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The map label for this gene is bmr3 [H]

Identifier: 218235721

GI number: 218235721

Start: 2775921

End: 2777456

Strand: Reverse

Name: bmr3 [H]

Synonym: BCB4264_A2894

Alternate gene names: 218235721

Gene position: 2777456-2775921 (Counterclockwise)

Preceding gene: 218231422

Following gene: 218234763

Centisome position: 51.25

GC content: 37.96

Gene sequence:

>1536_bases
ATGGTTGAGAAGAATAATAAGCTCGGCTTTGTTGTGGCGGGCTTATTGCTAGGTATTTTAATGGCATCGATGGATAATAC
CATTGTCGTAACGGCGATGGGAACAATCGTTGGTGACTTAGGAGGCCTCGAAAACTTTGTATGGGTCGTTTCTGCCTATA
TGGTCGCAGAAATGGCAGGCATGCCGATTTTCGGTAAGCTATCAGATATGTATGGTAGAAAGAGATTCTTTATTTTCGGT
TTAATTGTCTTTATGATTGGTTCGGCACTTTGTGGTACTGCTGAAAATATTACACAGTTAGGTATTTATCGTGCCATTCA
AGGTATTGGCGGCGGGGCATTAGTGCCAATCGCGTTTACTATCGTTTTTGATATTTTCCCACCTGAAAAACGCGGGAAAA
TGGGTGGTTTATTCGGAGCGGTATTTGGATTATCTAGTATTTTCGGGCCATTACTTGGCGCATATATTACCGACTACATT
AGCTGGCACTGGGTATTTTATATTAACTTACCACTTGGCATTTTAGCACTTATTTTTATTACATTCTTTTATAAAGAATC
ACGCGTTCATAGGAAGCAAAAGATTGATTGGTTTGGTGCAATTACTTTAGTTGGTGCAGTAGTTTCTTTAATGTTTGCGC
TTGAACTAGGCGGACAAAAATATGATTGGGATTCTAGCTTTATTTTAAGCTTATTTGGTGGATTCGCTATTTTAATCATC
GCATTCATTTTTATTGAACGAAAAGTAGAAGAACCAATTATTTCATTTGAGATGTTTAAGCAGCGCTTATTCGGAATGAG
TACAATTATTGCTTTATGTTACGGTGCTGCATTTATGTCAGCAACTGTGTACATTCCGTTATTTATTCAAGGTGTATACG
GTGGTACCGCAACAAACTCAGGATTATTACTTTTACCGATGATGTTAGGATCAGTCGTAACAGCGCAGTTAGGCGGATTT
TTAACAACTAAACTTAGCTACCGAAACATTATGATTATTTCTGCGGTTATTATGCTAATTGGACTATTCTTATTAAGCAC
GTTAACACCAGAAACAAGTCGTGCATTATTAACAGTTTATATGATTATTATCGGATTTGGAGTCGGTTTCTCATTCTCTG
TACTAAGTATGGCAGCTATTCACAACTTCGGTATGGAACAGCGCGGGTCTGCGACTTCAACGAGTAACTTCATTCGTTCG
TTAGGAATGACACTAGGCATTACAATCTTCGGAATGATCCAAAGAACAGGTTTCCAAGATCAATTAGAAGAAGCATTTAA
AGGGATGAGCGGGGGAATGAATACAAACGCTTTAGGGGATTCAAGAGCTATTCTATCAGAATCGGCAAGATCTCAAATCC
CTCCGCAAATATTAGATAAGATTATTGACGCTCTTTCTAGTTCAATTGTTCAAACATTCATGTGGGCATTGGTACCAGCT
GGATTAGCATTCATATTCATTTTCTTTATGGGAAATGAGCGCATGGTAATTAAGAAACAACAAACGAATAAAAAGAGTGA
AACATCAAAAGCATAA

Upstream 100 bases:

>100_bases
AATTAGTGTTGTAGAATATCATACTAGTTTTAATCAAAAAGGTAATGCAGTCGTAAAAGAACATAAACAAATATAAGTAG
TATGAGGGAGAGCAAGAGAT

Downstream 100 bases:

>100_bases
TGAAAAAGCTCCTTTCTAATTGGAAAGGAGCTTTTTCGTATGTACGGATTTAGATTAGCCTTCGATAATTTCGTATTCTT
CGTCTGCTAATAGTAAAATT

Product: drug resistance transporter, EmrB/QacA family

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG
LIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYI
SWHWVFYINLPLGILALIFITFFYKESRVHRKQKIDWFGAITLVGAVVSLMFALELGGQKYDWDSSFILSLFGGFAILII
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGTATNSGLLLLPMMLGSVVTAQLGGF
LTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVYMIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRS
LGMTLGITIFGMIQRTGFQDQLEEAFKGMSGGMNTNALGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
GLAFIFIFFMGNERMVIKKQQTNKKSETSKA

Sequences:

>Translated_511_residues
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG
LIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYI
SWHWVFYINLPLGILALIFITFFYKESRVHRKQKIDWFGAITLVGAVVSLMFALELGGQKYDWDSSFILSLFGGFAILII
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGTATNSGLLLLPMMLGSVVTAQLGGF
LTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVYMIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRS
LGMTLGITIFGMIQRTGFQDQLEEAFKGMSGGMNTNALGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
GLAFIFIFFMGNERMVIKKQQTNKKSETSKA
>Mature_511_residues
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG
LIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYI
SWHWVFYINLPLGILALIFITFFYKESRVHRKQKIDWFGAITLVGAVVSLMFALELGGQKYDWDSSFILSLFGGFAILII
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGTATNSGLLLLPMMLGSVVTAQLGGF
LTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVYMIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRS
LGMTLGITIFGMIQRTGFQDQLEEAFKGMSGGMNTNALGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
GLAFIFIFFMGNERMVIKKQQTNKKSETSKA

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=403, Percent_Identity=30.7692307692308, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI87081983, Length=404, Percent_Identity=28.960396039604, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1788710, Length=441, Percent_Identity=28.3446712018141, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1790195, Length=421, Percent_Identity=25.1781472684086, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1788392, Length=412, Percent_Identity=25.2427184466019, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1790146, Length=162, Percent_Identity=30.2469135802469, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI48994889, Length=134, Percent_Identity=30.5970149253731, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=554, Percent_Identity=23.6462093862816, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319770, Length=478, Percent_Identity=25.3138075313808, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6319783, Length=461, Percent_Identity=24.295010845987, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6323735, Length=324, Percent_Identity=30.2469135802469, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6325455, Length=529, Percent_Identity=23.2514177693762, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321663, Length=540, Percent_Identity=22.4074074074074, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324954, Length=401, Percent_Identity=24.4389027431421, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323520, Length=410, Percent_Identity=24.8780487804878, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55714; Mature: 55714

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAG
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHC
MPIFGKLSDMYGRKRFFIFGLIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFT
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
IVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYISWHWVFYINLPLGILALIFI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHH
TFFYKESRVHRKQKIDWFGAITLVGAVVSLMFALELGGQKYDWDSSFILSLFGGFAILII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGTATNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
GLLLLPMMLGSVVTAQLGGFLTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVY
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRSLGMTLGITIFGMIQRTGFQD
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QLEEAFKGMSGGMNTNALGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAFIFIFFMGNERMVIKKQQTNKKSETSKA
HHHHHHHHHCCCCEEEEEEHHCCCCCCCCCC
>Mature Secondary Structure
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAG
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHC
MPIFGKLSDMYGRKRFFIFGLIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFT
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
IVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYISWHWVFYINLPLGILALIFI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHH
TFFYKESRVHRKQKIDWFGAITLVGAVVSLMFALELGGQKYDWDSSFILSLFGGFAILII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGTATNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
GLLLLPMMLGSVVTAQLGGFLTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVY
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRSLGMTLGITIFGMIQRTGFQD
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QLEEAFKGMSGGMNTNALGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAFIFIFFMGNERMVIKKQQTNKKSETSKA
HHHHHHHHHCCCCEEEEEEHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]