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The map label for this gene is nth [H]

Identifier: 218530896

GI number: 218530896

Start: 3107464

End: 3108228

Strand: Reverse

Name: nth [H]

Synonym: Mchl_2945

Alternate gene names: 218530896

Gene position: 3108228-3107464 (Counterclockwise)

Preceding gene: 218530897

Following gene: 218530894

Centisome position: 53.8

GC content: 68.1

Gene sequence:

>765_bases
ATGACGCTTTTCCCCGATACAGCCCTCCCGGCGGCAGCCCGCCATCGCCGCCGCGAGGCACCGGCCGCCGCGTCCGAGGC
TCTGAAGCACAAGGCGCTGGAGATCCACCGCCGCCTCTGCGGCGTCTACGAATGCCCGATCCCGTATTTCCACAGCCTCG
ATCCGCTGTCGGAGCTGATCTCCTCGCTGCTCTCACACCGGACCCGCAACGCCGATTCAGGGCGTGCCTTCAAGGCGCTG
CGGGCTCGCTGGCCCGATTGGACGGAGATCGATGCGGCGTCGGTCAAGGAGATCGAGGCGACGATCCACGGCGTCACTTG
GCCGGAGCTGAAGGCGCCACGCATCAAGGCGGTGCTCGCCGCGGTGCAGGAGCGGGTCGGTGCACTCTCCCTCGATTTCT
TGGGGGATATGAGCGTCGATGAGGCGCGCGGCTGGCTCGAAGCGATCCCCGGCATCGGGCCGAAGACGAGTGCCGCGGTG
CTGTCGTTCTCGACGCTGCGAATGCCGGCCCTGCCGGTGGACAGCCACCATCACCGCGTCGCCCAGCGCACGGGGCTGAT
CGGTGCCAAGGTCGATGTCGGGCCGAGCCACGCGGTTCTGCGGGCACAGCTTCCGGACGATTGGTCGGCGCAGAAGCTCT
ACGACAATCACGAGGTGCTGATGCTTCACGGCCAGCGCGTCTGCTTTCATCGCTCGCCGGCCTGCGGACGCTGCGTGCTG
CTCGACATCTGCCCGACAGGTCAGGCGAGGAGGGCGGCGGGCTGA

Upstream 100 bases:

>100_bases
ATCAACATCTACGCGCTGCCGAAAGAGCCGGCGAAGGAGCCGGCGAAGTAGGGTTGTCGCGGGCCGGGAACCGCCGAATC
CCTTCGCGGATTCGGCTTTC

Downstream 100 bases:

>100_bases
CGCTCTATCTGGTCGCGTGTATCTGCCTCTCTCACGTCCAATAGAGGATGCGCGCCGTCGGCAGGCGTTCGGCGATCCGC
CCGGTGAGGTGAGCCCGCAT

Product: endonuclease III FCL domain protein

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTLFPDTALPAAARHRRREAPAAASEALKHKALEIHRRLCGVYECPIPYFHSLDPLSELISSLLSHRTRNADSGRAFKAL
RARWPDWTEIDAASVKEIEATIHGVTWPELKAPRIKAVLAAVQERVGALSLDFLGDMSVDEARGWLEAIPGIGPKTSAAV
LSFSTLRMPALPVDSHHHRVAQRTGLIGAKVDVGPSHAVLRAQLPDDWSAQKLYDNHEVLMLHGQRVCFHRSPACGRCVL
LDICPTGQARRAAG

Sequences:

>Translated_254_residues
MTLFPDTALPAAARHRRREAPAAASEALKHKALEIHRRLCGVYECPIPYFHSLDPLSELISSLLSHRTRNADSGRAFKAL
RARWPDWTEIDAASVKEIEATIHGVTWPELKAPRIKAVLAAVQERVGALSLDFLGDMSVDEARGWLEAIPGIGPKTSAAV
LSFSTLRMPALPVDSHHHRVAQRTGLIGAKVDVGPSHAVLRAQLPDDWSAQKLYDNHEVLMLHGQRVCFHRSPACGRCVL
LDICPTGQARRAAG
>Mature_253_residues
TLFPDTALPAAARHRRREAPAAASEALKHKALEIHRRLCGVYECPIPYFHSLDPLSELISSLLSHRTRNADSGRAFKALR
ARWPDWTEIDAASVKEIEATIHGVTWPELKAPRIKAVLAAVQERVGALSLDFLGDMSVDEARGWLEAIPGIGPKTSAAVL
SFSTLRMPALPVDSHHHRVAQRTGLIGAKVDVGPSHAVLRAQLPDDWSAQKLYDNHEVLMLHGQRVCFHRSPACGRCVLL
DICPTGQARRAAG

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Escherichia coli, GI1787920, Length=216, Percent_Identity=28.2407407407407, Blast_Score=69, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45550361, Length=196, Percent_Identity=26.0204081632653, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 27837; Mature: 27706

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLFPDTALPAAARHRRREAPAAASEALKHKALEIHRRLCGVYECPIPYFHSLDPLSELI
CCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCHHHHHH
SSLLSHRTRNADSGRAFKALRARWPDWTEIDAASVKEIEATIHGVTWPELKAPRIKAVLA
HHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
AVQERVGALSLDFLGDMSVDEARGWLEAIPGIGPKTSAAVLSFSTLRMPALPVDSHHHRV
HHHHHHCCEEHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHH
AQRTGLIGAKVDVGPSHAVLRAQLPDDWSAQKLYDNHEVLMLHGQRVCFHRSPACGRCVL
HHHHCCCEEEEECCCCCEEEEECCCCCCCHHHHCCCCCEEEEECCEEEEECCCCCCCEEE
LDICPTGQARRAAG
EEECCCCCCCCCCC
>Mature Secondary Structure 
TLFPDTALPAAARHRRREAPAAASEALKHKALEIHRRLCGVYECPIPYFHSLDPLSELI
CCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCHHHHHH
SSLLSHRTRNADSGRAFKALRARWPDWTEIDAASVKEIEATIHGVTWPELKAPRIKAVLA
HHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
AVQERVGALSLDFLGDMSVDEARGWLEAIPGIGPKTSAAVLSFSTLRMPALPVDSHHHRV
HHHHHHCCEEHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHH
AQRTGLIGAKVDVGPSHAVLRAQLPDDWSAQKLYDNHEVLMLHGQRVCFHRSPACGRCVL
HHHHCCCEEEEECCCCCEEEEECCCCCCCHHHHCCCCCEEEEECCEEEEECCCCCCCEEE
LDICPTGQARRAAG
EEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]