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The map label for this gene is topB [H]

Identifier: 218235662

GI number: 218235662

Start: 412608

End: 414797

Strand: Direct

Name: topB [H]

Synonym: BCB4264_A0431

Alternate gene names: 218235662

Gene position: 412608-414797 (Clockwise)

Preceding gene: 218234970

Following gene: 218234107

Centisome position: 7.61

GC content: 36.12

Gene sequence:

>2190_bases
ATGGCAAAGAGTGTAGTAATTGCTGAGAAACCATCAGTTGCACGTGATATAGCAAAAGTATTAAAGTGTACAAAAAAAGG
TAATGGATTTTTAGAAGGTGACAAATACATCGTGACTTGGGCTCTTGGGCATTTAGTGACGCTTGCAGATCCTGAGGCTT
ATGATAATAAGTATAAAACATGGAATCTTGAAGATCTTCCAATGCTTCCAGAACGTATGAAATTAGTTGTTATAAAGCAA
ACGGGAAAACAGTTTAATGCTGTGAAACATCAACTTACTCGAAATGATGTTAATGAGGTAATTGTGGCTACCGATGCAGG
AAGAGAGGGCGAGTTAGTCGCACGTTGGATAATTGAAAAAGCAAAAGTAAATAAACCAATTAAGCGTTTATGGATTTCTT
CTGTAACAGACAAAGCAATTAAAGATGGCTTTAATAATTTGAAACCAGGTAAAGCTTATGAAAATTTATACGCTTCAGCA
GTTGCACGTTCAGAAGCAGATTGGTACATCGGTTTAAATGCCACTCGTGCACTAACAACAAAGTTTAATGCTCAATTAAA
CTGTGGTCGTGTTCAAACACCTACTGTCGCTATGATCGCTAGTCGTGAAGATGAAATAAAGAACTTCAAAGCTCAAACTT
ACTACGGCATCGAAGCTCAAACAACCGATAGGTTAAAACTAACTTGGCAAGATGCAAACGGTAATAGCCGTAGTTTTAAT
AAAGAAAAAATTGATGGTATTGTAAAAGGTTTAGATAAACATAATGCTACTGTTGTAGAAATTGATAAAAAACAGAAGAA
GTCATTCTCTCCTGGTCTTTACGATTTAACTGAATTGCAACGTGATGCAAATAAAAAGTTCGGTTACTCTGCGAAAGAGA
CATTAAACATTATGCAGAAACTGTATGAGCAACATAAAGTGCTAACGTACCCTCGTACAGATTCACGCTACATTTCATCT
GATATCGTTGGAACACTTCCAGAACGTTTAAAAGCATGTGGTGTTGGAGAATATCGTCCCTTCGCACATAAAGTATTACA
AAAGCCTATCAAACCTAACAAATCATTTGTTGATGATAGTAAAGTAAGCGACCATCATGCGATTATTCCAACAGAAGGAT
ACGTTAACTTCTCAGCCTTCACAGATAAAGAACGTAAAATTTATGATTTAGTTGTAAAGCGTTTCTTAGCTGTTTTATTC
CCAGCCTTCGAATACGAGCAACTAACGTTACGTACAAAAGTCAGTAATGAAACGTTCGTTGCACGCGGAAAAACAATTTT
ACATGCTGGTTGGAAGGAAGTATACGAAAATCGATTTGAAGACGATGATGTAACTGATGACGTAAAAGAGCAGCTTCTAC
CTCACATTGAAAAGGGCGATACATTAACTGTAAAGTTACTTATGCAAACATCAGGTCAAACAAAAGCACCTGCACGATTT
AACGAAGCGACTTTACTTTCAGCAATGGAAAATCCTACAAAATATATGGATACGCAAAATAAACAACTTGCTGATACGTT
AAAATCGACTGGTGGATTAGGTACTGTAGCGACACGTGCAGACATTATCGACAAGCTATTCAATTCATTCTTAATTGAAA
AACGTGGTAAAGATATTCACATTACTTCAAAAGGACGTCAATTACTTGATTTAGTGCCAGAAGAGTTAAAATCACCTACA
CTAACTGGTGAGTGGGAACAAAAGCTCGAAGCAATTGCAAAAGGAAAACTAAAGAAAGAAGTATTCATTTCGGAAATGAA
GAACTATACGAAAGAAATTGTTTCTGAAATTAAATCGAGTGATAAAAAATATAAACATGACAACATTTCAACGAAGTCTT
GTCCAGATTGCGGTAAACCAATGCTAGAAGTAAACGGCAAAAAGGGAAAAATGCTCGTATGCCAAGATCGTGAATGTGGT
CATCGTAAAAATGTATCTCGTACAACAAACGCTCGCTGTCCGCAATGTAAGAAGAAACTAGAATTACGTGGTGAGGGTGC
AGGCCAAATTTTCGCATGTAAATGTGGCTATCGTGAGAAATTATCAACATTCCAAGAACGACGTAAAAAAGAATCTGGAA
ACAAAGCCGATAAACGCGACGTTCAAAAATATATGAAACAACAGAAAAAAGAAGAAGAACCATTAAATAACCCATTCGCA
GAAGCATTAAAAAAATTAAAATTTGATTAA

Upstream 100 bases:

>100_bases
GTTATGTCAATTATGGCTGATATATAAGGTGATCCTTACTGCTCTTCTTGAAAACTGTGGTATATTAAACGGTATATAGT
TAGTAAAGGAGTTTGAATAA

Downstream 100 bases:

>100_bases
ATAAAAAGAATTGTCCATCTAAAGTTTTGATGTGCAATTCTTTTTTATATGTACCATGTTAAACTATTCTAAATTGTAAA
ATTTAAATGATACACATTCC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 729; Mature: 728

Protein sequence:

>729_residues
MAKSVVIAEKPSVARDIAKVLKCTKKGNGFLEGDKYIVTWALGHLVTLADPEAYDNKYKTWNLEDLPMLPERMKLVVIKQ
TGKQFNAVKHQLTRNDVNEVIVATDAGREGELVARWIIEKAKVNKPIKRLWISSVTDKAIKDGFNNLKPGKAYENLYASA
VARSEADWYIGLNATRALTTKFNAQLNCGRVQTPTVAMIASREDEIKNFKAQTYYGIEAQTTDRLKLTWQDANGNSRSFN
KEKIDGIVKGLDKHNATVVEIDKKQKKSFSPGLYDLTELQRDANKKFGYSAKETLNIMQKLYEQHKVLTYPRTDSRYISS
DIVGTLPERLKACGVGEYRPFAHKVLQKPIKPNKSFVDDSKVSDHHAIIPTEGYVNFSAFTDKERKIYDLVVKRFLAVLF
PAFEYEQLTLRTKVSNETFVARGKTILHAGWKEVYENRFEDDDVTDDVKEQLLPHIEKGDTLTVKLLMQTSGQTKAPARF
NEATLLSAMENPTKYMDTQNKQLADTLKSTGGLGTVATRADIIDKLFNSFLIEKRGKDIHITSKGRQLLDLVPEELKSPT
LTGEWEQKLEAIAKGKLKKEVFISEMKNYTKEIVSEIKSSDKKYKHDNISTKSCPDCGKPMLEVNGKKGKMLVCQDRECG
HRKNVSRTTNARCPQCKKKLELRGEGAGQIFACKCGYREKLSTFQERRKKESGNKADKRDVQKYMKQQKKEEEPLNNPFA
EALKKLKFD

Sequences:

>Translated_729_residues
MAKSVVIAEKPSVARDIAKVLKCTKKGNGFLEGDKYIVTWALGHLVTLADPEAYDNKYKTWNLEDLPMLPERMKLVVIKQ
TGKQFNAVKHQLTRNDVNEVIVATDAGREGELVARWIIEKAKVNKPIKRLWISSVTDKAIKDGFNNLKPGKAYENLYASA
VARSEADWYIGLNATRALTTKFNAQLNCGRVQTPTVAMIASREDEIKNFKAQTYYGIEAQTTDRLKLTWQDANGNSRSFN
KEKIDGIVKGLDKHNATVVEIDKKQKKSFSPGLYDLTELQRDANKKFGYSAKETLNIMQKLYEQHKVLTYPRTDSRYISS
DIVGTLPERLKACGVGEYRPFAHKVLQKPIKPNKSFVDDSKVSDHHAIIPTEGYVNFSAFTDKERKIYDLVVKRFLAVLF
PAFEYEQLTLRTKVSNETFVARGKTILHAGWKEVYENRFEDDDVTDDVKEQLLPHIEKGDTLTVKLLMQTSGQTKAPARF
NEATLLSAMENPTKYMDTQNKQLADTLKSTGGLGTVATRADIIDKLFNSFLIEKRGKDIHITSKGRQLLDLVPEELKSPT
LTGEWEQKLEAIAKGKLKKEVFISEMKNYTKEIVSEIKSSDKKYKHDNISTKSCPDCGKPMLEVNGKKGKMLVCQDRECG
HRKNVSRTTNARCPQCKKKLELRGEGAGQIFACKCGYREKLSTFQERRKKESGNKADKRDVQKYMKQQKKEEEPLNNPFA
EALKKLKFD
>Mature_728_residues
AKSVVIAEKPSVARDIAKVLKCTKKGNGFLEGDKYIVTWALGHLVTLADPEAYDNKYKTWNLEDLPMLPERMKLVVIKQT
GKQFNAVKHQLTRNDVNEVIVATDAGREGELVARWIIEKAKVNKPIKRLWISSVTDKAIKDGFNNLKPGKAYENLYASAV
ARSEADWYIGLNATRALTTKFNAQLNCGRVQTPTVAMIASREDEIKNFKAQTYYGIEAQTTDRLKLTWQDANGNSRSFNK
EKIDGIVKGLDKHNATVVEIDKKQKKSFSPGLYDLTELQRDANKKFGYSAKETLNIMQKLYEQHKVLTYPRTDSRYISSD
IVGTLPERLKACGVGEYRPFAHKVLQKPIKPNKSFVDDSKVSDHHAIIPTEGYVNFSAFTDKERKIYDLVVKRFLAVLFP
AFEYEQLTLRTKVSNETFVARGKTILHAGWKEVYENRFEDDDVTDDVKEQLLPHIEKGDTLTVKLLMQTSGQTKAPARFN
EATLLSAMENPTKYMDTQNKQLADTLKSTGGLGTVATRADIIDKLFNSFLIEKRGKDIHITSKGRQLLDLVPEELKSPTL
TGEWEQKLEAIAKGKLKKEVFISEMKNYTKEIVSEIKSSDKKYKHDNISTKSCPDCGKPMLEVNGKKGKMLVCQDRECGH
RKNVSRTTNARCPQCKKKLELRGEGAGQIFACKCGYREKLSTFQERRKKESGNKADKRDVQKYMKQQKKEEEPLNNPFAE
ALKKLKFD

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=642, Percent_Identity=24.2990654205607, Blast_Score=133, Evalue=6e-31,
Organism=Homo sapiens, GI4507635, Length=626, Percent_Identity=24.9201277955272, Blast_Score=129, Evalue=1e-29,
Organism=Escherichia coli, GI1788061, Length=620, Percent_Identity=32.5806451612903, Blast_Score=340, Evalue=3e-94,
Organism=Escherichia coli, GI1787529, Length=674, Percent_Identity=25.9643916913947, Blast_Score=163, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI32563869, Length=746, Percent_Identity=24.798927613941, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=640, Percent_Identity=25.3125, Blast_Score=122, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6323263, Length=700, Percent_Identity=22, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24585251, Length=592, Percent_Identity=25.1689189189189, Blast_Score=133, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24640096, Length=617, Percent_Identity=23.9870340356564, Blast_Score=113, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 82799; Mature: 82668

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSVVIAEKPSVARDIAKVLKCTKKGNGFLEGDKYIVTWALGHLVTLADPEAYDNKYKT
CCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHEEEECCCHHHCCCCCC
WNLEDLPMLPERMKLVVIKQTGKQFNAVKHQLTRNDVNEVIVATDAGREGELVARWIIEK
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHH
AKVNKPIKRLWISSVTDKAIKDGFNNLKPGKAYENLYASAVARSEADWYIGLNATRALTT
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHE
KFNAQLNCGRVQTPTVAMIASREDEIKNFKAQTYYGIEAQTTDRLKLTWQDANGNSRSFN
ECCCEECCCCCCCCEEEEEECCHHHHHCCCCCEEECCEECCCCEEEEEEECCCCCCCCCC
KEKIDGIVKGLDKHNATVVEIDKKQKKSFSPGLYDLTELQRDANKKFGYSAKETLNIMQK
HHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
LYEQHKVLTYPRTDSRYISSDIVGTLPERLKACGVGEYRPFAHKVLQKPIKPNKSFVDDS
HHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCC
KVSDHHAIIPTEGYVNFSAFTDKERKIYDLVVKRFLAVLFPAFEYEQLTLRTKVSNETFV
CCCCCCEEECCCCCEEEHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
ARGKTILHAGWKEVYENRFEDDDVTDDVKEQLLPHIEKGDTLTVKLLMQTSGQTKAPARF
ECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCC
NEATLLSAMENPTKYMDTQNKQLADTLKSTGGLGTVATRADIIDKLFNSFLIEKRGKDIH
CHHHHHHHHCCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
ITSKGRQLLDLVPEELKSPTLTGEWEQKLEAIAKGKLKKEVFISEMKNYTKEIVSEIKSS
EECCCHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DKKYKHDNISTKSCPDCGKPMLEVNGKKGKMLVCQDRECGHRKNVSRTTNARCPQCKKKL
CCHHCCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
ELRGEGAGQIFACKCGYREKLSTFQERRKKESGNKADKRDVQKYMKQQKKEEEPLNNPFA
HCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHH
EALKKLKFD
HHHHHHCCC
>Mature Secondary Structure 
AKSVVIAEKPSVARDIAKVLKCTKKGNGFLEGDKYIVTWALGHLVTLADPEAYDNKYKT
CCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHEEEECCCHHHCCCCCC
WNLEDLPMLPERMKLVVIKQTGKQFNAVKHQLTRNDVNEVIVATDAGREGELVARWIIEK
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHH
AKVNKPIKRLWISSVTDKAIKDGFNNLKPGKAYENLYASAVARSEADWYIGLNATRALTT
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHE
KFNAQLNCGRVQTPTVAMIASREDEIKNFKAQTYYGIEAQTTDRLKLTWQDANGNSRSFN
ECCCEECCCCCCCCEEEEEECCHHHHHCCCCCEEECCEECCCCEEEEEEECCCCCCCCCC
KEKIDGIVKGLDKHNATVVEIDKKQKKSFSPGLYDLTELQRDANKKFGYSAKETLNIMQK
HHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
LYEQHKVLTYPRTDSRYISSDIVGTLPERLKACGVGEYRPFAHKVLQKPIKPNKSFVDDS
HHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCC
KVSDHHAIIPTEGYVNFSAFTDKERKIYDLVVKRFLAVLFPAFEYEQLTLRTKVSNETFV
CCCCCCEEECCCCCEEEHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
ARGKTILHAGWKEVYENRFEDDDVTDDVKEQLLPHIEKGDTLTVKLLMQTSGQTKAPARF
ECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCC
NEATLLSAMENPTKYMDTQNKQLADTLKSTGGLGTVATRADIIDKLFNSFLIEKRGKDIH
CHHHHHHHHCCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
ITSKGRQLLDLVPEELKSPTLTGEWEQKLEAIAKGKLKKEVFISEMKNYTKEIVSEIKSS
EECCCHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DKKYKHDNISTKSCPDCGKPMLEVNGKKGKMLVCQDRECGHRKNVSRTTNARCPQCKKKL
CCHHCCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
ELRGEGAGQIFACKCGYREKLSTFQERRKKESGNKADKRDVQKYMKQQKKEEEPLNNPFA
HCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHH
EALKKLKFD
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA