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The map label for this gene is hmp [H]

Identifier: 218233952

GI number: 218233952

Start: 1438700

End: 1439908

Strand: Direct

Name: hmp [H]

Synonym: BCB4264_A1504

Alternate gene names: 218233952

Gene position: 1438700-1439908 (Clockwise)

Preceding gene: 218231823

Following gene: 218233517

Centisome position: 26.55

GC content: 34.08

Gene sequence:

>1209_bases
ATGTTAAGTGCAAAAACAATTGAAATCGTAAAGTCAACAGTACCATTATTACAAGAAAAAGGCGTTGAAATTACAACGAG
ATTTTATGAAATTTTATTTTCGGAACATCCGGAGTTATTGAATATTTTCAACCATACAAATCAGAAAAAGGGAAGACAAC
AACAAGCGTTAGCGAATGCTGTTTATGCAGCTGCAACGTACATTGATAATTTAGAAGCTATTATTCCAGTTGTAAAACAA
ATTGGTCATAAGCATAGAAGTTTAGGTATTAAAGCAGAGCATTACCCGATTGTAGGTACATGTTTACTACGTGCCATTAA
AGAGATCGCAGGTGCACCTGATGAAGTTTTAAATGCATGGGGAGAAGCATATGGTGTCATTGCTGATGCATTCATTAGCA
TTGAAGCAGAGATGTATGAAGAAGCTGCACATAAAGAAGGTGGATGGAAAGACTTCCGCAACTTTGTGATTGTTAAAAAA
GTGAAGGAAAGCGATGTTATTACGTCATTTTATTTGAAACCTGAAGATGGAGGGAAAGTTTCTTCATTCATCCCAGGTCA
ATATGTAACGATTCAAATCAATATTGAAGGTGAGACATATACACATAATCGTCAATATAGTTTATCCGATGCTCCTGGGA
AAGAATATTATCGTATTAGTGTAAAAAAAGAAAAAGGTGTAGATACACCAGACGGTAAAGTGTCTAATTACTTACATGAA
CATGTAAAAGAAGGAGATGTTTTACCAGTAAGTGCACCAGCGGGAGATTTCGTGTTAAATATGGATTCAACATTACCAGT
TGTACTAATTAGTGGTGGAGTAGGGATTACACCGATGATGAGTATGTTAAATACGTTAATTGAACAAGATTCAAAACGTA
ATGTATATTTTGTTCATGCAGCAATAAATAGTAATACACATGCAATGAAAGAACACGTTAAGACAGTAGAAAATGAATAT
GAACAAGTTAAAGCATATACTTGTTATTCTGCACCGACTGAAAAAGATTTAGAAATGAAGAACTTTGATAAAGAAGGTTT
CATTGAAAGTGAATGGTTAAAAACTATTATTCCGACAACTGAAGCAGAGTTTTATTTCTGTGGTCCAGTAGCATTTATGA
AGCATATAAATGCTGCACTAACTGATTTAAGTGTGAAACAAGAGCATATTCATTATGAATTTTTCGGCCCAGCAACAAGC
TTACAATAA

Upstream 100 bases:

>100_bases
ATTCACAAAAACACCGTGTTAATAGTGATTAAAATCACAATCTGCATTATAAGATTCAGTTAAACTAAAAACAGTTAAGG
AAATAGAGGAGTGGGATACA

Downstream 100 bases:

>100_bases
GCAAAAAAACGAGGTATTTATTTTACCTCGTTTTTTATTTGTTCTCGTAATAAACGGTTTACTGTTTCACGACGTATACC
TATCAGTTGACCAATTTCGG

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MLSAKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANAVYAAATYIDNLEAIIPVVKQ
IGHKHRSLGIKAEHYPIVGTCLLRAIKEIAGAPDEVLNAWGEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVIVKK
VKESDVITSFYLKPEDGGKVSSFIPGQYVTIQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHE
HVKEGDVLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVYFVHAAINSNTHAMKEHVKTVENEY
EQVKAYTCYSAPTEKDLEMKNFDKEGFIESEWLKTIIPTTEAEFYFCGPVAFMKHINAALTDLSVKQEHIHYEFFGPATS
LQ

Sequences:

>Translated_402_residues
MLSAKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANAVYAAATYIDNLEAIIPVVKQ
IGHKHRSLGIKAEHYPIVGTCLLRAIKEIAGAPDEVLNAWGEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVIVKK
VKESDVITSFYLKPEDGGKVSSFIPGQYVTIQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHE
HVKEGDVLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVYFVHAAINSNTHAMKEHVKTVENEY
EQVKAYTCYSAPTEKDLEMKNFDKEGFIESEWLKTIIPTTEAEFYFCGPVAFMKHINAALTDLSVKQEHIHYEFFGPATS
LQ
>Mature_402_residues
MLSAKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANAVYAAATYIDNLEAIIPVVKQ
IGHKHRSLGIKAEHYPIVGTCLLRAIKEIAGAPDEVLNAWGEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVIVKK
VKESDVITSFYLKPEDGGKVSSFIPGQYVTIQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHE
HVKEGDVLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVYFVHAAINSNTHAMKEHVKTVENEY
EQVKAYTCYSAPTEKDLEMKNFDKEGFIESEWLKTIIPTTEAEFYFCGPVAFMKHINAALTDLSVKQEHIHYEFFGPATS
LQ

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=397, Percent_Identity=46.3476070528967, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1787658, Length=259, Percent_Identity=25.8687258687259, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788104, Length=249, Percent_Identity=26.1044176706827, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321673, Length=413, Percent_Identity=42.6150121065375, Blast_Score=281, Evalue=2e-76,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 45062; Mature: 45062

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANA
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHCCHHHHHHHHH
VYAAATYIDNLEAIIPVVKQIGHKHRSLGIKAEHYPIVGTCLLRAIKEIAGAPDEVLNAW
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
GEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVIVKKVKESDVITSFYLKPEDGGKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEECCCCCCEEEEEECCCCCCCC
SSFIPGQYVTIQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHE
CCCCCCEEEEEEEEECCCEEECCCEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
HVKEGDVLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVYFVHA
HHCCCCEEEEECCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEE
AINSNTHAMKEHVKTVENEYEQVKAYTCYSAPTEKDLEMKNFDKEGFIESEWLKTIIPTT
EECCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHCCCCCCCCHHHHHHHHCCCC
EAEFYFCGPVAFMKHINAALTDLSVKQEHIHYEFFGPATSLQ
CCCEEEECHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCC
>Mature Secondary Structure
MLSAKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANA
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHCCHHHHHHHHH
VYAAATYIDNLEAIIPVVKQIGHKHRSLGIKAEHYPIVGTCLLRAIKEIAGAPDEVLNAW
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
GEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVIVKKVKESDVITSFYLKPEDGGKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEECCCCCCEEEEEECCCCCCCC
SSFIPGQYVTIQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHE
CCCCCCEEEEEEEEECCCEEECCCEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
HVKEGDVLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVYFVHA
HHCCCCEEEEECCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEE
AINSNTHAMKEHVKTVENEYEQVKAYTCYSAPTEKDLEMKNFDKEGFIESEWLKTIIPTT
EECCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHCCCCCCCCHHHHHHHHCCCC
EAEFYFCGPVAFMKHINAALTDLSVKQEHIHYEFFGPATSLQ
CCCEEEECHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]