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The map label for this gene is slt [H]

Identifier: 218531446

GI number: 218531446

Start: 3698169

End: 3700493

Strand: Reverse

Name: slt [H]

Synonym: Mchl_3514

Alternate gene names: 218531446

Gene position: 3700493-3698169 (Counterclockwise)

Preceding gene: 218531449

Following gene: 218531444

Centisome position: 64.05

GC content: 72.13

Gene sequence:

>2325_bases
ATGGCGTTGCCCACACGCTCCCTGCCTCTGCTCCTCGGTCTGTTGCTGTCCGGCACGGCCGTGCTGCCACAGCGCGGCGG
GGCCATCGAACTCCGCAACCCGCAGCCGGCCTCCGCCCCCACCGCCCCCGCGAGCGACGCTACCGCGCCCGCCGGGCAGC
CGAGCGACGGCCAGGGCCCTGCCTCGGATCCCGTCGCGGCGGATGCGCTGCGCCTCGATCCGAGTTCCGGCGAGGCCGCC
TCCGACAAGCCGCTGCCCGCCGCGGCGTCCTCCTACGCTTCCACCGAATCGGATGGCCCTGTCGCCTTCCCGCTGCCCGA
TGCGGCCGTGTCGCCGGCCGCCCCCTCCCCTGAAGTGCCGGACCCGGCCCGGCCGGTCGCCTTTGGCGAGACCGATCTCG
GCCAACTCCGCGAGACGATCGACCTCTACCGCAAGGGCAAGGTCTCCGACGGCGACCGGCTCGCCGCAGGCTTCTCCGAT
CCGGCGGCGCGGGCGCTGCTCGAATGGGTCGCGATCCGTGCGGGCGCCGGCGTCAGCTTCGAGCGGGTCGTCGCCTTCTC
GCGCGCCAATCCCGATTGGCCGGCGGGGCCCCTGCTGCGCCGCCGGGCCGAGGAGCGGCTGCTCACGGAGAAGAAGAGCC
CTGCGATCGTGCGCGCCTATTTCGCGAGCGCCAAGCCGGCGAGTGCGCCGGGCAAGTTCGCCCTGGCCCTGGCCCTGCGC
GCCGACGGGCTCGACGCGGATGCCGCCGAACTCGTGCATGACCTGTGGCGTGAGGAGAGCTTCGGGCGCAGCCTTGAGGC
CAAGGTCACCGACGCCTTCCCCGGCGTGCTCACGGTGATCGACCACCGCTACCGGATGGAGCGCGCCCTCCTGAAGGAGG
ATTGGGCCACCGCCGCGCGCGCGGCCGACTATGCCGGCGGCTCCTATGCCAGCCTCGTGCGCGCCCGCCGGGCGGTGCAG
GGCAAGGCCGGGGCGGCGGCCGCGCTCGCGGCGGTGCCGCCGTCGTTGCGCAACGATTCGTCCTACCTGCTCTCGCGCGC
CCAGTATTTCCGCCGCGCCGACAAGGCGCCGGAGGCGGCCAAGGTGCTGCTCGCCGCGCCGACCAACCCGGAGGTTCTCG
CCGACGGTGACACGTGGTGGGTCGAGCGCCGGATCGTCGCCCGCAAGCTGATGGATGCGGGCGAGACCAAGACCGCCTAC
GCCGTGGCCGCCGCGCATTCCGCCCGCACGTCCGAGAAGCGGATCGAAGCCGAGTTCCATGCCGGCTGGATCGCGCTCCG
CTTCATGGCCGACCCCGCCGCGGCTGAGCGGCACTTCGCGCAAGCCGCGAGTGTCGCCGAGACGCCGATCTCGCTGGCGC
GAGCCGCCTATTGGCAGGGCCGCGCGGCGGAAGCCGCGGGCAAGATCATCGAATCGAAGAGCTTCTACGAGCGCGCCGCG
CTCCAGCCGATCGCCTATTACGGGCAGCTCGCCCGTGCCCGGCTCGGCCAGCACAGCCTGCCCCTGCGCCGCGCGGCCGA
GCTGAGCCCGACGGCACGCGCCGCCTTCGACGACCGGCTCTTCATGCGCGGCCTGAAGCTTCTGGCCGCCGCCCAGATGA
AGGACTTGGCGCTGCCGCTCTACATCGACGCCGCCCGCTCGCTGACCGAGGAGGCGCAGGTCAACGCGCTCGGTGAGACG
GCGGTGGAGATGCGCGATGCGCGGGCGCTGGTCTCCATCGGTAAGCTCGCCACGCAGCGGGGGCTGGCACTGGACACCCA
TGCCTACCCGACGATCGGCATCCCGAATTACGAGACCTTCACCGCGGTGCCGCAGGTCGAGCGGGCCATGGTCTTCGCCA
TCGCCCGGCAGGAAAGCCAGTTCGATCCCCGCGCCCAGTCCGGCGTCGGCGCCAGGGGGCTGATGCAGATGATGCCGGCC
ACCGCCCAGCGCACCGCCAAGCGCGTCTCGACGTCCTATGAAACGGACCGGCTCACCAGCGATCCCGCCTACAACGCGCG
GCTGGGCCAAGCCCATCTCGGCGAGCTGATGGAGGACTGGAAGGGCTCCTACATCCTCGCCTTCGCCTCCTACAACGCGG
GCGGCGGCAACGTGAAGAAATGGGTCGATGCCTACGGCGACCCGCGCCGGCCGGATGTCGATCCGATCGACTGGGTCGAG
CGCATCCCCTTCACCGAGACGCGCAACTACGTGCAGCGGGTGATGGAGAACCTGCAGGTCTACCGCAACCGCCTCGACGG
CAAGAGTGCGCTGATGATCGAGCGCGACCTCGCCCGCGGCGCCCGCACCGCCGTCAGCGCCGAGGCGCAGCCGGTCACGC
CGTAG

Upstream 100 bases:

>100_bases
CCCCCGTCCGCGCGCAAACGCTTCGCTTGCGCAGTTAATGCCATCTTAACGGACCGAGGCCCAGCTTAAGGCTTGGCCTC
CGGTCGGGGTGTCTCAGATC

Downstream 100 bases:

>100_bases
TGGCGTGACCCTGGTAGTGCCGGTCACACCGTTGTGGCGTGCCTCTGGTAGTGACTGCGGACCAGCGCCTCCACGAAGGG
CCAGAGACCGGGGATGCCGC

Product: lytic transglycosylase catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 774; Mature: 773

Protein sequence:

>774_residues
MALPTRSLPLLLGLLLSGTAVLPQRGGAIELRNPQPASAPTAPASDATAPAGQPSDGQGPASDPVAADALRLDPSSGEAA
SDKPLPAAASSYASTESDGPVAFPLPDAAVSPAAPSPEVPDPARPVAFGETDLGQLRETIDLYRKGKVSDGDRLAAGFSD
PAARALLEWVAIRAGAGVSFERVVAFSRANPDWPAGPLLRRRAEERLLTEKKSPAIVRAYFASAKPASAPGKFALALALR
ADGLDADAAELVHDLWREESFGRSLEAKVTDAFPGVLTVIDHRYRMERALLKEDWATAARAADYAGGSYASLVRARRAVQ
GKAGAAAALAAVPPSLRNDSSYLLSRAQYFRRADKAPEAAKVLLAAPTNPEVLADGDTWWVERRIVARKLMDAGETKTAY
AVAAAHSARTSEKRIEAEFHAGWIALRFMADPAAAERHFAQAASVAETPISLARAAYWQGRAAEAAGKIIESKSFYERAA
LQPIAYYGQLARARLGQHSLPLRRAAELSPTARAAFDDRLFMRGLKLLAAAQMKDLALPLYIDAARSLTEEAQVNALGET
AVEMRDARALVSIGKLATQRGLALDTHAYPTIGIPNYETFTAVPQVERAMVFAIARQESQFDPRAQSGVGARGLMQMMPA
TAQRTAKRVSTSYETDRLTSDPAYNARLGQAHLGELMEDWKGSYILAFASYNAGGGNVKKWVDAYGDPRRPDVDPIDWVE
RIPFTETRNYVQRVMENLQVYRNRLDGKSALMIERDLARGARTAVSAEAQPVTP

Sequences:

>Translated_774_residues
MALPTRSLPLLLGLLLSGTAVLPQRGGAIELRNPQPASAPTAPASDATAPAGQPSDGQGPASDPVAADALRLDPSSGEAA
SDKPLPAAASSYASTESDGPVAFPLPDAAVSPAAPSPEVPDPARPVAFGETDLGQLRETIDLYRKGKVSDGDRLAAGFSD
PAARALLEWVAIRAGAGVSFERVVAFSRANPDWPAGPLLRRRAEERLLTEKKSPAIVRAYFASAKPASAPGKFALALALR
ADGLDADAAELVHDLWREESFGRSLEAKVTDAFPGVLTVIDHRYRMERALLKEDWATAARAADYAGGSYASLVRARRAVQ
GKAGAAAALAAVPPSLRNDSSYLLSRAQYFRRADKAPEAAKVLLAAPTNPEVLADGDTWWVERRIVARKLMDAGETKTAY
AVAAAHSARTSEKRIEAEFHAGWIALRFMADPAAAERHFAQAASVAETPISLARAAYWQGRAAEAAGKIIESKSFYERAA
LQPIAYYGQLARARLGQHSLPLRRAAELSPTARAAFDDRLFMRGLKLLAAAQMKDLALPLYIDAARSLTEEAQVNALGET
AVEMRDARALVSIGKLATQRGLALDTHAYPTIGIPNYETFTAVPQVERAMVFAIARQESQFDPRAQSGVGARGLMQMMPA
TAQRTAKRVSTSYETDRLTSDPAYNARLGQAHLGELMEDWKGSYILAFASYNAGGGNVKKWVDAYGDPRRPDVDPIDWVE
RIPFTETRNYVQRVMENLQVYRNRLDGKSALMIERDLARGARTAVSAEAQPVTP
>Mature_773_residues
ALPTRSLPLLLGLLLSGTAVLPQRGGAIELRNPQPASAPTAPASDATAPAGQPSDGQGPASDPVAADALRLDPSSGEAAS
DKPLPAAASSYASTESDGPVAFPLPDAAVSPAAPSPEVPDPARPVAFGETDLGQLRETIDLYRKGKVSDGDRLAAGFSDP
AARALLEWVAIRAGAGVSFERVVAFSRANPDWPAGPLLRRRAEERLLTEKKSPAIVRAYFASAKPASAPGKFALALALRA
DGLDADAAELVHDLWREESFGRSLEAKVTDAFPGVLTVIDHRYRMERALLKEDWATAARAADYAGGSYASLVRARRAVQG
KAGAAAALAAVPPSLRNDSSYLLSRAQYFRRADKAPEAAKVLLAAPTNPEVLADGDTWWVERRIVARKLMDAGETKTAYA
VAAAHSARTSEKRIEAEFHAGWIALRFMADPAAAERHFAQAASVAETPISLARAAYWQGRAAEAAGKIIESKSFYERAAL
QPIAYYGQLARARLGQHSLPLRRAAELSPTARAAFDDRLFMRGLKLLAAAQMKDLALPLYIDAARSLTEEAQVNALGETA
VEMRDARALVSIGKLATQRGLALDTHAYPTIGIPNYETFTAVPQVERAMVFAIARQESQFDPRAQSGVGARGLMQMMPAT
AQRTAKRVSTSYETDRLTSDPAYNARLGQAHLGELMEDWKGSYILAFASYNAGGGNVKKWVDAYGDPRRPDVDPIDWVER
IPFTETRNYVQRVMENLQVYRNRLDGKSALMIERDLARGARTAVSAEAQPVTP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=158, Percent_Identity=36.0759493670886, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 83050; Mature: 82918

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00922 TRANSGLYCOSYLASE ; PS00205 TRANSFERRIN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPTRSLPLLLGLLLSGTAVLPQRGGAIELRNPQPASAPTAPASDATAPAGQPSDGQGP
CCCCCCHHHHHHHHHHCCCEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ASDPVAADALRLDPSSGEAASDKPLPAAASSYASTESDGPVAFPLPDAAVSPAAPSPEVP
CCCCCHHHHEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCC
DPARPVAFGETDLGQLRETIDLYRKGKVSDGDRLAAGFSDPAARALLEWVAIRAGAGVSF
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCH
ERVVAFSRANPDWPAGPLLRRRAEERLLTEKKSPAIVRAYFASAKPASAPGKFALALALR
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCCCCCEEEEEEEE
ADGLDADAAELVHDLWREESFGRSLEAKVTDAFPGVLTVIDHRYRMERALLKEDWATAAR
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AADYAGGSYASLVRARRAVQGKAGAAAALAAVPPSLRNDSSYLLSRAQYFRRADKAPEAA
HHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHH
KVLLAAPTNPEVLADGDTWWVERRIVARKLMDAGETKTAYAVAAAHSARTSEKRIEAEFH
EEEEECCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AGWIALRFMADPAAAERHFAQAASVAETPISLARAAYWQGRAAEAAGKIIESKSFYERAA
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LQPIAYYGQLARARLGQHSLPLRRAAELSPTARAAFDDRLFMRGLKLLAAAQMKDLALPL
HHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YIDAARSLTEEAQVNALGETAVEMRDARALVSIGKLATQRGLALDTHAYPTIGIPNYETF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHH
TAVPQVERAMVFAIARQESQFDPRAQSGVGARGLMQMMPATAQRTAKRVSTSYETDRLTS
CCCHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCC
DPAYNARLGQAHLGELMEDWKGSYILAFASYNAGGGNVKKWVDAYGDPRRPDVDPIDWVE
CCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCHHHHHH
RIPFTETRNYVQRVMENLQVYRNRLDGKSALMIERDLARGARTAVSAEAQPVTP
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ALPTRSLPLLLGLLLSGTAVLPQRGGAIELRNPQPASAPTAPASDATAPAGQPSDGQGP
CCCCCHHHHHHHHHHCCCEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ASDPVAADALRLDPSSGEAASDKPLPAAASSYASTESDGPVAFPLPDAAVSPAAPSPEVP
CCCCCHHHHEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCC
DPARPVAFGETDLGQLRETIDLYRKGKVSDGDRLAAGFSDPAARALLEWVAIRAGAGVSF
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCH
ERVVAFSRANPDWPAGPLLRRRAEERLLTEKKSPAIVRAYFASAKPASAPGKFALALALR
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCCCCCEEEEEEEE
ADGLDADAAELVHDLWREESFGRSLEAKVTDAFPGVLTVIDHRYRMERALLKEDWATAAR
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AADYAGGSYASLVRARRAVQGKAGAAAALAAVPPSLRNDSSYLLSRAQYFRRADKAPEAA
HHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHH
KVLLAAPTNPEVLADGDTWWVERRIVARKLMDAGETKTAYAVAAAHSARTSEKRIEAEFH
EEEEECCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AGWIALRFMADPAAAERHFAQAASVAETPISLARAAYWQGRAAEAAGKIIESKSFYERAA
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LQPIAYYGQLARARLGQHSLPLRRAAELSPTARAAFDDRLFMRGLKLLAAAQMKDLALPL
HHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YIDAARSLTEEAQVNALGETAVEMRDARALVSIGKLATQRGLALDTHAYPTIGIPNYETF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHH
TAVPQVERAMVFAIARQESQFDPRAQSGVGARGLMQMMPATAQRTAKRVSTSYETDRLTS
CCCHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCC
DPAYNARLGQAHLGELMEDWKGSYILAFASYNAGGGNVKKWVDAYGDPRRPDVDPIDWVE
CCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCHHHHHH
RIPFTETRNYVQRVMENLQVYRNRLDGKSALMIERDLARGARTAVSAEAQPVTP
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]