Click here to switch to the map view.

The map label for this gene is dus2 [H]

Identifier: 218235838

GI number: 218235838

Start: 357826

End: 358815

Strand: Direct

Name: dus2 [H]

Synonym: BCB4264_A0382

Alternate gene names: 218235838

Gene position: 357826-358815 (Clockwise)

Preceding gene: 218233353

Following gene: 218235608

Centisome position: 6.6

GC content: 41.52

Gene sequence:

>990_bases
ATGATAGATAATTTTTGGCGTGAATTACCACGACCATTTTTCGTACTTGCACCAATGGAAGATGTGACAGACGTTGTGTT
CCGTCACGTAGTAAGTGAAGCTGGTCGCCCAGACGTATTCTTCACGGAGTTCACAAACTCGGATAGCTATTGTCATCCAG
AGGGTATGAAAAGTGTACGTGGCCGTTTAATTTTTACAGAAGATGAACAACCAATCGTGGCGCATATTTGGGGAGATAAT
CCTGAATATTTCCGTCAAATGAGTATTGGTATGGCAGAGTTAGGATTTAAAGGCATCGATATTAATATGGGTTGCCCTGT
ACCAAACGTAGCATCAAGAGGAAAAGGTAGTGGCCTTATTCTACGTCCAGACGTTGCAGCAGAACTTATCCAAGCAGCAA
AAGCGGGTGGACTACCTGTCAGCGTAAAAACAAGACTTGGCTTTAAAGAGTTAAGCGAATGGGAAGATTGGTTAACGCAC
ATTTTCAAACAAGATATTGCAAACCTTTCTATTCACTTACGTACAAGAGAGGAAATGAGCCAAGTAGATGCGCATTGGGA
ACTAATTCCGGAAATTAAAAAATTACGTGACCGTATCGCACCAAATACGTTAATAACAATCAATGGAGACATCCTTGATC
GTAAAATGGGGCTGGAACTTGCTGAAAAATACGGTATTGATGGCGTAATGATCGGACGAGGCATCTTCAAAAATCCATTT
GCTTTTGAAAAAGAGCCAAGAGAGCATAGCAGTAAAGAGCACCTAGATCTTTTAAGACTACAACTTGATCTTCAAGATCA
ATATGCAGAAGTACTGCCACGCTCAATCACAGGGCTTCACCGCTTCTTCAAAATTTATGTAAAAGGCTTCCCTGGAGCTG
CTGAATTGAGAAATCAATTGATGAGTACGAAATCGACTGATGAGGTGCGTGCTTTGTTGGATAAGTTTGAGGATAGTGTT
GGTGTGGCTCAGGATAGTGAGACGGTTTAA

Upstream 100 bases:

>100_bases
ATTCATAAGCGATTTTAGTTAAAATAAATATTGCTGATTTTCACAAATTATAAATTGAGATTTTGAAATTGCATTCATAG
TGAGAGGATTTGAATTTAAA

Downstream 100 bases:

>100_bases
CTTTTAAGCAGTTAAATCATTTCATATGTAGTGCATGACATGAGTGAGTTGAAAATAAGTGCAGAGCTGGAAAAGAACAG
TGTATTTGAGAGATCAAAAC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MIDNFWRELPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVRGRLIFTEDEQPIVAHIWGDN
PEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLILRPDVAAELIQAAKAGGLPVSVKTRLGFKELSEWEDWLTH
IFKQDIANLSIHLRTREEMSQVDAHWELIPEIKKLRDRIAPNTLITINGDILDRKMGLELAEKYGIDGVMIGRGIFKNPF
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQLMSTKSTDEVRALLDKFEDSV
GVAQDSETV

Sequences:

>Translated_329_residues
MIDNFWRELPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVRGRLIFTEDEQPIVAHIWGDN
PEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLILRPDVAAELIQAAKAGGLPVSVKTRLGFKELSEWEDWLTH
IFKQDIANLSIHLRTREEMSQVDAHWELIPEIKKLRDRIAPNTLITINGDILDRKMGLELAEKYGIDGVMIGRGIFKNPF
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQLMSTKSTDEVRALLDKFEDSV
GVAQDSETV
>Mature_329_residues
MIDNFWRELPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVRGRLIFTEDEQPIVAHIWGDN
PEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLILRPDVAAELIQAAKAGGLPVSVKTRLGFKELSEWEDWLTH
IFKQDIANLSIHLRTREEMSQVDAHWELIPEIKKLRDRIAPNTLITINGDILDRKMGLELAEKYGIDGVMIGRGIFKNPF
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQLMSTKSTDEVRALLDKFEDSV
GVAQDSETV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=315, Percent_Identity=25.0793650793651, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI239788483, Length=265, Percent_Identity=27.9245283018868, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI239788462, Length=174, Percent_Identity=32.183908045977, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788462, Length=240, Percent_Identity=28.75, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789660, Length=238, Percent_Identity=28.9915966386555, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI145693211, Length=252, Percent_Identity=26.5873015873016, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=213, Percent_Identity=27.2300469483568, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=176, Percent_Identity=31.25, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=325, Percent_Identity=25.5384615384615, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921524, Length=236, Percent_Identity=27.5423728813559, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24585320, Length=265, Percent_Identity=26.4150943396226, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37357; Mature: 37357

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDNFWRELPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVR
CCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHC
GRLIFTEDEQPIVAHIWGDNPEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLI
CEEEEECCCCCEEEEEECCCHHHHHHHHCCHHHHCCCCEEEECCCCCCCHHCCCCCCCEE
LRPDVAAELIQAAKAGGLPVSVKTRLGFKELSEWEDWLTHIFKQDIANLSIHLRTREEMS
ECCHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHHHHHHHHHHHHHHCEEEEEECHHHHH
QVDAHWELIPEIKKLRDRIAPNTLITINGDILDRKMGLELAEKYGIDGVMIGRGIFKNPF
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQL
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
MSTKSTDEVRALLDKFEDSVGVAQDSETV
HCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIDNFWRELPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVR
CCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHC
GRLIFTEDEQPIVAHIWGDNPEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLI
CEEEEECCCCCEEEEEECCCHHHHHHHHCCHHHHCCCCEEEECCCCCCCHHCCCCCCCEE
LRPDVAAELIQAAKAGGLPVSVKTRLGFKELSEWEDWLTHIFKQDIANLSIHLRTREEMS
ECCHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHHHHHHHHHHHHHHCEEEEEECHHHHH
QVDAHWELIPEIKKLRDRIAPNTLITINGDILDRKMGLELAEKYGIDGVMIGRGIFKNPF
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQL
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
MSTKSTDEVRALLDKFEDSVGVAQDSETV
HCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]