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The map label for this gene is vanS [H]

Identifier: 218235425

GI number: 218235425

Start: 1777485

End: 1778360

Strand: Direct

Name: vanS [H]

Synonym: BCB4264_A1870

Alternate gene names: 218235425

Gene position: 1777485-1778360 (Clockwise)

Preceding gene: 218236009

Following gene: 218233171

Centisome position: 32.8

GC content: 30.94

Gene sequence:

>876_bases
ATGACAAAAACACTGATGAAAAGGTTGTCTCAAATTAACAAGAACGTGAAGGAAATAAGTGACGGTAATTTTGAAATACA
TATACCTATTTTGAAAAATGACGAGATTGGTGAACTAGCGGCAAATGTTAATAGAATGGCGAAAAGTTTAAAAGAGTCTA
TCGAGAATGAAAAAAAATCACAGGAAATGAAAAATGAAATGATTAGTAATATTTCTCATGACTTACGAACGCCAGTAACA
TCTTTAATCGGTTATGCGGACCTGTTAGGAAATAAACTTCATTCGAATGGAGAAGAATGTGAACAGTATGTAAGTATATT
AAAGCGAAAAAGTTATGAATTAAAAAATCAAGTAGATGATTTATTAGAATACTGTCAAATAAATTATAGAGAGATTGAAT
TACATAAAGATGAAGTAGATATGAAAGCATTAATTGAACAAATCATGATTGACTTTGTACCACAATTAGATGATGCCAAT
ATGAGCTTTTATATTAAAAGTGATGAAGTGCTACATGTAGAAGTTGATGTGACACTTATAGTGAGACTATTTGAAAATGT
AATAGGTAATAGTATTATGTACGGGAGAGATGGAAAGGGAATTGTAATTGAAATTTTCAATAAAACAATGAATGTTGAAG
TAGAAATTAAAAATTTTGGACAATGCATTCCGAAAGAGGACCTTCCTTACATTTTTGAGAAGTTTTATCGTAGTGAAAAA
TCACGTAGTTCTCATACGGGCGGTAAAGGAATAGGACTTGCAATTTCAAAAAGTATAGCCGAGCTCCATCAAGGGGATAT
CACTGTACGTAGTAATGATAAAGAAACAGTTTTTACTGTGAAATTACCGCAGTATAAAAAAGTAAGAAAGTCGTAA

Upstream 100 bases:

>100_bases
GGTTGGAAATATATATAGTCAGGTTAATGTTTTAGAGGAAAAATATTCATTTTTATATTTTATAGTTTTTTTGATTTTTA
CTACAACATACTTTGCATTA

Downstream 100 bases:

>100_bases
GTATTTCTTTTATATGTCGTAATTTTTACTTTGTATCATTGAGAAAAAGGATATGAGAGTAGGGATGCGACATGTGGAAG
AAATTTTTATTAGTAATTAT

Product: sensor protein VanS

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTKTLMKRLSQINKNVKEISDGNFEIHIPILKNDEIGELAANVNRMAKSLKESIENEKKSQEMKNEMISNISHDLRTPVT
SLIGYADLLGNKLHSNGEECEQYVSILKRKSYELKNQVDDLLEYCQINYREIELHKDEVDMKALIEQIMIDFVPQLDDAN
MSFYIKSDEVLHVEVDVTLIVRLFENVIGNSIMYGRDGKGIVIEIFNKTMNVEVEIKNFGQCIPKEDLPYIFEKFYRSEK
SRSSHTGGKGIGLAISKSIAELHQGDITVRSNDKETVFTVKLPQYKKVRKS

Sequences:

>Translated_291_residues
MTKTLMKRLSQINKNVKEISDGNFEIHIPILKNDEIGELAANVNRMAKSLKESIENEKKSQEMKNEMISNISHDLRTPVT
SLIGYADLLGNKLHSNGEECEQYVSILKRKSYELKNQVDDLLEYCQINYREIELHKDEVDMKALIEQIMIDFVPQLDDAN
MSFYIKSDEVLHVEVDVTLIVRLFENVIGNSIMYGRDGKGIVIEIFNKTMNVEVEIKNFGQCIPKEDLPYIFEKFYRSEK
SRSSHTGGKGIGLAISKSIAELHQGDITVRSNDKETVFTVKLPQYKKVRKS
>Mature_290_residues
TKTLMKRLSQINKNVKEISDGNFEIHIPILKNDEIGELAANVNRMAKSLKESIENEKKSQEMKNEMISNISHDLRTPVTS
LIGYADLLGNKLHSNGEECEQYVSILKRKSYELKNQVDDLLEYCQINYREIELHKDEVDMKALIEQIMIDFVPQLDDANM
SFYIKSDEVLHVEVDVTLIVRLFENVIGNSIMYGRDGKGIVIEIFNKTMNVEVEIKNFGQCIPKEDLPYIFEKFYRSEKS
RSSHTGGKGIGLAISKSIAELHQGDITVRSNDKETVFTVKLPQYKKVRKS

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=289, Percent_Identity=25.6055363321799, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786783, Length=273, Percent_Identity=26.007326007326, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI145693157, Length=244, Percent_Identity=23.7704918032787, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789149, Length=255, Percent_Identity=24.7058823529412, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786600, Length=222, Percent_Identity=25.6756756756757, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1786912, Length=236, Percent_Identity=26.6949152542373, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994928, Length=234, Percent_Identity=27.7777777777778, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082128, Length=239, Percent_Identity=23.0125523012552, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788279, Length=255, Percent_Identity=25.0980392156863, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787894, Length=282, Percent_Identity=24.113475177305, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1790861, Length=273, Percent_Identity=21.2454212454212, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790436, Length=242, Percent_Identity=23.5537190082645, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788713, Length=248, Percent_Identity=21.7741935483871, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790346, Length=276, Percent_Identity=22.463768115942, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788549, Length=231, Percent_Identity=22.9437229437229, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 33411; Mature: 33280

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTLMKRLSQINKNVKEISDGNFEIHIPILKNDEIGELAANVNRMAKSLKESIENEKKS
CCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QEMKNEMISNISHDLRTPVTSLIGYADLLGNKLHSNGEECEQYVSILKRKSYELKNQVDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLEYCQINYREIELHKDEVDMKALIEQIMIDFVPQLDDANMSFYIKSDEVLHVEVDVTLI
HHHHHCCCCCEEEEEHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEHHHHH
VRLFENVIGNSIMYGRDGKGIVIEIFNKTMNVEVEIKNFGQCIPKEDLPYIFEKFYRSEK
HHHHHHHHCCCEEECCCCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
SRSSHTGGKGIGLAISKSIAELHQGDITVRSNDKETVFTVKLPQYKKVRKS
CCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCHHHHHCC
>Mature Secondary Structure 
TKTLMKRLSQINKNVKEISDGNFEIHIPILKNDEIGELAANVNRMAKSLKESIENEKKS
CHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QEMKNEMISNISHDLRTPVTSLIGYADLLGNKLHSNGEECEQYVSILKRKSYELKNQVDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLEYCQINYREIELHKDEVDMKALIEQIMIDFVPQLDDANMSFYIKSDEVLHVEVDVTLI
HHHHHCCCCCEEEEEHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEHHHHH
VRLFENVIGNSIMYGRDGKGIVIEIFNKTMNVEVEIKNFGQCIPKEDLPYIFEKFYRSEK
HHHHHHHHCCCEEECCCCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
SRSSHTGGKGIGLAISKSIAELHQGDITVRSNDKETVFTVKLPQYKKVRKS
CCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]