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The map label for this gene is purK [H]

Identifier: 218234070

GI number: 218234070

Start: 309465

End: 310616

Strand: Direct

Name: purK [H]

Synonym: BCB4264_A0335

Alternate gene names: 218234070

Gene position: 309465-310616 (Clockwise)

Preceding gene: 218235413

Following gene: 218234739

Centisome position: 5.71

GC content: 37.07

Gene sequence:

>1152_bases
ATGACGAGAATCATTTTACCTGGAAAAACAATCGGTATTATTGGAGGCGGCCAGCTAGGAAGAATGATGGCATTGGCAGC
TAAGGAGATGGGATATAAAATTGCTGTTTTAGATCCTACAAAGCATTCGCCATGTGCACAAGTTGCTGATATTGAAATTG
TTGCACCATATGATGATTTAAAAGCAATTCAGCATTTAGCAGAGATAAGTGATGTTGTCACATATGAATTTGAGAATATT
GATTATAGATGTTTACAATGGCTTGAAAAACATGCGTACTTACCACAAGGTAGTCAGTTGTTAAATACAACGCAAAATCG
TTTTACAGAAAAGAATGCGATTGAGAAGGCTGGGTTACCAGTAGCAACGTATAGATTGGTTCAAAATCAAGATCAGCTTA
CAGAAGCAATTGCTGGGTTATCATTCCCTTCCGTCTTAAAAACGACGACAGGTGGATATGATGGGAAAGGGCAAGTTGTT
TTAAGAAGTGAAGCTGATGTTGAGGCAGCAAGAAATCTTGTGGATAAAGCAGAATGTATTTTAGAGAAATGGGTGCCTTT
TGAAAAAGAAGTATCAGTTATTGTGATTCGTAGTGTAAGTGGTGAAACAAAAGTGTTTCCAGTAGCAGAAAATATTCATG
TAAATAACATTTTACATGAATCTATCGTTCCAGCTCGCATTACAGAAGAGCTTTCTCAAAAAGCAATTGCGTATGCAAAG
GTACTTGCGGATGAATTAGAACTTGTGGGAACACTAGCTGTAGAGATGTTTGCTACAGATAATGGTGAGATTTATATTAA
TGAATTAGCGCCAAGACCTCACAATTCAGGACACTATACACAGGATGCATGTGAAACGAGTCAATTTGGTCAACATATTC
GAGCAATCTGTAATTTACCTCTAGGAGAAACAAATTTGTTAAAACCAGTTGTCATGGTAAACATTTTAGGCGAACATATA
GAAGGGGTCCTAAGACAAGTGAATAGATTAACCGGGTGCTATTTACACTTGTATGGAAAAGAAGAAGCAAAAGCACAGCG
AAAAATGGGGCATGTTAATATTTTAAATGATAATATTGAAGTCGCTCTAGAAAAAGCGAAGAGTTTGCATATTTGGGACC
ATCAAGAACAACTGTTGGAGGGAAAAAGATGA

Upstream 100 bases:

>100_bases
CTGCACAAATACTTGGATCATTTCATGATGATATACATGATGCATTAGAATTGAGACGAGAAGCGATTGAAAAAGATGTG
CGTGAAGGTAGTGAGCTAGT

Downstream 100 bases:

>100_bases
TTAGTCGTTATACACGCCCTGAAATGGGTGCGATTTGGACGGAAGAGAACAAATTTAAAGCGTGGTTAGAAGTTGAGATT
TTAGCTTGTGAAGCATGGGC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTRIILPGKTIGIIGGGQLGRMMALAAKEMGYKIAVLDPTKHSPCAQVADIEIVAPYDDLKAIQHLAEISDVVTYEFENI
DYRCLQWLEKHAYLPQGSQLLNTTQNRFTEKNAIEKAGLPVATYRLVQNQDQLTEAIAGLSFPSVLKTTTGGYDGKGQVV
LRSEADVEAARNLVDKAECILEKWVPFEKEVSVIVIRSVSGETKVFPVAENIHVNNILHESIVPARITEELSQKAIAYAK
VLADELELVGTLAVEMFATDNGEIYINELAPRPHNSGHYTQDACETSQFGQHIRAICNLPLGETNLLKPVVMVNILGEHI
EGVLRQVNRLTGCYLHLYGKEEAKAQRKMGHVNILNDNIEVALEKAKSLHIWDHQEQLLEGKR

Sequences:

>Translated_383_residues
MTRIILPGKTIGIIGGGQLGRMMALAAKEMGYKIAVLDPTKHSPCAQVADIEIVAPYDDLKAIQHLAEISDVVTYEFENI
DYRCLQWLEKHAYLPQGSQLLNTTQNRFTEKNAIEKAGLPVATYRLVQNQDQLTEAIAGLSFPSVLKTTTGGYDGKGQVV
LRSEADVEAARNLVDKAECILEKWVPFEKEVSVIVIRSVSGETKVFPVAENIHVNNILHESIVPARITEELSQKAIAYAK
VLADELELVGTLAVEMFATDNGEIYINELAPRPHNSGHYTQDACETSQFGQHIRAICNLPLGETNLLKPVVMVNILGEHI
EGVLRQVNRLTGCYLHLYGKEEAKAQRKMGHVNILNDNIEVALEKAKSLHIWDHQEQLLEGKR
>Mature_382_residues
TRIILPGKTIGIIGGGQLGRMMALAAKEMGYKIAVLDPTKHSPCAQVADIEIVAPYDDLKAIQHLAEISDVVTYEFENID
YRCLQWLEKHAYLPQGSQLLNTTQNRFTEKNAIEKAGLPVATYRLVQNQDQLTEAIAGLSFPSVLKTTTGGYDGKGQVVL
RSEADVEAARNLVDKAECILEKWVPFEKEVSVIVIRSVSGETKVFPVAENIHVNNILHESIVPARITEELSQKAIAYAKV
LADELELVGTLAVEMFATDNGEIYINELAPRPHNSGHYTQDACETSQFGQHIRAICNLPLGETNLLKPVVMVNILGEHIE
GVLRQVNRLTGCYLHLYGKEEAKAQRKMGHVNILNDNIEVALEKAKSLHIWDHQEQLLEGKR

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=351, Percent_Identity=31.6239316239316, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1788155, Length=371, Percent_Identity=24.5283018867925, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324702, Length=369, Percent_Identity=34.9593495934959, Blast_Score=216, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 42519; Mature: 42388

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIILPGKTIGIIGGGQLGRMMALAAKEMGYKIAVLDPTKHSPCAQVADIEIVAPYDDL
CCEEEECCCEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHCCEEEECCHHHH
KAIQHLAEISDVVTYEFENIDYRCLQWLEKHAYLPQGSQLLNTTQNRFTEKNAIEKAGLP
HHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
VATYRLVQNQDQLTEAIAGLSFPSVLKTTTGGYDGKGQVVLRSEADVEAARNLVDKAECI
HHHHHHHCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
LEKWVPFEKEVSVIVIRSVSGETKVFPVAENIHVNNILHESIVPARITEELSQKAIAYAK
HHHCCCCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
VLADELELVGTLAVEMFATDNGEIYINELAPRPHNSGHYTQDACETSQFGQHIRAICNLP
HHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
LGETNLLKPVVMVNILGEHIEGVLRQVNRLTGCYLHLYGKEEAKAQRKMGHVNILNDNIE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHCCCEEEECCCHH
VALEKAKSLHIWDHQEQLLEGKR
HHHHHHCCCEEECCHHHHHCCCC
>Mature Secondary Structure 
TRIILPGKTIGIIGGGQLGRMMALAAKEMGYKIAVLDPTKHSPCAQVADIEIVAPYDDL
CEEEECCCEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHCCEEEECCHHHH
KAIQHLAEISDVVTYEFENIDYRCLQWLEKHAYLPQGSQLLNTTQNRFTEKNAIEKAGLP
HHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
VATYRLVQNQDQLTEAIAGLSFPSVLKTTTGGYDGKGQVVLRSEADVEAARNLVDKAECI
HHHHHHHCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
LEKWVPFEKEVSVIVIRSVSGETKVFPVAENIHVNNILHESIVPARITEELSQKAIAYAK
HHHCCCCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
VLADELELVGTLAVEMFATDNGEIYINELAPRPHNSGHYTQDACETSQFGQHIRAICNLP
HHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
LGETNLLKPVVMVNILGEHIEGVLRQVNRLTGCYLHLYGKEEAKAQRKMGHVNILNDNIE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHCCCEEEECCCHH
VALEKAKSLHIWDHQEQLLEGKR
HHHHHHCCCEEECCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]