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The map label for this gene is cwlH [H]

Identifier: 218234163

GI number: 218234163

Start: 3159155

End: 3160954

Strand: Reverse

Name: cwlH [H]

Synonym: BCB4264_A3268

Alternate gene names: 218234163

Gene position: 3160954-3159155 (Counterclockwise)

Preceding gene: 218233577

Following gene: 218235097

Centisome position: 58.33

GC content: 37.17

Gene sequence:

>1800_bases
ATGAATAAGACAATCAAACAACAAGTAATCGCACTATCAACAAGTCTAGTGATTTTAGGGAGCGTACCTTTATCAGCATC
AGCAGAAGAGATCAACAAATCTCAAACTGATCAACTTAATATTGCCCAAGGAGAACACGGAACGAGCGGATTAAATTATT
CTAATTTCAAGTTGGAAGTTCCTGATCATGTACAAATTAACGAAGCAGAAGTATCTGGAAATGAAAAAATGCTTTTAGAA
GAATATAAGCATAAACATAATGAAACAGAAGAATTAAACAAAATGCAAGATGCTAAGAAGGGACAAGTAGCAGAAAAGGA
AGCTATTTCAAACATTGAAAAGGCATTTGAATCTGTAGATGGTCGAAATGATGGATCTGATCGTCAAATGAAACCTGTTG
CAAAGCCAAGAGGTATTCAATATAGTGCACAGAGTACGAAGAAATATGGAACACTTGCAGGAGTTCCATTTGTGGAGTGG
ATTGTTCCAGCAGGAAATCCTGATATTCGTCCTGCAAATCCGATGAAAGCACGTTACATTACAATCCATGAGACCGCAAA
TACAGCTGCTGGTGCTAATGCTGAAAATCATGCGAAATATTTATACAAGCAAGCAACTGAAGGCACTTTCCGAACAGCTT
CATGGCATTTTACTGTAGATGATAAACAAATTTATCAACACTTACCAACAAACGAAAATGGTTGGCATGCAGGAGATGGA
GATGGTTCAGGAAACAGGGAGTCTATCGGTATCGAGATTGCGGTTAACCAAGACGGTGATTACAATAAAGCGTTAGAAAA
TGCAAAACGACTTGCAGGTTATTTAATGAACAAAGAAGGAATTGGTGCAGATCATATTTACAAGCATCAACAATGGAGCG
GGAAAAAATGTCCTGACATTTTAATTTCACGTGGTAATTGGGCTGGTTTCGTACAAGGAATTCAGTGGTATGCAAATATA
AATGCGCAGATTGATACGCCTTTACAAGATAAAAATGACTCATTTAATCCAAATGAAAATAATCCAGCTGAACCTAGCAT
GGAACTAGTGGTAAATGGTAGTGGCATCAATGTACGTAGTGATGCGGGTATTGAACATCGTGTTGTACGTAAAGCTTCAA
ACGGAGATCGTTACAAAGTATTAGCCGTTAAAAATGGTTGGTATAAAGTAGGTAATGGAGAATGGATTTTCTATGACCCA
AGTTGGATTAAAATTAACTACAATGTACCAAAGGAAGAAGTACAAAAGCCTAAGGAAGAAGTACAAAAACCAAAAGATGA
TATTACAGGTGGCTGGTTTGAAGGGCATATTCGTAAATTAAACAGTCTAGGTATTATGCAAGGCGAAGGCAATGGTGTAT
TTGCTCCATATCGAAATGTAACGAGAGCGGAGTTTGCGAAATTAATCTCGAATGCTCTTAAACTACCAGAGGGAAATAAG
TCATTTGTAGATATTAATGAGGCACATCCATCACTTCATGACGGTATTAAACGTTGTGCAAGTGCAGGAATTATTAATGG
TCGTGGTGAAGGGATTTTTGATCCTAACTCACCAATTACTCGTGAAGAAGTATCTATTATGATTGACAAAGCATTACGAT
ATAAAGGAATTACAGGTGAGTTAGTAGCATTACCATTTACAGATAAGCATTTAATAACATACAAAGAATCGGTTCAACGA
TTATACAGTCTAAAGGTAGTTAATGGTATTGGAAATAATGAGTTTGATCCTCGAGGCACAGCAACTCGTGGTCAGGCGGC
TGCCTTTATTGTGAAGATGTTAGATTCAATGCAAAAATAA

Upstream 100 bases:

>100_bases
TAGGAGTACATAGTAGATTCATATTTTTAAAAATTAATGTGACAATAAAGGGAGGTTGAGATACATACACGGTGGGAGTG
TGTATGTATTTATTATAATA

Downstream 100 bases:

>100_bases
AAGGAAACTTTAATCAGTGAGGGCCACTCGCATTGCAATCAAGCAGTTTATTCACCAAATTTTGAAATGAGAGTGTTACT
GCCCACAAATAGCAGAAGAA

Product: N-acetylmuramoyl-L-alanine amidase and S-layer protein fusion

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MNKTIKQQVIALSTSLVILGSVPLSASAEEINKSQTDQLNIAQGEHGTSGLNYSNFKLEVPDHVQINEAEVSGNEKMLLE
EYKHKHNETEELNKMQDAKKGQVAEKEAISNIEKAFESVDGRNDGSDRQMKPVAKPRGIQYSAQSTKKYGTLAGVPFVEW
IVPAGNPDIRPANPMKARYITIHETANTAAGANAENHAKYLYKQATEGTFRTASWHFTVDDKQIYQHLPTNENGWHAGDG
DGSGNRESIGIEIAVNQDGDYNKALENAKRLAGYLMNKEGIGADHIYKHQQWSGKKCPDILISRGNWAGFVQGIQWYANI
NAQIDTPLQDKNDSFNPNENNPAEPSMELVVNGSGINVRSDAGIEHRVVRKASNGDRYKVLAVKNGWYKVGNGEWIFYDP
SWIKINYNVPKEEVQKPKEEVQKPKDDITGGWFEGHIRKLNSLGIMQGEGNGVFAPYRNVTRAEFAKLISNALKLPEGNK
SFVDINEAHPSLHDGIKRCASAGIINGRGEGIFDPNSPITREEVSIMIDKALRYKGITGELVALPFTDKHLITYKESVQR
LYSLKVVNGIGNNEFDPRGTATRGQAAAFIVKMLDSMQK

Sequences:

>Translated_599_residues
MNKTIKQQVIALSTSLVILGSVPLSASAEEINKSQTDQLNIAQGEHGTSGLNYSNFKLEVPDHVQINEAEVSGNEKMLLE
EYKHKHNETEELNKMQDAKKGQVAEKEAISNIEKAFESVDGRNDGSDRQMKPVAKPRGIQYSAQSTKKYGTLAGVPFVEW
IVPAGNPDIRPANPMKARYITIHETANTAAGANAENHAKYLYKQATEGTFRTASWHFTVDDKQIYQHLPTNENGWHAGDG
DGSGNRESIGIEIAVNQDGDYNKALENAKRLAGYLMNKEGIGADHIYKHQQWSGKKCPDILISRGNWAGFVQGIQWYANI
NAQIDTPLQDKNDSFNPNENNPAEPSMELVVNGSGINVRSDAGIEHRVVRKASNGDRYKVLAVKNGWYKVGNGEWIFYDP
SWIKINYNVPKEEVQKPKEEVQKPKDDITGGWFEGHIRKLNSLGIMQGEGNGVFAPYRNVTRAEFAKLISNALKLPEGNK
SFVDINEAHPSLHDGIKRCASAGIINGRGEGIFDPNSPITREEVSIMIDKALRYKGITGELVALPFTDKHLITYKESVQR
LYSLKVVNGIGNNEFDPRGTATRGQAAAFIVKMLDSMQK
>Mature_599_residues
MNKTIKQQVIALSTSLVILGSVPLSASAEEINKSQTDQLNIAQGEHGTSGLNYSNFKLEVPDHVQINEAEVSGNEKMLLE
EYKHKHNETEELNKMQDAKKGQVAEKEAISNIEKAFESVDGRNDGSDRQMKPVAKPRGIQYSAQSTKKYGTLAGVPFVEW
IVPAGNPDIRPANPMKARYITIHETANTAAGANAENHAKYLYKQATEGTFRTASWHFTVDDKQIYQHLPTNENGWHAGDG
DGSGNRESIGIEIAVNQDGDYNKALENAKRLAGYLMNKEGIGADHIYKHQQWSGKKCPDILISRGNWAGFVQGIQWYANI
NAQIDTPLQDKNDSFNPNENNPAEPSMELVVNGSGINVRSDAGIEHRVVRKASNGDRYKVLAVKNGWYKVGNGEWIFYDP
SWIKINYNVPKEEVQKPKEEVQKPKDDITGGWFEGHIRKLNSLGIMQGEGNGVFAPYRNVTRAEFAKLISNALKLPEGNK
SFVDINEAHPSLHDGIKRCASAGIINGRGEGIFDPNSPITREEVSIMIDKALRYKGITGELVALPFTDKHLITYKESVQR
LYSLKVVNGIGNNEFDPRGTATRGQAAAFIVKMLDSMQK

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 66388; Mature: 66388

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTIKQQVIALSTSLVILGSVPLSASAEEINKSQTDQLNIAQGEHGTSGLNYSNFKLEV
CCHHHHHHHHHHHCEEEEEECCCCCCCHHHHCCCCCCCEEEECCCCCCCCCCCCCEEEEC
PDHVQINEAEVSGNEKMLLEEYKHKHNETEELNKMQDAKKGQVAEKEAISNIEKAFESVD
CCCEEEEHEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
GRNDGSDRQMKPVAKPRGIQYSAQSTKKYGTLAGVPFVEWIVPAGNPDIRPANPMKARYI
CCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEE
TIHETANTAAGANAENHAKYLYKQATEGTFRTASWHFTVDDKQIYQHLPTNENGWHAGDG
EEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHHCCCCCCCCCCCCC
DGSGNRESIGIEIAVNQDGDYNKALENAKRLAGYLMNKEGIGADHIYKHQQWSGKKCPDI
CCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCHH
LISRGNWAGFVQGIQWYANINAQIDTPLQDKNDSFNPNENNPAEPSMELVVNGSGINVRS
EEECCCHHHHHHHEEEEEECCCEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEC
DAGIEHRVVRKASNGDRYKVLAVKNGWYKVGNGEWIFYDPSWIKINYNVPKEEVQKPKEE
CCCCHHHHHHHCCCCCEEEEEEEECCEEECCCCCEEEECCEEEEEEECCCHHHHHCHHHH
VQKPKDDITGGWFEGHIRKLNSLGIMQGEGNGVFAPYRNVTRAEFAKLISNALKLPEGNK
HHCCHHHCCCCHHHHHHHHHHHCEEEECCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCC
SFVDINEAHPSLHDGIKRCASAGIINGRGEGIFDPNSPITREEVSIMIDKALRYKGITGE
CEEEEHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
LVALPFTDKHLITYKESVQRLYSLKVVNGIGNNEFDPRGTATRGQAAAFIVKMLDSMQK
EEEECCCCCEEEHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKTIKQQVIALSTSLVILGSVPLSASAEEINKSQTDQLNIAQGEHGTSGLNYSNFKLEV
CCHHHHHHHHHHHCEEEEEECCCCCCCHHHHCCCCCCCEEEECCCCCCCCCCCCCEEEEC
PDHVQINEAEVSGNEKMLLEEYKHKHNETEELNKMQDAKKGQVAEKEAISNIEKAFESVD
CCCEEEEHEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
GRNDGSDRQMKPVAKPRGIQYSAQSTKKYGTLAGVPFVEWIVPAGNPDIRPANPMKARYI
CCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEE
TIHETANTAAGANAENHAKYLYKQATEGTFRTASWHFTVDDKQIYQHLPTNENGWHAGDG
EEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHHCCCCCCCCCCCCC
DGSGNRESIGIEIAVNQDGDYNKALENAKRLAGYLMNKEGIGADHIYKHQQWSGKKCPDI
CCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCHH
LISRGNWAGFVQGIQWYANINAQIDTPLQDKNDSFNPNENNPAEPSMELVVNGSGINVRS
EEECCCHHHHHHHEEEEEECCCEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEC
DAGIEHRVVRKASNGDRYKVLAVKNGWYKVGNGEWIFYDPSWIKINYNVPKEEVQKPKEE
CCCCHHHHHHHCCCCCEEEEEEEECCEEECCCCCEEEECCEEEEEEECCCHHHHHCHHHH
VQKPKDDITGGWFEGHIRKLNSLGIMQGEGNGVFAPYRNVTRAEFAKLISNALKLPEGNK
HHCCHHHCCCCHHHHHHHHHHHCEEEECCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCC
SFVDINEAHPSLHDGIKRCASAGIINGRGEGIFDPNSPITREEVSIMIDKALRYKGITGE
CEEEEHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
LVALPFTDKHLITYKESVQRLYSLKVVNGIGNNEFDPRGTATRGQAAAFIVKMLDSMQK
EEEECCCCCEEEHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]