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The map label for this gene is mdeA [H]

Identifier: 218531405

GI number: 218531405

Start: 3657911

End: 3659218

Strand: Reverse

Name: mdeA [H]

Synonym: Mchl_3473

Alternate gene names: 218531405

Gene position: 3659218-3657911 (Counterclockwise)

Preceding gene: 218531406

Following gene: 218531404

Centisome position: 63.33

GC content: 67.97

Gene sequence:

>1308_bases
ATGACCGCCGAGCCAAAAACCCTCCGCTTCGCCACGCAGGCGGTCCATGCGGGCGCTGCCCCTGATCCCGCCACCGGTGC
TCGGGTGCAGCCGATCTACTTCACCAACGGCTTCGTCTTCGACTCGACAGAGCAGGCCGCCGACATCTTCGCCATGCGCA
AGACCGGCTTCTCCTACTCGCGCGGCTCGAACCCGACGGTCGCCGCGCTGGAGCGCCGGGTCGCTGCATTGGAGGGCGCC
AAGGCGGCGGTCGCCGTCTCGTCCGGGCAGTCGGCGGTTCTGCTGGTGATGATGACGCTGATGCAGACGGGCGACGCCTA
CGTCGCCTCGCCGCGCCTGTTCGGCGGCTCGCTCGGCCTGATGCGCCGCCTCGAAGGCCGCTACGACCTGACGCCGCAAT
TCGCCGCGGACCTGACGCCGGAGGCGTTCGAAGCCGCGATCACGCCGCAGACCAAGGCGATCATCTGTGAGTCGATCGTC
AATCCCTGCGCCACGGTGATGGATATCGAGGGCATCGCCGCGGTCGCTAAGCGCCACGGCCTGCCGCTCGTCATCGACAA
CACCCTGGCCTCGCCCGCGCTGATCCGCCCGATCGAGTACGGCGCCGACATCATCGTCCACTCGACGTCGAAGTTCCTCG
GCGGCTCGGGCCAAGTGATCGGCGGCATGATCTGCGATGCCGGCACCTTCGATTGGAAGGCGCAGGGCTCGCGTTACAAC
CTCATCAACGATCCCTGGCCGGACTATGACGGGCTGATCGTCAGCGACCGCTTCCCCGAGATCAGCTTCGCCGTGGCCTG
TCGCCTGTTCGGCCTGCGCGACCTCGGCCCCGGCCTCTCGCCGATGAACGCCTTCCTGACGCTCACCGGCATCGAGACGC
TGCCCCTGCGGATGGAGCGCCACTGCGCCAACGCCAAGGCGGTTGCGGCCTACCTCAAGGACCATCCGGCGGTGGATTGG
GTGAGCTACCCGGCGCTTCCCGGCCAGAAGGGTGAGGCTTTGGCCAACCGCTACGTGCCGCAGGGCCCGGGCTCGATCTT
CACCTTCGCCCTGAAGGGCGGCGAGCCGGCGGCCCTGGCGTTCATCGCGAACCTCGAACTGGTCTCGCACCTCGTGAACA
TCGGCGAGATCAAGTCACTGGTGATCCACCCGGCCACCACGACCCATCGCCAGCTCCGGCCCGAGGAGCGCGCGGCGGCC
TGCGTCGGCCCCGAGACCGTGCGTCTCTCGATCGGCCTGGAGGATCCCGAAGATCTCATCGCCGATATCGAGCAAGCGCT
GGCCAAGGTCGGCGCACAGATCGGCTGA

Upstream 100 bases:

>100_bases
GCCAATCGCGGGTTTCGCCGCGGTCCGTTTCGCGGCAGAAGGCGGGTGAGAGTGGCGCGCTGCCCCGCGCGCCGACAGAC
CGATCAGTTCAGGTCCCGCC

Downstream 100 bases:

>100_bases
GGCCGCGATGGGACGGCGCGGGCGCATCGCTGCGGTGATCGGCGGCCTCGCCGTCCTCGGCGCCGGTGCCGTCGTCCTCG
CCGACGTCTCGCAAGTCGGC

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTAEPKTLRFATQAVHAGAAPDPATGARVQPIYFTNGFVFDSTEQAADIFAMRKTGFSYSRGSNPTVAALERRVAALEGA
KAAVAVSSGQSAVLLVMMTLMQTGDAYVASPRLFGGSLGLMRRLEGRYDLTPQFAADLTPEAFEAAITPQTKAIICESIV
NPCATVMDIEGIAAVAKRHGLPLVIDNTLASPALIRPIEYGADIIVHSTSKFLGGSGQVIGGMICDAGTFDWKAQGSRYN
LINDPWPDYDGLIVSDRFPEISFAVACRLFGLRDLGPGLSPMNAFLTLTGIETLPLRMERHCANAKAVAAYLKDHPAVDW
VSYPALPGQKGEALANRYVPQGPGSIFTFALKGGEPAALAFIANLELVSHLVNIGEIKSLVIHPATTTHRQLRPEERAAA
CVGPETVRLSIGLEDPEDLIADIEQALAKVGAQIG

Sequences:

>Translated_435_residues
MTAEPKTLRFATQAVHAGAAPDPATGARVQPIYFTNGFVFDSTEQAADIFAMRKTGFSYSRGSNPTVAALERRVAALEGA
KAAVAVSSGQSAVLLVMMTLMQTGDAYVASPRLFGGSLGLMRRLEGRYDLTPQFAADLTPEAFEAAITPQTKAIICESIV
NPCATVMDIEGIAAVAKRHGLPLVIDNTLASPALIRPIEYGADIIVHSTSKFLGGSGQVIGGMICDAGTFDWKAQGSRYN
LINDPWPDYDGLIVSDRFPEISFAVACRLFGLRDLGPGLSPMNAFLTLTGIETLPLRMERHCANAKAVAAYLKDHPAVDW
VSYPALPGQKGEALANRYVPQGPGSIFTFALKGGEPAALAFIANLELVSHLVNIGEIKSLVIHPATTTHRQLRPEERAAA
CVGPETVRLSIGLEDPEDLIADIEQALAKVGAQIG
>Mature_434_residues
TAEPKTLRFATQAVHAGAAPDPATGARVQPIYFTNGFVFDSTEQAADIFAMRKTGFSYSRGSNPTVAALERRVAALEGAK
AAVAVSSGQSAVLLVMMTLMQTGDAYVASPRLFGGSLGLMRRLEGRYDLTPQFAADLTPEAFEAAITPQTKAIICESIVN
PCATVMDIEGIAAVAKRHGLPLVIDNTLASPALIRPIEYGADIIVHSTSKFLGGSGQVIGGMICDAGTFDWKAQGSRYNL
INDPWPDYDGLIVSDRFPEISFAVACRLFGLRDLGPGLSPMNAFLTLTGIETLPLRMERHCANAKAVAAYLKDHPAVDWV
SYPALPGQKGEALANRYVPQGPGSIFTFALKGGEPAALAFIANLELVSHLVNIGEIKSLVIHPATTTHRQLRPEERAAAC
VGPETVRLSIGLEDPEDLIADIEQALAKVGAQIG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=425, Percent_Identity=33.4117647058824, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI299473759, Length=425, Percent_Identity=31.7647058823529, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI25453487, Length=424, Percent_Identity=29.2452830188679, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1790375, Length=427, Percent_Identity=32.3185011709602, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789383, Length=432, Percent_Identity=25.462962962963, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71980708, Length=432, Percent_Identity=28.7037037037037, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17560056, Length=424, Percent_Identity=31.1320754716981, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17538055, Length=424, Percent_Identity=31.1320754716981, Blast_Score=164, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6323333, Length=437, Percent_Identity=39.5881006864989, Blast_Score=290, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6319307, Length=428, Percent_Identity=30.607476635514, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321254, Length=449, Percent_Identity=24.7216035634744, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321170, Length=345, Percent_Identity=24.0579710144928, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19922554, Length=424, Percent_Identity=30.8962264150943, Blast_Score=189, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46186; Mature: 46055

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEPKTLRFATQAVHAGAAPDPATGARVQPIYFTNGFVFDSTEQAADIFAMRKTGFSYS
CCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCEEECCCHHHHHHHHHHHCCCCCC
RGSNPTVAALERRVAALEGAKAAVAVSSGQSAVLLVMMTLMQTGDAYVASPRLFGGSLGL
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEECCCCCCCHHHH
MRRLEGRYDLTPQFAADLTPEAFEAAITPQTKAIICESIVNPCATVMDIEGIAAVAKRHG
HHHHCCCCCCCCHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPLVIDNTLASPALIRPIEYGADIIVHSTSKFLGGSGQVIGGMICDAGTFDWKAQGSRYN
CCEEECCCCCCCHHCCHHHCCCEEEEECCHHHCCCCCCEEEEEEECCCCCCCCCCCCEEC
LINDPWPDYDGLIVSDRFPEISFAVACRLFGLRDLGPGLSPMNAFLTLTGIETLPLRMER
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHEEEEECCCCCCHHHHH
HCANAKAVAAYLKDHPAVDWVSYPALPGQKGEALANRYVPQGPGSIFTFALKGGEPAALA
HHCCHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHCCCCCCCCCEEEEEECCCCCEEEH
FIANLELVSHLVNIGEIKSLVIHPATTTHRQLRPEERAAACVGPETVRLSIGLEDPEDLI
HHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCCCHHHHHHHCCCCEEEEEECCCCHHHHH
ADIEQALAKVGAQIG
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
TAEPKTLRFATQAVHAGAAPDPATGARVQPIYFTNGFVFDSTEQAADIFAMRKTGFSYS
CCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCEEECCCHHHHHHHHHHHCCCCCC
RGSNPTVAALERRVAALEGAKAAVAVSSGQSAVLLVMMTLMQTGDAYVASPRLFGGSLGL
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEECCCCCCCHHHH
MRRLEGRYDLTPQFAADLTPEAFEAAITPQTKAIICESIVNPCATVMDIEGIAAVAKRHG
HHHHCCCCCCCCHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPLVIDNTLASPALIRPIEYGADIIVHSTSKFLGGSGQVIGGMICDAGTFDWKAQGSRYN
CCEEECCCCCCCHHCCHHHCCCEEEEECCHHHCCCCCCEEEEEEECCCCCCCCCCCCEEC
LINDPWPDYDGLIVSDRFPEISFAVACRLFGLRDLGPGLSPMNAFLTLTGIETLPLRMER
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHEEEEECCCCCCHHHHH
HCANAKAVAAYLKDHPAVDWVSYPALPGQKGEALANRYVPQGPGSIFTFALKGGEPAALA
HHCCHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHCCCCCCCCCEEEEEECCCCCEEEH
FIANLELVSHLVNIGEIKSLVIHPATTTHRQLRPEERAAACVGPETVRLSIGLEDPEDLI
HHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCCCHHHHHHHCCCCEEEEEECCCCHHHHH
ADIEQALAKVGAQIG
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]