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The map label for this gene is mexA [H]

Identifier: 218531583

GI number: 218531583

Start: 3845285

End: 3846610

Strand: Reverse

Name: mexA [H]

Synonym: Mchl_3651

Alternate gene names: 218531583

Gene position: 3846610-3845285 (Counterclockwise)

Preceding gene: 218531584

Following gene: 218531582

Centisome position: 66.57

GC content: 70.21

Gene sequence:

>1326_bases
GTGACGCCGTCTCCCCAGTCGCCCGGCAGCAATCCCGGCCTTGCCAAGGCCGGAAGCCGCCGCTCCCGCTGGTCCCTGCC
GGCCTCTCTTCTCGTCGGTGCCGTCCTCGTCGCCGTCTCGGCCTGCAACGAGCAGAAAGCGGCCGCGCCCGTGCCGCTGC
CGCCGCCCGAGGTCAGCGTCGTCAGCGTCCGTCCGCAACCGATCCCCTACGTCCGTGACCTGCCCGGCCGCGTTGCGCCG
ATGCGCATCGCCGAGGTGCGCTCGCGGGTCTCGGGTCTGGTGATCCGGCGCCTGTTCGAGCAGGGCAGCCACGTCAACGA
GGGCGACGTCCTCTACAAGATCGACCCGGCCCCCTATCAGGTCGACCTCGCCAGCGCCGAGGCGACTTTGGCCAACCGCG
AGGCCGCCCTGGTGCTGGCCAACCAGCAGGCCGACCGCCTCGAGACCCTGCTCGCCCGCAACACCGCGAGCCAGGCGCAG
TACGACACGGCGTTTGCCGCCAAGAAGCAGGCCGAGGCCGAAGTCGCCGGCGCCAAGGCGGCCCGCGATCGGGCGCGGCT
CAACCTGTCCTGGACCGATGTACGCGCGCCGATCTCCGGCCGCATCGGCCGGGCGCTGCTCACCGAGGGCACGCTGATCG
AGTCCGGCATCACCGGCGTGCTCGCCACGATCCAGCAGCTCGACCCGATCTACGTCGACATCACCCAGTCGGTGGGCGAG
CTGAACAAGCTGCGCCGCGACATCGCCTCGGGCGAACTCGCCAAGCTCGCCGCCGACACCGCCAACGTCCACCTCATCAT
GGACGACGGCACGCTCTACGGCTCGGCCGGGCGCCTGCTGTTCTCCGACGTCACCGCCGACCCGAGCACGGGCCAGGTGA
CGCTGCGGGTGCAGTTCCCCAACCCGCACAACGAGCTGTTTCCCGGCATGTATGTCCGCGCCCGGATCAAGCAGGGCATC
GACTCGGACGCCATCGCCGTGCCGCAGCAGGCGATCCAGCGCACCAGTGACGGCAAGCCCGAGGTCTGGGTGGTCAAGCC
CGACGGCAAGGTCATGCTTCAGCCGGTTGAGGTCGGCCCCGTGGTCGGCGGCAACTGGCTGATCCGCGAGGGCCTGGAGG
GCGGCGAGAAGGTCGTCGTCGAAGGCTTCCAGAAGATCTCCGCGGGCATCCAGGTGAAGACCGTCCCGTGGAAGACGGAG
ACCGCTGCCGACGAGGGCAAGCCGATCGACACCGACAGTCACGACACCGACGGCAAGGAGACGAACGAGAAGAGTTCGTC
CGCGGCCGAGCCGGCCGCCCAGCCCGTCGCCCAGTTCGCCGACTGA

Upstream 100 bases:

>100_bases
GCCGGCCGCGTCACGGCATCGGCTCGAAGGCCAGCACCGGCCTTCGGACAGACGTCGATGCGACAACAAATGCCGAGAGC
ATCGTAGGAGTAACCCCATC

Downstream 100 bases:

>100_bases
TTCTTTCTCAAGCATCGCGCGGCCGGCGGGCGTCCCGACGCCCCGCCGGCCCGATGGCATAGGACATCAACGCGCCTCAT
GGCTCGCTTCTTCATCGACC

Product: efflux transporter RND family, MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTPSPQSPGSNPGLAKAGSRRSRWSLPASLLVGAVLVAVSACNEQKAAAPVPLPPPEVSVVSVRPQPIPYVRDLPGRVAP
MRIAEVRSRVSGLVIRRLFEQGSHVNEGDVLYKIDPAPYQVDLASAEATLANREAALVLANQQADRLETLLARNTASQAQ
YDTAFAAKKQAEAEVAGAKAARDRARLNLSWTDVRAPISGRIGRALLTEGTLIESGITGVLATIQQLDPIYVDITQSVGE
LNKLRRDIASGELAKLAADTANVHLIMDDGTLYGSAGRLLFSDVTADPSTGQVTLRVQFPNPHNELFPGMYVRARIKQGI
DSDAIAVPQQAIQRTSDGKPEVWVVKPDGKVMLQPVEVGPVVGGNWLIREGLEGGEKVVVEGFQKISAGIQVKTVPWKTE
TAADEGKPIDTDSHDTDGKETNEKSSSAAEPAAQPVAQFAD

Sequences:

>Translated_441_residues
MTPSPQSPGSNPGLAKAGSRRSRWSLPASLLVGAVLVAVSACNEQKAAAPVPLPPPEVSVVSVRPQPIPYVRDLPGRVAP
MRIAEVRSRVSGLVIRRLFEQGSHVNEGDVLYKIDPAPYQVDLASAEATLANREAALVLANQQADRLETLLARNTASQAQ
YDTAFAAKKQAEAEVAGAKAARDRARLNLSWTDVRAPISGRIGRALLTEGTLIESGITGVLATIQQLDPIYVDITQSVGE
LNKLRRDIASGELAKLAADTANVHLIMDDGTLYGSAGRLLFSDVTADPSTGQVTLRVQFPNPHNELFPGMYVRARIKQGI
DSDAIAVPQQAIQRTSDGKPEVWVVKPDGKVMLQPVEVGPVVGGNWLIREGLEGGEKVVVEGFQKISAGIQVKTVPWKTE
TAADEGKPIDTDSHDTDGKETNEKSSSAAEPAAQPVAQFAD
>Mature_440_residues
TPSPQSPGSNPGLAKAGSRRSRWSLPASLLVGAVLVAVSACNEQKAAAPVPLPPPEVSVVSVRPQPIPYVRDLPGRVAPM
RIAEVRSRVSGLVIRRLFEQGSHVNEGDVLYKIDPAPYQVDLASAEATLANREAALVLANQQADRLETLLARNTASQAQY
DTAFAAKKQAEAEVAGAKAARDRARLNLSWTDVRAPISGRIGRALLTEGTLIESGITGVLATIQQLDPIYVDITQSVGEL
NKLRRDIASGELAKLAADTANVHLIMDDGTLYGSAGRLLFSDVTADPSTGQVTLRVQFPNPHNELFPGMYVRARIKQGID
SDAIAVPQQAIQRTSDGKPEVWVVKPDGKVMLQPVEVGPVVGGNWLIREGLEGGEKVVVEGFQKISAGIQVKTVPWKTET
AADEGKPIDTDSHDTDGKETNEKSSSAAEPAAQPVAQFAD

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=381, Percent_Identity=45.9317585301837, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI1786668, Length=357, Percent_Identity=45.0980392156863, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1789929, Length=389, Percent_Identity=38.0462724935733, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI87082036, Length=354, Percent_Identity=29.9435028248588, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI87081951, Length=148, Percent_Identity=31.0810810810811, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 46938; Mature: 46806

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSPQSPGSNPGLAKAGSRRSRWSLPASLLVGAVLVAVSACNEQKAAAPVPLPPPEVSV
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
VSVRPQPIPYVRDLPGRVAPMRIAEVRSRVSGLVIRRLFEQGSHVNEGDVLYKIDPAPYQ
EEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEE
VDLASAEATLANREAALVLANQQADRLETLLARNTASQAQYDTAFAAKKQAEAEVAGAKA
EEECCCHHHHCCCCEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARDRARLNLSWTDVRAPISGRIGRALLTEGTLIESGITGVLATIQQLDPIYVDITQSVGE
HHHHHEEEEEHHHCCCCCCCCCCHHHHCCCCEECCCHHHHHHHHHHCCCEEEEHHHHHHH
LNKLRRDIASGELAKLAADTANVHLIMDDGTLYGSAGRLLFSDVTADPSTGQVTLRVQFP
HHHHHHHHHCCHHHHHHCCCCEEEEEEECCEEECCCCCEEEEECCCCCCCCEEEEEEECC
NPHNELFPGMYVRARIKQGIDSDAIAVPQQAIQRTSDGKPEVWVVKPDGKVMLQPVEVGP
CCCHHCCCCHHHHHHHHCCCCCCHHHCCHHHHHHCCCCCCCEEEECCCCCEEEEEEEECC
VVGGNWLIREGLEGGEKVVVEGFQKISAGIQVKTVPWKTETAADEGKPIDTDSHDTDGKE
EECCCHHEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
TNEKSSSAAEPAAQPVAQFAD
CCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TPSPQSPGSNPGLAKAGSRRSRWSLPASLLVGAVLVAVSACNEQKAAAPVPLPPPEVSV
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
VSVRPQPIPYVRDLPGRVAPMRIAEVRSRVSGLVIRRLFEQGSHVNEGDVLYKIDPAPYQ
EEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEE
VDLASAEATLANREAALVLANQQADRLETLLARNTASQAQYDTAFAAKKQAEAEVAGAKA
EEECCCHHHHCCCCEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARDRARLNLSWTDVRAPISGRIGRALLTEGTLIESGITGVLATIQQLDPIYVDITQSVGE
HHHHHEEEEEHHHCCCCCCCCCCHHHHCCCCEECCCHHHHHHHHHHCCCEEEEHHHHHHH
LNKLRRDIASGELAKLAADTANVHLIMDDGTLYGSAGRLLFSDVTADPSTGQVTLRVQFP
HHHHHHHHHCCHHHHHHCCCCEEEEEEECCEEECCCCCEEEEECCCCCCCCEEEEEEECC
NPHNELFPGMYVRARIKQGIDSDAIAVPQQAIQRTSDGKPEVWVVKPDGKVMLQPVEVGP
CCCHHCCCCHHHHHHHHCCCCCCHHHCCHHHHHHCCCCCCCEEEECCCCCEEEEEEEECC
VVGGNWLIREGLEGGEKVVVEGFQKISAGIQVKTVPWKTETAADEGKPIDTDSHDTDGKE
EECCCHHEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
TNEKSSSAAEPAAQPVAQFAD
CCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]