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The map label for this gene is isp [H]

Identifier: 218235228

GI number: 218235228

Start: 3500961

End: 3501857

Strand: Reverse

Name: isp [H]

Synonym: BCB4264_A3593

Alternate gene names: 218235228

Gene position: 3501857-3500961 (Counterclockwise)

Preceding gene: 218235907

Following gene: 218231594

Centisome position: 64.62

GC content: 39.69

Gene sequence:

>897_bases
ATGAAGTTTAAAATCCAATCAGATGTAATTGATATTCAATCAGTGGCACCTATACAAGTAATTGATTGGGGTGTTAATTT
GATTCAAGCTCCTCAAATGTGGTCAATTACAAAAGGTGAGGGGATTAGGGTTGCTATACTAGATACGGGTATCGATGCTA
CCCATCCGGATCTTGCTGCCAATTACAAAAAAGGAATGAATTTTACTACAAATAATTTTACTGACATAATGGATAGGAAG
GGGCACGGCACGCACTGTGCAGGTATAATTGCTGGGTGTGACAACTCAATTGGTATCGTTGGGGTTGCTCCTAAAGCTGA
GCTTTACATTGCTAAGGTATTAGCAGACGATGGTTCTGGTTTAGTTGAGGCAATTGTAAAGGGAATTGATTGGGCTATTT
CTGAACAAGTAGATATCATTTCTATGAGTCTAGGTTCTTCGAATGATCCTGGTCCTGTCCTACATGATGCAATTAAACGT
GCCCATGAGGCAGGTATTATTATTGTTGCTGCTACTGGTAATGAAAATACTCATGTTGGGTGGCCAGCATCATATGATGA
GGTAATTGCAGTGGGAGCGATTAATAGAAATCTTGATAGAGCCACCTTCTCTAACTTCGGCAGTGAAACAGATGTAGCTG
CTCCAGGTGTAGATATTTATAGTACATATCCTGTCAATCGCTATGCAAAACTATCTGGTACGTCTATGGCTACACCAATG
GTAGCGGGGGTAATCGCCCTTATTCTAGCTTACTATCGTGATATTGGCAAAAAGTTAACTCCTGATCAAATCATGCAACT
AATTCGTGAGCATTCGGTTGACCTTGGAGAAAAAGGTGTCGATGATATGTTTGGTAATGGACTAGTAAATGTTTGTGAAT
TAATGAAAATTAGTTGA

Upstream 100 bases:

>100_bases
ATCTTGTAATGAAGTAGTTAGGAGATAAAATACTTTCCTATTGAACCTTCTAAAAAAAATGGTTATCTTTAGTAACTATT
ATAATTATAGGGGGATTTCA

Downstream 100 bases:

>100_bases
AAAGAGGGGGCTTCGGCTCCTTTTTTTGTTTTTAAAATTTATTTCTAGATTTTTTAAGAACCGTTTTATGTTATCTCAAA
ATTATCATATATCATAAGAG

Product: subtilisin Carlsberg

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKFKIQSDVIDIQSVAPIQVIDWGVNLIQAPQMWSITKGEGIRVAILDTGIDATHPDLAANYKKGMNFTTNNFTDIMDRK
GHGTHCAGIIAGCDNSIGIVGVAPKAELYIAKVLADDGSGLVEAIVKGIDWAISEQVDIISMSLGSSNDPGPVLHDAIKR
AHEAGIIIVAATGNENTHVGWPASYDEVIAVGAINRNLDRATFSNFGSETDVAAPGVDIYSTYPVNRYAKLSGTSMATPM
VAGVIALILAYYRDIGKKLTPDQIMQLIREHSVDLGEKGVDDMFGNGLVNVCELMKIS

Sequences:

>Translated_298_residues
MKFKIQSDVIDIQSVAPIQVIDWGVNLIQAPQMWSITKGEGIRVAILDTGIDATHPDLAANYKKGMNFTTNNFTDIMDRK
GHGTHCAGIIAGCDNSIGIVGVAPKAELYIAKVLADDGSGLVEAIVKGIDWAISEQVDIISMSLGSSNDPGPVLHDAIKR
AHEAGIIIVAATGNENTHVGWPASYDEVIAVGAINRNLDRATFSNFGSETDVAAPGVDIYSTYPVNRYAKLSGTSMATPM
VAGVIALILAYYRDIGKKLTPDQIMQLIREHSVDLGEKGVDDMFGNGLVNVCELMKIS
>Mature_298_residues
MKFKIQSDVIDIQSVAPIQVIDWGVNLIQAPQMWSITKGEGIRVAILDTGIDATHPDLAANYKKGMNFTTNNFTDIMDRK
GHGTHCAGIIAGCDNSIGIVGVAPKAELYIAKVLADDGSGLVEAIVKGIDWAISEQVDIISMSLGSSNDPGPVLHDAIKR
AHEAGIIIVAATGNENTHVGWPASYDEVIAVGAINRNLDRATFSNFGSETDVAAPGVDIYSTYPVNRYAKLSGTSMATPM
VAGVIALILAYYRDIGKKLTPDQIMQLIREHSVDLGEKGVDDMFGNGLVNVCELMKIS

Specific function: Involved in the generation of beta- and alpha-amylases from the large amylase precursor [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=239, Percent_Identity=29.7071129707113, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI20336184, Length=242, Percent_Identity=30.9917355371901, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI20336182, Length=242, Percent_Identity=30.9917355371901, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4505577, Length=242, Percent_Identity=30.9917355371901, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI20336180, Length=242, Percent_Identity=30.9917355371901, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI31317307, Length=243, Percent_Identity=28.3950617283951, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI20336190, Length=272, Percent_Identity=28.6764705882353, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI27894285, Length=272, Percent_Identity=28.6764705882353, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI20336188, Length=272, Percent_Identity=28.6764705882353, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI20336186, Length=272, Percent_Identity=28.6764705882353, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI4505579, Length=273, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25141268, Length=298, Percent_Identity=25.503355704698, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71983555, Length=298, Percent_Identity=25.503355704698, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71988321, Length=305, Percent_Identity=27.8688524590164, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71988315, Length=244, Percent_Identity=29.9180327868852, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71988297, Length=305, Percent_Identity=27.8688524590164, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71988308, Length=244, Percent_Identity=29.9180327868852, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71988301, Length=305, Percent_Identity=27.8688524590164, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71988312, Length=297, Percent_Identity=28.2828282828283, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324576, Length=276, Percent_Identity=32.9710144927536, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320775, Length=303, Percent_Identity=33.003300330033, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6319893, Length=246, Percent_Identity=35.3658536585366, Blast_Score=122, Evalue=6e-29,
Organism=Drosophila melanogaster, GI45550681, Length=288, Percent_Identity=26.3888888888889, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24650058, Length=327, Percent_Identity=24.7706422018349, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24650060, Length=327, Percent_Identity=24.7706422018349, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45553513, Length=327, Percent_Identity=24.7706422018349, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45553515, Length=327, Percent_Identity=24.7706422018349, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24650056, Length=327, Percent_Identity=24.7706422018349, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI17864552, Length=327, Percent_Identity=24.7706422018349, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 31813; Mature: 31813

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKIQSDVIDIQSVAPIQVIDWGVNLIQAPQMWSITKGEGIRVAILDTGIDATHPDLAA
CCEEECCCCEECCCCCCEEEEECCCHHCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHH
NYKKGMNFTTNNFTDIMDRKGHGTHCAGIIAGCDNSIGIVGVAPKAELYIAKVLADDGSG
HHHHCCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEEECCCCCH
LVEAIVKGIDWAISEQVDIISMSLGSSNDPGPVLHDAIKRAHEAGIIIVAATGNENTHVG
HHHHHHHHCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCC
WPASYDEVIAVGAINRNLDRATFSNFGSETDVAAPGVDIYSTYPVNRYAKLSGTSMATPM
CCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEECCCCHHHHH
VAGVIALILAYYRDIGKKLTPDQIMQLIREHSVDLGEKGVDDMFGNGLVNVCELMKIS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MKFKIQSDVIDIQSVAPIQVIDWGVNLIQAPQMWSITKGEGIRVAILDTGIDATHPDLAA
CCEEECCCCEECCCCCCEEEEECCCHHCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHH
NYKKGMNFTTNNFTDIMDRKGHGTHCAGIIAGCDNSIGIVGVAPKAELYIAKVLADDGSG
HHHHCCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEEECCCCCH
LVEAIVKGIDWAISEQVDIISMSLGSSNDPGPVLHDAIKRAHEAGIIIVAATGNENTHVG
HHHHHHHHCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCC
WPASYDEVIAVGAINRNLDRATFSNFGSETDVAAPGVDIYSTYPVNRYAKLSGTSMATPM
CCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEECCCCHHHHH
VAGVIALILAYYRDIGKKLTPDQIMQLIREHSVDLGEKGVDDMFGNGLVNVCELMKIS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1834632 [H]