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The map label for this gene is potB [H]

Identifier: 218234855

GI number: 218234855

Start: 1296839

End: 1297687

Strand: Direct

Name: potB [H]

Synonym: BCB4264_A1338

Alternate gene names: 218234855

Gene position: 1296839-1297687 (Clockwise)

Preceding gene: 218232537

Following gene: 218231371

Centisome position: 23.93

GC content: 37.34

Gene sequence:

>849_bases
TTGAAAAAAGGGAAATTACTCGCACTACCTACAGTCGCGTGGCTGTTAATTTTCTTCCTAATCCCGCTCGTATTCGTATT
AGGATTCGCCTTCATGCAGCGCGGAGCTTACGGGACAGTAGAAATGCAATTTACGCTGGAGAATATATCCCGTGTATTTG
ATCCATTGTACTTAGGAACGTTATGGGAAACTGTTAAGATCGCAGTTATTACAACAGTAATATGTTTATTAATTGGTTAT
CCGTTTGCGTATACAATTACAATTGTTGACCGCAAATACCGCTCTATTCTTTTATTATTGGCAACGATCCCGTTTTGGAT
TAACTTTCTTGTTCGTTCATACGCATGGATTGTTATTTTACGTTCACAAGGTCTTGTGAATACGTTGTTATTAAAGCTTG
GCATTATTAGTGAACCGTTAAATTTATTATATAATACTCCTTCTGTAATACTCGGAATGGTTTATTCTTTATTACCATTT
ATGATTTTACCAGTGTATGCAGCAATTGAGCAGCTGGATAAGCGTAAGCTAGAAGCGGCTTATGATTTAGGAGCAACACC
AATAAAGGCATTTTGGAATATAACAGTGCCGATGACGATGTCAGGAATTGCGACTGGTTCTATTTTAGTATTCGTTTCTT
CTATCGGAATGTTTGTTGTATCAGACGTGATGGGCGGATCGAAAGTAGCATTAATCGGAAACGTTATTCAAAATCAATTC
TTAGGTGCACGTGACTGGCCGTTTGGATCTGCGTTATCGATTATCGTTGTTCTATTCTCTGTTCTGTTAATTTACTTATA
TTATCGTGCAACGAAAGTATATAAATATAATGAGAACGGAGGGGAATAG

Upstream 100 bases:

>100_bases
AATTACTAATGGCATATCAAACTGCTGAAGAGGCAGCGCAGTGGAGCATTGGAGATAATGTATATGTAGGCTGGGAGCAA
GAGGACGAGGTGACCTTAAA

Downstream 100 bases:

>100_bases
GCAAAGATGAAGAAGTTTTTAGTTTCTTATTCTTGGTTAATTTTGTTATTCTTGTATTTTCCAATGATGGTTTTAATGGT
CTATTCTTTCAACGACTCTC

Product: spermidine/putrescine ABC transporter, permease protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MKKGKLLALPTVAWLLIFFLIPLVFVLGFAFMQRGAYGTVEMQFTLENISRVFDPLYLGTLWETVKIAVITTVICLLIGY
PFAYTITIVDRKYRSILLLLATIPFWINFLVRSYAWIVILRSQGLVNTLLLKLGIISEPLNLLYNTPSVILGMVYSLLPF
MILPVYAAIEQLDKRKLEAAYDLGATPIKAFWNITVPMTMSGIATGSILVFVSSIGMFVVSDVMGGSKVALIGNVIQNQF
LGARDWPFGSALSIIVVLFSVLLIYLYYRATKVYKYNENGGE

Sequences:

>Translated_282_residues
MKKGKLLALPTVAWLLIFFLIPLVFVLGFAFMQRGAYGTVEMQFTLENISRVFDPLYLGTLWETVKIAVITTVICLLIGY
PFAYTITIVDRKYRSILLLLATIPFWINFLVRSYAWIVILRSQGLVNTLLLKLGIISEPLNLLYNTPSVILGMVYSLLPF
MILPVYAAIEQLDKRKLEAAYDLGATPIKAFWNITVPMTMSGIATGSILVFVSSIGMFVVSDVMGGSKVALIGNVIQNQF
LGARDWPFGSALSIIVVLFSVLLIYLYYRATKVYKYNENGGE
>Mature_282_residues
MKKGKLLALPTVAWLLIFFLIPLVFVLGFAFMQRGAYGTVEMQFTLENISRVFDPLYLGTLWETVKIAVITTVICLLIGY
PFAYTITIVDRKYRSILLLLATIPFWINFLVRSYAWIVILRSQGLVNTLLLKLGIISEPLNLLYNTPSVILGMVYSLLPF
MILPVYAAIEQLDKRKLEAAYDLGATPIKAFWNITVPMTMSGIATGSILVFVSSIGMFVVSDVMGGSKVALIGNVIQNQF
LGARDWPFGSALSIIVVLFSVLLIYLYYRATKVYKYNENGGE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=262, Percent_Identity=44.6564885496183, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1787080, Length=246, Percent_Identity=39.8373983739837, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1787713, Length=243, Percent_Identity=31.2757201646091, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31539; Mature: 31539

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGKLLALPTVAWLLIFFLIPLVFVLGFAFMQRGAYGTVEMQFTLENISRVFDPLYLGT
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHH
LWETVKIAVITTVICLLIGYPFAYTITIVDRKYRSILLLLATIPFWINFLVRSYAWIVIL
HHHHHHHHHHHHHHHHHHCCCHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
RSQGLVNTLLLKLGIISEPLNLLYNTPSVILGMVYSLLPFMILPVYAAIEQLDKRKLEAA
ECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDLGATPIKAFWNITVPMTMSGIATGSILVFVSSIGMFVVSDVMGGSKVALIGNVIQNQF
HCCCCCHHHHHHEEEECEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LGARDWPFGSALSIIVVLFSVLLIYLYYRATKVYKYNENGGE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCC
>Mature Secondary Structure
MKKGKLLALPTVAWLLIFFLIPLVFVLGFAFMQRGAYGTVEMQFTLENISRVFDPLYLGT
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHH
LWETVKIAVITTVICLLIGYPFAYTITIVDRKYRSILLLLATIPFWINFLVRSYAWIVIL
HHHHHHHHHHHHHHHHHHCCCHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
RSQGLVNTLLLKLGIISEPLNLLYNTPSVILGMVYSLLPFMILPVYAAIEQLDKRKLEAA
ECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDLGATPIKAFWNITVPMTMSGIATGSILVFVSSIGMFVVSDVMGGSKVALIGNVIQNQF
HCCCCCHHHHHHEEEECEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LGARDWPFGSALSIIVVLFSVLLIYLYYRATKVYKYNENGGE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]