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The map label for this gene is gatA2 [H]

Identifier: 218236061

GI number: 218236061

Start: 1835575

End: 1837185

Strand: Direct

Name: gatA2 [H]

Synonym: BCB4264_A1925

Alternate gene names: 218236061

Gene position: 1835575-1837185 (Clockwise)

Preceding gene: 218231421

Following gene: 218235427

Centisome position: 33.87

GC content: 35.63

Gene sequence:

>1611_bases
ATGAAGAAATGGGTAAAGGTAACGCTGTCTATCACTGGAGGCATTGTCTTATTAGCGTGTGCCGGCGGATATTACGTGTA
TAAAAATTATTTTCCAAAGGAACCTGAGCGTATTGTATATGATAAAGAAAGAGTGCTACAACCGATACATAATCAGCTTA
AAGGAATCAATATAGAAAATGTAAAGATAAAAGAAAAAGAAGTCGTAAATGCGACTATAGATGAGCTTCAAAAAATGATT
GATGATGGAAAGTTGTCATATGAAGAATTAACGAGTATTTATCTCTTTAGAATACAAGAACATGATCAAAATGGAATAAC
ATTAAATTCTGTTACAGAGATAAATCCTAATGCGATGGAAGAAGCAAGAAAGTTAGATCAAGAGCGGGGGAGAAACAAAA
ATTCGAATTTATATGGTATTCCTGTCGTTGTAAAAGATAATGTACAGACGGAAAAAGTGATGCCGACTAGTGCAGGAACT
TATGTATTAAAAGATTGGATTGCAGATCAAGACGCAACAATTGTGAAGCAGTTGAAAGAAGAAGGAGCATTTGTTTTAGG
AAAAGCGAATATGTCTGAGTGGGCGAACTATTTATCTTTTACAATGCCAAGCGGGTATAGCGGGAAGAAAGGACAAAATT
TAAATCCGTACGGTCCGATTACATTCGATACTTCAGGATCAAGTTCAGGATCGGCTACAGTAGTTGCAGCAGATTTTGCG
CCGCTTGCAATTGGAACAGAAACGACGGGATCGATTGTTGCACCAGCGGCGCAGCAATCAGTAGTTGGGTTACGTCCATC
TTTAGGTATGGTAAGTAGAACAGGAATTATTCCATTAGCTGAAACTCTTGATACAGCAGGACCTATGGCAAGAACAGTAA
AAGATGCAGCAACGCTATTTAATGTAATGATAGGTTATGATGAAAAAGATGTTATGACAGAAAAAATGAAAGATAAAGAA
AGGATTGATTATACAAAGGATTTATCAATAGATGGATTAAAAGGGAAAAAGATAGGGCTTCTTTTTTCTGTAGACCAACA
AGATGAAAATAGAAAAGCAGTAGCAGAAAAAATTAGAAAAGACCTTCAAGATGCAGGTGCGATATTAACTGATAATATCC
AGTTAAGTGCTGAGGGTGTAGATAATTTGCAAACATTAGAATATGAGTTCAAGCATAATGTAAACGACTATCTTTCACAG
CAAAAGAATGTACCGGTAAAATCGTTAGAAGAGATTATAGCGTTTAATAAAAAGGATAGTAAAAGACGAATAAAATACGG
ACAAACATTAATTGAAGGGTCTGAAAAATCTGCTATAACGAAAGAAGAGTTTGAAAACGTAGTGCAAACGAGTCAAGAAA
ATGCAAGAAAAGAGCTAGATAGGTATTTAGTAGAAAAAGGTTTGGATGCTCTAGTTATGATTAATAACGATGAAGTCCTT
CTATCAGCTGTAGCTGGTTATCCGGAATTAGCGGTTCCTGCTGGATATGATAAAAATGGAGAACCAATCGGTGTAGTGTT
TGTAGGGAAACAGTTTGGTGAAAAAGAGCTTTTCAATATTGGATATGCGTATGAACAGCAGTCTAAAAATAGAAAATCAC
CTAGTTTATAA

Upstream 100 bases:

>100_bases
TCTTTCATTTTTTCATTTAGCATTTTGATTGGAGAGAGTTAAGTAAAAGGTAACTCTCTTTTTTGTATAGCTAAGCATCT
TATAGAGTGGAGGAAGCAAC

Downstream 100 bases:

>100_bases
AAGATTATATGAAAAATAACTGGAATCCACCTAAATTATGTTGGTGGATTCCAGTTAAACGAATCTAACAATATGAAAAG
AAAAAAAGGATTCTTCTCAA

Product: amidase

Products: ADP; phosphate; Gln-tRNA(Gln); L-glutamate

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MKKWVKVTLSITGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGINIENVKIKEKEVVNATIDELQKMI
DDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAMEEARKLDQERGRNKNSNLYGIPVVVKDNVQTEKVMPTSAGT
YVLKDWIADQDATIVKQLKEEGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYGPITFDTSGSSSGSATVVAADFA
PLAIGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLFNVMIGYDEKDVMTEKMKDKE
RIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRKDLQDAGAILTDNIQLSAEGVDNLQTLEYEFKHNVNDYLSQ
QKNVPVKSLEEIIAFNKKDSKRRIKYGQTLIEGSEKSAITKEEFENVVQTSQENARKELDRYLVEKGLDALVMINNDEVL
LSAVAGYPELAVPAGYDKNGEPIGVVFVGKQFGEKELFNIGYAYEQQSKNRKSPSL

Sequences:

>Translated_536_residues
MKKWVKVTLSITGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGINIENVKIKEKEVVNATIDELQKMI
DDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAMEEARKLDQERGRNKNSNLYGIPVVVKDNVQTEKVMPTSAGT
YVLKDWIADQDATIVKQLKEEGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYGPITFDTSGSSSGSATVVAADFA
PLAIGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLFNVMIGYDEKDVMTEKMKDKE
RIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRKDLQDAGAILTDNIQLSAEGVDNLQTLEYEFKHNVNDYLSQ
QKNVPVKSLEEIIAFNKKDSKRRIKYGQTLIEGSEKSAITKEEFENVVQTSQENARKELDRYLVEKGLDALVMINNDEVL
LSAVAGYPELAVPAGYDKNGEPIGVVFVGKQFGEKELFNIGYAYEQQSKNRKSPSL
>Mature_536_residues
MKKWVKVTLSITGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGINIENVKIKEKEVVNATIDELQKMI
DDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAMEEARKLDQERGRNKNSNLYGIPVVVKDNVQTEKVMPTSAGT
YVLKDWIADQDATIVKQLKEEGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYGPITFDTSGSSSGSATVVAADFA
PLAIGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLFNVMIGYDEKDVMTEKMKDKE
RIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRKDLQDAGAILTDNIQLSAEGVDNLQTLEYEFKHNVNDYLSQ
QKNVPVKSLEEIIAFNKKDSKRRIKYGQTLIEGSEKSAITKEEFENVVQTSQENARKELDRYLVEKGLDALVMINNDEVL
LSAVAGYPELAVPAGYDKNGEPIGVVFVGKQFGEKELFNIGYAYEQQSKNRKSPSL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=515, Percent_Identity=24.8543689320388, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI195972892, Length=487, Percent_Identity=26.2833675564682, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=235, Percent_Identity=29.7872340425532, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=511, Percent_Identity=23.679060665362, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=221, Percent_Identity=34.3891402714932, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319685, Length=421, Percent_Identity=25.1781472684086, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648113, Length=506, Percent_Identity=25.6916996047431, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI21356731, Length=277, Percent_Identity=24.187725631769, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24644968, Length=511, Percent_Identity=22.3091976516634, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.7

Molecular weight: Translated: 59461; Mature: 59461

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWVKVTLSITGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGINIEN
CCCEEEEEEEECCCEEEEEECCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCC
VKIKEKEVVNATIDELQKMIDDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAME
EEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEEEECCCCCEEEECEECCCCHHHH
EARKLDQERGRNKNSNLYGIPVVVKDNVQTEKVMPTSAGTYVLKDWIADQDATIVKQLKE
HHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEECCCCCCHHHHHHHHCCCCHHHHHHHHH
EGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYGPITFDTSGSSSGSATVVAADFA
CCCEEEECCCHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEECCC
PLAIGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLF
CEEECCCCCCCEECCCHHHHHHCCCCCCCCHHCCCCCCHHHHHHCCCHHHHHHHHHHHHH
NVMIGYDEKDVMTEKMKDKERIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRK
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
DLQDAGAILTDNIQLSAEGVDNLQTLEYEFKHNVNDYLSQQKNVPVKSLEEIIAFNKKDS
HHHHCCCEEECCEEEECCCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCCCCC
KRRIKYGQTLIEGSEKSAITKEEFENVVQTSQENARKELDRYLVEKGLDALVMINNDEVL
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
LSAVAGYPELAVPAGYDKNGEPIGVVFVGKQFGEKELFNIGYAYEQQSKNRKSPSL
EEHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKWVKVTLSITGGIVLLACAGGYYVYKNYFPKEPERIVYDKERVLQPIHNQLKGINIEN
CCCEEEEEEEECCCEEEEEECCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCC
VKIKEKEVVNATIDELQKMIDDGKLSYEELTSIYLFRIQEHDQNGITLNSVTEINPNAME
EEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEEEECCCCCEEEECEECCCCHHHH
EARKLDQERGRNKNSNLYGIPVVVKDNVQTEKVMPTSAGTYVLKDWIADQDATIVKQLKE
HHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEECCCCCCHHHHHHHHCCCCHHHHHHHHH
EGAFVLGKANMSEWANYLSFTMPSGYSGKKGQNLNPYGPITFDTSGSSSGSATVVAADFA
CCCEEEECCCHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEECCC
PLAIGTETTGSIVAPAAQQSVVGLRPSLGMVSRTGIIPLAETLDTAGPMARTVKDAATLF
CEEECCCCCCCEECCCHHHHHHCCCCCCCCHHCCCCCCHHHHHHCCCHHHHHHHHHHHHH
NVMIGYDEKDVMTEKMKDKERIDYTKDLSIDGLKGKKIGLLFSVDQQDENRKAVAEKIRK
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
DLQDAGAILTDNIQLSAEGVDNLQTLEYEFKHNVNDYLSQQKNVPVKSLEEIIAFNKKDS
HHHHCCCEEECCEEEECCCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCCCCC
KRRIKYGQTLIEGSEKSAITKEEFENVVQTSQENARKELDRYLVEKGLDALVMINNDEVL
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
LSAVAGYPELAVPAGYDKNGEPIGVVFVGKQFGEKELFNIGYAYEQQSKNRKSPSL
EEHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Glu-tRNA(Gln); L-glutamine

Specific reaction: ATP + Glu-tRNA(Gln) + L-glutamine = ADP + phosphate + Gln-tRNA(Gln) + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]