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The map label for this gene is miaB [H]

Identifier: 218234533

GI number: 218234533

Start: 3786204

End: 3787733

Strand: Reverse

Name: miaB [H]

Synonym: BCB4264_A3871

Alternate gene names: 218234533

Gene position: 3787733-3786204 (Counterclockwise)

Preceding gene: 218235402

Following gene: 218233971

Centisome position: 69.9

GC content: 35.95

Gene sequence:

>1530_bases
ATGAACGAGCAACAACGATTAGCAAGTCAACAAGCAAATTCCTCTACGAAAAAAGAAGAGAAAGATTATAGTAAATACTT
TGAAAATGTATATCAACCACCTTCCTTAAAGGATGCGAAAAAGCGCGGGAAAGAAGAAGTTAAAATTGAACGGGATTTTG
GCTTACCTGAAGAGTTTCGCAATTTTGGTACAGGAAGAAAGTTCTATATTCGTACATATGGTTGTCAAATGAATGAGCAT
GATACGGAAGTAATGGCAGGTATTTTCACCGCACTTGGATATGAACCAACTTTTTCAACTGAGGATGCAGATGTAGTTTT
ATTAAACACTTGTGCAATTCGTGAAAATGCTGAAAATAAGGTGTTTGGTGAGCTAGGCCATTTAAAGTCACTCAAACGAA
GAAATCCAGACCTTTTAATTGGTGTATGTGGTTGTATGTCTCAAGAGGAATCTGTTGTAAATAAAATTATGCAAAAAAAT
CAGCATGTAGATATGGTATTTGGAACGCATAATATTCATAGATTACCGTACATTCTAAAAGATGCGATGTTCTCGAAAGA
GACGGTAGTTGAAGTATGGTCAAAAGAAGGTGACGTAATTGAAAACCTTCCGAAAGTACGTCGTGGTGATATTAAAGCTT
GGGTAAATATTATGTATGGCTGTGATAAATTCTGTACGTATTGTATTGTACCGTATACACGCGGAAAAGAGCGAAGTAGA
CGTCCAGAAGATATTATCCAAGAGATTCGTCATTTAGCTGCAAATGGTTATAAAGAAATTACTTTACTTGGTCAAAACGT
AAATGCATATGGTAAAGACTTTGAAGATATAGAATACGGTCTTGGCGATTTAATGGACGAACTTCGTAAAGTTGATATTG
CCCGTATTCGTTTTACAACGAGCCATCCACGTGATTTCGATGATCACTTAATTGAAGTGCTTGGAAAAGGTGGCAACCTA
GTAGAACATATCCATTTACCAGTTCAATCTGGAAGTACGGATATGCTGAAAATTATGGCACGTAAATATTCGCGTGAGCA
TTATTTAGAACTTGTAAGAAAAATTAAAGAAGCAATTCCAGATGTAGTATTAACAACAGATATTATCGTTGGTTTTCCAA
ATGAAACAGATGAGCAATTTGAAGAAACGATGTCGTTATATCGTGAAGTAGGGTTTGATACTGCCTTTACATTCATTTAC
TCTCCTCGCGAAGGTACACCAGCTGCAAAAATGAAAGATAATGTACCGATGGAAGTAAAGAAAGAACGTCTTCAACGCTT
AAATACACTAGTAAATACGTTGGCAATTGAAAAGAATAGTCGTTACAAAGGTCAAATTGTAGAAGTGTTAGTTGATGGGG
AAAGTAAAAATAATCCGGAAGTACTTGCAGGTTATACTCGTACGAATAAACTTGTAAACTTCGTCGCTTCAAAATCTTTA
ATTGGTCAGCTTGTGAAAGTAAAAATAACGGAAGCAAAAACTTGGTCTCTTAACGGGGAATTAGTTGAAGAGCCGATTGA
GGTGAAATAA

Upstream 100 bases:

>100_bases
CAAGTTATCTCTCAAGCTATCTAACCATTTTTGAGGTGAGTCTAGGCGCATTAAAATGGACTATAGAGTATGTATAGTTA
AGGGTGAAAGGAGATTACCG

Downstream 100 bases:

>100_bases
TAGATGAAAGTATATTCGAAAGATGACATTGTTGAACAAGCGAAAGAATTAGCAAAAATGATTTCTGAAACAGAAGAAGT
TGATTTTTTTAAGAAAGCAG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MNEQQRLASQQANSSTKKEEKDYSKYFENVYQPPSLKDAKKRGKEEVKIERDFGLPEEFRNFGTGRKFYIRTYGCQMNEH
DTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELGHLKSLKRRNPDLLIGVCGCMSQEESVVNKIMQKN
QHVDMVFGTHNIHRLPYILKDAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTDMLKIMARKYSREHYLELVRKIKEAIPDVVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIY
SPREGTPAAKMKDNVPMEVKKERLQRLNTLVNTLAIEKNSRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVASKSL
IGQLVKVKITEAKTWSLNGELVEEPIEVK

Sequences:

>Translated_509_residues
MNEQQRLASQQANSSTKKEEKDYSKYFENVYQPPSLKDAKKRGKEEVKIERDFGLPEEFRNFGTGRKFYIRTYGCQMNEH
DTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELGHLKSLKRRNPDLLIGVCGCMSQEESVVNKIMQKN
QHVDMVFGTHNIHRLPYILKDAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTDMLKIMARKYSREHYLELVRKIKEAIPDVVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIY
SPREGTPAAKMKDNVPMEVKKERLQRLNTLVNTLAIEKNSRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVASKSL
IGQLVKVKITEAKTWSLNGELVEEPIEVK
>Mature_509_residues
MNEQQRLASQQANSSTKKEEKDYSKYFENVYQPPSLKDAKKRGKEEVKIERDFGLPEEFRNFGTGRKFYIRTYGCQMNEH
DTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELGHLKSLKRRNPDLLIGVCGCMSQEESVVNKIMQKN
QHVDMVFGTHNIHRLPYILKDAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTDMLKIMARKYSREHYLELVRKIKEAIPDVVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIY
SPREGTPAAKMKDNVPMEVKKERLQRLNTLVNTLAIEKNSRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVASKSL
IGQLVKVKITEAKTWSLNGELVEEPIEVK

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=483, Percent_Identity=37.888198757764, Blast_Score=310, Evalue=3e-84,
Organism=Homo sapiens, GI28872784, Length=483, Percent_Identity=37.888198757764, Blast_Score=309, Evalue=4e-84,
Organism=Homo sapiens, GI93277076, Length=455, Percent_Identity=29.2307692307692, Blast_Score=180, Evalue=3e-45,
Organism=Escherichia coli, GI1786882, Length=440, Percent_Identity=45.2272727272727, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1787057, Length=454, Percent_Identity=26.6519823788546, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=478, Percent_Identity=35.5648535564854, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI71996771, Length=305, Percent_Identity=30.1639344262295, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI21356207, Length=545, Percent_Identity=34.6788990825688, Blast_Score=300, Evalue=2e-81,
Organism=Drosophila melanogaster, GI19922432, Length=457, Percent_Identity=31.2910284463895, Blast_Score=197, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 58350; Mature: 58350

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQQRLASQQANSSTKKEEKDYSKYFENVYQPPSLKDAKKRGKEEVKIERDFGLPEEFR
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHEEEECCCCCCHHHH
NFGTGRKFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENK
HCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCCCCCH
VFGELGHLKSLKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILK
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
DAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
HHHHCHHHHHHHHHCCCCHHHHCHHHHHCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCC
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTT
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEEEC
SHPRDFDDHLIEVLGKGGNLVEHIHLPVQSGSTDMLKIMARKYSREHYLELVRKIKEAIP
CCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCC
DVVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVK
CEEEEEHEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCHHHH
KERLQRLNTLVNTLAIEKNSRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVASKSL
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHH
IGQLVKVKITEAKTWSLNGELVEEPIEVK
HHHHHHEEEECCEEEECCCHHHCCCCCCC
>Mature Secondary Structure
MNEQQRLASQQANSSTKKEEKDYSKYFENVYQPPSLKDAKKRGKEEVKIERDFGLPEEFR
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHEEEECCCCCCHHHH
NFGTGRKFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENK
HCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCCCCCH
VFGELGHLKSLKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILK
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
DAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
HHHHCHHHHHHHHHCCCCHHHHCHHHHHCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCC
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTT
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEEEC
SHPRDFDDHLIEVLGKGGNLVEHIHLPVQSGSTDMLKIMARKYSREHYLELVRKIKEAIP
CCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCC
DVVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVK
CEEEEEHEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCHHHH
KERLQRLNTLVNTLAIEKNSRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVASKSL
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHH
IGQLVKVKITEAKTWSLNGELVEEPIEVK
HHHHHHEEEECCEEEECCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA