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The map label for this gene is glcD [H]

Identifier: 218235752

GI number: 218235752

Start: 3393506

End: 3394897

Strand: Reverse

Name: glcD [H]

Synonym: BCB4264_A3515

Alternate gene names: 218235752

Gene position: 3394897-3393506 (Counterclockwise)

Preceding gene: 218235226

Following gene: 218231383

Centisome position: 62.65

GC content: 38.29

Gene sequence:

>1392_bases
ATGGAAATAACAATTGAACATGTAGTAAATGAATTAAAAGGCATACTTCCTGAAGATCGAGTAGTAATAAATATGACAGT
ACGAGAGTTACATAGTAAAGATGAATCTTACCATGTTAGTAGTTTACCTGATGTAGTTGTTTTTCCAAAAACGACCGAAG
AAGTTAGCACGATAATGAAAATAGCGAGTCAGCACGGAACAGCTGTCGTTCCATTTGGAGTAGGGTCCAGTTTAGAGGGA
CATGTAATTCCTTACGAAAAAGGAATTACAATGGACTTTTCTCTTATGAACAAAATACTTGAAATAAGAGAAAAGGATTT
TCTTGTGAGAGTACAGCCAGGGGTGACGCGCTCTCAGCTTAATAAAGAATTGAAAAAGTATGGATTATTTTTTAGTGTAG
ACCCAGGAGCTGATGCAACGTTAGGAGGAATGGCTGCTACAAATGCGAGCGGAACGACAGCGGTAAAGTATGGGGTAATG
CGTGATCAAGTCCGAGATTTAGAAGTCGTTCTTGCTGATGGAGAAGTAATACATACCGGGAATTTAGCGGCGAAGTCATC
ATCAGGTTATCATTTAAATGGTATTTTCGTAGGCTCAGAAGGAACACTTGGATGTTTCACGGAGTTAACTTTAAAAGTAT
ACGGTATACCAGAACATGTTATGGCTGCGAGGGCATCGTTTCCAACTATAAATGATGCAGTAGAAGCTGTAATTAACATA
TTACAGGCAGGTATTCCAATCGCGAGAATTGAACTCGTTGATGAATTATCTATGAAACAAGTAAATCATTACAATGAAAC
AAGTTACAGAGAAGAACCGACACTGTTTTTAGAGTTTCACGGCAATGAAGCAGGGTTAAAGCAAGATATTGAATTTACGA
AAGAAATCGTTCTTGATCATAAATGTATAGAAATTGCGTTTGAGATGGAAACAGCAGCGAGAAATAAACTGTGGGATGCA
AGACATAACTTAGCGTACTCTTACGTGCACAGTTATCCTGGTAAAAAGCTAATGAGTACAGACGTATGTGTGCCAATTTC
GGAATTAGCTGGAGCGATTCAACATGCGAAAGAGGTATTAGATAAAGTAGGGCTTATTGGCGGTATTCTCGGTCATGTTG
GGGATGGGAATTTCCATGTACTCTTAATGATTGATACAAATGATAAAGAGGAAGTGAAGAAGGCAGACGAAATAAATGAA
AGTATTGTTCAGTATGCTCTTAAACGAGGAGGAACGTGTACAGGAGAACATGGCGTTGGAATCGGAAAACGAAAGTATCA
AGAAGAAGAGCATGGGGCAGCATTATTTGTAATGGAGAAAATAAAGAAAGCTCTTGATCCGCAAAATATTTTGAATCCGA
ATAAGGTGTTTCAATTAAAAGATAGGGGCTGA

Upstream 100 bases:

>100_bases
CAGGTTCTTTTTTGTATGTTTAGGTTTAAAAATCTAATTTTTCTAATTATTTTGTATAAAGATGGTATACTTGTTTTAAA
TTTAAAAAGGAGAGTTGATT

Downstream 100 bases:

>100_bases
GTGGATGAAAGTACTAGAAGCGATAAGCAATGTAGCTGGAATGTATTTTGCATTTTGGACCGGCTGCTGCACTCCCAAGT
GTATTAGCAGCAGTATGGCA

Product: putative glycolate oxidase, subunit GlcD

Products: pyruvate; ferrocytochrome c

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MEITIEHVVNELKGILPEDRVVINMTVRELHSKDESYHVSSLPDVVVFPKTTEEVSTIMKIASQHGTAVVPFGVGSSLEG
HVIPYEKGITMDFSLMNKILEIREKDFLVRVQPGVTRSQLNKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVM
RDQVRDLEVVLADGEVIHTGNLAAKSSSGYHLNGIFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPTINDAVEAVINI
LQAGIPIARIELVDELSMKQVNHYNETSYREEPTLFLEFHGNEAGLKQDIEFTKEIVLDHKCIEIAFEMETAARNKLWDA
RHNLAYSYVHSYPGKKLMSTDVCVPISELAGAIQHAKEVLDKVGLIGGILGHVGDGNFHVLLMIDTNDKEEVKKADEINE
SIVQYALKRGGTCTGEHGVGIGKRKYQEEEHGAALFVMEKIKKALDPQNILNPNKVFQLKDRG

Sequences:

>Translated_463_residues
MEITIEHVVNELKGILPEDRVVINMTVRELHSKDESYHVSSLPDVVVFPKTTEEVSTIMKIASQHGTAVVPFGVGSSLEG
HVIPYEKGITMDFSLMNKILEIREKDFLVRVQPGVTRSQLNKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVM
RDQVRDLEVVLADGEVIHTGNLAAKSSSGYHLNGIFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPTINDAVEAVINI
LQAGIPIARIELVDELSMKQVNHYNETSYREEPTLFLEFHGNEAGLKQDIEFTKEIVLDHKCIEIAFEMETAARNKLWDA
RHNLAYSYVHSYPGKKLMSTDVCVPISELAGAIQHAKEVLDKVGLIGGILGHVGDGNFHVLLMIDTNDKEEVKKADEINE
SIVQYALKRGGTCTGEHGVGIGKRKYQEEEHGAALFVMEKIKKALDPQNILNPNKVFQLKDRG
>Mature_463_residues
MEITIEHVVNELKGILPEDRVVINMTVRELHSKDESYHVSSLPDVVVFPKTTEEVSTIMKIASQHGTAVVPFGVGSSLEG
HVIPYEKGITMDFSLMNKILEIREKDFLVRVQPGVTRSQLNKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVM
RDQVRDLEVVLADGEVIHTGNLAAKSSSGYHLNGIFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPTINDAVEAVINI
LQAGIPIARIELVDELSMKQVNHYNETSYREEPTLFLEFHGNEAGLKQDIEFTKEIVLDHKCIEIAFEMETAARNKLWDA
RHNLAYSYVHSYPGKKLMSTDVCVPISELAGAIQHAKEVLDKVGLIGGILGHVGDGNFHVLLMIDTNDKEEVKKADEINE
SIVQYALKRGGTCTGEHGVGIGKRKYQEEEHGAALFVMEKIKKALDPQNILNPNKVFQLKDRG

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=453, Percent_Identity=46.1368653421634, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI37595754, Length=476, Percent_Identity=43.6974789915966, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI119964728, Length=435, Percent_Identity=26.4367816091954, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4501993, Length=219, Percent_Identity=30.1369863013699, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1789351, Length=466, Percent_Identity=29.8283261802575, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1787977, Length=497, Percent_Identity=27.364185110664, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI48994907, Length=492, Percent_Identity=25.2032520325203, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71992373, Length=462, Percent_Identity=43.2900432900433, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71992381, Length=462, Percent_Identity=42.4242424242424, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71992392, Length=361, Percent_Identity=47.0914127423823, Blast_Score=338, Evalue=4e-93,
Organism=Caenorhabditis elegans, GI17534361, Length=450, Percent_Identity=26.4444444444444, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17556096, Length=455, Percent_Identity=26.3736263736264, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6320027, Length=474, Percent_Identity=38.1856540084388, Blast_Score=308, Evalue=9e-85,
Organism=Saccharomyces cerevisiae, GI6320764, Length=477, Percent_Identity=25.5765199161426, Blast_Score=136, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6320023, Length=470, Percent_Identity=24.6808510638298, Blast_Score=126, Evalue=7e-30,
Organism=Drosophila melanogaster, GI18921117, Length=429, Percent_Identity=26.5734265734266, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24639277, Length=429, Percent_Identity=26.5734265734266, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24639275, Length=429, Percent_Identity=26.5734265734266, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24653753, Length=219, Percent_Identity=30.5936073059361, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.2.4

Molecular weight: Translated: 50983; Mature: 50983

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITIEHVVNELKGILPEDRVVINMTVRELHSKDESYHVSSLPDVVVFPKTTEEVSTIMK
CEEEHHHHHHHHHCCCCCCCEEEEEEHHHHHCCCCCEEECCCCCEEEECCCHHHHHHHHH
IASQHGTAVVPFGVGSSLEGHVIPYEKGITMDFSLMNKILEIREKDFLVRVQPGVTRSQL
HHHCCCCEEEEECCCCCCCCCEEECCCCCEEEHHHHHHHHHHHCCCEEEEECCCCCHHHH
NKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVMRDQVRDLEVVLADGEVIHTG
HHHHHHHCCEEEECCCCCCCCCCEEECCCCCCHHEEHHHHHHHHCCEEEEEECCCEEECC
NLAAKSSSGYHLNGIFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPTINDAVEAVINI
CCEECCCCCEEEEEEEECCCCCEEEHEEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHH
LQAGIPIARIELVDELSMKQVNHYNETSYREEPTLFLEFHGNEAGLKQDIEFTKEIVLDH
HHCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
KCIEIAFEMETAARNKLWDARHNLAYSYVHSYPGKKLMSTDVCVPISELAGAIQHAKEVL
HHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHH
DKVGLIGGILGHVGDGNFHVLLMIDTNDKEEVKKADEINESIVQYALKRGGTCTGEHGVG
HHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
IGKRKYQEEEHGAALFVMEKIKKALDPQNILNPNKVFQLKDRG
CCHHHCCHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCC
>Mature Secondary Structure
MEITIEHVVNELKGILPEDRVVINMTVRELHSKDESYHVSSLPDVVVFPKTTEEVSTIMK
CEEEHHHHHHHHHCCCCCCCEEEEEEHHHHHCCCCCEEECCCCCEEEECCCHHHHHHHHH
IASQHGTAVVPFGVGSSLEGHVIPYEKGITMDFSLMNKILEIREKDFLVRVQPGVTRSQL
HHHCCCCEEEEECCCCCCCCCEEECCCCCEEEHHHHHHHHHHHCCCEEEEECCCCCHHHH
NKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVMRDQVRDLEVVLADGEVIHTG
HHHHHHHCCEEEECCCCCCCCCCEEECCCCCCHHEEHHHHHHHHCCEEEEEECCCEEECC
NLAAKSSSGYHLNGIFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPTINDAVEAVINI
CCEECCCCCEEEEEEEECCCCCEEEHEEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHH
LQAGIPIARIELVDELSMKQVNHYNETSYREEPTLFLEFHGNEAGLKQDIEFTKEIVLDH
HHCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
KCIEIAFEMETAARNKLWDARHNLAYSYVHSYPGKKLMSTDVCVPISELAGAIQHAKEVL
HHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHH
DKVGLIGGILGHVGDGNFHVLLMIDTNDKEEVKKADEINESIVQYALKRGGTCTGEHGVG
HHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
IGKRKYQEEEHGAALFVMEKIKKALDPQNILNPNKVFQLKDRG
CCHHHCCHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; ferricytochrome c

Specific reaction: (R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]