Click here to switch to the map view.

The map label for this gene is gatB

Identifier: 218531237

GI number: 218531237

Start: 3468785

End: 3470257

Strand: Reverse

Name: gatB

Synonym: Mchl_3288

Alternate gene names: 218531237

Gene position: 3470257-3468785 (Counterclockwise)

Preceding gene: 218531238

Following gene: 218531236

Centisome position: 60.06

GC content: 66.4

Gene sequence:

>1473_bases
ATGACCGAGCGCGTCGATCCGAAGAAGCTCATCAAGGGCGGCCTCCACGACTGGGAGGTCGTGATCGGCATGGAGATCCA
CGCCCAGGTCACGAGCCGCTCCAAGCTGTTCTCCGGCGCCTCGACCGAATTCGGCGGCGAGCCCAACGACCACGTCTCGC
TGGTCGACGCGGCCATGCCCGGCATGCTGCCGGTCATCAACGAGGAATGCGTCGCCCAGGCCGTGCGCACCGGTCTCGGG
CTGAAGGCCCAGATCAACCTTCGCTCGGTGTTCGACCGGAAGAACTACTTCTATCCGGATCTGCCGCAGGGCTACCAGAT
CTCGCAGTACAAGGACCCGATCGTCGGCGAGGGCGAGGTGCTGGTCGATCTGCCCGAGGGCGAGTCGATCACGGTGGGCA
TCGAGCGCCTGCACCTCGAACAGGATGCCGGCAAGTCGCTGCACGATCAGGATCCGACCAAGAGCTTCGTCGATCTCAAC
CGCTCCGGCGTGGCGCTGATGGAGATCGTCTCCCGGCCCGATCTGCGCTCCTCGGAGGAGGCCCGGGCCTACGTGACGAA
GCTGCGCACGATCCTGCGCTATCTCGGCACCTGCGACGGCGACATGGAGAAGGGGAGCCTTCGCGCCGACGTGAACGTCT
CCGTGCGCCGCCCCGGTGAGCCGCTCGGCACGCGCTGCGAGATCAAGAACGTCAACTCGATCCGCTTCATCGGTCAGGCC
ATCGAGACCGAGGCGCGCCGCCAGATCGCGATCCTGGAGGACGGCGGCAAAATCGATCAGGAGACGCGCCTCTACGACCC
GGGCAAGGGCGAGACCCGCTCGATGCGCTCGAAGGAAGAGGCGCACGATTATCGCTACTTCCCCGATCCCGACCTGTTGC
CGCTCGAATTCGATCAGGCCTACGTCGATGCTTTGGCCTCGGGCCTGCCGGAACTGCCGGACGCCAAGAAGGCGCGCTTC
ATCAAGGATTTCGGCCTTTCCGCCTACGATGCCGGCGTGCTCGTCGCCGAGCGGGCCTCGGCCGACTACTTCGAGGCGGT
CGCCCGCGGGCGCGACGGCAAGGCCGCCGCGAACTGGGTCATCAACGAGCTGTTCGGCCGCCTCAACAAGGAGGGGCGCA
GCATCGAGGACACGCCGGTCTCCGCCGAGCAGCTCGGCACCATCGTCGATCTGATCGGCGATGGCGTGATCTCGGGCAAG
ATCGCCAAGGACCTGTTCGAGATCGTCTGGTCCGAGGGCGGCGACCCGCGCGCGATCGTCGAGGCCCGCGGCATGAAGCA
GGTCACCGATACCGGCGCCATTGAGGCGGCGGTGGACGCGATCATCGCCGCCAACCCCGACAAGGTCGAGCAGGCCAAGG
CCAAGCCGACCCTGCTCGGCTGGTTCGTCGGCCAGACCATGAAGGCGACGGGCGGCAAGGCCAATCCGGCCGCGGTCAAC
GCGCTGCTGAAGGACAAACTCGGTATCGAGTAG

Upstream 100 bases:

>100_bases
CGGCCAGGCGCGGCTTCACCGGCTTGACCCGTCCGGCACGGTCTCCCTACACACTCGGACAGGCGCCGATCCGGCGGCGC
TGGCACTGAAGTCGAAGAGA

Downstream 100 bases:

>100_bases
GTCCGGCGGTCGCACCTAGAGTGCGTTCATACCAAGGGGCCACCGGTTCGTCGGAAGAATGCGCGGCAACGCGATGACCT
CGCTCGCTTTTGCAACGAAG

Product: glutamyl-tRNA(Gln) amidotransferase subunit beta

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTERVDPKKLIKGGLHDWEVVIGMEIHAQVTSRSKLFSGASTEFGGEPNDHVSLVDAAMPGMLPVINEECVAQAVRTGLG
LKAQINLRSVFDRKNYFYPDLPQGYQISQYKDPIVGEGEVLVDLPEGESITVGIERLHLEQDAGKSLHDQDPTKSFVDLN
RSGVALMEIVSRPDLRSSEEARAYVTKLRTILRYLGTCDGDMEKGSLRADVNVSVRRPGEPLGTRCEIKNVNSIRFIGQA
IETEARRQIAILEDGGKIDQETRLYDPGKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQAYVDALASGLPELPDAKKARF
IKDFGLSAYDAGVLVAERASADYFEAVARGRDGKAAANWVINELFGRLNKEGRSIEDTPVSAEQLGTIVDLIGDGVISGK
IAKDLFEIVWSEGGDPRAIVEARGMKQVTDTGAIEAAVDAIIAANPDKVEQAKAKPTLLGWFVGQTMKATGGKANPAAVN
ALLKDKLGIE

Sequences:

>Translated_490_residues
MTERVDPKKLIKGGLHDWEVVIGMEIHAQVTSRSKLFSGASTEFGGEPNDHVSLVDAAMPGMLPVINEECVAQAVRTGLG
LKAQINLRSVFDRKNYFYPDLPQGYQISQYKDPIVGEGEVLVDLPEGESITVGIERLHLEQDAGKSLHDQDPTKSFVDLN
RSGVALMEIVSRPDLRSSEEARAYVTKLRTILRYLGTCDGDMEKGSLRADVNVSVRRPGEPLGTRCEIKNVNSIRFIGQA
IETEARRQIAILEDGGKIDQETRLYDPGKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQAYVDALASGLPELPDAKKARF
IKDFGLSAYDAGVLVAERASADYFEAVARGRDGKAAANWVINELFGRLNKEGRSIEDTPVSAEQLGTIVDLIGDGVISGK
IAKDLFEIVWSEGGDPRAIVEARGMKQVTDTGAIEAAVDAIIAANPDKVEQAKAKPTLLGWFVGQTMKATGGKANPAAVN
ALLKDKLGIE
>Mature_489_residues
TERVDPKKLIKGGLHDWEVVIGMEIHAQVTSRSKLFSGASTEFGGEPNDHVSLVDAAMPGMLPVINEECVAQAVRTGLGL
KAQINLRSVFDRKNYFYPDLPQGYQISQYKDPIVGEGEVLVDLPEGESITVGIERLHLEQDAGKSLHDQDPTKSFVDLNR
SGVALMEIVSRPDLRSSEEARAYVTKLRTILRYLGTCDGDMEKGSLRADVNVSVRRPGEPLGTRCEIKNVNSIRFIGQAI
ETEARRQIAILEDGGKIDQETRLYDPGKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQAYVDALASGLPELPDAKKARFI
KDFGLSAYDAGVLVAERASADYFEAVARGRDGKAAANWVINELFGRLNKEGRSIEDTPVSAEQLGTIVDLIGDGVISGKI
AKDLFEIVWSEGGDPRAIVEARGMKQVTDTGAIEAAVDAIIAANPDKVEQAKAKPTLLGWFVGQTMKATGGKANPAAVNA
LLKDKLGIE

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=501, Percent_Identity=39.7205588822355, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI115534094, Length=300, Percent_Identity=41, Blast_Score=214, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=528, Percent_Identity=30.6818181818182, Blast_Score=219, Evalue=5e-58,
Organism=Drosophila melanogaster, GI45550808, Length=480, Percent_Identity=38.5416666666667, Blast_Score=334, Evalue=8e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_METC4 (B7KTR5)

Other databases:

- EMBL:   CP001298
- RefSeq:   YP_002422053.1
- ProteinModelPortal:   B7KTR5
- SMR:   B7KTR5
- GeneID:   7117055
- GenomeReviews:   CP001298_GR
- KEGG:   mch:Mchl_3288
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   CLSK2331496
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53452; Mature: 53321

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERVDPKKLIKGGLHDWEVVIGMEIHAQVTSRSKLFSGASTEFGGEPNDHVSLVDAAMP
CCCCCCHHHHHHCCCCCEEEEEEEEEEEEHHHHHHHHCCCCCCCCCCCCCCEEHHHHHCC
GMLPVINEECVAQAVRTGLGLKAQINLRSVFDRKNYFYPDLPQGYQISQYKDPIVGEGEV
CCCHHHCHHHHHHHHHHCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCE
LVDLPEGESITVGIERLHLEQDAGKSLHDQDPTKSFVDLNRSGVALMEIVSRPDLRSSEE
EEECCCCCEEEEEHHHHEEHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCCCCHH
ARAYVTKLRTILRYLGTCDGDMEKGSLRADVNVSVRRPGEPLGTRCEIKNVNSIRFIGQA
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCHHHHHHHH
IETEARRQIAILEDGGKIDQETRLYDPGKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQA
HHHHHHCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
YVDALASGLPELPDAKKARFIKDFGLSAYDAGVLVAERASADYFEAVARGRDGKAAANWV
HHHHHHHCCCCCCCHHHHHHHHHHCCCHHCCCEEEEECCCHHHHHHHHCCCCCCHHHHHH
INELFGRLNKEGRSIEDTPVSAEQLGTIVDLIGDGVISGKIAKDLFEIVWSEGGDPRAIV
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEHH
EARGMKQVTDTGAIEAAVDAIIAANPDKVEQAKAKPTLLGWFVGQTMKATGGKANPAAVN
HHCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCCEEEHHHCCHHHCCCCCCCHHHHH
ALLKDKLGIE
HHHHHHCCCC
>Mature Secondary Structure 
TERVDPKKLIKGGLHDWEVVIGMEIHAQVTSRSKLFSGASTEFGGEPNDHVSLVDAAMP
CCCCCHHHHHHCCCCCEEEEEEEEEEEEHHHHHHHHCCCCCCCCCCCCCCEEHHHHHCC
GMLPVINEECVAQAVRTGLGLKAQINLRSVFDRKNYFYPDLPQGYQISQYKDPIVGEGEV
CCCHHHCHHHHHHHHHHCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCE
LVDLPEGESITVGIERLHLEQDAGKSLHDQDPTKSFVDLNRSGVALMEIVSRPDLRSSEE
EEECCCCCEEEEEHHHHEEHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCCCCHH
ARAYVTKLRTILRYLGTCDGDMEKGSLRADVNVSVRRPGEPLGTRCEIKNVNSIRFIGQA
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCHHHHHHHH
IETEARRQIAILEDGGKIDQETRLYDPGKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQA
HHHHHHCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
YVDALASGLPELPDAKKARFIKDFGLSAYDAGVLVAERASADYFEAVARGRDGKAAANWV
HHHHHHHCCCCCCCHHHHHHHHHHCCCHHCCCEEEEECCCHHHHHHHHCCCCCCHHHHHH
INELFGRLNKEGRSIEDTPVSAEQLGTIVDLIGDGVISGKIAKDLFEIVWSEGGDPRAIV
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEHH
EARGMKQVTDTGAIEAAVDAIIAANPDKVEQAKAKPTLLGWFVGQTMKATGGKANPAAVN
HHCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCCEEEHHHCCHHHCCCCCCCHHHHH
ALLKDKLGIE
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA