADACRKAKVWGVFSLTGERHEEHPRKAPYNTLI
CCCCEEECCHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEEEECCCHHHCCCCCCCCCEEE
LMNDQGEIVQKYRKIMPWTPIEGWYPGDRTYVSDGPKGLKISLIICDDGNYPEIWRDCAM
EECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHH
RGAELIIRCQGYMYPAKEQQVLISKAMAFANNTYVAVANAAGFDGVYSYFGHSAIIGFDG
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCEEEEECCC
RTLGECGEEEMGIQYAALSKFLIRDFRAHGQSENHLFKLLHRGYTGMINSKENRHGMSEC
CCHHHCCCHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PYDFYRRWIEDPDGTRDAVRAITRSSVGTEECPIEGIPFVGKGEGPPDPR
CHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]