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The map label for this gene is mraW [H]

Identifier: 218235035

GI number: 218235035

Start: 3943486

End: 3944418

Strand: Reverse

Name: mraW [H]

Synonym: BCB4264_A4019

Alternate gene names: 218235035

Gene position: 3944418-3943486 (Counterclockwise)

Preceding gene: 218235759

Following gene: 218235302

Centisome position: 72.79

GC content: 37.08

Gene sequence:

>933_bases
ATGTTTAAACACGTAACAGTGCTTTTAAAGGAAACAGTAGACGGCTTAAATATAAAGCCAGATGGTACATATGTAGATTG
TACACTGGGGGGAGGAGGACATAGTTCTTATTTATTATCCCAATTAACTGAAGGTGGAAAATTAATAGCGTTTGATCAAG
ATGAGATAGCGATTCAAAACGCGAAAGAAAAGTTCTCTTCATACGGTGAACAGTTTATAACAGTAAAGAGTAACTTCCGT
TATTTAGCTGAAAAACTGCAGGAAATAGGTATAACAGAAGTAGACGGTATTTTATTTGATTTAGGTGTTTCATCCCCACA
ATTAGATACACCAGAACGAGGCTTTAGTTATCATCATGATGCACCGCTAGATATGCGGATGGATCAAGATGCCCCATTAA
CAGCGTACGATGTTGTAAATAGCTGGTCATATGAACAACTTGTAAGGATTTTCTTCCAATACGGTGAAGAGAAATTTTCA
AAACAAATTGCAAGAAAAATTGAAGCTTATCGTGAGAATAAAGCTATTGAAACGACAGGAGAATTAGTAGAGCTAATTAA
AGAAGGTATTCCGGCTCCTGCTCGTAGAACGGGTGGGCATCCTGCTAAGCGAGTGTTTCAGGCAATTCGTATAGCTGTAA
ATGATGAATTAAAAGTATTTGAAGAAGCATTGGAATCTGCTATTGAGATGGTTAAGCCAGGTGGAAGGGTTAGTGTTATA
ACATTCCATTCATTAGAAGATCGTATTTGTAAAACAACGTTTAAGCGAAATAGTACAACGCCACAATTGCCGCCAGGATT
ACCGATTATTCCTGATGAATTTAAGCCGAAATTAAAGCTTATTACAAGAAAACCGATTTTACCTTCTGATATAGAGCTAG
AAGAAAATAATCGTGCGCGTTCTGCGAAGTTGAGAATCGCTGAAAAACGATAA

Upstream 100 bases:

>100_bases
AGTCGTTCTTTCGTATTATTTCATTCTCTAAGCGTGAAATATGTGTTTAGAGGAAGGGATACATTCAACATAATTAATTG
GAAAAAGAGTAGGTGCAACA

Downstream 100 bases:

>100_bases
AAGGGAGAGAGAGGTATGACTAATTTAGCTGTAAAGTACAAACAACAAGCGCAAGAAGAGGTGCAAATTCAAACGCCCCC
ACAGCAGATGGCTAAGCCGA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MFKHVTVLLKETVDGLNIKPDGTYVDCTLGGGGHSSYLLSQLTEGGKLIAFDQDEIAIQNAKEKFSSYGEQFITVKSNFR
YLAEKLQEIGITEVDGILFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQDAPLTAYDVVNSWSYEQLVRIFFQYGEEKFS
KQIARKIEAYRENKAIETTGELVELIKEGIPAPARRTGGHPAKRVFQAIRIAVNDELKVFEEALESAIEMVKPGGRVSVI
TFHSLEDRICKTTFKRNSTTPQLPPGLPIIPDEFKPKLKLITRKPILPSDIELEENNRARSAKLRIAEKR

Sequences:

>Translated_310_residues
MFKHVTVLLKETVDGLNIKPDGTYVDCTLGGGGHSSYLLSQLTEGGKLIAFDQDEIAIQNAKEKFSSYGEQFITVKSNFR
YLAEKLQEIGITEVDGILFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQDAPLTAYDVVNSWSYEQLVRIFFQYGEEKFS
KQIARKIEAYRENKAIETTGELVELIKEGIPAPARRTGGHPAKRVFQAIRIAVNDELKVFEEALESAIEMVKPGGRVSVI
TFHSLEDRICKTTFKRNSTTPQLPPGLPIIPDEFKPKLKLITRKPILPSDIELEENNRARSAKLRIAEKR
>Mature_310_residues
MFKHVTVLLKETVDGLNIKPDGTYVDCTLGGGGHSSYLLSQLTEGGKLIAFDQDEIAIQNAKEKFSSYGEQFITVKSNFR
YLAEKLQEIGITEVDGILFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQDAPLTAYDVVNSWSYEQLVRIFFQYGEEKFS
KQIARKIEAYRENKAIETTGELVELIKEGIPAPARRTGGHPAKRVFQAIRIAVNDELKVFEEALESAIEMVKPGGRVSVI
TFHSLEDRICKTTFKRNSTTPQLPPGLPIIPDEFKPKLKLITRKPILPSDIELEENNRARSAKLRIAEKR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=339, Percent_Identity=35.3982300884956, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI165377202, Length=191, Percent_Identity=38.2198952879581, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1786270, Length=312, Percent_Identity=45.8333333333333, Blast_Score=256, Evalue=1e-69,
Organism=Drosophila melanogaster, GI62472493, Length=330, Percent_Identity=37.8787878787879, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI28571637, Length=330, Percent_Identity=37.8787878787879, Blast_Score=172, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 34928; Mature: 34928

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKHVTVLLKETVDGLNIKPDGTYVDCTLGGGGHSSYLLSQLTEGGKLIAFDQDEIAIQN
CCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCHHHHHHH
AKEKFSSYGEQFITVKSNFRYLAEKLQEIGITEVDGILFDLGVSSPQLDTPERGFSYHHD
HHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCHHCCEEEECCCCCCCCCCCCCCCCCCCC
APLDMRMDQDAPLTAYDVVNSWSYEQLVRIFFQYGEEKFSKQIARKIEAYRENKAIETTG
CCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ELVELIKEGIPAPARRTGGHPAKRVFQAIRIAVNDELKVFEEALESAIEMVKPGGRVSVI
HHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEE
TFHSLEDRICKTTFKRNSTTPQLPPGLPIIPDEFKPKLKLITRKPILPSDIELEENNRAR
EECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEECCCCCCC
SAKLRIAEKR
HHEEEEECCC
>Mature Secondary Structure
MFKHVTVLLKETVDGLNIKPDGTYVDCTLGGGGHSSYLLSQLTEGGKLIAFDQDEIAIQN
CCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCHHHHHHH
AKEKFSSYGEQFITVKSNFRYLAEKLQEIGITEVDGILFDLGVSSPQLDTPERGFSYHHD
HHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCHHCCEEEECCCCCCCCCCCCCCCCCCCC
APLDMRMDQDAPLTAYDVVNSWSYEQLVRIFFQYGEEKFSKQIARKIEAYRENKAIETTG
CCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ELVELIKEGIPAPARRTGGHPAKRVFQAIRIAVNDELKVFEEALESAIEMVKPGGRVSVI
HHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEE
TFHSLEDRICKTTFKRNSTTPQLPPGLPIIPDEFKPKLKLITRKPILPSDIELEENNRAR
EECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEECCCCCCC
SAKLRIAEKR
HHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA