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The map label for this gene is glyA

Identifier: 218531427

GI number: 218531427

Start: 3679135

End: 3680439

Strand: Reverse

Name: glyA

Synonym: Mchl_3495

Alternate gene names: 218531427

Gene position: 3680439-3679135 (Counterclockwise)

Preceding gene: 218531428

Following gene: 218531426

Centisome position: 63.7

GC content: 67.51

Gene sequence:

>1305_bases
ATGAGCGCCGGAACTGCGACCGACACCACCGACCTCGACTCCTTCTTCTCGGCTCATCTTGCCGAGACCGATCCCGAGAT
CGCCAAGGCCATCTCGCAGGAGCTGGGCCGGCAGCAGCACGAGATCGAGCTGATCGCGTCCGAGAACATCGTCTCGCGCG
CCGTGCTCGAAGCGCAGGGCTCGGTCCTGACCAACAAGTACGCCGAGGGCTATCCGGGCCGGCGCTACTACGGCGGCTGC
CAGTTCGTGGACATCGCCGAAGAACTCGCCATCGACCGGGCCAAGCGCCTGTTCGGCTGCGGCTTCGCCAACGTGCAGCC
GAATTCCGGCTCGCAGGCGAACCAGGGCGTGTTCATGGCCCTGATGCAGCCCGGCGACACCTTCCTCGGTCTCGACCTCG
CGGCCGGCGGCCACCTCACCCACGGCGCCCCCCCGAACGTCTCGGGCAAGTGGTTCAAGCCGGTCTCCTACACCGTGCGC
CGCGAAGATCAGCGCATCGACATGGAGCAGGTCGAGCGCCTTGCCCAGGAGCACAAGCCGAAGGTGATCATCGCCGGCGG
CTCGGGCTACCCGCGTCACTGGGACTTCGCGAAGTTCCGTGAGATCGCCGATTCCGTCGGTGCCTACTTCTTCGTCGACA
TGGCCCACTTCGCCGGCCTCGTCGCCGCGGGCCTGCACCCGTCGCCGTTCCCGCACGCGCACGTCGCCACCACGACGACG
CACAAGACCCTGCGCGGCCCGCGCGGCGGCATGATTCTGACGAACGACGAGGCGCTGGCCAAGAAGTTCAACTCGGCGAT
CTTCCCCGGCCTCCAGGGCGGCCCGTTGATGCACGTCATTGCCGCCAAGGCGGTGGCCTTCGGCGAGGCGCTGAAGCCCG
AGTTCAAGATCTACGCCAAGCAGGTGATCGACAACGCCCGCGCGCTGGCCGACACCATCATCTCCGGCGGCTACGACATC
ACCTCGGGCGGCACCGACAACCACCTGATGCTGGTCGACCTTCAGAAGAAGGGCCTGACCGGCAAGGCGGCCGAGGCGGC
GCTCTCCCGGGCCGACATCACCTGCAACAAGAACGGCGTGCCGTTCGACCCGCAGAAGCCGACGATCACCTCCGGCATCC
GTCTCGGCACGCCGGCCAGCACCACTCGCGGCTTCGGCGTCGCCGAGTTCAAGCAGGTCGGCTCGCTGATCGTTCAGGTG
CTCGATGGCATCGCCGAGAAGGGCGATGGCGGCGACGCGGCGGTCGAGGCGGCGGTGAAGGAGAAGGTCCACGCCCTCAC
CGATCGGTTCCCGATCTACGCCTGA

Upstream 100 bases:

>100_bases
ATGCCGCGGCGTGTACGAATAGTGCCTGAAAAACACCCCCGTGAACGCCGACGGGAGAACGGGTGGGTTTGCCCGTAAAC
AACGAAAAGAGGCTACGCTC

Downstream 100 bases:

>100_bases
GCCTGACTAAAAACTCCTCCGGCCCGTGACGCGGGCCGGTGCGGCTGCGGATCTCCCGATCCGCGGCCGCTGTCGTTTTG
GGGCGGCCTCAAAGTTAATC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSAGTATDTTDLDSFFSAHLAETDPEIAKAISQELGRQQHEIELIASENIVSRAVLEAQGSVLTNKYAEGYPGRRYYGGC
QFVDIAEELAIDRAKRLFGCGFANVQPNSGSQANQGVFMALMQPGDTFLGLDLAAGGHLTHGAPPNVSGKWFKPVSYTVR
REDQRIDMEQVERLAQEHKPKVIIAGGSGYPRHWDFAKFREIADSVGAYFFVDMAHFAGLVAAGLHPSPFPHAHVATTTT
HKTLRGPRGGMILTNDEALAKKFNSAIFPGLQGGPLMHVIAAKAVAFGEALKPEFKIYAKQVIDNARALADTIISGGYDI
TSGGTDNHLMLVDLQKKGLTGKAAEAALSRADITCNKNGVPFDPQKPTITSGIRLGTPASTTRGFGVAEFKQVGSLIVQV
LDGIAEKGDGGDAAVEAAVKEKVHALTDRFPIYA

Sequences:

>Translated_434_residues
MSAGTATDTTDLDSFFSAHLAETDPEIAKAISQELGRQQHEIELIASENIVSRAVLEAQGSVLTNKYAEGYPGRRYYGGC
QFVDIAEELAIDRAKRLFGCGFANVQPNSGSQANQGVFMALMQPGDTFLGLDLAAGGHLTHGAPPNVSGKWFKPVSYTVR
REDQRIDMEQVERLAQEHKPKVIIAGGSGYPRHWDFAKFREIADSVGAYFFVDMAHFAGLVAAGLHPSPFPHAHVATTTT
HKTLRGPRGGMILTNDEALAKKFNSAIFPGLQGGPLMHVIAAKAVAFGEALKPEFKIYAKQVIDNARALADTIISGGYDI
TSGGTDNHLMLVDLQKKGLTGKAAEAALSRADITCNKNGVPFDPQKPTITSGIRLGTPASTTRGFGVAEFKQVGSLIVQV
LDGIAEKGDGGDAAVEAAVKEKVHALTDRFPIYA
>Mature_433_residues
SAGTATDTTDLDSFFSAHLAETDPEIAKAISQELGRQQHEIELIASENIVSRAVLEAQGSVLTNKYAEGYPGRRYYGGCQ
FVDIAEELAIDRAKRLFGCGFANVQPNSGSQANQGVFMALMQPGDTFLGLDLAAGGHLTHGAPPNVSGKWFKPVSYTVRR
EDQRIDMEQVERLAQEHKPKVIIAGGSGYPRHWDFAKFREIADSVGAYFFVDMAHFAGLVAAGLHPSPFPHAHVATTTTH
KTLRGPRGGMILTNDEALAKKFNSAIFPGLQGGPLMHVIAAKAVAFGEALKPEFKIYAKQVIDNARALADTIISGGYDIT
SGGTDNHLMLVDLQKKGLTGKAAEAALSRADITCNKNGVPFDPQKPTITSGIRLGTPASTTRGFGVAEFKQVGSLIVQVL
DGIAEKGDGGDAAVEAAVKEKVHALTDRFPIYA

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=402, Percent_Identity=47.7611940298507, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI261862350, Length=402, Percent_Identity=47.7611940298507, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI261862348, Length=402, Percent_Identity=47.7611940298507, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI19923315, Length=402, Percent_Identity=47.7611940298507, Blast_Score=342, Evalue=5e-94,
Organism=Homo sapiens, GI22547186, Length=464, Percent_Identity=45.0431034482759, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI261862346, Length=397, Percent_Identity=47.6070528967254, Blast_Score=333, Evalue=1e-91,
Organism=Homo sapiens, GI22547189, Length=447, Percent_Identity=42.7293064876958, Blast_Score=303, Evalue=2e-82,
Organism=Escherichia coli, GI1788902, Length=418, Percent_Identity=60.0478468899521, Blast_Score=475, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI25144732, Length=412, Percent_Identity=46.6019417475728, Blast_Score=344, Evalue=5e-95,
Organism=Caenorhabditis elegans, GI25144729, Length=412, Percent_Identity=46.6019417475728, Blast_Score=344, Evalue=6e-95,
Organism=Saccharomyces cerevisiae, GI6319739, Length=412, Percent_Identity=45.3883495145631, Blast_Score=350, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6323087, Length=411, Percent_Identity=43.7956204379562, Blast_Score=327, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24640005, Length=400, Percent_Identity=48.75, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI221329721, Length=400, Percent_Identity=48.75, Blast_Score=358, Evalue=3e-99,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_METC4 (B7KVA7)

Other databases:

- EMBL:   CP001298
- RefSeq:   YP_002422243.1
- ProteinModelPortal:   B7KVA7
- SMR:   B7KVA7
- GeneID:   7114253
- GenomeReviews:   CP001298_GR
- KEGG:   mch:Mchl_3495
- HOGENOM:   HBG301263
- OMA:   IDMEQVA
- ProtClustDB:   PRK00011
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46334; Mature: 46203

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGTATDTTDLDSFFSAHLAETDPEIAKAISQELGRQQHEIELIASENIVSRAVLEAQG
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHEEEEEECCCHHHHHHHHHCC
SVLTNKYAEGYPGRRYYGGCQFVDIAEELAIDRAKRLFGCGFANVQPNSGSQANQGVFMA
CHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
LMQPGDTFLGLDLAAGGHLTHGAPPNVSGKWFKPVSYTVRREDQRIDMEQVERLAQEHKP
EECCCCCEEEEEEECCCCCCCCCCCCCCCCEECCCHHEECCCCCCCCHHHHHHHHHHCCC
KVIIAGGSGYPRHWDFAKFREIADSVGAYFFVDMAHFAGLVAAGLHPSPFPHAHVATTTT
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCC
HKTLRGPRGGMILTNDEALAKKFNSAIFPGLQGGPLMHVIAAKAVAFGEALKPEFKIYAK
HHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
QVIDNARALADTIISGGYDITSGGTDNHLMLVDLQKKGLTGKAAEAALSRADITCNKNGV
HHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEECCCCC
PFDPQKPTITSGIRLGTPASTTRGFGVAEFKQVGSLIVQVLDGIAEKGDGGDAAVEAAVK
CCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EKVHALTDRFPIYA
HHHHHHHHCCCCCC
>Mature Secondary Structure 
SAGTATDTTDLDSFFSAHLAETDPEIAKAISQELGRQQHEIELIASENIVSRAVLEAQG
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHEEEEEECCCHHHHHHHHHCC
SVLTNKYAEGYPGRRYYGGCQFVDIAEELAIDRAKRLFGCGFANVQPNSGSQANQGVFMA
CHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
LMQPGDTFLGLDLAAGGHLTHGAPPNVSGKWFKPVSYTVRREDQRIDMEQVERLAQEHKP
EECCCCCEEEEEEECCCCCCCCCCCCCCCCEECCCHHEECCCCCCCCHHHHHHHHHHCCC
KVIIAGGSGYPRHWDFAKFREIADSVGAYFFVDMAHFAGLVAAGLHPSPFPHAHVATTTT
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCC
HKTLRGPRGGMILTNDEALAKKFNSAIFPGLQGGPLMHVIAAKAVAFGEALKPEFKIYAK
HHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
QVIDNARALADTIISGGYDITSGGTDNHLMLVDLQKKGLTGKAAEAALSRADITCNKNGV
HHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEECCCCC
PFDPQKPTITSGIRLGTPASTTRGFGVAEFKQVGSLIVQVLDGIAEKGDGGDAAVEAAVK
CCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EKVHALTDRFPIYA
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA