BacMap

Definition	Bacillus cereus B4264, complete genome.
Accession	NC_011725
Length	5,419,036

Click here to switch to the map view.

The map label for this gene is gmk [H]

Identifier: 218235330

GI number: 218235330

Start: 3892684

End: 3893301

Strand: Reverse

Name: gmk [H]

Synonym: BCB4264_A3970

Alternate gene names: 218235330

Gene position: 3893301-3892684 (Counterclockwise)

Preceding gene: 218233398

Following gene: 218236091

Centisome position: 71.84

GC content: 38.83

Gene sequence:

>618_bases
ATGAGAAGTAGAAGAGGATTGCTCATCGTTCTTTCAGGGCCTTCTGGGGTTGGAAAAGGGACGGTTCGTAAAGAGCTGTT
TAGCCATGAGGATACACGTTTTCAGTACTCTATTTCAGTAACGACACGTAAGCCGCGTGAAGGTGAAGTAGATGGTGTGG
ATTATTTCTTTAAAGAAAGAGAAGAATTCGAGGAAATGATTCGTAATGAAAAATTACTTGAGTGGGCTGAGTTCGTAGGT
AATTATTACGGAACACCGATTGACTATGTTGAAAAAACATTACAAGAAGGAAAAGATGTATTCTTAGAAATTGAAGTGCA
AGGAGCAATTCAAGTTAAGAAAGCTTTCCCAGAAGGTGTATTTATTTTCTTAGCACCTCCAAGTTTATCTGAACTAAAGA
GCCGTATTGTCGGACGTGGTACAGAGACTGAAGATGTTATTGAAAATCGTTTAACTGTAGCGAAAGAAGAAATCGAGATG
ATGGACGCTTACGACTATGTAGTAGAAAACGATCAAGTTGAATTAGCTTGTGATAGAATTAAAGCAATTGTGGTTGGCGA
ACATTGCCGCCGCGAAAGGGTAGCAAAATATTATAAAGAAATGACGGAGGGTCTATAA

Upstream 100 bases:

>100_bases
GATGGGCATGTTGTATTAAGTCCAATTCAACCAGAGACGATTGCACATCGTTTGAATAATAAAGAAGATTTAAGTGAGGA
AGGGTAGGTTTTACATATTT

Downstream 100 bases:

>100_bases
TTATGTTAAATCCATCAATTGACTCATTACTACAAAAAATCGATTCTAAGTACACTTTAGTAACAGTGGCTGCAAAACGC
GCACGTGAAATGCAACTTGC

Product: guanylate kinase

Products: NA

Alternate protein names: GMP kinase [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MRSRRGLLIVLSGPSGVGKGTVRKELFSHEDTRFQYSISVTTRKPREGEVDGVDYFFKEREEFEEMIRNEKLLEWAEFVG
NYYGTPIDYVEKTLQEGKDVFLEIEVQGAIQVKKAFPEGVFIFLAPPSLSELKSRIVGRGTETEDVIENRLTVAKEEIEM
MDAYDYVVENDQVELACDRIKAIVVGEHCRRERVAKYYKEMTEGL

Sequences:

>Translated_205_residues
MRSRRGLLIVLSGPSGVGKGTVRKELFSHEDTRFQYSISVTTRKPREGEVDGVDYFFKEREEFEEMIRNEKLLEWAEFVG
NYYGTPIDYVEKTLQEGKDVFLEIEVQGAIQVKKAFPEGVFIFLAPPSLSELKSRIVGRGTETEDVIENRLTVAKEEIEM
MDAYDYVVENDQVELACDRIKAIVVGEHCRRERVAKYYKEMTEGL
>Mature_205_residues
MRSRRGLLIVLSGPSGVGKGTVRKELFSHEDTRFQYSISVTTRKPREGEVDGVDYFFKEREEFEEMIRNEKLLEWAEFVG
NYYGTPIDYVEKTLQEGKDVFLEIEVQGAIQVKKAFPEGVFIFLAPPSLSELKSRIVGRGTETEDVIENRLTVAKEEIEM
MDAYDYVVENDQVELACDRIKAIVVGEHCRRERVAKYYKEMTEGL

Specific function: Essential for recycling GMP and indirectly, cGMP [H]

COG id: COG0194

COG function: function code F; Guanylate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 guanylate kinase-like domain [H]

Homologues:

Organism=Homo sapiens, GI226874833, Length=176, Percent_Identity=36.9318181818182, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI4504221, Length=176, Percent_Identity=36.9318181818182, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI226874831, Length=176, Percent_Identity=36.9318181818182, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI14780902, Length=183, Percent_Identity=30.0546448087432, Blast_Score=93, Evalue=1e-19,
Organism=Homo sapiens, GI38570142, Length=145, Percent_Identity=34.4827586206897, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI21361598, Length=184, Percent_Identity=29.8913043478261, Blast_Score=84, Evalue=7e-17,
Organism=Homo sapiens, GI4505237, Length=136, Percent_Identity=33.0882352941176, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI262118255, Length=136, Percent_Identity=33.0882352941176, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI262118259, Length=136, Percent_Identity=33.0882352941176, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI262118263, Length=136, Percent_Identity=33.0882352941176, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI119943106, Length=177, Percent_Identity=28.8135593220339, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI186700627, Length=122, Percent_Identity=28.6885245901639, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI186700629, Length=122, Percent_Identity=28.6885245901639, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI186972120, Length=122, Percent_Identity=28.6885245901639, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI111154074, Length=112, Percent_Identity=30.3571428571429, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI119943108, Length=177, Percent_Identity=28.8135593220339, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI261862237, Length=177, Percent_Identity=28.8135593220339, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI52630447, Length=126, Percent_Identity=29.3650793650794, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI192447426, Length=181, Percent_Identity=28.1767955801105, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI4557529, Length=181, Percent_Identity=28.1767955801105, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI21389483, Length=181, Percent_Identity=24.3093922651934, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1790080, Length=204, Percent_Identity=40.6862745098039, Blast_Score=155, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17508835, Length=172, Percent_Identity=40.1162790697674, Blast_Score=125, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI193203132, Length=172, Percent_Identity=40.1162790697674, Blast_Score=125, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71988048, Length=130, Percent_Identity=32.3076923076923, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556244, Length=123, Percent_Identity=34.9593495934959, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71988041, Length=130, Percent_Identity=32.3076923076923, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71984092, Length=118, Percent_Identity=29.6610169491525, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17557440, Length=121, Percent_Identity=29.7520661157025, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320662, Length=183, Percent_Identity=45.3551912568306, Blast_Score=162, Evalue=3e-41,
Organism=Drosophila melanogaster, GI21356141, Length=182, Percent_Identity=40.1098901098901, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI161077626, Length=176, Percent_Identity=28.9772727272727, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24640542, Length=176, Percent_Identity=28.9772727272727, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45554489, Length=176, Percent_Identity=28.9772727272727, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45554481, Length=176, Percent_Identity=28.9772727272727, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI85724806, Length=176, Percent_Identity=28.9772727272727, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI116007136, Length=176, Percent_Identity=28.9772727272727, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI85816281, Length=128, Percent_Identity=33.59375, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI85816106, Length=128, Percent_Identity=33.59375, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45551002, Length=128, Percent_Identity=33.59375, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221330166, Length=154, Percent_Identity=32.4675324675325, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221330162, Length=154, Percent_Identity=32.4675324675325, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221330164, Length=154, Percent_Identity=32.4675324675325, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45554804, Length=179, Percent_Identity=27.3743016759777, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI281360700, Length=179, Percent_Identity=27.3743016759777, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24641282, Length=179, Percent_Identity=27.3743016759777, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI45554818, Length=179, Percent_Identity=27.3743016759777, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24641280, Length=179, Percent_Identity=27.3743016759777, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI161077752, Length=179, Percent_Identity=27.3743016759777, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45554813, Length=179, Percent_Identity=27.3743016759777, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45554791, Length=179, Percent_Identity=27.3743016759777, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008144
- InterPro:   IPR008145
- InterPro:   IPR020590
- InterPro:   IPR017665 [H]

Pfam domain/function: PF00625 Guanylate_kin [H]

EC number: =2.7.4.8 [H]

Molecular weight: Translated: 23650; Mature: 23650

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00856 GUANYLATE_KINASE_1 ; PS50052 GUANYLATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSRRGLLIVLSGPSGVGKGTVRKELFSHEDTRFQYSISVTTRKPREGEVDGVDYFFKER
CCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHH
EEFEEMIRNEKLLEWAEFVGNYYGTPIDYVEKTLQEGKDVFLEIEVQGAIQVKKAFPEGV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCE
FIFLAPPSLSELKSRIVGRGTETEDVIENRLTVAKEEIEMMDAYDYVVENDQVELACDRI
EEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KAIVVGEHCRRERVAKYYKEMTEGL
HHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSRRGLLIVLSGPSGVGKGTVRKELFSHEDTRFQYSISVTTRKPREGEVDGVDYFFKER
CCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHH
EEFEEMIRNEKLLEWAEFVGNYYGTPIDYVEKTLQEGKDVFLEIEVQGAIQVKKAFPEGV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCE
FIFLAPPSLSELKSRIVGRGTETEDVIENRLTVAKEEIEMMDAYDYVVENDQVELACDRI
EEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KAIVVGEHCRRERVAKYYKEMTEGL
HHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]