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The map label for this gene is ywjE [H]

Identifier: 218235726

GI number: 218235726

Start: 1711454

End: 1712716

Strand: Direct

Name: ywjE [H]

Synonym: BCB4264_A1807

Alternate gene names: 218235726

Gene position: 1711454-1712716 (Clockwise)

Preceding gene: 218235154

Following gene: 218232371

Centisome position: 31.58

GC content: 33.49

Gene sequence:

>1263_bases
TTGAATGACAAAATAGAAAGGTGGAAAGAAAATGATCGGAAGGAAATCAAAATGATTAAAAAAATATTGCGAGTTACTTC
TATTGTTATTGCTATTTTCGTTTTTATTTTAATATGGATGCATATCGATGTTACCTTAGGTAGGAAAATGGAAGCTGGGA
AAAACATGCCGACAGAGTATGCGCCTCATTATAGTGACTTTCAATTATATGTAGAAGGGAAGTCTTTTTATAAACAATTA
TTTACTGATATAAGAGAAGCCAAAAGCTCCATTTACACGTATTTCTTCATTTTGTCGGATGATAAAAGTAGTCATACTTT
TTTAAACTTATTAAAAGAGAAGGCAAAAGAAGGAGTAAACGTCTATTTATCAGTTGATCGCATTAATGATTTATCATTTG
AAAGAAAGGTGATAAATGAATTGCGGAAAAGTGGTGTACATTTTACATATAGTAGAAAACCTACATTGCCATTCGGATTT
TATTCACTTCACCATCGTAATCATCGCCGTATTACGACGATTGATGGGGAGATTGGCTATACAGGCGGATTTAATATAGG
AGATGAGTACTTAGGGAAAGATAAGCGATTTGGATATTGGCGTGATTATCATGTACGACTTAAAGGAGAAGGAGCAAAAG
ATTTAGAACAGCAATTCGCTTTAGACTGGAAACGAGATACGAAAGAAGAGATAAAGAGAAGTACGAATAAAGCTAGTAAA
GGGAATACATTACATACTATGACTAGTTACAATGGGCATTACGTTGCTGAAAAATATATAGAGCTCATAAAACAAGCTCA
GCACTCCATTGTAATTGCAACGCCGTATTTTATAGCAAAAAATAAAGAATCCATGAATGCTTTAATCGCAGCACAAAAGC
GTGGTGTTACAGTAAAAATACTTTGGTCATATAAACCAGATCTACCTCTTATAAAAGAAGCGGCGTATCCATACATACGT
CAAGCTGTTAATAATGGGATTACTGTATATGGGTATAAAAAAGGAATGTTCCATGGCAAATTAATGCTTATTGATAATGA
ATTAACCGTTATTGGTACAACAAACTTTACTTCGCGTAGCTTCAATATAAATGATGAAATGAATTTTTATATTTATGGTG
GTCCTATTGTAGGACAAGTTAATAAGGCGTTAACAGAGGATTTTCATGATTCGAAAGAAATGACGAAAGAGTTTTTTGAA
AAGTTATCTTTTTGGGAGCGTTGTAAGGAGAAAGTGGCGGGATTGGTTGACTTCTATTTGTGA

Upstream 100 bases:

>100_bases
ATAAGTTTGGAAGAAATAATGATGCGAGGAATTTTGTTTAATGTGAAAAGGATGTGACGTTAAGTCACATCCTTTTTTGT
ATGAAGCATGAAATCTAAAT

Downstream 100 bases:

>100_bases
TGATGAATTAGGAAAGGGTGCCTACAAAAGACACCCTTTTTTGGTGGGAAATCATTATTTTTCATGCACCAACATCGTAA
CATCATCATTAATCAATGTC

Product: cardiolipin synthetase domain protein

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MNDKIERWKENDRKEIKMIKKILRVTSIVIAIFVFILIWMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKSFYKQL
FTDIREAKSSIYTYFFILSDDKSSHTFLNLLKEKAKEGVNVYLSVDRINDLSFERKVINELRKSGVHFTYSRKPTLPFGF
YSLHHRNHRRITTIDGEIGYTGGFNIGDEYLGKDKRFGYWRDYHVRLKGEGAKDLEQQFALDWKRDTKEEIKRSTNKASK
GNTLHTMTSYNGHYVAEKYIELIKQAQHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAAYPYIR
QAVNNGITVYGYKKGMFHGKLMLIDNELTVIGTTNFTSRSFNINDEMNFYIYGGPIVGQVNKALTEDFHDSKEMTKEFFE
KLSFWERCKEKVAGLVDFYL

Sequences:

>Translated_420_residues
MNDKIERWKENDRKEIKMIKKILRVTSIVIAIFVFILIWMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKSFYKQL
FTDIREAKSSIYTYFFILSDDKSSHTFLNLLKEKAKEGVNVYLSVDRINDLSFERKVINELRKSGVHFTYSRKPTLPFGF
YSLHHRNHRRITTIDGEIGYTGGFNIGDEYLGKDKRFGYWRDYHVRLKGEGAKDLEQQFALDWKRDTKEEIKRSTNKASK
GNTLHTMTSYNGHYVAEKYIELIKQAQHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAAYPYIR
QAVNNGITVYGYKKGMFHGKLMLIDNELTVIGTTNFTSRSFNINDEMNFYIYGGPIVGQVNKALTEDFHDSKEMTKEFFE
KLSFWERCKEKVAGLVDFYL
>Mature_420_residues
MNDKIERWKENDRKEIKMIKKILRVTSIVIAIFVFILIWMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKSFYKQL
FTDIREAKSSIYTYFFILSDDKSSHTFLNLLKEKAKEGVNVYLSVDRINDLSFERKVINELRKSGVHFTYSRKPTLPFGF
YSLHHRNHRRITTIDGEIGYTGGFNIGDEYLGKDKRFGYWRDYHVRLKGEGAKDLEQQFALDWKRDTKEEIKRSTNKASK
GNTLHTMTSYNGHYVAEKYIELIKQAQHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAAYPYIR
QAVNNGITVYGYKKGMFHGKLMLIDNELTVIGTTNFTSRSFNINDEMNFYIYGGPIVGQVNKALTEDFHDSKEMTKEFFE
KLSFWERCKEKVAGLVDFYL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=364, Percent_Identity=25.8241758241758, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1787007, Length=370, Percent_Identity=26.7567567567568, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 49099; Mature: 49099

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKIERWKENDRKEIKMIKKILRVTSIVIAIFVFILIWMHIDVTLGRKMEAGKNMPTEY
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCC
APHYSDFQLYVEGKSFYKQLFTDIREAKSSIYTYFFILSDDKSSHTFLNLLKEKAKEGVN
CCCCCCEEEEECCHHHHHHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHCCCCE
VYLSVDRINDLSFERKVINELRKSGVHFTYSRKPTLPFGFYSLHHRNHRRITTIDGEIGY
EEEEEECCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHCCCCCEEEEECCCCCC
TGGFNIGDEYLGKDKRFGYWRDYHVRLKGEGAKDLEQQFALDWKRDTKEEIKRSTNKASK
CCCCCCCHHHCCCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCC
GNTLHTMTSYNGHYVAEKYIELIKQAQHSIVIATPYFIAKNKESMNALIAAQKRGVTVKI
CCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCEEEECCCHHHHHHHHHHCCCCEEEE
LWSYKPDLPLIKEAAYPYIRQAVNNGITVYGYKKGMFHGKLMLIDNELTVIGTTNFTSRS
EEECCCCCCHHHHHCCHHHHHHHHCCEEEEEEECCCCCCEEEEEECCEEEEECCCCCCCE
FNINDEMNFYIYGGPIVGQVNKALTEDFHDSKEMTKEFFEKLSFWERCKEKVAGLVDFYL
ECCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNDKIERWKENDRKEIKMIKKILRVTSIVIAIFVFILIWMHIDVTLGRKMEAGKNMPTEY
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCC
APHYSDFQLYVEGKSFYKQLFTDIREAKSSIYTYFFILSDDKSSHTFLNLLKEKAKEGVN
CCCCCCEEEEECCHHHHHHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHCCCCE
VYLSVDRINDLSFERKVINELRKSGVHFTYSRKPTLPFGFYSLHHRNHRRITTIDGEIGY
EEEEEECCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHCCCCCEEEEECCCCCC
TGGFNIGDEYLGKDKRFGYWRDYHVRLKGEGAKDLEQQFALDWKRDTKEEIKRSTNKASK
CCCCCCCHHHCCCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCC
GNTLHTMTSYNGHYVAEKYIELIKQAQHSIVIATPYFIAKNKESMNALIAAQKRGVTVKI
CCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCEEEECCCHHHHHHHHHHCCCCEEEE
LWSYKPDLPLIKEAAYPYIRQAVNNGITVYGYKKGMFHGKLMLIDNELTVIGTTNFTSRS
EEECCCCCCHHHHHCCHHHHHHHHCCEEEEEEECCCCCCEEEEEECCEEEEECCCCCCCE
FNINDEMNFYIYGGPIVGQVNKALTEDFHDSKEMTKEFFEKLSFWERCKEKVAGLVDFYL
ECCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]