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The map label for this gene is spoT [H]

Identifier: 218531192

GI number: 218531192

Start: 3425199

End: 3425759

Strand: Reverse

Name: spoT [H]

Synonym: Mchl_3243

Alternate gene names: 218531192

Gene position: 3425759-3425199 (Counterclockwise)

Preceding gene: 218531193

Following gene: 218531191

Centisome position: 59.29

GC content: 71.48

Gene sequence:

>561_bases
GTGAGCGGGCTCGATCCGGATCGTCGGGGGGACGTCGCGCTCGTGTCGCGCGCGGCCGATTTCGCCGCCCGCCGGCACGC
GGACCAGCAGAGGAAGGGCGCCGCGCGCGAGCCCTACCTCAACCATCTGGCGGAAGTTGCCTGCCTTCTCGCGGAGACGA
TGGGGGAACCCGACGCCGCCCTCGTGGCCGCCGGCTGGCTCCACGACACGCTCGAAGACACGCAGACAGACCTCGAAGAG
CTGGAGCGCCTGTTCGGCCCGCGCGTCGCCGCCCTCGTCGCGGAGGTGACGGACGACAAGTCTCTCCCGAAAGCGGAGCG
CAAGCGGCGGCAGGTCGAGGCGGCGCCGCACAAGTCGGCGGGCGCCAAGGCGATCAAGGTCGCCGACAAGACCAGCAATC
TCCGCTCGCTGACGGCGAGCCCGCCGGAGGGCTGGGAGCAGCAGCGGATCGCCGACTACATCGCCTGGGCGGAAGAAGTC
GTGGCGGGCTGCCGCGGCACCAATGCCCCCCTGGAAGATCGCTTCGACCGCGCGGTCGCCGAGGCGCGAGGGACTCTTTA
G

Upstream 100 bases:

>100_bases
CGGTGCGCTTCGAGCCGGGCTCGACCCGCGAGGTGGCGCTGGTGCCGCTCTCGGGCGACCGGAAGGTCTACGGCTTCCGC
GGCGACGTGATGGGGCCGCT

Downstream 100 bases:

>100_bases
ACATGGCCGACATCCGCAAGCCCGCCGCCCTCCCCCGCGCCGCCTACGCCGCCATGTTCGGGCCGACCACCGGGGACCGT
ATCCGGCTGGCCGACACCAG

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MSGLDPDRRGDVALVSRAADFAARRHADQQRKGAAREPYLNHLAEVACLLAETMGEPDAALVAAGWLHDTLEDTQTDLEE
LERLFGPRVAALVAEVTDDKSLPKAERKRRQVEAAPHKSAGAKAIKVADKTSNLRSLTASPPEGWEQQRIADYIAWAEEV
VAGCRGTNAPLEDRFDRAVAEARGTL

Sequences:

>Translated_186_residues
MSGLDPDRRGDVALVSRAADFAARRHADQQRKGAAREPYLNHLAEVACLLAETMGEPDAALVAAGWLHDTLEDTQTDLEE
LERLFGPRVAALVAEVTDDKSLPKAERKRRQVEAAPHKSAGAKAIKVADKTSNLRSLTASPPEGWEQQRIADYIAWAEEV
VAGCRGTNAPLEDRFDRAVAEARGTL
>Mature_185_residues
SGLDPDRRGDVALVSRAADFAARRHADQQRKGAAREPYLNHLAEVACLLAETMGEPDAALVAAGWLHDTLEDTQTDLEEL
ERLFGPRVAALVAEVTDDKSLPKAERKRRQVEAAPHKSAGAKAIKVADKTSNLRSLTASPPEGWEQQRIADYIAWAEEVV
AGCRGTNAPLEDRFDRAVAEARGTL

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=126, Percent_Identity=55.5555555555556, Blast_Score=132, Evalue=2e-31,
Organism=Escherichia coli, GI1790082, Length=143, Percent_Identity=36.3636363636364, Blast_Score=67, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25143118, Length=170, Percent_Identity=43.5294117647059, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24650996, Length=166, Percent_Identity=46.9879518072289, Blast_Score=150, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 20235; Mature: 20104

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLDPDRRGDVALVSRAADFAARRHADQQRKGAAREPYLNHLAEVACLLAETMGEPDAA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
LVAAGWLHDTLEDTQTDLEELERLFGPRVAALVAEVTDDKSLPKAERKRRQVEAAPHKSA
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
GAKAIKVADKTSNLRSLTASPPEGWEQQRIADYIAWAEEVVAGCRGTNAPLEDRFDRAVA
CCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
EARGTL
HHCCCC
>Mature Secondary Structure 
SGLDPDRRGDVALVSRAADFAARRHADQQRKGAAREPYLNHLAEVACLLAETMGEPDAA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
LVAAGWLHDTLEDTQTDLEELERLFGPRVAALVAEVTDDKSLPKAERKRRQVEAAPHKSA
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
GAKAIKVADKTSNLRSLTASPPEGWEQQRIADYIAWAEEVVAGCRGTNAPLEDRFDRAVA
CCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
EARGTL
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]