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The map label for this gene is azoB [H]

Identifier: 218236125

GI number: 218236125

Start: 3047891

End: 3048712

Strand: Reverse

Name: azoB [H]

Synonym: BCB4264_A3166

Alternate gene names: 218236125

Gene position: 3048712-3047891 (Counterclockwise)

Preceding gene: 218234841

Following gene: 218231250

Centisome position: 56.26

GC content: 30.78

Gene sequence:

>822_bases
ATGATTTTAGTAACAGGTGCTACAGGGAATGTAGGAAAAGAGGTTGTAAAATGCCTAATAAAAAGAAATGCTACTTTTAA
AGTCACAACGCGCAGTAGAGAAACTGAAGGAGTGTATTTTGATTTTGAAAATCCTTCATCTGTAAAGGCGGCTTTAACAG
GTGTTACTAAAATCTTTCTAATAAGACCACCACATCTAGCTGATGCAAAAAAGTATTTCCAGCCTGTTGTAGATGTCGCA
AAAGAAATAGGTATAAAACACATTGTATTTTTATCTTTATTAGGTGTAGAAAAAAATCCGATAGTACCTCATGCTAAAAT
TGAAGCTATGATAAAGGCTTCTGGGATCCCTTACACATTCCTAAGACCGAGTTTTTTTATGCAAAATTTATTATCACAAC
ATGGTGATGAATTAAGAAATGAAAAAATAATTGAAGTACCTGCTGGTAAAGGGGAAACAAGTTTTATAGATGTACGGGAT
ATTGGAGAAGTTGCAGCTAAAATATTAACCGAAGAGGGACATGAATTTAAAGCTTATTCTTTAACGGGAAATGAGGCATT
AACATATTATGAAGTTGCTGCTATTATTTCAAAAATAATAAATAAAGATATTACTTATACAAATCCAAGTATATTTAAAT
TTAGAAAAAGAATGATTCAAAAGGGACTTAACAGTGACTTTATTTGGGTTATGATTGGAATATATACAACTGCAAAATTA
GGGTTAGCTAAAAAAGTCACATCAGATTTATCGAATTTACTAGATAGAAGTCCAATAACTATGGAAACGTTTGTTTACGA
TTACAAAGAATTATTAATATAA

Upstream 100 bases:

>100_bases
AAAATGCTACAGCTAGTATTCCTAAAGAGGAAGTAGAAAATACAGTTCGAGTTTTGCAAAGTATGATTCAAAATTTACAA
AAAAATAAAGGGTGATTAAA

Downstream 100 bases:

>100_bases
TAATGCAAAAGTAAAAAAAGAGGATTATTCACATAAAGAATCTATTATGTTTTAAGAGTAAGAGGGGATAGCCATGCAAA
ACAAAATTATCGTTATGTTT

Product: NmrA family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MILVTGATGNVGKEVVKCLIKRNATFKVTTRSRETEGVYFDFENPSSVKAALTGVTKIFLIRPPHLADAKKYFQPVVDVA
KEIGIKHIVFLSLLGVEKNPIVPHAKIEAMIKASGIPYTFLRPSFFMQNLLSQHGDELRNEKIIEVPAGKGETSFIDVRD
IGEVAAKILTEEGHEFKAYSLTGNEALTYYEVAAIISKIINKDITYTNPSIFKFRKRMIQKGLNSDFIWVMIGIYTTAKL
GLAKKVTSDLSNLLDRSPITMETFVYDYKELLI

Sequences:

>Translated_273_residues
MILVTGATGNVGKEVVKCLIKRNATFKVTTRSRETEGVYFDFENPSSVKAALTGVTKIFLIRPPHLADAKKYFQPVVDVA
KEIGIKHIVFLSLLGVEKNPIVPHAKIEAMIKASGIPYTFLRPSFFMQNLLSQHGDELRNEKIIEVPAGKGETSFIDVRD
IGEVAAKILTEEGHEFKAYSLTGNEALTYYEVAAIISKIINKDITYTNPSIFKFRKRMIQKGLNSDFIWVMIGIYTTAKL
GLAKKVTSDLSNLLDRSPITMETFVYDYKELLI
>Mature_273_residues
MILVTGATGNVGKEVVKCLIKRNATFKVTTRSRETEGVYFDFENPSSVKAALTGVTKIFLIRPPHLADAKKYFQPVVDVA
KEIGIKHIVFLSLLGVEKNPIVPHAKIEAMIKASGIPYTFLRPSFFMQNLLSQHGDELRNEKIIEVPAGKGETSFIDVRD
IGEVAAKILTEEGHEFKAYSLTGNEALTYYEVAAIISKIINKDITYTNPSIFKFRKRMIQKGLNSDFIWVMIGIYTTAKL
GLAKKVTSDLSNLLDRSPITMETFVYDYKELLI

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790656, Length=237, Percent_Identity=30.379746835443, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 30571; Mature: 30571

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVTGATGNVGKEVVKCLIKRNATFKVTTRSRETEGVYFDFENPSSVKAALTGVTKIFL
CEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCHHHHHHHHHEEEEE
IRPPHLADAKKYFQPVVDVAKEIGIKHIVFLSLLGVEKNPIVPHAKIEAMIKASGIPYTF
ECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEC
LRPSFFMQNLLSQHGDELRNEKIIEVPAGKGETSFIDVRDIGEVAAKILTEEGHEFKAYS
CCHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEE
LTGNEALTYYEVAAIISKIINKDITYTNPSIFKFRKRMIQKGLNSDFIWVMIGIYTTAKL
ECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHH
GLAKKVTSDLSNLLDRSPITMETFVYDYKELLI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MILVTGATGNVGKEVVKCLIKRNATFKVTTRSRETEGVYFDFENPSSVKAALTGVTKIFL
CEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCHHHHHHHHHEEEEE
IRPPHLADAKKYFQPVVDVAKEIGIKHIVFLSLLGVEKNPIVPHAKIEAMIKASGIPYTF
ECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEC
LRPSFFMQNLLSQHGDELRNEKIIEVPAGKGETSFIDVRDIGEVAAKILTEEGHEFKAYS
CCHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEE
LTGNEALTYYEVAAIISKIINKDITYTNPSIFKFRKRMIQKGLNSDFIWVMIGIYTTAKL
ECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHH
GLAKKVTSDLSNLLDRSPITMETFVYDYKELLI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]