Click here to switch to the map view.

The map label for this gene is glsA2 [H]

Identifier: 218233997

GI number: 218233997

Start: 3020295

End: 3021275

Strand: Reverse

Name: glsA2 [H]

Synonym: BCB4264_A3142

Alternate gene names: 218233997

Gene position: 3021275-3020295 (Counterclockwise)

Preceding gene: 218231616

Following gene: 218233604

Centisome position: 55.75

GC content: 36.9

Gene sequence:

>981_bases
ATGATAAAGGATTCTAGCGTTCAAGTTGAGGGTCAAGAAAAAGTTTGCTTAGATCAATGGGTAGCTCATTATCGAACGTA
TGCAGCTAAGGGACGAAGCGCCAGTTATATTCCCGCTCTGGGGGAAATAAATGTATCGCAGTTAGGTATTTGTATAGTAA
AGCCAGATGGAACAATGATAAAATCAGGAGATTGGGAGATACCTTTCACATTACAAAGTATATCAAAAGTAATCGGTTTT
ATAGCCGCTTGTTTAAGTCGCGGTATTTCTTATGTATTAGAACGAGTTGACGTAGAGCCAACTGGAGATGCCTTTAATTC
AATTATTCGTTTAGAGATACATAAACCAGGAAAACCTTTTAATCCTATGATTAATGCTGGAGCGATTACGATAGCTTCAC
TTTTGCCAGGGACATCGGTGCAAGAAAAATTAGAATCTCTATACGTATTAATTGAAAAAATGATAGAGAAGCGTCCCGCT
ATTAATGAAATAGTATTTCAATCAGAATGGGAAACGGCTCATCGTAATAGAGCATTAGCATACTATTTAAAGGAGAACGG
TTTTCTAGAATCAGATGTGGAGGAGACACTAGAGGTTTACCTAAAACAATGTTCGATTGAAATAAATACAGAAGATATTG
CCTTAATAGGGCTTATACTAGCACATGATGGATATCATCCTATTCGCAAAGAACAAGTGCTCCCTAAAGAAGTAGCTAGG
TTAACAAAAGCTTTAATGCTAACTTGTGGAATGTACAATGCTTCTGGAAAATTTGCTGCTTTCATTGGATTACCAGCTAA
GAGTGGGGTATCGGGCGGAATTATGACACTTGTTCCCTCAAAATCTAGGAAGGATTTATCGTTCCAAGATGGATGTGGTA
TTGGTATTTACGGACCAGCCATTGATGAATATGGGAATAGTTTACCAGGAATTATGTTGTTAGAACATATTGCAAAAGAA
TGGGATTTAAGTATATTTTAA

Upstream 100 bases:

>100_bases
ATGTCTGAATATTTTTCTTGGTTCTAGTGCAAATATTATTGATTATAGTAAAGAAAAAATCATTCTATATAAAGTTAATA
GTACGGGGGAATATGATTAA

Downstream 100 bases:

>100_bases
AAAGGAGCACAGATATTTTGCTGTTAGAGCGAGTCCAAAAGTGTTCAGACCTTATATTGTGTCTGAACATTTTTGAGTTA
TAAAGTATCTTTCTAAAGTA

Product: glutaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MIKDSSVQVEGQEKVCLDQWVAHYRTYAAKGRSASYIPALGEINVSQLGICIVKPDGTMIKSGDWEIPFTLQSISKVIGF
IAACLSRGISYVLERVDVEPTGDAFNSIIRLEIHKPGKPFNPMINAGAITIASLLPGTSVQEKLESLYVLIEKMIEKRPA
INEIVFQSEWETAHRNRALAYYLKENGFLESDVEETLEVYLKQCSIEINTEDIALIGLILAHDGYHPIRKEQVLPKEVAR
LTKALMLTCGMYNASGKFAAFIGLPAKSGVSGGIMTLVPSKSRKDLSFQDGCGIGIYGPAIDEYGNSLPGIMLLEHIAKE
WDLSIF

Sequences:

>Translated_326_residues
MIKDSSVQVEGQEKVCLDQWVAHYRTYAAKGRSASYIPALGEINVSQLGICIVKPDGTMIKSGDWEIPFTLQSISKVIGF
IAACLSRGISYVLERVDVEPTGDAFNSIIRLEIHKPGKPFNPMINAGAITIASLLPGTSVQEKLESLYVLIEKMIEKRPA
INEIVFQSEWETAHRNRALAYYLKENGFLESDVEETLEVYLKQCSIEINTEDIALIGLILAHDGYHPIRKEQVLPKEVAR
LTKALMLTCGMYNASGKFAAFIGLPAKSGVSGGIMTLVPSKSRKDLSFQDGCGIGIYGPAIDEYGNSLPGIMLLEHIAKE
WDLSIF
>Mature_326_residues
MIKDSSVQVEGQEKVCLDQWVAHYRTYAAKGRSASYIPALGEINVSQLGICIVKPDGTMIKSGDWEIPFTLQSISKVIGF
IAACLSRGISYVLERVDVEPTGDAFNSIIRLEIHKPGKPFNPMINAGAITIASLLPGTSVQEKLESLYVLIEKMIEKRPA
INEIVFQSEWETAHRNRALAYYLKENGFLESDVEETLEVYLKQCSIEINTEDIALIGLILAHDGYHPIRKEQVLPKEVAR
LTKALMLTCGMYNASGKFAAFIGLPAKSGVSGGIMTLVPSKSRKDLSFQDGCGIGIYGPAIDEYGNSLPGIMLLEHIAKE
WDLSIF

Specific function: Unknown

COG id: COG2066

COG function: function code E; Glutaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutaminase family [H]

Homologues:

Organism=Homo sapiens, GI156104878, Length=311, Percent_Identity=36.9774919614148, Blast_Score=184, Evalue=8e-47,
Organism=Homo sapiens, GI20336214, Length=297, Percent_Identity=35.016835016835, Blast_Score=169, Evalue=3e-42,
Organism=Escherichia coli, GI1786693, Length=296, Percent_Identity=35.472972972973, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1787804, Length=314, Percent_Identity=34.0764331210191, Blast_Score=184, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17507019, Length=301, Percent_Identity=34.21926910299, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17532727, Length=299, Percent_Identity=32.7759197324415, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI193204073, Length=308, Percent_Identity=32.4675324675325, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI193204075, Length=310, Percent_Identity=32.9032258064516, Blast_Score=157, Evalue=8e-39,
Organism=Drosophila melanogaster, GI281363239, Length=304, Percent_Identity=34.5394736842105, Blast_Score=174, Evalue=7e-44,
Organism=Drosophila melanogaster, GI281363241, Length=301, Percent_Identity=34.8837209302326, Blast_Score=174, Evalue=7e-44,
Organism=Drosophila melanogaster, GI24653158, Length=304, Percent_Identity=34.5394736842105, Blast_Score=174, Evalue=8e-44,
Organism=Drosophila melanogaster, GI24653162, Length=301, Percent_Identity=34.8837209302326, Blast_Score=174, Evalue=8e-44,
Organism=Drosophila melanogaster, GI116008307, Length=304, Percent_Identity=34.5394736842105, Blast_Score=174, Evalue=8e-44,
Organism=Drosophila melanogaster, GI24653156, Length=304, Percent_Identity=34.5394736842105, Blast_Score=174, Evalue=8e-44,
Organism=Drosophila melanogaster, GI24653164, Length=304, Percent_Identity=34.5394736842105, Blast_Score=174, Evalue=9e-44,
Organism=Drosophila melanogaster, GI24653166, Length=304, Percent_Identity=34.5394736842105, Blast_Score=173, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015868 [H]

Pfam domain/function: PF04960 Glutaminase [H]

EC number: =3.5.1.2 [H]

Molecular weight: Translated: 35886; Mature: 35886

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKDSSVQVEGQEKVCLDQWVAHYRTYAAKGRSASYIPALGEINVSQLGICIVKPDGTMI
CCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEECCCCCEE
KSGDWEIPFTLQSISKVIGFIAACLSRGISYVLERVDVEPTGDAFNSIIRLEIHKPGKPF
ECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEECCCCCC
NPMINAGAITIASLLPGTSVQEKLESLYVLIEKMIEKRPAINEIVFQSEWETAHRNRALA
CCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHCCCEEE
YYLKENGFLESDVEETLEVYLKQCSIEINTEDIALIGLILAHDGYHPIRKEQVLPKEVAR
EEEECCCCCHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCCCCHHHHCCCHHHHHH
LTKALMLTCGMYNASGKFAAFIGLPAKSGVSGGIMTLVPSKSRKDLSFQDGCGIGIYGPA
HHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEECCC
IDEYGNSLPGIMLLEHIAKEWDLSIF
HHHHCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIKDSSVQVEGQEKVCLDQWVAHYRTYAAKGRSASYIPALGEINVSQLGICIVKPDGTMI
CCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEECCCCCEE
KSGDWEIPFTLQSISKVIGFIAACLSRGISYVLERVDVEPTGDAFNSIIRLEIHKPGKPF
ECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEECCCCCC
NPMINAGAITIASLLPGTSVQEKLESLYVLIEKMIEKRPAINEIVFQSEWETAHRNRALA
CCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHCCCEEE
YYLKENGFLESDVEETLEVYLKQCSIEINTEDIALIGLILAHDGYHPIRKEQVLPKEVAR
EEEECCCCCHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCCCCHHHHCCCHHHHHH
LTKALMLTCGMYNASGKFAAFIGLPAKSGVSGGIMTLVPSKSRKDLSFQDGCGIGIYGPA
HHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEECCC
IDEYGNSLPGIMLLEHIAKEWDLSIF
HHHHCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]