BacMap

Definition	Methylobacterium chloromethanicum CM4, complete genome.
Accession	NC_011757
Length	5,777,908

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The map label for this gene is yvaG [H]

Identifier: 218531381

GI number: 218531381

Start: 3636356

End: 3637147

Strand: Reverse

Name: yvaG [H]

Synonym: Mchl_3449

Alternate gene names: 218531381

Gene position: 3637147-3636356 (Counterclockwise)

Preceding gene: 218531382

Following gene: 218531379

Centisome position: 62.95

GC content: 70.33

Gene sequence:

>792_bases
ATGGATATCGACCTGAAGGGGCGCAGTGCCCTCGTCACCGGTTCCACCGGCGGCATCGGCTACGCGGTGGCCCGCGAACT
CGCCCGGCTCGGCGCCTACGTCGCCGTCAACGGCCGCACCGGTGAGCGGGTGGAGGCGGCCATCGCGCGCCTGCGCGGCG
AGGTGCCGGGGGCCGAGTTCATCGCCGGCATCGGCGATGTCGGCACCGAGGAGGGGGCGGGCGAGCTCCTCGCCGCCCTG
CCGCGGGTCGACATCCTGATCAACAATGCCGGCATCTTCGAGCCGAAGCCATTCTTCGAGATCGCGGATGCCGAGTGGCG
CCGCTTCTTCGAGATGAACGTGATGAGCGGCATCCGCCTCTCCCGCGCCTACGGTCCCGGCATGGTCGAGCGCGGCTGGG
GACGGATCGTCTTCATCGCCAGCGAGTCGGGCGTCAACATCCCGCCGGAGATGGTCCATTACGGCGTGACGAAAACGGCG
CAGCTCGCCGTCTCCCGCGGTCTCGCCGAGACGGTGGGCGGCAGCGGCGTCACCGTCAACAGCGTGCTCCCCGGTCCGAC
CATGACCGAGGGCGTCGCGGATTTTCTCGAATCGATGGGCGGGGGCGAAGGCGATCTGGAGGCCAAGGGCCGCGCCTTCA
TCGCCGAGAACCGGCCGACCTCGCTCCTGAAGCGCCTCGCGCAGCCGGAGGAAGTGGCCAACATGGTCGCCTATCTCTGC
ACGCCCGCCGCGAGCGCCACCACGGGGGCGGCCCTGCGGGTCGATGGCGGCGTGCTGCGCGGCATCATCTGA

Upstream 100 bases:

>100_bases
CGGCGGACGGGACCCTGACCGCGCATCTTCGCTCAGCACCATGGCCCGGCTCGTGCGTTGACGCCGGGACAGGGATGGCC
GCGGCCAAGGGAGGCGGATG

Downstream 100 bases:

>100_bases
AACTCACCGCTCCCGAGCCCACCGGACGTACGGGGCCGGCTTGACCGGCACCGTACTCCGAGGGACGGCCCGCGAACGGT
TCCTTCAAATCAGGACTTGA

Product: short-chain dehydrogenase/reductase SDR

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MDIDLKGRSALVTGSTGGIGYAVARELARLGAYVAVNGRTGERVEAAIARLRGEVPGAEFIAGIGDVGTEEGAGELLAAL
PRVDILINNAGIFEPKPFFEIADAEWRRFFEMNVMSGIRLSRAYGPGMVERGWGRIVFIASESGVNIPPEMVHYGVTKTA
QLAVSRGLAETVGGSGVTVNSVLPGPTMTEGVADFLESMGGGEGDLEAKGRAFIAENRPTSLLKRLAQPEEVANMVAYLC
TPAASATTGAALRVDGGVLRGII

Sequences:

>Translated_263_residues
MDIDLKGRSALVTGSTGGIGYAVARELARLGAYVAVNGRTGERVEAAIARLRGEVPGAEFIAGIGDVGTEEGAGELLAAL
PRVDILINNAGIFEPKPFFEIADAEWRRFFEMNVMSGIRLSRAYGPGMVERGWGRIVFIASESGVNIPPEMVHYGVTKTA
QLAVSRGLAETVGGSGVTVNSVLPGPTMTEGVADFLESMGGGEGDLEAKGRAFIAENRPTSLLKRLAQPEEVANMVAYLC
TPAASATTGAALRVDGGVLRGII
>Mature_263_residues
MDIDLKGRSALVTGSTGGIGYAVARELARLGAYVAVNGRTGERVEAAIARLRGEVPGAEFIAGIGDVGTEEGAGELLAAL
PRVDILINNAGIFEPKPFFEIADAEWRRFFEMNVMSGIRLSRAYGPGMVERGWGRIVFIASESGVNIPPEMVHYGVTKTA
QLAVSRGLAETVGGSGVTVNSVLPGPTMTEGVADFLESMGGGEGDLEAKGRAFIAENRPTSLLKRLAQPEEVANMVAYLC
TPAASATTGAALRVDGGVLRGII

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=261, Percent_Identity=31.8007662835249, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI5031737, Length=264, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI19923817, Length=261, Percent_Identity=30.2681992337165, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI126723750, Length=262, Percent_Identity=30.9160305343511, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI7705925, Length=258, Percent_Identity=31.0077519379845, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI126723191, Length=186, Percent_Identity=32.7956989247312, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI33667109, Length=198, Percent_Identity=35.8585858585859, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI10190704, Length=258, Percent_Identity=30.6201550387597, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI7705855, Length=184, Percent_Identity=31.5217391304348, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI7706318, Length=192, Percent_Identity=27.6041666666667, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI109715829, Length=248, Percent_Identity=31.0483870967742, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4504505, Length=259, Percent_Identity=26.6409266409266, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI226371731, Length=207, Percent_Identity=29.4685990338164, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI142976729, Length=186, Percent_Identity=26.8817204301075, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI10190746, Length=217, Percent_Identity=29.4930875576037, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI146231950, Length=208, Percent_Identity=31.7307692307692, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI66933014, Length=193, Percent_Identity=24.8704663212435, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI7657478, Length=203, Percent_Identity=29.064039408867, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787905, Length=263, Percent_Identity=32.319391634981, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1787335, Length=260, Percent_Identity=33.4615384615385, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1790717, Length=265, Percent_Identity=28.3018867924528, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082100, Length=268, Percent_Identity=29.4776119402985, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788459, Length=260, Percent_Identity=30, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1786812, Length=257, Percent_Identity=29.5719844357977, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI2367365, Length=259, Percent_Identity=30.8880308880309, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789378, Length=260, Percent_Identity=32.3076923076923, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787820, Length=182, Percent_Identity=33.5164835164835, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI87082160, Length=275, Percent_Identity=28.3636363636364, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789208, Length=274, Percent_Identity=25.9124087591241, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI2367175, Length=257, Percent_Identity=30.3501945525292, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17561402, Length=263, Percent_Identity=32.6996197718631, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17560676, Length=260, Percent_Identity=31.5384615384615, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI193204405, Length=264, Percent_Identity=32.9545454545455, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17531453, Length=271, Percent_Identity=31.3653136531365, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71994604, Length=263, Percent_Identity=31.5589353612167, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17562906, Length=264, Percent_Identity=31.0606060606061, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71994600, Length=260, Percent_Identity=28.4615384615385, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI115534694, Length=262, Percent_Identity=32.4427480916031, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17562908, Length=264, Percent_Identity=31.0606060606061, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17562910, Length=261, Percent_Identity=29.5019157088123, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17562904, Length=264, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17562990, Length=262, Percent_Identity=28.6259541984733, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17559104, Length=261, Percent_Identity=30.2681992337165, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17568967, Length=204, Percent_Identity=30.3921568627451, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17560150, Length=269, Percent_Identity=27.8810408921933, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17560332, Length=269, Percent_Identity=31.9702602230483, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17560220, Length=271, Percent_Identity=28.7822878228782, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI25147288, Length=259, Percent_Identity=27.027027027027, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI72000259, Length=265, Percent_Identity=28.3018867924528, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17544670, Length=266, Percent_Identity=28.1954887218045, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17565030, Length=259, Percent_Identity=30.8880308880309, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538480, Length=271, Percent_Identity=26.9372693726937, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17564282, Length=187, Percent_Identity=28.8770053475936, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=269, Percent_Identity=26.7657992565056, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17551412, Length=265, Percent_Identity=26.7924528301887, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17538486, Length=261, Percent_Identity=27.2030651340996, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=249, Percent_Identity=28.5140562248996, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324126, Length=261, Percent_Identity=24.5210727969349, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644339, Length=259, Percent_Identity=30.1158301158301, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21357041, Length=256, Percent_Identity=30.859375, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28571526, Length=257, Percent_Identity=31.5175097276265, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI23397609, Length=257, Percent_Identity=32.295719844358, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355319, Length=263, Percent_Identity=28.1368821292776, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24643142, Length=257, Percent_Identity=27.2373540856031, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24639444, Length=257, Percent_Identity=27.6264591439689, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24644337, Length=265, Percent_Identity=30.9433962264151, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21358495, Length=234, Percent_Identity=28.2051282051282, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24641232, Length=241, Percent_Identity=28.2157676348548, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI18859837, Length=252, Percent_Identity=27.7777777777778, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24640492, Length=164, Percent_Identity=30.4878048780488, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24640442, Length=198, Percent_Identity=29.2929292929293, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 27422; Mature: 27422

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIDLKGRSALVTGSTGGIGYAVARELARLGAYVAVNGRTGERVEAAIARLRGEVPGAEF
CCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCHHH
IAGIGDVGTEEGAGELLAALPRVDILINNAGIFEPKPFFEIADAEWRRFFEMNVMSGIRL
HHCCCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCEECCHHHHHHHHHHHHHCCEE
SRAYGPGMVERGWGRIVFIASESGVNIPPEMVHYGVTKTAQLAVSRGLAETVGGSGVTVN
HHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEE
SVLPGPTMTEGVADFLESMGGGEGDLEAKGRAFIAENRPTSLLKRLAQPEEVANMVAYLC
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHCCHHHHHHHHHHHH
TPAASATTGAALRVDGGVLRGII
CCCCCCCCCCEEEECCHHHHCCC
>Mature Secondary Structure
MDIDLKGRSALVTGSTGGIGYAVARELARLGAYVAVNGRTGERVEAAIARLRGEVPGAEF
CCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCHHH
IAGIGDVGTEEGAGELLAALPRVDILINNAGIFEPKPFFEIADAEWRRFFEMNVMSGIRL
HHCCCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCEECCHHHHHHHHHHHHHCCEE
SRAYGPGMVERGWGRIVFIASESGVNIPPEMVHYGVTKTAQLAVSRGLAETVGGSGVTVN
HHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEE
SVLPGPTMTEGVADFLESMGGGEGDLEAKGRAFIAENRPTSLLKRLAQPEEVANMVAYLC
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHCCHHHHHHHHHHHH
TPAASATTGAALRVDGGVLRGII
CCCCCCCCCCEEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]