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The map label for this gene is citS [H]

Identifier: 218233979

GI number: 218233979

Start: 938751

End: 940340

Strand: Direct

Name: citS [H]

Synonym: BCB4264_A0965

Alternate gene names: 218233979

Gene position: 938751-940340 (Clockwise)

Preceding gene: 218235014

Following gene: 218231398

Centisome position: 17.32

GC content: 36.86

Gene sequence:

>1590_bases
ATGAAGAAATCACGCAAAGTGTCATTGCAAACGAAAATAGTAAGCTTAATTATCGCGTTAATTTTATTTGTCGTTCTCCT
ATTAGCGGGAATATTTGTTTACATTCAGTCCGTTGATACGAAGCGCCAAGTAGAACAGTTAGCACTGCAAACAGCGAAGT
CGCTTTCTTTTATGCCTGCTATAAAAGATACTTTTCAAAATAACGAGCATAAAAGTACGATTCAATCTATTGCGGAACAA
GTTCGTGAGCAAGCTGGTGCGGATTATGTCATTATAGAAGATCGCTTTGGGGTTATGTATTCCCATTCTAATTCGGAGTT
AATTGGCACAAAGAGTAACAACCCGTATAACTATGAAGCGCTCACTTTTGGCGGATACTATACGCTCGAAGGAAAGGGTT
CAAGTGGCCCGGCTTTAATGGCGAAGGCACCTATTATTGTTCATAACGGAGATTACGATCAAGTAGTAGGCGTTGTGACA
GTAGAGTTTTTAATAAAAGGTATTGAATCTAACATATTGAGTAGAACGAAAGAAATTATCCTCTTTTCATTAGCAGTATT
ATTAGCCGGCATTGTTGGTGGCATTCTTTTAGCACGTAGCATTCGTAAAGATACACTCGGTTTAGAGCCGAATGAAATCG
CAGCGTTATACCGGGAACGAAGCGCAATACTACTATCTATAAAAGAAGGAATTATCGCTATCGATCAAAATGGTTTTATT
ACGATGATGAACACGTCGGCAGAAGAAATGTTACATGTAAATGGTGATTATATGCAGCAGCACATTTCAAAAGTTTTACC
GGAATTTAATATGGAGAGAGTGTTAGAAAGCGATCAAGAAATCGCGTTTCAAGATAAAGTGTTTATTTTAAACATGACAC
CGATACTCGAAAATAATAGTACGGTAGGTGTTGTATGTAGTTTTAGAGATAAAACAGAACTGCAAAACCTTGTGAACACA
ATATCTGAGGTAAGAAAGTATTCAGAAGACTTACGTGCTCAAACACATGAATTTACAAATAAACTTTTTGTCTTATCGGG
ATTGCTTCAGTTAGGGCACTACAAAGAAGCAATTGAATTTATCCAGCAAGAATCTAACATTCATCAAAGCCAAAACCATA
TTTTATTCCATCAAATTCATGATGCGAAAGTACAAGCAATTTTATTAGGAAAACTCGCAACAGCGTCTGAGAGGAAAATT
GATTTTCATATTGAAGGAGATAGTGCGCTTCATCCATTACCAGAACATATAAAAGTTTCGCACCTTATTACGATCCTTGG
AAATATAATCGATAATGCATTTGATGCGGTGAGTGAACGAGGGGAGAAGAATGTTTCTTTCTTTGTTACGGATATTGGGC
ACGATATTGTGTTTGAAGTGATAGATAGTGGAGCAGGGATACCAGTTGAAAAAATCACGACCCTTTTTCAAAAAGGATTT
TCAACGAAAGGGAATGATCGTGGTTATGGACTAGCAAACGTAAAAGAAATGGTAGATCTACTAGGGGGAACAATTGAAAT
TCAAAACGAGAAAAATGGGGGAGCTATTTTTACAATTTACCTTCCGAAAATATTGAGAGAAAGTACATGA

Upstream 100 bases:

>100_bases
GTACATGGATATTTTTAATAGTTGCTTCATTTTCAGTTTTGTCATAATAATAGAAAAAGGAGATTGATAGTTTTGAATAT
TTTATCAAAGGATATGAAAT

Downstream 100 bases:

>100_bases
CAAACAGGGGGATGGGCATATGTTGAAGGTTGCAATTGCAGAAGATGATTTCCGCGTCGCGCAAATTCAAGAGGAGTTTT
TATTAAAAATAAAGGATGTA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MKKSRKVSLQTKIVSLIIALILFVVLLLAGIFVYIQSVDTKRQVEQLALQTAKSLSFMPAIKDTFQNNEHKSTIQSIAEQ
VREQAGADYVIIEDRFGVMYSHSNSELIGTKSNNPYNYEALTFGGYYTLEGKGSSGPALMAKAPIIVHNGDYDQVVGVVT
VEFLIKGIESNILSRTKEIILFSLAVLLAGIVGGILLARSIRKDTLGLEPNEIAALYRERSAILLSIKEGIIAIDQNGFI
TMMNTSAEEMLHVNGDYMQQHISKVLPEFNMERVLESDQEIAFQDKVFILNMTPILENNSTVGVVCSFRDKTELQNLVNT
ISEVRKYSEDLRAQTHEFTNKLFVLSGLLQLGHYKEAIEFIQQESNIHQSQNHILFHQIHDAKVQAILLGKLATASERKI
DFHIEGDSALHPLPEHIKVSHLITILGNIIDNAFDAVSERGEKNVSFFVTDIGHDIVFEVIDSGAGIPVEKITTLFQKGF
STKGNDRGYGLANVKEMVDLLGGTIEIQNEKNGGAIFTIYLPKILREST

Sequences:

>Translated_529_residues
MKKSRKVSLQTKIVSLIIALILFVVLLLAGIFVYIQSVDTKRQVEQLALQTAKSLSFMPAIKDTFQNNEHKSTIQSIAEQ
VREQAGADYVIIEDRFGVMYSHSNSELIGTKSNNPYNYEALTFGGYYTLEGKGSSGPALMAKAPIIVHNGDYDQVVGVVT
VEFLIKGIESNILSRTKEIILFSLAVLLAGIVGGILLARSIRKDTLGLEPNEIAALYRERSAILLSIKEGIIAIDQNGFI
TMMNTSAEEMLHVNGDYMQQHISKVLPEFNMERVLESDQEIAFQDKVFILNMTPILENNSTVGVVCSFRDKTELQNLVNT
ISEVRKYSEDLRAQTHEFTNKLFVLSGLLQLGHYKEAIEFIQQESNIHQSQNHILFHQIHDAKVQAILLGKLATASERKI
DFHIEGDSALHPLPEHIKVSHLITILGNIIDNAFDAVSERGEKNVSFFVTDIGHDIVFEVIDSGAGIPVEKITTLFQKGF
STKGNDRGYGLANVKEMVDLLGGTIEIQNEKNGGAIFTIYLPKILREST
>Mature_529_residues
MKKSRKVSLQTKIVSLIIALILFVVLLLAGIFVYIQSVDTKRQVEQLALQTAKSLSFMPAIKDTFQNNEHKSTIQSIAEQ
VREQAGADYVIIEDRFGVMYSHSNSELIGTKSNNPYNYEALTFGGYYTLEGKGSSGPALMAKAPIIVHNGDYDQVVGVVT
VEFLIKGIESNILSRTKEIILFSLAVLLAGIVGGILLARSIRKDTLGLEPNEIAALYRERSAILLSIKEGIIAIDQNGFI
TMMNTSAEEMLHVNGDYMQQHISKVLPEFNMERVLESDQEIAFQDKVFILNMTPILENNSTVGVVCSFRDKTELQNLVNT
ISEVRKYSEDLRAQTHEFTNKLFVLSGLLQLGHYKEAIEFIQQESNIHQSQNHILFHQIHDAKVQAILLGKLATASERKI
DFHIEGDSALHPLPEHIKVSHLITILGNIIDNAFDAVSERGEKNVSFFVTDIGHDIVFEVIDSGAGIPVEKITTLFQKGF
STKGNDRGYGLANVKEMVDLLGGTIEIQNEKNGGAIFTIYLPKILREST

Specific function: Member of the two-component regulatory system CitT/CitS. Regulates the expression of the citM-yflN operon. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=534, Percent_Identity=31.0861423220974, Blast_Score=247, Evalue=2e-66,
Organism=Escherichia coli, GI1786837, Length=540, Percent_Identity=30.7407407407407, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1790436, Length=129, Percent_Identity=31.0077519379845, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788549, Length=362, Percent_Identity=24.8618784530387, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58810; Mature: 58810

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSRKVSLQTKIVSLIIALILFVVLLLAGIFVYIQSVDTKRQVEQLALQTAKSLSFMPA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IKDTFQNNEHKSTIQSIAEQVREQAGADYVIIEDRFGVMYSHSNSELIGTKSNNPYNYEA
HHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCEEEECCCCCCCEEE
LTFGGYYTLEGKGSSGPALMAKAPIIVHNGDYDQVVGVVTVEFLIKGIESNILSRTKEII
EEECCEEEEECCCCCCCEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFSLAVLLAGIVGGILLARSIRKDTLGLEPNEIAALYRERSAILLSIKEGIIAIDQNGFI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCEEEEECCCEE
TMMNTSAEEMLHVNGDYMQQHISKVLPEFNMERVLESDQEIAFQDKVFILNMTPILENNS
EEECCCHHHHEECCHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCEEEEEECEEECCCC
TVGVVCSFRDKTELQNLVNTISEVRKYSEDLRAQTHEFTNKLFVLSGLLQLGHYKEAIEF
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQQESNIHQSQNHILFHQIHDAKVQAILLGKLATASERKIDFHIEGDSALHPLPEHIKVS
HHHHHCCHHCCCCEEEEEECCHHHHHHEEHHHCCCCCCEEEEEECCCCCCCCCHHHHHHH
HLITILGNIIDNAFDAVSERGEKNVSFFVTDIGHDIVFEVIDSGAGIPVEKITTLFQKGF
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHCC
STKGNDRGYGLANVKEMVDLLGGTIEIQNEKNGGAIFTIYLPKILREST
CCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEHHHHCCCC
>Mature Secondary Structure
MKKSRKVSLQTKIVSLIIALILFVVLLLAGIFVYIQSVDTKRQVEQLALQTAKSLSFMPA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IKDTFQNNEHKSTIQSIAEQVREQAGADYVIIEDRFGVMYSHSNSELIGTKSNNPYNYEA
HHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCEEEECCCCCCCEEE
LTFGGYYTLEGKGSSGPALMAKAPIIVHNGDYDQVVGVVTVEFLIKGIESNILSRTKEII
EEECCEEEEECCCCCCCEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFSLAVLLAGIVGGILLARSIRKDTLGLEPNEIAALYRERSAILLSIKEGIIAIDQNGFI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCEEEEECCCEE
TMMNTSAEEMLHVNGDYMQQHISKVLPEFNMERVLESDQEIAFQDKVFILNMTPILENNS
EEECCCHHHHEECCHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCEEEEEECEEECCCC
TVGVVCSFRDKTELQNLVNTISEVRKYSEDLRAQTHEFTNKLFVLSGLLQLGHYKEAIEF
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQQESNIHQSQNHILFHQIHDAKVQAILLGKLATASERKIDFHIEGDSALHPLPEHIKVS
HHHHHCCHHCCCCEEEEEECCHHHHHHEEHHHCCCCCCEEEEEECCCCCCCCCHHHHHHH
HLITILGNIIDNAFDAVSERGEKNVSFFVTDIGHDIVFEVIDSGAGIPVEKITTLFQKGF
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHCC
STKGNDRGYGLANVKEMVDLLGGTIEIQNEKNGGAIFTIYLPKILREST
CCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 10972810 [H]