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The map label for this gene is fliM [H]

Identifier: 218233573

GI number: 218233573

Start: 1644388

End: 1645374

Strand: Direct

Name: fliM [H]

Synonym: BCB4264_A1729

Alternate gene names: 218233573

Gene position: 1644388-1645374 (Clockwise)

Preceding gene: 218235179

Following gene: 218234143

Centisome position: 30.34

GC content: 36.78

Gene sequence:

>987_bases
ATGAGTGGCGAAAAATTAAGCCAAGAGCAAATTGATGCCCTGCTGAAGGCGGTAAATGAAGGCGAGGAAATGCCAGCTTT
CGCACAAGAAGCAGGAAAGCAAGAGAAATTTCAAGAGTATGATTTTAATAGACCAGAGAAGTTCGGTGTTGAGCATTTAC
GTAGTTTGCAAGCGATTGCTTCTACGTTTGGAAAGCAGACGTCACAGACGTTATCAGCGCGTATGCGTATTCCGATTGAA
CTAGAACCTTCAACAGTTGAGCAAGTTCCATTTACGAGTGAGTATGTGGAGAAGATGCCGAAAGATTATTATTTATATTG
CGTAATTGATCTCGGTTTACCAGAGCTTGGAGAAATTGTTATTGAGATTGATTTAGCATTCGTTATTTATATTCATGAAT
GTTGGCTTGGTGGAGATAGTAAACGTAACTTTACGATGCGTAGACCGTTAACAGCGTTTGAGTTTTTAACGCTTGATAAT
ATATTCTTGCTTCTTTGTAAAAATTTAGAGCAATCATTTGAAAGTGTTGTTGCGATTGAACCGAAATTTGTAACGACAGA
AACAGATCCGAATGCACTAAAGATTACGACAGCGAGCGATATTATTTCATTACTTAACGTAAATATGAAAACAGATTTTT
GGAATACGACGGTGCGTATCGGAATTCCATTCTTATCTGTTGAAGAAATTATGGATAAGTTAACGTCTGAAAATATTGTC
GAACATTCTTCAGATAAACGTAAAAAGTATACGTCTGAAGTGGAAGTGAAAGTAAATCAAGTGTACAAACCTGTTCACGT
TGCAATTGGTGAGCAGAAAATGACAATGAGTGAGATTGAACAAATTGAAGAAGGCGATATTATTCCGCTTCATACGAAAG
TTTCGGATGAATTACTTGGTTATGTAGATGGAAAGCATAAATTTAATTGTTTTATTGGAAAAGATGGAACGCGTAAGGCG
CTCCTATTTAAAAGTTTTGTAGAGTAG

Upstream 100 bases:

>100_bases
TGCTTGAGAATGAAGAGATTGGAACCGGAAAGATTTTGACGAAGAATGGAAAGATGTACGTTGAAATCGTTGAATTGAAA
AGGTAGGGAAGGGGATTGTC

Downstream 100 bases:

>100_bases
GAGGATCCATATGAAGCATGAAGTATCTCCCGTGTCATTAATGGGATTAGAAGATTTTGCAGGAAAGCGAAATGAAGCAG
GTAAGGCACATATTGATACT

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSGEKLSQEQIDALLKAVNEGEEMPAFAQEAGKQEKFQEYDFNRPEKFGVEHLRSLQAIASTFGKQTSQTLSARMRIPIE
LEPSTVEQVPFTSEYVEKMPKDYYLYCVIDLGLPELGEIVIEIDLAFVIYIHECWLGGDSKRNFTMRRPLTAFEFLTLDN
IFLLLCKNLEQSFESVVAIEPKFVTTETDPNALKITTASDIISLLNVNMKTDFWNTTVRIGIPFLSVEEIMDKLTSENIV
EHSSDKRKKYTSEVEVKVNQVYKPVHVAIGEQKMTMSEIEQIEEGDIIPLHTKVSDELLGYVDGKHKFNCFIGKDGTRKA
LLFKSFVE

Sequences:

>Translated_328_residues
MSGEKLSQEQIDALLKAVNEGEEMPAFAQEAGKQEKFQEYDFNRPEKFGVEHLRSLQAIASTFGKQTSQTLSARMRIPIE
LEPSTVEQVPFTSEYVEKMPKDYYLYCVIDLGLPELGEIVIEIDLAFVIYIHECWLGGDSKRNFTMRRPLTAFEFLTLDN
IFLLLCKNLEQSFESVVAIEPKFVTTETDPNALKITTASDIISLLNVNMKTDFWNTTVRIGIPFLSVEEIMDKLTSENIV
EHSSDKRKKYTSEVEVKVNQVYKPVHVAIGEQKMTMSEIEQIEEGDIIPLHTKVSDELLGYVDGKHKFNCFIGKDGTRKA
LLFKSFVE
>Mature_327_residues
SGEKLSQEQIDALLKAVNEGEEMPAFAQEAGKQEKFQEYDFNRPEKFGVEHLRSLQAIASTFGKQTSQTLSARMRIPIEL
EPSTVEQVPFTSEYVEKMPKDYYLYCVIDLGLPELGEIVIEIDLAFVIYIHECWLGGDSKRNFTMRRPLTAFEFLTLDNI
FLLLCKNLEQSFESVVAIEPKFVTTETDPNALKITTASDIISLLNVNMKTDFWNTTVRIGIPFLSVEEIMDKLTSENIVE
HSSDKRKKYTSEVEVKVNQVYKPVHVAIGEQKMTMSEIEQIEEGDIIPLHTKVSDELLGYVDGKHKFNCFIGKDGTRKAL
LFKSFVE

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37416; Mature: 37285

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGEKLSQEQIDALLKAVNEGEEMPAFAQEAGKQEKFQEYDFNRPEKFGVEHLRSLQAIA
CCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
STFGKQTSQTLSARMRIPIELEPSTVEQVPFTSEYVEKMPKDYYLYCVIDLGLPELGEIV
HHHCCHHHHHHHHHEEEEEEECCCCHHCCCCCHHHHHHCCCCEEEEEEEECCCCCCCCEE
IEIDLAFVIYIHECWLGGDSKRNFTMRRPLTAFEFLTLDNIFLLLCKNLEQSFESVVAIE
EEEHHHHEEEEHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
PKFVTTETDPNALKITTASDIISLLNVNMKTDFWNTTVRIGIPFLSVEEIMDKLTSENIV
CEEEEECCCCCEEEEEEHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
EHSSDKRKKYTSEVEVKVNQVYKPVHVAIGEQKMTMSEIEQIEEGDIIPLHTKVSDELLG
HCCCHHHHHCCHHHEEEHHHHCCCHHEEECCCHHHHHHHHHHCCCCEEEEECCHHHHHHH
YVDGKHKFNCFIGKDGTRKALLFKSFVE
HCCCCEEEEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SGEKLSQEQIDALLKAVNEGEEMPAFAQEAGKQEKFQEYDFNRPEKFGVEHLRSLQAIA
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
STFGKQTSQTLSARMRIPIELEPSTVEQVPFTSEYVEKMPKDYYLYCVIDLGLPELGEIV
HHHCCHHHHHHHHHEEEEEEECCCCHHCCCCCHHHHHHCCCCEEEEEEEECCCCCCCCEE
IEIDLAFVIYIHECWLGGDSKRNFTMRRPLTAFEFLTLDNIFLLLCKNLEQSFESVVAIE
EEEHHHHEEEEHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
PKFVTTETDPNALKITTASDIISLLNVNMKTDFWNTTVRIGIPFLSVEEIMDKLTSENIV
CEEEEECCCCCEEEEEEHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
EHSSDKRKKYTSEVEVKVNQVYKPVHVAIGEQKMTMSEIEQIEEGDIIPLHTKVSDELLG
HCCCHHHHHCCHHHEEEHHHHCCCHHEEECCCHHHHHHHHHHCCCCEEEEECCHHHHHHH
YVDGKHKFNCFIGKDGTRKALLFKSFVE
HCCCCEEEEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8755894; 9665876; 8529894 [H]