| Definition | Bacillus cereus B4264, complete genome. |
|---|---|
| Accession | NC_011725 |
| Length | 5,419,036 |
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The map label for this gene is clpB [H]
Identifier: 218235585
GI number: 218235585
Start: 1188467
End: 1191067
Strand: Direct
Name: clpB [H]
Synonym: BCB4264_A1223
Alternate gene names: 218235585
Gene position: 1188467-1191067 (Clockwise)
Preceding gene: 218232254
Following gene: 218231627
Centisome position: 21.93
GC content: 39.06
Gene sequence:
>2601_bases ATGGACTTAAATCAAATGACAACAAAAACACAAGAGGCGATTATGAGTGCCCAATCTTTAGCGGTATCTCATCATCACCA AGAGGTGGATACTGTTCATCTATTACTTGCATTATTAGAAGAGCAAGATGGGTTAGCGGTACGTATATTTCAAAAAATGA ATGTTGATATAGAAGCATTAAAGCAAGGTGCTGAAAGTTTAATTAAAAAGAAACCTTCTGTAACGGGGAGCGGTGCAGAA GTTGGAAAATTGTATGTAACGAGCGCTCTGCAACAACTGCTTGTAAGAGCAGGGAAAGAAGCAGAAAAACTGCAGGATGA TTACATTTCAGTAGAACATGTATTGCTTGCTTTTTCTGAAGAAAAAGGCGATATAAATCAATTATTTACAAGATTGCATA TTACGAAAGATAACTTATTACAGTCTTTAATGACAGTTCGGGGGAATCAAAGAGTGACGAGTCAAAATCCAGAAGCAACT TATGAAGCGTTAGAAAAATATGGTCGTGATTTAGTGGCGGAAGTGAGAGCAGGGAAAATTGATCCTGTAATCGGCCGTGA TAGTGAAATACGACGTGTAATCCGTATTCTTTCACGTAAAACGAAAAACAATCCGGTTTTAATTGGGGAGCCAGGTGTTG GTAAAACAGCAATCGTTGAAGGGCTAGCCCAGCGTATTGTGAAAAAGGATGTACCTGAAGGATTGAAAGATAGAACGATT TTTGCGTTAGATATGAGTGCACTCGTAGCTGGTGCGAAATTCCGTGGTGAGTTCGAAGAGCGTCTGCAAGCTGTATTAAA TGAAATTAAAAAGAGTGAAGGACGCATTTTATTATTCATTGATGAACTTCATACAATAGTTGGAGCTGGTAAAACAGAAG GAGCCATGGATGCAGGAAATATGTTAAAACCGATGCTTGCACGTGGTGAACTGCATTGTATTGGAGCCACAACACTAGAT GAATATCGCAAATATATTGAGAAAGATCCAGCGCTAGAAAGACGTTTCCAACAAGTATTAGCAGAAGAGCCAACTGTTGA AGATACGATTTCCATTTTACGTGGATTAAAAGAACGCTTTGAAATTTATCACGGTGTAAATATTCATGACCGTGCGATTG TAGCAGCATCTGTTTTATCAGATCGATATATTTCAGATCGCTTCTTACCAGATAAAGCAATTGATCTTGTTGACGAAGCG TGTGCAACAATTCGTACAGAAATTGATTCTATGCCAACAGAATTAGATGAAGTAACGCGCCGCATTATGCAGCTGGAAAT TGAAGAAGCGGCTCTTGGGAAAGAAACGGATCGTGGTAGCCAAGAGCGTCTAAAAACATTGCAACGTGAATTATCGGATT TAAAAGAAGTTGCCAGTGGCATGAGAGCGAAATGGGAGAAAGAAAAAGAAGACATTCACAAAGTTCGTGACTTACGTGAG CATTTAGAACGTTTGCGTCGTGAATTAGAAGAAGCAGAAGGCAATTACGATTTAAATAAAGCAGCCGAACTTCGTCACGG GAAAATTCCCGCAATTGAAAAAGAGTTAAAAGAAGCGGAAGAAATGGGCGCGCATAATAAACAAGAAAATCGTTTATTAC GTGAGGAAGTAAGTGAAGAAGAAATTGCAGATATTGTTTCACGCTGGACTGGTATTCCTGTTGCGAAACTCGTGGAAGGT GAACGCGAGAAATTATTACGCTTAGAGCAAATCTTATCAGAGCGTGTCATCGGACAAGAGGAAGCAGTAAGCTTAGTGTC AGACGCGGTTCTTCGTGCTCGCGCTGGTATTAAAGATCCGAACCGCCCGATTGGTTCTTTCATCTTCTTAGGGCCAACAG GTGTTGGTAAAACAGAACTTGCAAAAACGTTAGCCCAGTCTTTATTCGATAGTGAAGAGCAAATGATTCGCATTGACATG TCTGAGTATATGGAAAAACACGCCGTGTCTCGCTTAATTGGTGCACCTCCTGGATATGTAGGATATGAAGAAGGCGGTCA ATTAACAGAAGCGGTAAGACGTAAACCATATTCCGTTGTTTTATTAGATGAAATTGAAAAAGCACATCCAGAAGTATTCA ACATTTTATTACAAATGTTAGATGATGGACGCATTACAGATTCGCAAGGTCGTACAGTGGACTTTAAAAATACAGTTATT ATTATGACTTCAAATATTGGATCTGCTCATTTACTAGATGGATTAGAAGAAGATGGTTCGATTAAAGAGGAATCAAGAGA CCTTGTAATGGGGCAATTAAGAGGACATTTCCGCCCTGAATTTTTAAACCGTGTGGATGAAATTATTTTATTCAAACCTC TTACAACGAATGAAATTAAAGGCATTGTTGATAAAATTGTAAAAGAACTACAAGGTCGTCTAGCTGACCGTCACATTACA GTAGAATTAACAGATGCAGCAAAAGAATTTGTTGTGGAAGCCGGTTTCGACCCAATGTACGGAGCTCGTCCATTAAAACG ATACGTACAGCGTCAAGTGGAAACGAAATTAGCACGAGAATTAATTGCAGGAACAATTACTGATAATAGTCACGTAGTTG TTGATGTAGAAAATAACGAATTAGTCGTTCATGTGAAATAA
Upstream 100 bases:
>100_bases AGTTTGAAAATACATAGGCAAAATTATTGTATAAAAAAGTCAGTTGGCGTATAATCAAATCAAAGGTCAAAGAAAGTCAA ACTTTTGGAGGGCTGTAAAG
Downstream 100 bases:
>100_bases AAATAAAAAAGAGTTCGGATAAAATCCGGACTCTTTTTTTAGTGTTGTTCTACTGGCTCGTTTGGAATTGCAGCTGAAAT TGCTAGTACTAACACAGCAA
Product: ATP-dependent Clp protease, ATP-binding subunit ClpB
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 866; Mature: 866
Protein sequence:
>866_residues MDLNQMTTKTQEAIMSAQSLAVSHHHQEVDTVHLLLALLEEQDGLAVRIFQKMNVDIEALKQGAESLIKKKPSVTGSGAE VGKLYVTSALQQLLVRAGKEAEKLQDDYISVEHVLLAFSEEKGDINQLFTRLHITKDNLLQSLMTVRGNQRVTSQNPEAT YEALEKYGRDLVAEVRAGKIDPVIGRDSEIRRVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVKKDVPEGLKDRTI FALDMSALVAGAKFRGEFEERLQAVLNEIKKSEGRILLFIDELHTIVGAGKTEGAMDAGNMLKPMLARGELHCIGATTLD EYRKYIEKDPALERRFQQVLAEEPTVEDTISILRGLKERFEIYHGVNIHDRAIVAASVLSDRYISDRFLPDKAIDLVDEA CATIRTEIDSMPTELDEVTRRIMQLEIEEAALGKETDRGSQERLKTLQRELSDLKEVASGMRAKWEKEKEDIHKVRDLRE HLERLRRELEEAEGNYDLNKAAELRHGKIPAIEKELKEAEEMGAHNKQENRLLREEVSEEEIADIVSRWTGIPVAKLVEG EREKLLRLEQILSERVIGQEEAVSLVSDAVLRARAGIKDPNRPIGSFIFLGPTGVGKTELAKTLAQSLFDSEEQMIRIDM SEYMEKHAVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVVLLDEIEKAHPEVFNILLQMLDDGRITDSQGRTVDFKNTVI IMTSNIGSAHLLDGLEEDGSIKEESRDLVMGQLRGHFRPEFLNRVDEIILFKPLTTNEIKGIVDKIVKELQGRLADRHIT VELTDAAKEFVVEAGFDPMYGARPLKRYVQRQVETKLARELIAGTITDNSHVVVDVENNELVVHVK
Sequences:
>Translated_866_residues MDLNQMTTKTQEAIMSAQSLAVSHHHQEVDTVHLLLALLEEQDGLAVRIFQKMNVDIEALKQGAESLIKKKPSVTGSGAE VGKLYVTSALQQLLVRAGKEAEKLQDDYISVEHVLLAFSEEKGDINQLFTRLHITKDNLLQSLMTVRGNQRVTSQNPEAT YEALEKYGRDLVAEVRAGKIDPVIGRDSEIRRVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVKKDVPEGLKDRTI FALDMSALVAGAKFRGEFEERLQAVLNEIKKSEGRILLFIDELHTIVGAGKTEGAMDAGNMLKPMLARGELHCIGATTLD EYRKYIEKDPALERRFQQVLAEEPTVEDTISILRGLKERFEIYHGVNIHDRAIVAASVLSDRYISDRFLPDKAIDLVDEA CATIRTEIDSMPTELDEVTRRIMQLEIEEAALGKETDRGSQERLKTLQRELSDLKEVASGMRAKWEKEKEDIHKVRDLRE HLERLRRELEEAEGNYDLNKAAELRHGKIPAIEKELKEAEEMGAHNKQENRLLREEVSEEEIADIVSRWTGIPVAKLVEG EREKLLRLEQILSERVIGQEEAVSLVSDAVLRARAGIKDPNRPIGSFIFLGPTGVGKTELAKTLAQSLFDSEEQMIRIDM SEYMEKHAVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVVLLDEIEKAHPEVFNILLQMLDDGRITDSQGRTVDFKNTVI IMTSNIGSAHLLDGLEEDGSIKEESRDLVMGQLRGHFRPEFLNRVDEIILFKPLTTNEIKGIVDKIVKELQGRLADRHIT VELTDAAKEFVVEAGFDPMYGARPLKRYVQRQVETKLARELIAGTITDNSHVVVDVENNELVVHVK >Mature_866_residues MDLNQMTTKTQEAIMSAQSLAVSHHHQEVDTVHLLLALLEEQDGLAVRIFQKMNVDIEALKQGAESLIKKKPSVTGSGAE VGKLYVTSALQQLLVRAGKEAEKLQDDYISVEHVLLAFSEEKGDINQLFTRLHITKDNLLQSLMTVRGNQRVTSQNPEAT YEALEKYGRDLVAEVRAGKIDPVIGRDSEIRRVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVKKDVPEGLKDRTI FALDMSALVAGAKFRGEFEERLQAVLNEIKKSEGRILLFIDELHTIVGAGKTEGAMDAGNMLKPMLARGELHCIGATTLD EYRKYIEKDPALERRFQQVLAEEPTVEDTISILRGLKERFEIYHGVNIHDRAIVAASVLSDRYISDRFLPDKAIDLVDEA CATIRTEIDSMPTELDEVTRRIMQLEIEEAALGKETDRGSQERLKTLQRELSDLKEVASGMRAKWEKEKEDIHKVRDLRE HLERLRRELEEAEGNYDLNKAAELRHGKIPAIEKELKEAEEMGAHNKQENRLLREEVSEEEIADIVSRWTGIPVAKLVEG EREKLLRLEQILSERVIGQEEAVSLVSDAVLRARAGIKDPNRPIGSFIFLGPTGVGKTELAKTLAQSLFDSEEQMIRIDM SEYMEKHAVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVVLLDEIEKAHPEVFNILLQMLDDGRITDSQGRTVDFKNTVI IMTSNIGSAHLLDGLEEDGSIKEESRDLVMGQLRGHFRPEFLNRVDEIILFKPLTTNEIKGIVDKIVKELQGRLADRHIT VELTDAAKEFVVEAGFDPMYGARPLKRYVQRQVETKLARELIAGTITDNSHVVVDVENNELVVHVK
Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase
COG id: COG0542
COG function: function code O; ATPases with chaperone activity, ATP-binding subunit
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the clpA/clpB family [H]
Homologues:
Organism=Homo sapiens, GI13540606, Length=311, Percent_Identity=39.5498392282958, Blast_Score=216, Evalue=1e-55, Organism=Escherichia coli, GI1788943, Length=862, Percent_Identity=53.8283062645012, Blast_Score=925, Evalue=0.0, Organism=Escherichia coli, GI1787109, Length=331, Percent_Identity=43.202416918429, Blast_Score=280, Evalue=2e-76, Organism=Saccharomyces cerevisiae, GI6320464, Length=712, Percent_Identity=52.3876404494382, Blast_Score=748, Evalue=0.0, Organism=Saccharomyces cerevisiae, GI6323002, Length=866, Percent_Identity=42.0323325635104, Blast_Score=677, Evalue=0.0,
Paralogues:
None
Copy number: 560 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003593 - InterPro: IPR013093 - InterPro: IPR003959 - InterPro: IPR018368 - InterPro: IPR017730 - InterPro: IPR001270 - InterPro: IPR019489 - InterPro: IPR004176 - InterPro: IPR023150 [H]
Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]
EC number: NA
Molecular weight: Translated: 97384; Mature: 97384
Theoretical pI: Translated: 5.16; Mature: 5.16
Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 2.5 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MDLNQMTTKTQEAIMSAQSLAVSHHHQEVDTVHLLLALLEEQDGLAVRIFQKMNVDIEAL CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCHHHH KQGAESLIKKKPSVTGSGAEVGKLYVTSALQQLLVRAGKEAEKLQDDYISVEHVLLAFSE HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC EKGDINQLFTRLHITKDNLLQSLMTVRGNQRVTSQNPEATYEALEKYGRDLVAEVRAGKI CCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC DPVIGRDSEIRRVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVKKDVPEGLKDRTI CCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEE FALDMSALVAGAKFRGEFEERLQAVLNEIKKSEGRILLFIDELHTIVGAGKTEGAMDAGN EEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCHH MLKPMLARGELHCIGATTLDEYRKYIEKDPALERRFQQVLAEEPTVEDTISILRGLKERF HHHHHHHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH EIYHGVNIHDRAIVAASVLSDRYISDRFLPDKAIDLVDEACATIRTEIDSMPTELDEVTR HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH RIMQLEIEEAALGKETDRGSQERLKTLQRELSDLKEVASGMRAKWEKEKEDIHKVRDLRE HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HLERLRRELEEAEGNYDLNKAAELRHGKIPAIEKELKEAEEMGAHNKQENRLLREEVSEE HHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHH EIADIVSRWTGIPVAKLVEGEREKLLRLEQILSERVIGQEEAVSLVSDAVLRARAGIKDP HHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC NRPIGSFIFLGPTGVGKTELAKTLAQSLFDSEEQMIRIDMSEYMEKHAVSRLIGAPPGYV CCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEECHHHHHHHHHHHHHHCCCCCCC GYEEGGQLTEAVRRKPYSVVLLDEIEKAHPEVFNILLQMLDDGRITDSQGRTVDFKNTVI CCCCCCHHHHHHHCCCCEEEEHHHHHHHCHHHHHHHHHHHCCCCEECCCCCEEEECCEEE IMTSNIGSAHLLDGLEEDGSIKEESRDLVMGQLRGHFRPEFLNRVDEIILFKPLTTNEIK EEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHEEEECCCCHHHHH GIVDKIVKELQGRLADRHITVELTDAAKEFVVEAGFDPMYGARPLKRYVQRQVETKLARE HHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH LIAGTITDNSHVVVDVENNELVVHVK HHHHEECCCCEEEEEECCCEEEEEEC >Mature Secondary Structure MDLNQMTTKTQEAIMSAQSLAVSHHHQEVDTVHLLLALLEEQDGLAVRIFQKMNVDIEAL CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCHHHH KQGAESLIKKKPSVTGSGAEVGKLYVTSALQQLLVRAGKEAEKLQDDYISVEHVLLAFSE HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC EKGDINQLFTRLHITKDNLLQSLMTVRGNQRVTSQNPEATYEALEKYGRDLVAEVRAGKI CCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC DPVIGRDSEIRRVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVKKDVPEGLKDRTI CCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEE FALDMSALVAGAKFRGEFEERLQAVLNEIKKSEGRILLFIDELHTIVGAGKTEGAMDAGN EEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCHH MLKPMLARGELHCIGATTLDEYRKYIEKDPALERRFQQVLAEEPTVEDTISILRGLKERF HHHHHHHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH EIYHGVNIHDRAIVAASVLSDRYISDRFLPDKAIDLVDEACATIRTEIDSMPTELDEVTR HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH RIMQLEIEEAALGKETDRGSQERLKTLQRELSDLKEVASGMRAKWEKEKEDIHKVRDLRE HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HLERLRRELEEAEGNYDLNKAAELRHGKIPAIEKELKEAEEMGAHNKQENRLLREEVSEE HHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHH EIADIVSRWTGIPVAKLVEGEREKLLRLEQILSERVIGQEEAVSLVSDAVLRARAGIKDP HHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC NRPIGSFIFLGPTGVGKTELAKTLAQSLFDSEEQMIRIDMSEYMEKHAVSRLIGAPPGYV CCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEECHHHHHHHHHHHHHHCCCCCCC GYEEGGQLTEAVRRKPYSVVLLDEIEKAHPEVFNILLQMLDDGRITDSQGRTVDFKNTVI CCCCCCHHHHHHHCCCCEEEEHHHHHHHCHHHHHHHHHHHCCCCEECCCCCEEEECCEEE IMTSNIGSAHLLDGLEEDGSIKEESRDLVMGQLRGHFRPEFLNRVDEIILFKPLTTNEIK EEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHEEEECCCCHHHHH GIVDKIVKELQGRLADRHITVELTDAAKEFVVEAGFDPMYGARPLKRYVQRQVETKLARE HHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH LIAGTITDNSHVVVDVENNELVVHVK HHHHEECCCCEEEEEECCCEEEEEEC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12721629 [H]