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The map label for this gene is glyS

Identifier: 218531348

GI number: 218531348

Start: 3603070

End: 3605160

Strand: Reverse

Name: glyS

Synonym: Mchl_3416

Alternate gene names: 218531348

Gene position: 3605160-3603070 (Counterclockwise)

Preceding gene: 218531349

Following gene: 218531347

Centisome position: 62.4

GC content: 69.97

Gene sequence:

>2091_bases
ATGCCTGACCTTCTGCTCGAACTCCGCTCCGAAGAGATCCCCGCGCGCATGCAGCGGCGTGCGGCCGAGGATCTGAAGAA
GCTCGTCACCGACGCGCTGGTCGAGCGCGGTTTCCTCTACGAGGGCGCCAAGGCCTTCGCGACGCCGCGGCGGCTGGCGC
TGCACGTCGCCGGGCTGCCCGCCCGCGGCGAGGCGGTGCGCGAGGAGCGGCGCGGCCCCCGCGTCGGCGCGCCGGAAGCC
GCCGTGCAGGGCTTCCTGAAGAGCGCGGGGCTCGCGAGCCTCGATCAGGCCACTACGGTGACGGATCCGAAGAAGGGCGA
GTTCTACCTCGCAGTGATCGAGCGGCCCGGCCGCGAGACGCTGGACGTGCTGGCCGAGATCCTGCCGGGCATCATCAAGA
GCTTCCCCTGGCCGAAATCGATGCGCTGGGGCGCGGCCTCGGCCCAACCCGGCTCCCTGCGTTGGGTGCGCCCGCTGCAA
TCGATTGTCGCCACCTTCGGCCCCGAGACCGAGACGCCGGAAGTCGTGCCGTTCTCGGTCGATGGCATCACCGCGGGCAT
GGTCACGAGCGGCCATCGCTTCCTCGCGCCCGAACCCTTCGAGGTGCGCCGTTTCGACGATTACGTGCAGGCGCTGGAGC
GCGCCGATGTGATCCTCGATGCCGACCGGCGCAAGGACATCATCCTACACGACGCGAAGGATCTCGCCTTCGCCCGCGGC
CTCGACCTCGTGGAGGACGAGGGCCTGCTGGAGGAGGTGGCCGGGCTGGTGGAGCGGCCCGTGGTGCTGATGGGCTCGTT
CGAGGAGCGTTTCCTGGAGATCCCGGCCGAGGCGATCCGGGCGACGATCCGCGCCAACCAGAAGTGCTTCGTGCTGCGCA
AATCCGGCTCGGACGAACTGGCGCCGGCCTTCGTCCTGGTCTCGAACCTCATCGCCTCCGATGGTGGCGCGGCGATCACC
GCCGGCAACGAGCGCGTGGTGCGGGCCCGCCTCTCGGACGCACGCTTCTTCTGGGAGACCGACAAAGCGACGAAGCTGGA
GGACCGCCTCCCCAAGCTCGATTCGATCGTCTTCCACGAGAAGCTCGGCACGCAAGGCGAGCGGGTCGCCCGCATCGCCG
CGCTCGCAGCGGAAATCGCACCGCTGGTGGCGGCCGACCCGGCGCTCGCCGAGCGCGCGGCCCGGCTTGCCAAGGCCGAT
CTCGTCACCGAGATGGTCGGCGAGTTTCCGGAATTGCAGGGCCTGATGGGCCGCAAGTATGCGGCGCTGCAGGGCGAGCA
CGACAGCGTCGCCGCGGCCATCGAGGAGCACTACAAGCCGGTGGGCCCGAGCGACCGCGTGCCGACCGATCCGGTCTCCA
TCGCCGTGGCGCTCGCCGACAAGCTCGATACGCTGGTTGGGTTCTGGGCGATCGACGAGAAGCCGACGGGGAGCAAGGAC
CCGTATGCACTACGTCGGGCGGCTTTGGGCGTGATCCGCGCCGTGATCGAACGGTCGCTGCGGCTGTCGCTGGCCTCCCT
GCTGAAAGGCCGCTTCGTGAGCGGAAGCGATGAGCGCACCGCGGATCTCCTCGCCTTCTTCGCCGATCGTCTCAAGGTCT
ACCTGCGCGACCAGGGCGCTCGTCACGACCTGATCGACGCGGTGTTCGCCCTTCCCGGCCAGGACGATCTTTTGATGGTC
GTCCGCCGGGTCGAGGCGCTGGGCGCGTTCCTCGCCACCGACGACGGAAAAAACCTGCTCGCCGGCTACAAGCGCGCGGC
CAACATCCTGCGCATCGAGGAGAAGAAGGACGGCCGCGCCTACGACGAGGCCCCCGATGCGGCGCTGGCCGCTTCCGGCC
AGCCGGAGGAGCGGGCGCTCGCCGAGGCCCTGGCAGCGGCCCGCCAAGAGGCGTCCGCGGCGGTGGCGGCCGAGGATTTC
GCCGGGGCGATGCGGGCGCTCTCACGGCTGCGGGCGCCGGTGGACGCCTTCTTCGAAAAGGTCACCGTCAACGCCGACGA
CCCGGCCCTGCGCAAGAACCGACTCCTGCTCCTCAACGCCCTGCGCGCGGCCACCCGCGAAGTGGCCGACTTCTCCCGCA
TCGAGGGCTGA

Upstream 100 bases:

>100_bases
ACTGCTTCGCCGACGCGCGCGATGACGATGGGGCGGCGGTGACAGATGGGGGCCATCTCCCGTCTCGACGCGGCCCGAGC
GGGCGGCTATTCCCGCGCGC

Downstream 100 bases:

>100_bases
CGGGGCGGGCCGGTGCTCCGGCCCGGCTTCCCTGGCTCGGCGGGGCGATCCGGGCTTCGCGGCGGCGTTCGCGCGTGAGC
GTAACATTTCCGCCGCAAGC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 696; Mature: 695

Protein sequence:

>696_residues
MPDLLLELRSEEIPARMQRRAAEDLKKLVTDALVERGFLYEGAKAFATPRRLALHVAGLPARGEAVREERRGPRVGAPEA
AVQGFLKSAGLASLDQATTVTDPKKGEFYLAVIERPGRETLDVLAEILPGIIKSFPWPKSMRWGAASAQPGSLRWVRPLQ
SIVATFGPETETPEVVPFSVDGITAGMVTSGHRFLAPEPFEVRRFDDYVQALERADVILDADRRKDIILHDAKDLAFARG
LDLVEDEGLLEEVAGLVERPVVLMGSFEERFLEIPAEAIRATIRANQKCFVLRKSGSDELAPAFVLVSNLIASDGGAAIT
AGNERVVRARLSDARFFWETDKATKLEDRLPKLDSIVFHEKLGTQGERVARIAALAAEIAPLVAADPALAERAARLAKAD
LVTEMVGEFPELQGLMGRKYAALQGEHDSVAAAIEEHYKPVGPSDRVPTDPVSIAVALADKLDTLVGFWAIDEKPTGSKD
PYALRRAALGVIRAVIERSLRLSLASLLKGRFVSGSDERTADLLAFFADRLKVYLRDQGARHDLIDAVFALPGQDDLLMV
VRRVEALGAFLATDDGKNLLAGYKRAANILRIEEKKDGRAYDEAPDAALAASGQPEERALAEALAAARQEASAAVAAEDF
AGAMRALSRLRAPVDAFFEKVTVNADDPALRKNRLLLLNALRAATREVADFSRIEG

Sequences:

>Translated_696_residues
MPDLLLELRSEEIPARMQRRAAEDLKKLVTDALVERGFLYEGAKAFATPRRLALHVAGLPARGEAVREERRGPRVGAPEA
AVQGFLKSAGLASLDQATTVTDPKKGEFYLAVIERPGRETLDVLAEILPGIIKSFPWPKSMRWGAASAQPGSLRWVRPLQ
SIVATFGPETETPEVVPFSVDGITAGMVTSGHRFLAPEPFEVRRFDDYVQALERADVILDADRRKDIILHDAKDLAFARG
LDLVEDEGLLEEVAGLVERPVVLMGSFEERFLEIPAEAIRATIRANQKCFVLRKSGSDELAPAFVLVSNLIASDGGAAIT
AGNERVVRARLSDARFFWETDKATKLEDRLPKLDSIVFHEKLGTQGERVARIAALAAEIAPLVAADPALAERAARLAKAD
LVTEMVGEFPELQGLMGRKYAALQGEHDSVAAAIEEHYKPVGPSDRVPTDPVSIAVALADKLDTLVGFWAIDEKPTGSKD
PYALRRAALGVIRAVIERSLRLSLASLLKGRFVSGSDERTADLLAFFADRLKVYLRDQGARHDLIDAVFALPGQDDLLMV
VRRVEALGAFLATDDGKNLLAGYKRAANILRIEEKKDGRAYDEAPDAALAASGQPEERALAEALAAARQEASAAVAAEDF
AGAMRALSRLRAPVDAFFEKVTVNADDPALRKNRLLLLNALRAATREVADFSRIEG
>Mature_695_residues
PDLLLELRSEEIPARMQRRAAEDLKKLVTDALVERGFLYEGAKAFATPRRLALHVAGLPARGEAVREERRGPRVGAPEAA
VQGFLKSAGLASLDQATTVTDPKKGEFYLAVIERPGRETLDVLAEILPGIIKSFPWPKSMRWGAASAQPGSLRWVRPLQS
IVATFGPETETPEVVPFSVDGITAGMVTSGHRFLAPEPFEVRRFDDYVQALERADVILDADRRKDIILHDAKDLAFARGL
DLVEDEGLLEEVAGLVERPVVLMGSFEERFLEIPAEAIRATIRANQKCFVLRKSGSDELAPAFVLVSNLIASDGGAAITA
GNERVVRARLSDARFFWETDKATKLEDRLPKLDSIVFHEKLGTQGERVARIAALAAEIAPLVAADPALAERAARLAKADL
VTEMVGEFPELQGLMGRKYAALQGEHDSVAAAIEEHYKPVGPSDRVPTDPVSIAVALADKLDTLVGFWAIDEKPTGSKDP
YALRRAALGVIRAVIERSLRLSLASLLKGRFVSGSDERTADLLAFFADRLKVYLRDQGARHDLIDAVFALPGQDDLLMVV
RRVEALGAFLATDDGKNLLAGYKRAANILRIEEKKDGRAYDEAPDAALAASGQPEERALAEALAAARQEASAAVAAEDFA
GAMRALSRLRAPVDAFFEKVTVNADDPALRKNRLLLLNALRAATREVADFSRIEG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=710, Percent_Identity=38.3098591549296, Blast_Score=399, Evalue=1e-112,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_METC4 (B7KUK7)

Other databases:

- EMBL:   CP001298
- RefSeq:   YP_002422164.1
- ProteinModelPortal:   B7KUK7
- GeneID:   7116063
- GenomeReviews:   CP001298_GR
- KEGG:   mch:Mchl_3416
- HOGENOM:   HBG586599
- OMA:   SNITYGH
- ProtClustDB:   PRK01233
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 75671; Mature: 75540

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLLLELRSEEIPARMQRRAAEDLKKLVTDALVERGFLYEGAKAFATPRRLALHVAGLP
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHCCCHHHEEEEECCC
ARGEAVREERRGPRVGAPEAAVQGFLKSAGLASLDQATTVTDPKKGEFYLAVIERPGRET
CCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHH
LDVLAEILPGIIKSFPWPKSMRWGAASAQPGSLRWVRPLQSIVATFGPETETPEVVPFSV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEC
DGITAGMVTSGHRFLAPEPFEVRRFDDYVQALERADVILDADRRKDIILHDAKDLAFARG
CCCEECCEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCEEEECHHHHHHHHC
LDLVEDEGLLEEVAGLVERPVVLMGSFEERFLEIPAEAIRATIRANQKCFVLRKSGSDEL
CCCCCCCCHHHHHHHHHHCCEEEECCHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCH
APAFVLVSNLIASDGGAAITAGNERVVRARLSDARFFWETDKATKLEDRLPKLDSIVFHE
HHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHCCEEEEECCCCCCHHHHCCHHHHHHHHH
KLGTQGERVARIAALAAEIAPLVAADPALAERAARLAKADLVTEMVGEFPELQGLMGRKY
HHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCH
AALQGEHDSVAAAIEEHYKPVGPSDRVPTDPVSIAVALADKLDTLVGFWAIDEKPTGSKD
HHHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCC
PYALRRAALGVIRAVIERSLRLSLASLLKGRFVSGSDERTADLLAFFADRLKVYLRDQGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
RHDLIDAVFALPGQDDLLMVVRRVEALGAFLATDDGKNLLAGYKRAANILRIEEKKDGRA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEECCCCCCC
YDEAPDAALAASGQPEERALAEALAAARQEASAAVAAEDFAGAMRALSRLRAPVDAFFEK
CCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VTVNADDPALRKNRLLLLNALRAATREVADFSRIEG
HCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDLLLELRSEEIPARMQRRAAEDLKKLVTDALVERGFLYEGAKAFATPRRLALHVAGLP
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHCCCHHHEEEEECCC
ARGEAVREERRGPRVGAPEAAVQGFLKSAGLASLDQATTVTDPKKGEFYLAVIERPGRET
CCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHH
LDVLAEILPGIIKSFPWPKSMRWGAASAQPGSLRWVRPLQSIVATFGPETETPEVVPFSV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEC
DGITAGMVTSGHRFLAPEPFEVRRFDDYVQALERADVILDADRRKDIILHDAKDLAFARG
CCCEECCEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCEEEECHHHHHHHHC
LDLVEDEGLLEEVAGLVERPVVLMGSFEERFLEIPAEAIRATIRANQKCFVLRKSGSDEL
CCCCCCCCHHHHHHHHHHCCEEEECCHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCH
APAFVLVSNLIASDGGAAITAGNERVVRARLSDARFFWETDKATKLEDRLPKLDSIVFHE
HHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHCCEEEEECCCCCCHHHHCCHHHHHHHHH
KLGTQGERVARIAALAAEIAPLVAADPALAERAARLAKADLVTEMVGEFPELQGLMGRKY
HHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCH
AALQGEHDSVAAAIEEHYKPVGPSDRVPTDPVSIAVALADKLDTLVGFWAIDEKPTGSKD
HHHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCC
PYALRRAALGVIRAVIERSLRLSLASLLKGRFVSGSDERTADLLAFFADRLKVYLRDQGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
RHDLIDAVFALPGQDDLLMVVRRVEALGAFLATDDGKNLLAGYKRAANILRIEEKKDGRA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEECCCCCCC
YDEAPDAALAASGQPEERALAEALAAARQEASAAVAAEDFAGAMRALSRLRAPVDAFFEK
CCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VTVNADDPALRKNRLLLLNALRAATREVADFSRIEG
HCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA