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The map label for this gene is ykuM [H]

Identifier: 218235358

GI number: 218235358

Start: 4000766

End: 4001650

Strand: Reverse

Name: ykuM [H]

Synonym: BCB4264_A4085

Alternate gene names: 218235358

Gene position: 4001650-4000766 (Counterclockwise)

Preceding gene: 218231895

Following gene: 218236139

Centisome position: 73.84

GC content: 35.14

Gene sequence:

>885_bases
ATGGAAGCTAAGGAAAGAGGGAGAAAGTTGCAAATTGATGATTTTCAAATGATGGTTGTGTTAGCTCAGGAATCAAATAT
GAGAAAAGCGGCGGAACGTTTATTTGTTTCCCAACCTGCGCTGAGTCAACGATTACAATCTATGGAGAAACAATGGGGAA
TGAAGTTTTTTATTCGCTCACAAAAAGGATTGACAATTACACCTGAAGGGGAAAAAGTTGCAAATTATGCCAAAGAAATG
TTGCAAAGGGAAGAAGATATAAAAAGTGAGCTGGCTGTTTTCAGAACGGAAACTCATGGAACGTTAAAAATAGCTGTTGC
ATCAGTTATTGGTCAATATTGGCTCCCTCCTGTTTTGAAAAAGTTTGTGCATAAATATCCTTCTGTAAAGATATCGCTGT
TTACTGGATGGAGTAGTGAAGTGCAAAAGCAGTTTTATGAAGGAGACGTGCATGTTGCTATACTGAGGGGAACACAAGAA
TATAAAGGTCAGAAGCAACAATTATTTGAAGATGAACTGTATTTAGTTGATAAAGAAATAAAAGATATTTCGATGTTAAA
AGAAACAAATAGGCCGTTCATTCAATTTAAAAGTGACTCGACTTATTATGGACAAATACAAAATTGGTGGTATGGTTTAT
TTTCTAATCCGCCTAAGCGAACGATTGTAGTTGACCAAATTGAAACATGTAAACAACTTGTTTTAAATGGTATAGGTTAT
GCCCTTTTACCTTCTACTGTTTTAAAGGAAGTACAGGAAAATATGTATAAAACACCTGTTCAACTGACGAGAGAAACATG
GTTGTTAACAAGTGACTCGGCAAGGCAGTTGAAGCAAGTACAAGCATTTTTAGAAATTATAGAAGAAATTCAAAGGGAAA
AATAA

Upstream 100 bases:

>100_bases
AAGACATAAAACATAAGGAAAGTTTGCGCTGGACAATTGTATTTTCTGAATGAAAGCATTCGGATAAATTAAGGATGAAA
AGCTTATTGAAATAAGGTGT

Downstream 100 bases:

>100_bases
GATTATTATCTTGCTACTCGCTCTTTCGTTTGGTAGAGTAACATTATAAATGGATTTAGGAGGCAACAAGAATGAAAATG
ATGGACGCTAACGAAATTAT

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MEAKERGRKLQIDDFQMMVVLAQESNMRKAAERLFVSQPALSQRLQSMEKQWGMKFFIRSQKGLTITPEGEKVANYAKEM
LQREEDIKSELAVFRTETHGTLKIAVASVIGQYWLPPVLKKFVHKYPSVKISLFTGWSSEVQKQFYEGDVHVAILRGTQE
YKGQKQQLFEDELYLVDKEIKDISMLKETNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGY
ALLPSTVLKEVQENMYKTPVQLTRETWLLTSDSARQLKQVQAFLEIIEEIQREK

Sequences:

>Translated_294_residues
MEAKERGRKLQIDDFQMMVVLAQESNMRKAAERLFVSQPALSQRLQSMEKQWGMKFFIRSQKGLTITPEGEKVANYAKEM
LQREEDIKSELAVFRTETHGTLKIAVASVIGQYWLPPVLKKFVHKYPSVKISLFTGWSSEVQKQFYEGDVHVAILRGTQE
YKGQKQQLFEDELYLVDKEIKDISMLKETNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGY
ALLPSTVLKEVQENMYKTPVQLTRETWLLTSDSARQLKQVQAFLEIIEEIQREK
>Mature_294_residues
MEAKERGRKLQIDDFQMMVVLAQESNMRKAAERLFVSQPALSQRLQSMEKQWGMKFFIRSQKGLTITPEGEKVANYAKEM
LQREEDIKSELAVFRTETHGTLKIAVASVIGQYWLPPVLKKFVHKYPSVKISLFTGWSSEVQKQFYEGDVHVAILRGTQE
YKGQKQQLFEDELYLVDKEIKDISMLKETNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGY
ALLPSTVLKEVQENMYKTPVQLTRETWLLTSDSARQLKQVQAFLEIIEEIQREK

Specific function: This Protein Is A Positive Regulator Of Gene Expression For The Cysteine Regulon, A System Of 10 Or More Loci Involved In The Biosynthesis Of L-Cysteine From Inorganic Sulfate. The Inducer For Cysb Is N-Acetylserine. Cysb Inhibits Its Own Transcription.

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=157, Percent_Identity=29.9363057324841, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787806, Length=292, Percent_Identity=20.2054794520548, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786401, Length=250, Percent_Identity=27.2, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI157672245, Length=115, Percent_Identity=33.0434782608696, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788296, Length=133, Percent_Identity=27.0676691729323, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788706, Length=130, Percent_Identity=32.3076923076923, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790399, Length=244, Percent_Identity=22.5409836065574, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790208, Length=253, Percent_Identity=23.7154150197628, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=24.6575342465753, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788481, Length=230, Percent_Identity=26.0869565217391, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787589, Length=140, Percent_Identity=26.4285714285714, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693105, Length=126, Percent_Identity=25.3968253968254, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787128, Length=148, Percent_Identity=23.6486486486486, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788887, Length=247, Percent_Identity=24.2914979757085, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789506, Length=251, Percent_Identity=21.1155378486056, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789173, Length=152, Percent_Identity=26.3157894736842, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34319; Mature: 34319

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAKERGRKLQIDDFQMMVVLAQESNMRKAAERLFVSQPALSQRLQSMEKQWGMKFFIRS
CCCHHCCCEECCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEC
QKGLTITPEGEKVANYAKEMLQREEDIKSELAVFRTETHGTLKIAVASVIGQYWLPPVLK
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHH
KFVHKYPSVKISLFTGWSSEVQKQFYEGDVHVAILRGTQEYKGQKQQLFEDELYLVDKEI
HHHHHCCCEEEEEEECCCHHHHHHHHCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHHH
KDISMLKETNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGY
HHHHHHHHCCCCEEEECCCCCEEECHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCH
ALLPSTVLKEVQENMYKTPVQLTRETWLLTSDSARQLKQVQAFLEIIEEIQREK
HHHHHHHHHHHHHHHHCCCHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEAKERGRKLQIDDFQMMVVLAQESNMRKAAERLFVSQPALSQRLQSMEKQWGMKFFIRS
CCCHHCCCEECCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEC
QKGLTITPEGEKVANYAKEMLQREEDIKSELAVFRTETHGTLKIAVASVIGQYWLPPVLK
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHH
KFVHKYPSVKISLFTGWSSEVQKQFYEGDVHVAILRGTQEYKGQKQQLFEDELYLVDKEI
HHHHHCCCEEEEEEECCCHHHHHHHHCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHHH
KDISMLKETNRPFIQFKSDSTYYGQIQNWWYGLFSNPPKRTIVVDQIETCKQLVLNGIGY
HHHHHHHHCCCCEEEECCCCCEEECHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCH
ALLPSTVLKEVQENMYKTPVQLTRETWLLTSDSARQLKQVQAFLEIIEEIQREK
HHHHHHHHHHHHHHHHCCCHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]