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The map label for this gene is ydgK [H]

Identifier: 218234266

GI number: 218234266

Start: 180288

End: 181538

Strand: Direct

Name: ydgK [H]

Synonym: BCB4264_A0206

Alternate gene names: 218234266

Gene position: 180288-181538 (Clockwise)

Preceding gene: 218232239

Following gene: 218233751

Centisome position: 3.33

GC content: 39.97

Gene sequence:

>1251_bases
GTGAATGAAGTTAATCACGAAATATATAAACCTGTAAAGGCAAACAGGTTATGGATGATTCTTGTATTAGGAACACTTAC
GGCGATTGGGCCATTATCTATTGATATGTATTTGCCTTCATTACCGAAGTTAACGGATGACTTGCAAACAGGTGCATCCC
TCGCGCAGCTTACATTAACAGCTTGTTTGCTTGGGCTGTCAGTAGGACAATTATTTGTTGGCTCAATTAGTGATATTTAC
GGAAGACGCAAACCTCTTATTATTGCTCTTATTATTTATGTTGCTTCTTCTTTACTTTGTGCTGTTGCGCCATCTATTTG
GACATTAGTGTTATTGCGCTTCCTACAAGGAGCTTCAGGATCAGCCGGGATTGTTATATCACGTGCGATGGTACGTGATA
TGTATTCAGGTTCTGAAATGACGAAGTTTTTCTCACTGCTTATGTTAGTAAACGGAGCAGCACCTATTTTGGCACCGATT
ATTGGGGGGCAATTATTACAGTTTACAACGTGGCGCGGTGTTTTCATCGTTCTTGGAGCAATTAGCGTATTCATGTTAAT
CTCAGCTATTTTTGTATTACGTGAAACATTACCTCCTGAAGAAAGAGAAACAGGTGGTTTGTCAGGGACTTTGGCGACGT
ACGGAAAATTGTTAAAGGACCGTCTGTTTATGGGATATGCATTGTCACAAGGATTAGTAACAGCGGCAATGTTTGCATAC
ATTTCGGGCTCACCATTTGTGTTGCAGAACATATACGGAGCATCACCACAGCAGTTTAGTTTATTCTTTGCAATCAACGG
TATCGGTATTATTATTGCTAGTCAGGTTACGGGTCGTTTAGCGGGGAAAGTGAATGAGAAGACATTATTTGTCTCTGGTA
TCATTATTGCAGCTGTTGGTGGTTTATCTTTACTACTGACAATCTTACTAGGAATAGGGTTAATTGGTGTTTTATGCTCG
TTATTCCTTGTCGTATCAAGTGTTGGGGTTGTCTCAACAACTGGGTTCTCATTAGCGATGAGAAATCAAAAACAAGCTGC
AGGAACAGCATCAGCTTTATTAGGTTTATTACAGTTCATTTCAGGAGCACTTGTTGCACCGCTAGTAGGTATTGGTGGAA
GTAATACAGCATTACCGATGGGTGTTGTAATAGCTCTTTGTGAAGTTGGTGCGGTACTATGCTATTTATTTATGGCTAGA
AGAAGTGAAAAGCAGTTTGAACTGCAAGCAAGACAAAATTTAGAGGCTTAA

Upstream 100 bases:

>100_bases
TTACATTATGAAAGAGGAAAATGCGCTGTACTTGACGTATGTTTATGATGTAACTATAATTGTTACGATTCAGTTTGTAT
AGGAAAGGGTGGATTGAAAA

Downstream 100 bases:

>100_bases
CAAAAAAGAACTGATTCAAAGGGAATATTTGAATCAGTTCTTTTTTCATTTCTTTGAAATACCTCATATAGTTTTGTAAG
GTGCTGTAGTAAAGCAGTGC

Product: drug resistance transporter, Bcr/CflA family

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MNEVNHEIYKPVKANRLWMILVLGTLTAIGPLSIDMYLPSLPKLTDDLQTGASLAQLTLTACLLGLSVGQLFVGSISDIY
GRRKPLIIALIIYVASSLLCAVAPSIWTLVLLRFLQGASGSAGIVISRAMVRDMYSGSEMTKFFSLLMLVNGAAPILAPI
IGGQLLQFTTWRGVFIVLGAISVFMLISAIFVLRETLPPEERETGGLSGTLATYGKLLKDRLFMGYALSQGLVTAAMFAY
ISGSPFVLQNIYGASPQQFSLFFAINGIGIIIASQVTGRLAGKVNEKTLFVSGIIIAAVGGLSLLLTILLGIGLIGVLCS
LFLVVSSVGVVSTTGFSLAMRNQKQAAGTASALLGLLQFISGALVAPLVGIGGSNTALPMGVVIALCEVGAVLCYLFMAR
RSEKQFELQARQNLEA

Sequences:

>Translated_416_residues
MNEVNHEIYKPVKANRLWMILVLGTLTAIGPLSIDMYLPSLPKLTDDLQTGASLAQLTLTACLLGLSVGQLFVGSISDIY
GRRKPLIIALIIYVASSLLCAVAPSIWTLVLLRFLQGASGSAGIVISRAMVRDMYSGSEMTKFFSLLMLVNGAAPILAPI
IGGQLLQFTTWRGVFIVLGAISVFMLISAIFVLRETLPPEERETGGLSGTLATYGKLLKDRLFMGYALSQGLVTAAMFAY
ISGSPFVLQNIYGASPQQFSLFFAINGIGIIIASQVTGRLAGKVNEKTLFVSGIIIAAVGGLSLLLTILLGIGLIGVLCS
LFLVVSSVGVVSTTGFSLAMRNQKQAAGTASALLGLLQFISGALVAPLVGIGGSNTALPMGVVIALCEVGAVLCYLFMAR
RSEKQFELQARQNLEA
>Mature_416_residues
MNEVNHEIYKPVKANRLWMILVLGTLTAIGPLSIDMYLPSLPKLTDDLQTGASLAQLTLTACLLGLSVGQLFVGSISDIY
GRRKPLIIALIIYVASSLLCAVAPSIWTLVLLRFLQGASGSAGIVISRAMVRDMYSGSEMTKFFSLLMLVNGAAPILAPI
IGGQLLQFTTWRGVFIVLGAISVFMLISAIFVLRETLPPEERETGGLSGTLATYGKLLKDRLFMGYALSQGLVTAAMFAY
ISGSPFVLQNIYGASPQQFSLFFAINGIGIIIASQVTGRLAGKVNEKTLFVSGIIIAAVGGLSLLLTILLGIGLIGVLCS
LFLVVSSVGVVSTTGFSLAMRNQKQAAGTASALLGLLQFISGALVAPLVGIGGSNTALPMGVVIALCEVGAVLCYLFMAR
RSEKQFELQARQNLEA

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=386, Percent_Identity=30.8290155440415, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI48994889, Length=370, Percent_Identity=31.0810810810811, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1790146, Length=378, Percent_Identity=25.1322751322751, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI87082312, Length=381, Percent_Identity=27.5590551181102, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1787065, Length=355, Percent_Identity=23.3802816901408, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790794, Length=331, Percent_Identity=22.9607250755287, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321838, Length=298, Percent_Identity=25.1677852348993, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319657, Length=223, Percent_Identity=26.457399103139, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319517, Length=187, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43919; Mature: 43919

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVNHEIYKPVKANRLWMILVLGTLTAIGPLSIDMYLPSLPKLTDDLQTGASLAQLTLT
CCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEHHCCCCCCHHHHHHHHHHHHHHHHH
ACLLGLSVGQLFVGSISDIYGRRKPLIIALIIYVASSLLCAVAPSIWTLVLLRFLQGASG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SAGIVISRAMVRDMYSGSEMTKFFSLLMLVNGAAPILAPIIGGQLLQFTTWRGVFIVLGA
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
ISVFMLISAIFVLRETLPPEERETGGLSGTLATYGKLLKDRLFMGYALSQGLVTAAMFAY
HHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISGSPFVLQNIYGASPQQFSLFFAINGIGIIIASQVTGRLAGKVNEKTLFVSGIIIAAVG
HCCCCHHHHHHCCCCCCCEEEEEEECCCEEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHH
GLSLLLTILLGIGLIGVLCSLFLVVSSVGVVSTTGFSLAMRNQKQAAGTASALLGLLQFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCHHHHHCCCHHHHHHHHHHHHHHHHH
SGALVAPLVGIGGSNTALPMGVVIALCEVGAVLCYLFMARRSEKQFELQARQNLEA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]