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The map label for this gene is ykwC [H]

Identifier: 218234585

GI number: 218234585

Start: 4056511

End: 4057389

Strand: Reverse

Name: ykwC [H]

Synonym: BCB4264_A4151

Alternate gene names: 218234585

Gene position: 4057389-4056511 (Counterclockwise)

Preceding gene: 218230885

Following gene: 218232757

Centisome position: 74.87

GC content: 35.61

Gene sequence:

>879_bases
ATGGAACATAAAACTTTATCAATAGGTTTCATTGGGATTGGCGTAATGGGAAAAAGTATGGTTTATCACTTAATGCAAGA
TGGTCATAAAGTATATGTATATAATAGAACGAAAGCAAAAACAGATTCTTTAGTGCAAGATGGTGCAAATTGGTGTAATA
CGCCAAAAGAGTTAGTGAAGCAAGTTGATATTGTAATGACAATGGTTGGATATCCACATGATGTAGAAGAAGTGTATTTT
GGTATAGAAGGAATTATAGAACATGCAAAAGAAGGTACGATAGCAATTGACTTTACGACATCTACACCTACTTTAGCAAA
ACGGATTAATGAAGTTGCAAAAAGCAAAAATATATATACGTTAGATGCACCTGTCTCAGGAGGAGATGTTGGTGCGAAAG
AGGCAAAACTCGCAATTATGGTAGGTGGGGAGAAAGAAATATATGATAGATGCTTACCTTTACTTGAAAAGTTAGGAACA
AACATTCAATTACAAGGACCAGCTGGGAGTGGACAACATACAAAAATGTGCAATCAAATTGCGATTGCTTCCAATATGAT
TGGAGTATGTGAAGCTGTTGCTTACGCGAAGAAGGCTGGACTGAATCCAGATAAAGTGTTAGAGAGTATTTCAACAGGGG
CAGCAGGTAGCTGGTCATTAAGTAATTTAGCTCCTCGAATGTTAAAAGGAGACTTTGAGCCAGGATTTTATGTAAAGCAT
TTTATGAAAGATATGAAAATTGCTTTAGAGGAAGCAGAAAAATTACAATTACCAGTCCCAGGCTTAAGTTTGGCGAAAGA
ATTGTATGAAGAGTTAATTAAGGATGGCGAAGAAAATAGTGGAACACAAGTACTATATAAAAAATATATAAGGGGGTAA

Upstream 100 bases:

>100_bases
GTTATTTTATTTTCAAATGTGAAAAATAGTATGGATGATGACTTTAATAAAGAGTTCCGAAAGATGGTAAATGATTTATT
AGAACAAAGGGGATGAGAAA

Downstream 100 bases:

>100_bases
ACGAGGTGGACCTTCAAAAGTTTGATGAGATGATTGATACTGTACAGCGAGCTACTTGTATGCAAATTAATGAAAAACAA
AAAGAAGCTTTTAAACAAAA

Product: 2-hydroxy-3-oxopropionate reductase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MEHKTLSIGFIGIGVMGKSMVYHLMQDGHKVYVYNRTKAKTDSLVQDGANWCNTPKELVKQVDIVMTMVGYPHDVEEVYF
GIEGIIEHAKEGTIAIDFTTSTPTLAKRINEVAKSKNIYTLDAPVSGGDVGAKEAKLAIMVGGEKEIYDRCLPLLEKLGT
NIQLQGPAGSGQHTKMCNQIAIASNMIGVCEAVAYAKKAGLNPDKVLESISTGAAGSWSLSNLAPRMLKGDFEPGFYVKH
FMKDMKIALEEAEKLQLPVPGLSLAKELYEELIKDGEENSGTQVLYKKYIRG

Sequences:

>Translated_292_residues
MEHKTLSIGFIGIGVMGKSMVYHLMQDGHKVYVYNRTKAKTDSLVQDGANWCNTPKELVKQVDIVMTMVGYPHDVEEVYF
GIEGIIEHAKEGTIAIDFTTSTPTLAKRINEVAKSKNIYTLDAPVSGGDVGAKEAKLAIMVGGEKEIYDRCLPLLEKLGT
NIQLQGPAGSGQHTKMCNQIAIASNMIGVCEAVAYAKKAGLNPDKVLESISTGAAGSWSLSNLAPRMLKGDFEPGFYVKH
FMKDMKIALEEAEKLQLPVPGLSLAKELYEELIKDGEENSGTQVLYKKYIRG
>Mature_292_residues
MEHKTLSIGFIGIGVMGKSMVYHLMQDGHKVYVYNRTKAKTDSLVQDGANWCNTPKELVKQVDIVMTMVGYPHDVEEVYF
GIEGIIEHAKEGTIAIDFTTSTPTLAKRINEVAKSKNIYTLDAPVSGGDVGAKEAKLAIMVGGEKEIYDRCLPLLEKLGT
NIQLQGPAGSGQHTKMCNQIAIASNMIGVCEAVAYAKKAGLNPDKVLESISTGAAGSWSLSNLAPRMLKGDFEPGFYVKH
FMKDMKIALEEAEKLQLPVPGLSLAKELYEELIKDGEENSGTQVLYKKYIRG

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=283, Percent_Identity=27.208480565371, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI23308751, Length=277, Percent_Identity=29.6028880866426, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI145693186, Length=289, Percent_Identity=35.2941176470588, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1786719, Length=286, Percent_Identity=35.6643356643357, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1790315, Length=267, Percent_Identity=28.8389513108614, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789092, Length=264, Percent_Identity=25.3787878787879, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17557316, Length=276, Percent_Identity=28.9855072463768, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321695, Length=245, Percent_Identity=26.530612244898, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24655230, Length=287, Percent_Identity=33.7979094076655, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19922568, Length=287, Percent_Identity=33.7979094076655, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI28574115, Length=286, Percent_Identity=25.8741258741259, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31890; Mature: 31890

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHKTLSIGFIGIGVMGKSMVYHLMQDGHKVYVYNRTKAKTDSLVQDGANWCNTPKELVK
CCCCEEEEEEEEHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCHHHCCCHHHHHH
QVDIVMTMVGYPHDVEEVYFGIEGIIEHAKEGTIAIDFTTSTPTLAKRINEVAKSKNIYT
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCEEE
LDAPVSGGDVGAKEAKLAIMVGGEKEIYDRCLPLLEKLGTNIQLQGPAGSGQHTKMCNQI
EECCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHH
AIASNMIGVCEAVAYAKKAGLNPDKVLESISTGAAGSWSLSNLAPRMLKGDFEPGFYVKH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHH
FMKDMKIALEEAEKLQLPVPGLSLAKELYEELIKDGEENSGTQVLYKKYIRG
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MEHKTLSIGFIGIGVMGKSMVYHLMQDGHKVYVYNRTKAKTDSLVQDGANWCNTPKELVK
CCCCEEEEEEEEHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCHHHCCCHHHHHH
QVDIVMTMVGYPHDVEEVYFGIEGIIEHAKEGTIAIDFTTSTPTLAKRINEVAKSKNIYT
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCEEE
LDAPVSGGDVGAKEAKLAIMVGGEKEIYDRCLPLLEKLGTNIQLQGPAGSGQHTKMCNQI
EECCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHH
AIASNMIGVCEAVAYAKKAGLNPDKVLESISTGAAGSWSLSNLAPRMLKGDFEPGFYVKH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHH
FMKDMKIALEEAEKLQLPVPGLSLAKELYEELIKDGEENSGTQVLYKKYIRG
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]