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The map label for this gene is tilS/hprT [H]

Identifier: 218531037

GI number: 218531037

Start: 3268221

End: 3268790

Strand: Reverse

Name: tilS/hprT [H]

Synonym: Mchl_3087

Alternate gene names: 218531037

Gene position: 3268790-3268221 (Counterclockwise)

Preceding gene: 218531038

Following gene: 218531036

Centisome position: 56.57

GC content: 66.32

Gene sequence:

>570_bases
ATGAGCAACGCATCGAAACGCGTCCGCGTCCTGTTCGACGAGGCGGCGATTGCCAAGCGCAACGACGAGTTGGCCGACGA
GATCGTCGCGGCCAAGCCCGAGAACCTGCTCGTCGTCGCGGTGCTCAAGGGCAGCTTCATGTTCGCCGCCGACCTGCTGC
GCTCGCTCCACCGGGCCGGGCTCTCGCCGCAGGTCGAGTTCGTGCATCTCTCCAGCTACCGCAACTCCACCGTTTCCTCG
GGCCAGGTCGAGATCCTGCGCGACGTTCAGAGCGAGGTGCGCGGGCGTGACGTGCTGCTGGTCGACGACATCCTCGAATC
CGGCCGCACCGTCGTCTTCGCCAAGGATCTGTTGATGGCCCGCGGCGCCAAGCGTGTGCTGACCGCGGTGCTCTTGGAGA
AGCCGGGCAAGCGTGCCGTGACGATCGACGCGGATTTCGTCGGTTTCACCTGCCCGGACGTGTTCGTCGTCGGCTACGGC
ATGGACGCCGCCCACGCCTTCCGCCAACTCCCCTTCGTCGGCTTGGTAGACTACGACAGCAGCGACGCGGACCTGTTCGG
CGGCACCTGA

Upstream 100 bases:

>100_bases
GTAGCGCTGGCGGAGCCCGCTGCGCATGGTATGATGCGGCGTGGCATGGCCGAGGGCGGTTCGTGCCGTTGGAACATTCC
GCGAAACCATCACGAGCCGC

Downstream 100 bases:

>100_bases
CCGTCAATCCCGGTCAGGCAAATTCCTGATAAGAACGCGAGACTTGCTCGAATTCGGCCGCCGATGGAACTCTGTCGGCG
CATGAGGCTTGACAGGCCGC

Product: hypoxanthine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MSNASKRVRVLFDEAAIAKRNDELADEIVAAKPENLLVVAVLKGSFMFAADLLRSLHRAGLSPQVEFVHLSSYRNSTVSS
GQVEILRDVQSEVRGRDVLLVDDILESGRTVVFAKDLLMARGAKRVLTAVLLEKPGKRAVTIDADFVGFTCPDVFVVGYG
MDAAHAFRQLPFVGLVDYDSSDADLFGGT

Sequences:

>Translated_189_residues
MSNASKRVRVLFDEAAIAKRNDELADEIVAAKPENLLVVAVLKGSFMFAADLLRSLHRAGLSPQVEFVHLSSYRNSTVSS
GQVEILRDVQSEVRGRDVLLVDDILESGRTVVFAKDLLMARGAKRVLTAVLLEKPGKRAVTIDADFVGFTCPDVFVVGYG
MDAAHAFRQLPFVGLVDYDSSDADLFGGT
>Mature_188_residues
SNASKRVRVLFDEAAIAKRNDELADEIVAAKPENLLVVAVLKGSFMFAADLLRSLHRAGLSPQVEFVHLSSYRNSTVSSG
QVEILRDVQSEVRGRDVLLVDDILESGRTVVFAKDLLMARGAKRVLTAVLLEKPGKRAVTIDADFVGFTCPDVFVVGYGM
DAAHAFRQLPFVGLVDYDSSDADLFGGT

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=176, Percent_Identity=34.6590909090909, Blast_Score=89, Evalue=2e-18,
Organism=Homo sapiens, GI9910262, Length=176, Percent_Identity=31.8181818181818, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI87081689, Length=172, Percent_Identity=39.5348837209302, Blast_Score=132, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17510817, Length=169, Percent_Identity=38.4615384615385, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 20545; Mature: 20414

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNASKRVRVLFDEAAIAKRNDELADEIVAAKPENLLVVAVLKGSFMFAADLLRSLHRAG
CCCCCCEEEEEECCHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCC
LSPQVEFVHLSSYRNSTVSSGQVEILRDVQSEVRGRDVLLVDDILESGRTVVFAKDLLMA
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHCCCEEEEEHHHHHH
RGAKRVLTAVLLEKPGKRAVTIDADFVGFTCPDVFVVGYGMDAAHAFRQLPFVGLVDYDS
CCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHCCEEEEEECCC
SDADLFGGT
CCCCCCCCC
>Mature Secondary Structure 
SNASKRVRVLFDEAAIAKRNDELADEIVAAKPENLLVVAVLKGSFMFAADLLRSLHRAG
CCCCCEEEEEECCHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCC
LSPQVEFVHLSSYRNSTVSSGQVEILRDVQSEVRGRDVLLVDDILESGRTVVFAKDLLMA
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHCCCEEEEEHHHHHH
RGAKRVLTAVLLEKPGKRAVTIDADFVGFTCPDVFVVGYGMDAAHAFRQLPFVGLVDYDS
CCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHCCEEEEEECCC
SDADLFGGT
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]