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The map label for this gene is fadJ [H]

Identifier: 218531497

GI number: 218531497

Start: 3750961

End: 3753162

Strand: Reverse

Name: fadJ [H]

Synonym: Mchl_3565

Alternate gene names: 218531497

Gene position: 3753162-3750961 (Counterclockwise)

Preceding gene: 218531499

Following gene: 218531496

Centisome position: 64.96

GC content: 68.35

Gene sequence:

>2202_bases
ATGACCGCAACCGAGAATTTCCGCTTCGAGACCGATGCCGACGGCATCGCGCTCGCGACCTGGGACATGCCGGGCCGTTC
GATGAACGTCATCACCATGGAGGTGATGGCGGAACTGGAGCGGATCGTCGACACGGTCGTCGCCGACGAGGCCATCAAGG
GCTGCGTCATCGTCTCGGGGAAGAACAACTTCTCCGGCGGCGCCGACCTCACCATGCTTCAGGGCCTCGGGGTCGAGTAC
GAGCGGCTGAAGGCGAGTGAGGGCGAGGAGGTGGCCATGCGCCACTTCTTCGAGGAGTCGCGGCGCCTCAGCCTCGTGTT
CCGCAAGCTCGAGACGTGCGGCAAGCCGTTCGCGGCGGCGGTGCACGGCCTGTGCCTTGGCGGCGCCTTCGAGCTGGCGC
TGGCCTGCCACCACCGGGTGCTCGCCGACGACGACAAGACCCGCGTCGGCCTGCCCGAGATCAAGGTCGGCCTGTTCCCC
GGCGGCGGCGGCACGCAGCGCGTGTCCCGTCTGATGCAGACCGGCGACGCGCTGCAGATGCTGTTCAAGGGCGAGCAGAT
CCGCGCGCTCATGGCCAAGAACATGGGGCTGGCCCACGCCGTCGCCCCGGCCGCGGAGATCGTCGAGCGGGCCAAGGCCT
GGATCCGCGAGGGCGGCTCGGCGGTGGCACCCTGGGACGTGCCGAAGTTCAAGGCGCCGTCCGGAAAGGTCTACTCGCCC
GCCGGCATGATGATCTGGCCGCCGGCCAACGCGATCTATCGCCGCGAGACCCACGACAATTATCCGGCCGCCAAGGCGAT
CCTGGCCTCGGTCTACGAGGGCCTGCAGCTGCCGATGGATCTCGGCCTCCGGGTCGAGAGCCGCTACTTCGCCCATATCC
TGCGCACCAAGGAAGCGCAGGCGATGATCCGCACCCTGTTCATCTCCATGGGTGAGATCAACAAGGGCGCCCGCCGCCCG
AAGGATGTGGAGGCGACCAAGGTCGGCAAGGTCGGCGTGATCGGCGCCGGCTTCATGGGCGCGGGTATCGCCTACGTCAC
GGCGCAGGCCGGGATGCAGGTCGTGCTGATCGACCGCGATCAGGAGGCGGCCGATGCCGGCAAGGCGCATTGCCACAAGC
TCATCACCGGCCAGATCAACCGCGGCAAGGCCAAGACCGCTGACCGCGACGCGCTGCTCGCCCGCATCACCGCCACCCCC
GATTACGGTGCCCTGTCCGATTGCGACCTCGTGATCGAGGCGGTGTTCGAAGACCCTAAGGTCAAGGCCGAGGTGATCGC
CAAGGTCGAGGCGGCGCTGCCGGCGCACGCGATCTTCGCCTCCAACACCTCGACCCTGCCGATCTCCGGCCTCGCCCAGA
CCTCGAAGCGGCCCGAGCAGTTCGTCGGCATCCACTTCTTCTCGCCGGTGGAGAAGATGCTGCTCGTGGAGATCATCCGC
GGCAACCAGACCGGAGACCGCGCGGTCGCCACCGCCCTCGACTACGTGCGCGCGATCAAGAAGACCGGCATCGTCGTCAA
CGATTCCCGCGGCTTCTTCGCCAACCGCTGCGTGCTCGCCTACATCCATGAGGGCCACAAGATGCTCACCGAGGGCGTGC
CCCCGGCGATGATCGAGAACGTGGCGCGGATGGCCGGCATGCCGGTCGGCCCGCTCTCGCTCAACGACGAGGTGGCGCTC
GATCTCGGCCTCAAGATCATGCGGGCGACCGAGGCGCAGCTCGGCGAGGGCGCTGTCGATCCGGACCAGAAGCGGCTCCT
CGTCGCGATGGTCGAGACGCATGGCCGGCTCGGGCGTAAGAACAAGCGCGGCTTCTACGACTACCCCGAGGGCGGCCAGA
AGACGCTCTGGCCGGGGCTCGCCGAGATCCAGCCGAACCGGCTCGACCCGGAGAGCATCGACATCGACGAGCTGAAGCAC
CGCTTCCTCGTGGTGCAGGCGCTGGAGGCCGCCCGCACGATGCAGGAGGGCGTGGTGACCGATCCGCGCGAGGCGGATGT
CGGCTCGATCCTCGGCTTCGGCTTCGCCCCCTTCACCGGCGGCGCGCTCTCCTACATCGACTTCATGGGCGCCAAGGCCT
TCGTGGCGCTGGCACGTGAGCTCGAAGCCCGGCACGGCGCCCGCTTCACCCCGCCGGACAACCTCCTGGCGATGGCCGAG
TCCGGGGCGCGCTTCTACGACGCGCTGAAGCAGGCGGCGTAG

Upstream 100 bases:

>100_bases
GGAGAGCCGGGTCCAGTCGCGCACCCGTTGCATCCCCCATGACGGCCCCCTCCCCTTAAGAATCCGATACGGCCAGGCTC
TTTTCCTTCGAGGCTCCGCG

Downstream 100 bases:

>100_bases
CGATCAGGCTGGCCCTCCCTGAGTGGGAGGGCCGCCATGCGGAACCGAAAAGCTCCGCTTTCGTTCACCGCCCGACCGCA
CTGAGTGAACCGGAGTTTCC

Product: 3-hydroxyacyl-CoA dehydrogenase NAD-binding

Products: NA

Alternate protein names: Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase [H]

Number of amino acids: Translated: 733; Mature: 732

Protein sequence:

>733_residues
MTATENFRFETDADGIALATWDMPGRSMNVITMEVMAELERIVDTVVADEAIKGCVIVSGKNNFSGGADLTMLQGLGVEY
ERLKASEGEEVAMRHFFEESRRLSLVFRKLETCGKPFAAAVHGLCLGGAFELALACHHRVLADDDKTRVGLPEIKVGLFP
GGGGTQRVSRLMQTGDALQMLFKGEQIRALMAKNMGLAHAVAPAAEIVERAKAWIREGGSAVAPWDVPKFKAPSGKVYSP
AGMMIWPPANAIYRRETHDNYPAAKAILASVYEGLQLPMDLGLRVESRYFAHILRTKEAQAMIRTLFISMGEINKGARRP
KDVEATKVGKVGVIGAGFMGAGIAYVTAQAGMQVVLIDRDQEAADAGKAHCHKLITGQINRGKAKTADRDALLARITATP
DYGALSDCDLVIEAVFEDPKVKAEVIAKVEAALPAHAIFASNTSTLPISGLAQTSKRPEQFVGIHFFSPVEKMLLVEIIR
GNQTGDRAVATALDYVRAIKKTGIVVNDSRGFFANRCVLAYIHEGHKMLTEGVPPAMIENVARMAGMPVGPLSLNDEVAL
DLGLKIMRATEAQLGEGAVDPDQKRLLVAMVETHGRLGRKNKRGFYDYPEGGQKTLWPGLAEIQPNRLDPESIDIDELKH
RFLVVQALEAARTMQEGVVTDPREADVGSILGFGFAPFTGGALSYIDFMGAKAFVALARELEARHGARFTPPDNLLAMAE
SGARFYDALKQAA

Sequences:

>Translated_733_residues
MTATENFRFETDADGIALATWDMPGRSMNVITMEVMAELERIVDTVVADEAIKGCVIVSGKNNFSGGADLTMLQGLGVEY
ERLKASEGEEVAMRHFFEESRRLSLVFRKLETCGKPFAAAVHGLCLGGAFELALACHHRVLADDDKTRVGLPEIKVGLFP
GGGGTQRVSRLMQTGDALQMLFKGEQIRALMAKNMGLAHAVAPAAEIVERAKAWIREGGSAVAPWDVPKFKAPSGKVYSP
AGMMIWPPANAIYRRETHDNYPAAKAILASVYEGLQLPMDLGLRVESRYFAHILRTKEAQAMIRTLFISMGEINKGARRP
KDVEATKVGKVGVIGAGFMGAGIAYVTAQAGMQVVLIDRDQEAADAGKAHCHKLITGQINRGKAKTADRDALLARITATP
DYGALSDCDLVIEAVFEDPKVKAEVIAKVEAALPAHAIFASNTSTLPISGLAQTSKRPEQFVGIHFFSPVEKMLLVEIIR
GNQTGDRAVATALDYVRAIKKTGIVVNDSRGFFANRCVLAYIHEGHKMLTEGVPPAMIENVARMAGMPVGPLSLNDEVAL
DLGLKIMRATEAQLGEGAVDPDQKRLLVAMVETHGRLGRKNKRGFYDYPEGGQKTLWPGLAEIQPNRLDPESIDIDELKH
RFLVVQALEAARTMQEGVVTDPREADVGSILGFGFAPFTGGALSYIDFMGAKAFVALARELEARHGARFTPPDNLLAMAE
SGARFYDALKQAA
>Mature_732_residues
TATENFRFETDADGIALATWDMPGRSMNVITMEVMAELERIVDTVVADEAIKGCVIVSGKNNFSGGADLTMLQGLGVEYE
RLKASEGEEVAMRHFFEESRRLSLVFRKLETCGKPFAAAVHGLCLGGAFELALACHHRVLADDDKTRVGLPEIKVGLFPG
GGGTQRVSRLMQTGDALQMLFKGEQIRALMAKNMGLAHAVAPAAEIVERAKAWIREGGSAVAPWDVPKFKAPSGKVYSPA
GMMIWPPANAIYRRETHDNYPAAKAILASVYEGLQLPMDLGLRVESRYFAHILRTKEAQAMIRTLFISMGEINKGARRPK
DVEATKVGKVGVIGAGFMGAGIAYVTAQAGMQVVLIDRDQEAADAGKAHCHKLITGQINRGKAKTADRDALLARITATPD
YGALSDCDLVIEAVFEDPKVKAEVIAKVEAALPAHAIFASNTSTLPISGLAQTSKRPEQFVGIHFFSPVEKMLLVEIIRG
NQTGDRAVATALDYVRAIKKTGIVVNDSRGFFANRCVLAYIHEGHKMLTEGVPPAMIENVARMAGMPVGPLSLNDEVALD
LGLKIMRATEAQLGEGAVDPDQKRLLVAMVETHGRLGRKNKRGFYDYPEGGQKTLWPGLAEIQPNRLDPESIDIDELKHR
FLVVQALEAARTMQEGVVTDPREADVGSILGFGFAPFTGGALSYIDFMGAKAFVALARELEARHGARFTPPDNLLAMAES
GARFYDALKQAA

Specific function: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities [H]

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI20127408, Length=740, Percent_Identity=33.7837837837838, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI68989263, Length=736, Percent_Identity=27.5815217391304, Blast_Score=248, Evalue=2e-65,
Organism=Homo sapiens, GI261878539, Length=629, Percent_Identity=28.2988871224165, Blast_Score=224, Evalue=2e-58,
Organism=Homo sapiens, GI296179429, Length=289, Percent_Identity=32.1799307958478, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI296179427, Length=306, Percent_Identity=30.3921568627451, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI194097323, Length=186, Percent_Identity=29.5698924731183, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI213417737, Length=213, Percent_Identity=25.8215962441315, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI157694516, Length=213, Percent_Identity=25.8215962441315, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788682, Length=737, Percent_Identity=35.9565807327001, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI1790281, Length=732, Percent_Identity=31.5573770491803, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1787661, Length=288, Percent_Identity=32.6388888888889, Blast_Score=144, Evalue=3e-35,
Organism=Escherichia coli, GI1787659, Length=183, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788597, Length=215, Percent_Identity=27.906976744186, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=760, Percent_Identity=31.8421052631579, Blast_Score=351, Evalue=8e-97,
Organism=Caenorhabditis elegans, GI17508953, Length=751, Percent_Identity=32.7563249001332, Blast_Score=342, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI17508951, Length=751, Percent_Identity=32.7563249001332, Blast_Score=342, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI25144276, Length=629, Percent_Identity=33.3863275039746, Blast_Score=297, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI71985930, Length=375, Percent_Identity=29.0666666666667, Blast_Score=173, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI71985923, Length=367, Percent_Identity=30.2452316076294, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17549919, Length=294, Percent_Identity=34.3537414965986, Blast_Score=153, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17553560, Length=290, Percent_Identity=31.3793103448276, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17563036, Length=310, Percent_Identity=29.6774193548387, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25145438, Length=199, Percent_Identity=30.6532663316583, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17554946, Length=200, Percent_Identity=28, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=219, Percent_Identity=28.7671232876712, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI19921000, Length=750, Percent_Identity=32.6666666666667, Blast_Score=325, Evalue=5e-89,
Organism=Drosophila melanogaster, GI24583077, Length=750, Percent_Identity=32.6666666666667, Blast_Score=325, Evalue=6e-89,
Organism=Drosophila melanogaster, GI24583079, Length=750, Percent_Identity=32.6666666666667, Blast_Score=325, Evalue=6e-89,
Organism=Drosophila melanogaster, GI20129971, Length=200, Percent_Identity=28.5, Blast_Score=87, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24653477, Length=200, Percent_Identity=28.5, Blast_Score=87, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24653139, Length=200, Percent_Identity=29, Blast_Score=72, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006180
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR001753
- InterPro:   IPR013328
- InterPro:   IPR012802
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; PF00378 ECH [H]

EC number: =4.2.1.17; =5.1.2.3; =1.1.1.35 [H]

Molecular weight: Translated: 79298; Mature: 79166

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00067 3HCDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATENFRFETDADGIALATWDMPGRSMNVITMEVMAELERIVDTVVADEAIKGCVIVSG
CCCCCCCEEECCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
KNNFSGGADLTMLQGLGVEYERLKASEGEEVAMRHFFEESRRLSLVFRKLETCGKPFAAA
CCCCCCCCCCHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VHGLCLGGAFELALACHHRVLADDDKTRVGLPEIKVGLFPGGGGTQRVSRLMQTGDALQM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCHHHHH
LFKGEQIRALMAKNMGLAHAVAPAAEIVERAKAWIREGGSAVAPWDVPKFKAPSGKVYSP
HHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECC
AGMMIWPPANAIYRRETHDNYPAAKAILASVYEGLQLPMDLGLRVESRYFAHILRTKEAQ
CCCEEECCCCHHEECCCCCCCHHHHHHHHHHHHHHCCCHHCCCEEHHHHHHHHHHHHHHH
AMIRTLFISMGEINKGARRPKDVEATKVGKVGVIGAGFMGAGIAYVTAQAGMQVVLIDRD
HHHHHHHHHHHCCCCCCCCCCCCCHHCCCCEEEEECCHHHCCHHEEECCCCCEEEEEECC
QEAADAGKAHCHKLITGQINRGKAKTADRDALLARITATPDYGALSDCDLVIEAVFEDPK
CHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEEECCCCCCCCCHHHHHHHHHHCCCC
VKAEVIAKVEAALPAHAIFASNTSTLPISGLAQTSKRPEQFVGIHFFSPVEKMLLVEIIR
HHHHHHHHHHHHCCHHEEEECCCCCCCCCHHHHCCCCHHHEEEEEHHHHHHHHHHHHHHC
GNQTGDRAVATALDYVRAIKKTGIVVNDSRGFFANRCVLAYIHEGHKMLTEGVPPAMIEN
CCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHEEEEEHHHCHHHHHCCCCHHHHHH
VARMAGMPVGPLSLNDEVALDLGLKIMRATEAQLGEGAVDPDQKRLLVAMVETHGRLGRK
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEHHHHHHHHHCCCC
NKRGFYDYPEGGQKTLWPGLAEIQPNRLDPESIDIDELKHRFLVVQALEAARTMQEGVVT
CCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DPREADVGSILGFGFAPFTGGALSYIDFMGAKAFVALARELEARHGARFTPPDNLLAMAE
CCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
SGARFYDALKQAA
CCHHHHHHHHHCC
>Mature Secondary Structure 
TATENFRFETDADGIALATWDMPGRSMNVITMEVMAELERIVDTVVADEAIKGCVIVSG
CCCCCCEEECCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
KNNFSGGADLTMLQGLGVEYERLKASEGEEVAMRHFFEESRRLSLVFRKLETCGKPFAAA
CCCCCCCCCCHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VHGLCLGGAFELALACHHRVLADDDKTRVGLPEIKVGLFPGGGGTQRVSRLMQTGDALQM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCHHHHH
LFKGEQIRALMAKNMGLAHAVAPAAEIVERAKAWIREGGSAVAPWDVPKFKAPSGKVYSP
HHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECC
AGMMIWPPANAIYRRETHDNYPAAKAILASVYEGLQLPMDLGLRVESRYFAHILRTKEAQ
CCCEEECCCCHHEECCCCCCCHHHHHHHHHHHHHHCCCHHCCCEEHHHHHHHHHHHHHHH
AMIRTLFISMGEINKGARRPKDVEATKVGKVGVIGAGFMGAGIAYVTAQAGMQVVLIDRD
HHHHHHHHHHHCCCCCCCCCCCCCHHCCCCEEEEECCHHHCCHHEEECCCCCEEEEEECC
QEAADAGKAHCHKLITGQINRGKAKTADRDALLARITATPDYGALSDCDLVIEAVFEDPK
CHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEEECCCCCCCCCHHHHHHHHHHCCCC
VKAEVIAKVEAALPAHAIFASNTSTLPISGLAQTSKRPEQFVGIHFFSPVEKMLLVEIIR
HHHHHHHHHHHHCCHHEEEECCCCCCCCCHHHHCCCCHHHEEEEEHHHHHHHHHHHHHHC
GNQTGDRAVATALDYVRAIKKTGIVVNDSRGFFANRCVLAYIHEGHKMLTEGVPPAMIEN
CCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHEEEEEHHHCHHHHHCCCCHHHHHH
VARMAGMPVGPLSLNDEVALDLGLKIMRATEAQLGEGAVDPDQKRLLVAMVETHGRLGRK
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEHHHHHHHHHCCCC
NKRGFYDYPEGGQKTLWPGLAEIQPNRLDPESIDIDELKHRFLVVQALEAARTMQEGVVT
CCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DPREADVGSILGFGFAPFTGGALSYIDFMGAKAFVALARELEARHGARFTPPDNLLAMAE
CCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
SGARFYDALKQAA
CCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA