Click here to switch to the map view.

The map label for this gene is frdA [H]

Identifier: 218531607

GI number: 218531607

Start: 3871376

End: 3873124

Strand: Reverse

Name: frdA [H]

Synonym: Mchl_3676

Alternate gene names: 218531607

Gene position: 3873124-3871376 (Counterclockwise)

Preceding gene: 218531608

Following gene: 218531606

Centisome position: 67.03

GC content: 66.5

Gene sequence:

>1749_bases
ATGAGCGCAGCGCAAGACGCGATTCAGGACGCGACCTCGGAGGTCGCCTGCGACGTGCTGGTGATCGGCGGCGGCACCGC
CGGGCCGATGGCGGCGCTGAAGGCCAAGCTGCGCAACCCGGCAGCGCGGGTGATCCTGTTGGAGAAGGCCAACGTGAAGC
GCTCGGGCGCGATCAGCATGGGCATGGACGGGCTCAACAACGCCGTCATCCCCGGCTACGCCACGCCGGAGCAGTACACC
AAGGAAATCACCATCGCGAACGACGGCATCTGCGATCAGGCGCCGGTCTACAAGTATGCCGAAAACTGCTTCGAGATCAT
CCAGGAACTCGACCGCTTCGGCATCCGCTTCCAGCGCGACGGCAACGGCGATTACGACGTCAAGAAGGTGCACCATCTCG
GCACCTACGTGCTGCCGATGCCCAACGGCGACACCGTCAAGAAGGCGCTCTACCGGCAATTGCGCAAGGCGCAGGTGCTG
ATCTCGAACCGCTTCATGGCGACCCGGCTTCTCAACGCGCCCGACGGCGCGGTGGCCGGGGCGGTGGCGGTCAACACCCG
CACCGCCGAGTTCCTGGTGATCCGCGCCAAGGCGGTGATCCTCTGCCTCGGGGCGGCCGGGCGGCTCGGCCTGCCGCATT
CGGGCTATCTGTGGGGCACCTACGAGAACGCCGCCAATTCCGGCGACGGCTACTCGATGGCCTACCACGCGGGTGCCGGG
CTCGCGAACCTCGAATGCTTTCAGATCAACCCGCTGATCAAGGACTATAACGGCCCCGCCTGCGCCTACGTCGCCGGGCC
GTTCGGCGCCTACACCGCCAACAGCGAGGGCAACCGCTTCATCGAGTGCGACTACTGGTCGGGCCAGATGATGCAGGAAT
TCTACAACGAACTCTCGAGCGGCAAAGGCCCGGTCTTCCTCAAGCTCGACCATCTCCACGCCGACACGGTCTCGGAGATC
GAGACGATCCTGCACAAGGTCGAGCGGCCCTCGCGCGGACGCTTCCACGAGAACCGCGGCACCGATTACCGGAGCCGTAT
GGTCGAGATGCACATCTCGGAGGTGGGTTTCTGCTCCGGCCACAGCGCATCGGGCGTGTTCGTGGATGAATTCGCCCGCA
CCACCGTGCCGGGCCTGTATTCGGCCGGCGACATGGCCAACGTCCCCCACAACTACATGCTCGGCGCCTTCACCAACGGG
GCGGTGGCGGGTGAGCACGCGATCGATCATGCGGGCGCGATCGACCTGCCTGAGATCGATGCCGAGGCGGTGGCGCGCGA
GCGCGAGCGGGTGCTGGCGCCGACGCGGCGCGCGGACGGCATTCCCCCGAACCAGATCGAGTACAAGACCCGCCGCTTCG
TCAACGACTACCTGCAGCCGCCCAAGGTCACGGCGAAGATGGAGATCGGGCAGTGGCGCTTTGCCGAGATCCGCGAGGAT
CTGGAGACGCGGATGGTCGCCCGCGACAGCCACGAACTGATGCGGGCGCTGGAGGCGTCCTCGATCCTCGACTGCGCCGA
GATGGCCGCCCACGCCTCGCTCTACCGCACCGAGAGCCGCTGGGGCCTCTACCACAACCGCACAGACTTCCCCGAGAAGA
ACGACGCGGAGTGGTTCTGCCACACGATCCTGCGGAAGGTGGAGGGCCGCATGGTCAGCGAGAAGCGGCCGATCACGCCG
TACATCGTGCCGATCGAGGCGGACGAGCGCGGCGCCTACGACCGCCAGCGTGTCCGCAAGCAGGCTTGA

Upstream 100 bases:

>100_bases
GGCACGACCGCGGGATCTTCCCCTGGTCCTACCTGCGCGCGCTCGGCGACGACATGAGCCCGTGCGCGGCCGAACGCGCG
GCAGCGTGAGGGAGACGGCG

Downstream 100 bases:

>100_bases
GCCCGGAGACATCCATGCCCCTCGCCCACCAGCCCAGCGCGGTCCCGGTCACCGTGGACCCGGAAAAATGCATCGCCGAC
AAAGGCTGCACGGTCTGCGT

Product: oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 581

Protein sequence:

>582_residues
MSAAQDAIQDATSEVACDVLVIGGGTAGPMAALKAKLRNPAARVILLEKANVKRSGAISMGMDGLNNAVIPGYATPEQYT
KEITIANDGICDQAPVYKYAENCFEIIQELDRFGIRFQRDGNGDYDVKKVHHLGTYVLPMPNGDTVKKALYRQLRKAQVL
ISNRFMATRLLNAPDGAVAGAVAVNTRTAEFLVIRAKAVILCLGAAGRLGLPHSGYLWGTYENAANSGDGYSMAYHAGAG
LANLECFQINPLIKDYNGPACAYVAGPFGAYTANSEGNRFIECDYWSGQMMQEFYNELSSGKGPVFLKLDHLHADTVSEI
ETILHKVERPSRGRFHENRGTDYRSRMVEMHISEVGFCSGHSASGVFVDEFARTTVPGLYSAGDMANVPHNYMLGAFTNG
AVAGEHAIDHAGAIDLPEIDAEAVARERERVLAPTRRADGIPPNQIEYKTRRFVNDYLQPPKVTAKMEIGQWRFAEIRED
LETRMVARDSHELMRALEASSILDCAEMAAHASLYRTESRWGLYHNRTDFPEKNDAEWFCHTILRKVEGRMVSEKRPITP
YIVPIEADERGAYDRQRVRKQA

Sequences:

>Translated_582_residues
MSAAQDAIQDATSEVACDVLVIGGGTAGPMAALKAKLRNPAARVILLEKANVKRSGAISMGMDGLNNAVIPGYATPEQYT
KEITIANDGICDQAPVYKYAENCFEIIQELDRFGIRFQRDGNGDYDVKKVHHLGTYVLPMPNGDTVKKALYRQLRKAQVL
ISNRFMATRLLNAPDGAVAGAVAVNTRTAEFLVIRAKAVILCLGAAGRLGLPHSGYLWGTYENAANSGDGYSMAYHAGAG
LANLECFQINPLIKDYNGPACAYVAGPFGAYTANSEGNRFIECDYWSGQMMQEFYNELSSGKGPVFLKLDHLHADTVSEI
ETILHKVERPSRGRFHENRGTDYRSRMVEMHISEVGFCSGHSASGVFVDEFARTTVPGLYSAGDMANVPHNYMLGAFTNG
AVAGEHAIDHAGAIDLPEIDAEAVARERERVLAPTRRADGIPPNQIEYKTRRFVNDYLQPPKVTAKMEIGQWRFAEIRED
LETRMVARDSHELMRALEASSILDCAEMAAHASLYRTESRWGLYHNRTDFPEKNDAEWFCHTILRKVEGRMVSEKRPITP
YIVPIEADERGAYDRQRVRKQA
>Mature_581_residues
SAAQDAIQDATSEVACDVLVIGGGTAGPMAALKAKLRNPAARVILLEKANVKRSGAISMGMDGLNNAVIPGYATPEQYTK
EITIANDGICDQAPVYKYAENCFEIIQELDRFGIRFQRDGNGDYDVKKVHHLGTYVLPMPNGDTVKKALYRQLRKAQVLI
SNRFMATRLLNAPDGAVAGAVAVNTRTAEFLVIRAKAVILCLGAAGRLGLPHSGYLWGTYENAANSGDGYSMAYHAGAGL
ANLECFQINPLIKDYNGPACAYVAGPFGAYTANSEGNRFIECDYWSGQMMQEFYNELSSGKGPVFLKLDHLHADTVSEIE
TILHKVERPSRGRFHENRGTDYRSRMVEMHISEVGFCSGHSASGVFVDEFARTTVPGLYSAGDMANVPHNYMLGAFTNGA
VAGEHAIDHAGAIDLPEIDAEAVARERERVLAPTRRADGIPPNQIEYKTRRFVNDYLQPPKVTAKMEIGQWRFAEIREDL
ETRMVARDSHELMRALEASSILDCAEMAAHASLYRTESRWGLYHNRTDFPEKNDAEWFCHTILRKVEGRMVSEKRPITPY
IVPIEADERGAYDRQRVRKQA

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786942, Length=530, Percent_Identity=24.7169811320755, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1790597, Length=597, Percent_Identity=23.1155778894472, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR003952
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005884
- InterPro:   IPR014006 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.3.99.1 [H]

Molecular weight: Translated: 64340; Mature: 64208

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAQDAIQDATSEVACDVLVIGGGTAGPMAALKAKLRNPAARVILLEKANVKRSGAISM
CCHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEE
GMDGLNNAVIPGYATPEQYTKEITIANDGICDQAPVYKYAENCFEIIQELDRFGIRFQRD
CCCCCCCCCCCCCCCHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEC
GNGDYDVKKVHHLGTYVLPMPNGDTVKKALYRQLRKAQVLISNRFMATRLLNAPDGAVAG
CCCCCHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEE
AVAVNTRTAEFLVIRAKAVILCLGAAGRLGLPHSGYLWGTYENAANSGDGYSMAYHAGAG
EEEECCCCEEEEEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCC
LANLECFQINPLIKDYNGPACAYVAGPFGAYTANSEGNRFIECDYWSGQMMQEFYNELSS
CCCEEEEEECCHHHCCCCCEEEEEECCCCCEECCCCCCEEEEEECCCCHHHHHHHHHHHC
GKGPVFLKLDHLHADTVSEIETILHKVERPSRGRFHENRGTDYRSRMVEMHISEVGFCSG
CCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
HSASGVFVDEFARTTVPGLYSAGDMANVPHNYMLGAFTNGAVAGEHAIDHAGAIDLPEID
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHCCCCCCCCCCCC
AEAVARERERVLAPTRRADGIPPNQIEYKTRRFVNDYLQPPKVTAKMEIGQWRFAEIRED
HHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
LETRMVARDSHELMRALEASSILDCAEMAAHASLYRTESRWGLYHNRTDFPEKNDAEWFC
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
HTILRKVEGRMVSEKRPITPYIVPIEADERGAYDRQRVRKQA
HHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SAAQDAIQDATSEVACDVLVIGGGTAGPMAALKAKLRNPAARVILLEKANVKRSGAISM
CHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEE
GMDGLNNAVIPGYATPEQYTKEITIANDGICDQAPVYKYAENCFEIIQELDRFGIRFQRD
CCCCCCCCCCCCCCCHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEC
GNGDYDVKKVHHLGTYVLPMPNGDTVKKALYRQLRKAQVLISNRFMATRLLNAPDGAVAG
CCCCCHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEE
AVAVNTRTAEFLVIRAKAVILCLGAAGRLGLPHSGYLWGTYENAANSGDGYSMAYHAGAG
EEEECCCCEEEEEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCC
LANLECFQINPLIKDYNGPACAYVAGPFGAYTANSEGNRFIECDYWSGQMMQEFYNELSS
CCCEEEEEECCHHHCCCCCEEEEEECCCCCEECCCCCCEEEEEECCCCHHHHHHHHHHHC
GKGPVFLKLDHLHADTVSEIETILHKVERPSRGRFHENRGTDYRSRMVEMHISEVGFCSG
CCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
HSASGVFVDEFARTTVPGLYSAGDMANVPHNYMLGAFTNGAVAGEHAIDHAGAIDLPEID
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHCCCCCCCCCCCC
AEAVARERERVLAPTRRADGIPPNQIEYKTRRFVNDYLQPPKVTAKMEIGQWRFAEIRED
HHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
LETRMVARDSHELMRALEASSILDCAEMAAHASLYRTESRWGLYHNRTDFPEKNDAEWFC
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
HTILRKVEGRMVSEKRPITPYIVPIEADERGAYDRQRVRKQA
HHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]