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The map label for this gene is ccrM [H]

Identifier: 218531437

GI number: 218531437

Start: 3687166

End: 3688362

Strand: Reverse

Name: ccrM [H]

Synonym: Mchl_3505

Alternate gene names: 218531437

Gene position: 3688362-3687166 (Counterclockwise)

Preceding gene: 218531444

Following gene: 218531435

Centisome position: 63.84

GC content: 68.09

Gene sequence:

>1197_bases
ATGGCTTCCCCGCGTACCGCGGTCGTCGGCGCCACCGCCCGGAATCAAGTCTCGCGTACCGGGCGGATCGCGTCGGCGCC
GCGGATGGGTCTTGCACCCTCCGTTCAGCGTCTTCCCAGCACCTTACCCCTCGACGAAATCCTGATCGGCGACTGTATCG
CCGCGATGGATCGTCTGCCGGCGGAAAGCGTCGACTGTGTCTTCGCCGACCCGCCCTACAACCTCCAGCTCGGCGAGGCG
GGATTGACCCGTCCCGACCAGAGCGTGGTCGATGCCGTCGACGACGACTGGGACAAGTTCTCCAGCTTTTCCGCCTATGA
CGACTTCACCCGCGCCTGGCTGAAGGCCGCGCGCCGCGTGATGAAGCCGAACGCCACGCTGTGGGTGATCGGTTCGTATC
ACAACATCTTCCGGGTCGGCAGCGCGTTGCAGGATCTCGGTTACTGGATCCTGAACGACATCGTCTGGCGCAAGGCCAAC
CCGATGCCGAACTTCCGCGGCAAGCGCTTCACCAACGCGCATGAGACCCTGATCTGGGCCTCGCGCTCGCCACAGGCGAA
GTACACCTTCCATTACGATGCTCTGAAGGCCGGCAACGAAGACCTTCAGATGCGCTCGGACTGGTTCATTCCGCTCTGCA
CGGGCGAGGAGCGGCTGAAGGACGAGGCGGGCCGCAAGGTCCACCCGACCCAGAAGCCGGAAGCGCTCCTGGCCCGCACG
CTGCTGGCGGCGACCAATCCCGGCGACGTGGTGCTCGACCCGTTCTTCGGCACCGGCACGACCGGCGCGGTGGCCAAGCG
CCTTGGCCGCCACTTCATCGGCTGCGAGCGCGACCCGACCTACGCCACCGCGGCGCGGGCTCGGATCGACGGTATCGAGA
CGCTGTCGGCCGCCTCGCTGGCGCTCGCGACGCCGAAGCGGTCGGAGCCGCGCATCCCGTTCCTGAGCGTGGTCGAAGCG
GGCCATGTCCGCCCCGGCGAGACGCTGACCGACGAGCGCCGCCGCTTCCGGGCGACGGTGCGGCCGGACGGCCAGCTCAG
CGTCGGGCCGGCGATGGGCTCGATCCACAAGGTCGGCGCCCTGGTGCAGGGCCTGCCCGCCTGCAACGGCTGGACCTTCT
GGCACGCCGAGCGCAGCGGAAAACTCGTCTGCATCGACGATTTTCGGACTGAGATGCGCGGCCAAGCCGGGGCGTAG

Upstream 100 bases:

>100_bases
GCCGCCCCATATCGAGACGCAACCGTACGGCGACGGCAAACGCGACCTTTACCGTGATCGGGCCATGGTCCGGTTGTCAG
TCGCGTAACCGGTGTTTGCC

Downstream 100 bases:

>100_bases
CGCTCGTCGCGGTTTCGAAAGGACAAGTCCTTTCGCGGGTGCAGGGCAGAGCCCTGCATCTTTCTATCGTGTCCGAATCG
GCTTGGGCGCCTTCTTCACG

Product: DNA methylase N-4/N-6 domain protein

Products: NA

Alternate protein names: M.BabI; Adenine-specific methyltransferase BabI; DNA methyltransferase CcrM; M.CcrMI ortholog [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MASPRTAVVGATARNQVSRTGRIASAPRMGLAPSVQRLPSTLPLDEILIGDCIAAMDRLPAESVDCVFADPPYNLQLGEA
GLTRPDQSVVDAVDDDWDKFSSFSAYDDFTRAWLKAARRVMKPNATLWVIGSYHNIFRVGSALQDLGYWILNDIVWRKAN
PMPNFRGKRFTNAHETLIWASRSPQAKYTFHYDALKAGNEDLQMRSDWFIPLCTGEERLKDEAGRKVHPTQKPEALLART
LLAATNPGDVVLDPFFGTGTTGAVAKRLGRHFIGCERDPTYATAARARIDGIETLSAASLALATPKRSEPRIPFLSVVEA
GHVRPGETLTDERRRFRATVRPDGQLSVGPAMGSIHKVGALVQGLPACNGWTFWHAERSGKLVCIDDFRTEMRGQAGA

Sequences:

>Translated_398_residues
MASPRTAVVGATARNQVSRTGRIASAPRMGLAPSVQRLPSTLPLDEILIGDCIAAMDRLPAESVDCVFADPPYNLQLGEA
GLTRPDQSVVDAVDDDWDKFSSFSAYDDFTRAWLKAARRVMKPNATLWVIGSYHNIFRVGSALQDLGYWILNDIVWRKAN
PMPNFRGKRFTNAHETLIWASRSPQAKYTFHYDALKAGNEDLQMRSDWFIPLCTGEERLKDEAGRKVHPTQKPEALLART
LLAATNPGDVVLDPFFGTGTTGAVAKRLGRHFIGCERDPTYATAARARIDGIETLSAASLALATPKRSEPRIPFLSVVEA
GHVRPGETLTDERRRFRATVRPDGQLSVGPAMGSIHKVGALVQGLPACNGWTFWHAERSGKLVCIDDFRTEMRGQAGA
>Mature_397_residues
ASPRTAVVGATARNQVSRTGRIASAPRMGLAPSVQRLPSTLPLDEILIGDCIAAMDRLPAESVDCVFADPPYNLQLGEAG
LTRPDQSVVDAVDDDWDKFSSFSAYDDFTRAWLKAARRVMKPNATLWVIGSYHNIFRVGSALQDLGYWILNDIVWRKANP
MPNFRGKRFTNAHETLIWASRSPQAKYTFHYDALKAGNEDLQMRSDWFIPLCTGEERLKDEAGRKVHPTQKPEALLARTL
LAATNPGDVVLDPFFGTGTTGAVAKRLGRHFIGCERDPTYATAARARIDGIETLSAASLALATPKRSEPRIPFLSVVEAG
HVRPGETLTDERRRFRATVRPDGQLSVGPAMGSIHKVGALVQGLPACNGWTFWHAERSGKLVCIDDFRTEMRGQAGA

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. CcrM-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=282, Percent_Identity=31.5602836879433, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 43727; Mature: 43596

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASPRTAVVGATARNQVSRTGRIASAPRMGLAPSVQRLPSTLPLDEILIGDCIAAMDRLP
CCCCCCEEEECHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCC
AESVDCVFADPPYNLQLGEAGLTRPDQSVVDAVDDDWDKFSSFSAYDDFTRAWLKAARRV
CCCCCEEECCCCCCEEECCCCCCCCCHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHH
MKPNATLWVIGSYHNIFRVGSALQDLGYWILNDIVWRKANPMPNFRGKRFTNAHETLIWA
HCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
SRSPQAKYTFHYDALKAGNEDLQMRSDWFIPLCTGEERLKDEAGRKVHPTQKPEALLART
CCCCCCEEEEEEHHHHCCCCCCEECCCCEEEEECCHHHHHHHCCCCCCCCCCHHHHHHHH
LLAATNPGDVVLDPFFGTGTTGAVAKRLGRHFIGCERDPTYATAARARIDGIETLSAASL
HHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHH
ALATPKRSEPRIPFLSVVEAGHVRPGETLTDERRRFRATVRPDGQLSVGPAMGSIHKVGA
EEECCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHEEECCCCCCEEECCCHHHHHHHHH
LVQGLPACNGWTFWHAERSGKLVCIDDFRTEMRGQAGA
HHHCCCCCCCEEEEEECCCCCEEEEECHHHHHCCCCCC
>Mature Secondary Structure 
ASPRTAVVGATARNQVSRTGRIASAPRMGLAPSVQRLPSTLPLDEILIGDCIAAMDRLP
CCCCCEEEECHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCC
AESVDCVFADPPYNLQLGEAGLTRPDQSVVDAVDDDWDKFSSFSAYDDFTRAWLKAARRV
CCCCCEEECCCCCCEEECCCCCCCCCHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHH
MKPNATLWVIGSYHNIFRVGSALQDLGYWILNDIVWRKANPMPNFRGKRFTNAHETLIWA
HCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
SRSPQAKYTFHYDALKAGNEDLQMRSDWFIPLCTGEERLKDEAGRKVHPTQKPEALLART
CCCCCCEEEEEEHHHHCCCCCCEECCCCEEEEECCHHHHHHHCCCCCCCCCCHHHHHHHH
LLAATNPGDVVLDPFFGTGTTGAVAKRLGRHFIGCERDPTYATAARARIDGIETLSAASL
HHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHH
ALATPKRSEPRIPFLSVVEAGHVRPGETLTDERRRFRATVRPDGQLSVGPAMGSIHKVGA
EEECCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHEEECCCCCCEEECCCHHHHHHHHH
LVQGLPACNGWTFWHAERSGKLVCIDDFRTEMRGQAGA
HHHCCCCCCCEEEEEECCCCCEEEEECHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]