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The map label for this gene is fixK [H]

Identifier: 218531321

GI number: 218531321

Start: 3574665

End: 3575411

Strand: Reverse

Name: fixK [H]

Synonym: Mchl_3389

Alternate gene names: 218531321

Gene position: 3575411-3574665 (Counterclockwise)

Preceding gene: 218531323

Following gene: 218531318

Centisome position: 61.88

GC content: 61.98

Gene sequence:

>747_bases
ATGGCTTTAGCCCCCAATCCCCACGCCACAGCCATGCTCATCCGCAAGCTGGAGAGCATCGCCGCCCTCACGGACGAACA
GCGTCAGGCGATCGAGATCCTGCCGGTAAGAACCCACAACCTTGCTGCCCGGCAGGACATCGTGCGCGACGGCGACAAGC
CCTCGCATTGCTGCCTCATCCTCGACGGCTGGGCCTGCCGCTACAAGCTGCTCGGCGATGGCGGACGGCAGATCCTGTCG
TTCCACATTCCCGGTGATGTCCCAGATCTGCAGAGCCTGCACGTCCACACCATGGACCACGGGCTGGCTACCCTGACGAA
GGCGAAAGTGGCCTTCATCCCGCACGAAAACCTACTGGATCTGACACTTCGCCATCCCAGCGTTGGTGCTCTTTTCTGGC
GCGAGACCCTGATCGATGCGGCGGTCTTCCGGCAGTGGATGGTCGGCATGGGCCGGCGTTCCGCCTTCGAGCAGGTCGCG
CACATCTTCTGTGAGCTGTACCTGAAGCTCCAGGCTAAGGGGCTGGCTGAAGATTATCAGTGCCCGCTGCCGATCACGCA
GCTCGATCTAGCCGACGCGCTGGGCCTCACGAGCGTGCACATCAATCGTGTGGTGAAGGACATGCGCGACCGGAACCTGA
TCAGGCTGCACGCCCACACCTTGGTGATTGAAGCCTGGAAGGAGCTTCTGCGGATTGCTGAGTTCGACCCCACGTACCTG
CACCTGGAATATGGAGCGGCCGGATGA

Upstream 100 bases:

>100_bases
AACAACGGCCTATAGTAACAGCTATCATCAGCGGTTCCGACACAGGCGCTGATGGCTGGGAGAGCCGAGCGCTTCCGCCT
GCAATGCAAGGCGAAGCGGC

Downstream 100 bases:

>100_bases
CGCTTCGCCTGCAGCCTGTTCAAGTTGCGACGGGTAGCTCAGACACCTAGAGCGATCTCGTCTTCTCAGACGGGTTCATG
GTTGCGGTTCTGGTTCGGCT

Product: Crp/Fnr family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MALAPNPHATAMLIRKLESIAALTDEQRQAIEILPVRTHNLAARQDIVRDGDKPSHCCLILDGWACRYKLLGDGGRQILS
FHIPGDVPDLQSLHVHTMDHGLATLTKAKVAFIPHENLLDLTLRHPSVGALFWRETLIDAAVFRQWMVGMGRRSAFEQVA
HIFCELYLKLQAKGLAEDYQCPLPITQLDLADALGLTSVHINRVVKDMRDRNLIRLHAHTLVIEAWKELLRIAEFDPTYL
HLEYGAAG

Sequences:

>Translated_248_residues
MALAPNPHATAMLIRKLESIAALTDEQRQAIEILPVRTHNLAARQDIVRDGDKPSHCCLILDGWACRYKLLGDGGRQILS
FHIPGDVPDLQSLHVHTMDHGLATLTKAKVAFIPHENLLDLTLRHPSVGALFWRETLIDAAVFRQWMVGMGRRSAFEQVA
HIFCELYLKLQAKGLAEDYQCPLPITQLDLADALGLTSVHINRVVKDMRDRNLIRLHAHTLVIEAWKELLRIAEFDPTYL
HLEYGAAG
>Mature_247_residues
ALAPNPHATAMLIRKLESIAALTDEQRQAIEILPVRTHNLAARQDIVRDGDKPSHCCLILDGWACRYKLLGDGGRQILSF
HIPGDVPDLQSLHVHTMDHGLATLTKAKVAFIPHENLLDLTLRHPSVGALFWRETLIDAAVFRQWMVGMGRRSAFEQVAH
IFCELYLKLQAKGLAEDYQCPLPITQLDLADALGLTSVHINRVVKDMRDRNLIRLHAHTLVIEAWKELLRIAEFDPTYLH
LEYGAAG

Specific function: FixK is a protein that regulates nitrogen fixation genes both positively and negatively. FixK appears to repress its own expression and that of nifA. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 27894; Mature: 27763

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAPNPHATAMLIRKLESIAALTDEQRQAIEILPVRTHNLAARQDIVRDGDKPSHCCLI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEE
LDGWACRYKLLGDGGRQILSFHIPGDVPDLQSLHVHTMDHGLATLTKAKVAFIPHENLLD
EECCEEEEEEECCCCEEEEEEECCCCCCCCHHEEEEHHHHHHHHHHHHEEEECCCCCCEE
LTLRHPSVGALFWRETLIDAAVFRQWMVGMGRRSAFEQVAHIFCELYLKLQAKGLAEDYQ
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
CPLPITQLDLADALGLTSVHINRVVKDMRDRNLIRLHAHTLVIEAWKELLRIAEFDPTYL
CCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEE
HLEYGAAG
EEECCCCC
>Mature Secondary Structure 
ALAPNPHATAMLIRKLESIAALTDEQRQAIEILPVRTHNLAARQDIVRDGDKPSHCCLI
CCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEE
LDGWACRYKLLGDGGRQILSFHIPGDVPDLQSLHVHTMDHGLATLTKAKVAFIPHENLLD
EECCEEEEEEECCCCEEEEEEECCCCCCCCHHEEEEHHHHHHHHHHHHEEEECCCCCCEE
LTLRHPSVGALFWRETLIDAAVFRQWMVGMGRRSAFEQVAHIFCELYLKLQAKGLAEDYQ
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
CPLPITQLDLADALGLTSVHINRVVKDMRDRNLIRLHAHTLVIEAWKELLRIAEFDPTYL
CCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEE
HLEYGAAG
EEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2663474; 11481432 [H]