Click here to switch to the map view.

The map label for this gene is queF

Identifier: 218530961

GI number: 218530961

Start: 3181592

End: 3182053

Strand: Reverse

Name: queF

Synonym: Mchl_3011

Alternate gene names: 218530961

Gene position: 3182053-3181592 (Counterclockwise)

Preceding gene: 218530970

Following gene: 218530954

Centisome position: 55.07

GC content: 68.18

Gene sequence:

>462_bases
ATGGATTCGATCGAGACCCACGCCAAGCAACTCGGACAGCAGACGCCGCTGCCGGCCTCGCCCGAGGCTGCGCAGCTCGA
CCGCGTGCCCAACCCTCATGCCGACACCGATTACCTCGCCCGCTTCACCGCGCCGGAATTCACCTCGCTCTGCCCGGTGA
CGGGCCAGCCGGACTTCGCGACGCTTGTCATCGACTACGTGCCGGACCGCTGGCTCGTGGAGTCGAAATCGCTGAAGCTC
TACCTCGGCGCCTTCCGCAACCACGGCGCCTTCCACGAGGATTGCACCGTCGGCATCGGGCGGCGGCTCGTCGCATTGCT
CGAACCGCGCTGGCTGCGCATCGGCGGCTACTGGTATCCGCGCGGCGGCATTCCGATCGACGTGTTCTGGCAGACGGGTG
AGCCCCTGAAATCGGTCTGGCTGCCGGACCAGGGGGTCGCGCCCTATCGCGGCCGCGGCTGA

Upstream 100 bases:

>100_bases
TTCCGGGACCGGTTCTTGGTCCAGTTCGGTTGATCGGGGAGAATCCCTGGCATTCTCCCGGCGCCATAGGCGCCAGGGGC
GTTGCCGGGTATAGGCCCCC

Downstream 100 bases:

>100_bases
GCGACCGCGCCTCCGCCGTCAATGCTGCTCGGCCAGCACGCGCACGGTGCGTGCGATAAAGCGCTCCTGTGCTTCGATCG
GCCCGCCGCGAATGACGGCG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MDSIETHAKQLGQQTPLPASPEAAQLDRVPNPHADTDYLARFTAPEFTSLCPVTGQPDFATLVIDYVPDRWLVESKSLKL
YLGAFRNHGAFHEDCTVGIGRRLVALLEPRWLRIGGYWYPRGGIPIDVFWQTGEPLKSVWLPDQGVAPYRGRG

Sequences:

>Translated_153_residues
MDSIETHAKQLGQQTPLPASPEAAQLDRVPNPHADTDYLARFTAPEFTSLCPVTGQPDFATLVIDYVPDRWLVESKSLKL
YLGAFRNHGAFHEDCTVGIGRRLVALLEPRWLRIGGYWYPRGGIPIDVFWQTGEPLKSVWLPDQGVAPYRGRG
>Mature_153_residues
MDSIETHAKQLGQQTPLPASPEAAQLDRVPNPHADTDYLARFTAPEFTSLCPVTGQPDFATLVIDYVPDRWLVESKSLKL
YLGAFRNHGAFHEDCTVGIGRRLVALLEPRWLRIGGYWYPRGGIPIDVFWQTGEPLKSVWLPDQGVAPYRGRG

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_METC4 (B7KRB3)

Other databases:

- EMBL:   CP001298
- RefSeq:   YP_002421777.1
- ProteinModelPortal:   B7KRB3
- SMR:   B7KRB3
- GeneID:   7118208
- GenomeReviews:   CP001298_GR
- KEGG:   mch:Mchl_3011
- HOGENOM:   HBG294103
- OMA:   HEDCTVA
- ProtClustDB:   PRK13258
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 17130; Mature: 17130

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSIETHAKQLGQQTPLPASPEAAQLDRVPNPHADTDYLARFTAPEFTSLCPVTGQPDFA
CCCHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCEE
TLVIDYVPDRWLVESKSLKLYLGAFRNHGAFHEDCTVGIGRRLVALLEPRWLRIGGYWYP
EEEEECCCCCEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCEEEC
RGGIPIDVFWQTGEPLKSVWLPDQGVAPYRGRG
CCCCEEEEEECCCCCHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MDSIETHAKQLGQQTPLPASPEAAQLDRVPNPHADTDYLARFTAPEFTSLCPVTGQPDFA
CCCHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCEE
TLVIDYVPDRWLVESKSLKLYLGAFRNHGAFHEDCTVGIGRRLVALLEPRWLRIGGYWYP
EEEEECCCCCEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCEEEC
RGGIPIDVFWQTGEPLKSVWLPDQGVAPYRGRG
CCCCEEEEEECCCCCHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA