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The map label for this gene is mrdA [H]

Identifier: 218233497

GI number: 218233497

Start: 2419301

End: 2421058

Strand: Reverse

Name: mrdA [H]

Synonym: BCB4264_A2501

Alternate gene names: 218233497

Gene position: 2421058-2419301 (Counterclockwise)

Preceding gene: 218235250

Following gene: 218231544

Centisome position: 44.68

GC content: 33.33

Gene sequence:

>1758_bases
ATGTTACATACAAGAAGATGGAGATTTTTTGCGGTTCTTTCTATTGTAGGTTTGATTTTACTAATTTTATTAAAAATCAA
CTTCTTTTCAATTACTATCGCAACTTCTTCAGCTGAAAGAGGAAAGATTTTTGATAAAAATGGTGTAATGCTAGCTACAA
ATAAGAAAGTTAAGTCTCTATATTGCACTTCTAATGATGAAAAGCTATCGAAACAAGATACATCAAACACATTAGCTTTT
TTTAACCAATTTTCAAGCGAGTTTCAACATGATATTTCTACAGAGAACATAAAATCTCAATTACAACAATCTTGTAAAAA
GAAGACTATGAATGATATACCGTTATACTCTGATATAAATGAGAATGAACTTGCATTCATAAACAAAAACAAACCCAATA
ATGTAATAATTAAAGATGAATGGATTCGATATTACCCTAAACACGAAATTGGTTCACAAGTAATTGGGTATGTAGAAAAT
GACCTTAAGTCCAAGCATATGGCTGTTGGAAAAAGCGGGATTGAGCTGCAATATGAAAATGATTTAAAAGGAAAACCAGG
CAAAACTCTTATTTTTAAATTGAATAATAAAAAGTTCTTATGGAACATTCAAAAAGTACAAAAAGGAAAAGATGTGCGGC
TAGCTTTAGACTCTGAGTTGCAGCAAAAGGCAGAAGAAGCATTAAAATCTCAAATTAAGAAAACACCTGATGCTAAAGCT
GGTTATGCTATAGTGAGCGATGCAAAAACTGGCGCAATTTTAACTCTGGCCAACTCGGTAGCTTTTAATCCAAATATATT
AAATACACATGTATCTTCTAGAAAAGAAAACATTAAATCTCTTTCGCAAAATAAAGCAATTCAAAAATTAAAGTATGGTG
AATCTTATGTAAATATGGCTTCTACTATAAAACCACTCACTATTTTAATTGGATTAAATGAAAAGCTTTTCCAACCAGAA
GATACTTATTTAGATAAAGGTACTTTTCAGTATGATAATCAAAATAACATTACGAATGCGCCTGGAACACCAACAGGGGA
GATTACACCGAGGCAGGCTATCATTAATTCATCTAACACATTTATGACAGCAAAAGTAGCTCTGCCCTTATTCAACCAAA
ATAATGGAAATATAGAAAAAGTGGCACATATATGGACAGATTATTTAATGCAATTCGGCCTACGTTCTAAAACTGGGATT
GATTTACCCTTTGAAGAAGACGGACAATATGAATTCCATCCATCTAATAAATTTGAAAATGGGATTTCAGCTTTATTAAA
TGCCTCTTGGGGAGGAAATGAAGTGCATACTCCTCTTCAACTCGCTCAATATGCAGCAACTTTGGCAAGTAAAGGCGACA
AATATAAGCCTCAAATCGTAAGTGCGATTATTGGTCAAGATGGTAAGGAAACCAAAAAGTTTAAACCCATTTTAGAAAGT
TCAAATCGTTACCCGATGAACTTTTGGAGTGTCGTGCAAGGTGGGATGAGTCAAAATATAGAGGAAATTAAAAAGTTGCC
CTTTGACGTCGCAGGTAAAACAGGTATTACAGGTTTTCCGAATGAGCAAGCAAGAATGATAAATCACTCTCTTTTCATTG
CGTATGCTCCTACGGAAGATCCACAAATTGCCGTTTCTGTCGTTATCCCTGGTAGTAATTCAGAAAAAAACATTGCCGCT
CTCGTTACATCAGAAATTTTAGAGTACTGGAATACTCTTCAAAACGAAAATAAAAATAGAGAGGAAGGTTCACTTTAA

Upstream 100 bases:

>100_bases
CTGTAATACTTAATAGACAACCTAAACAATAAAGTTGTTCCACATTTACAAAAAAAATAATTAAATATAAGATGAAATAG
GCTACATAAGGGAGGGTTAT

Downstream 100 bases:

>100_bases
TGAACCTTCCTCTTTTACCATATAGAATCTATATTTATTATTTTCCATATACCTTCATTATTCTTTTCGAACATAAATGA
ATATTTAATATCAGTTAAAA

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MLHTRRWRFFAVLSIVGLILLILLKINFFSITIATSSAERGKIFDKNGVMLATNKKVKSLYCTSNDEKLSKQDTSNTLAF
FNQFSSEFQHDISTENIKSQLQQSCKKKTMNDIPLYSDINENELAFINKNKPNNVIIKDEWIRYYPKHEIGSQVIGYVEN
DLKSKHMAVGKSGIELQYENDLKGKPGKTLIFKLNNKKFLWNIQKVQKGKDVRLALDSELQQKAEEALKSQIKKTPDAKA
GYAIVSDAKTGAILTLANSVAFNPNILNTHVSSRKENIKSLSQNKAIQKLKYGESYVNMASTIKPLTILIGLNEKLFQPE
DTYLDKGTFQYDNQNNITNAPGTPTGEITPRQAIINSSNTFMTAKVALPLFNQNNGNIEKVAHIWTDYLMQFGLRSKTGI
DLPFEEDGQYEFHPSNKFENGISALLNASWGGNEVHTPLQLAQYAATLASKGDKYKPQIVSAIIGQDGKETKKFKPILES
SNRYPMNFWSVVQGGMSQNIEEIKKLPFDVAGKTGITGFPNEQARMINHSLFIAYAPTEDPQIAVSVVIPGSNSEKNIAA
LVTSEILEYWNTLQNENKNREEGSL

Sequences:

>Translated_585_residues
MLHTRRWRFFAVLSIVGLILLILLKINFFSITIATSSAERGKIFDKNGVMLATNKKVKSLYCTSNDEKLSKQDTSNTLAF
FNQFSSEFQHDISTENIKSQLQQSCKKKTMNDIPLYSDINENELAFINKNKPNNVIIKDEWIRYYPKHEIGSQVIGYVEN
DLKSKHMAVGKSGIELQYENDLKGKPGKTLIFKLNNKKFLWNIQKVQKGKDVRLALDSELQQKAEEALKSQIKKTPDAKA
GYAIVSDAKTGAILTLANSVAFNPNILNTHVSSRKENIKSLSQNKAIQKLKYGESYVNMASTIKPLTILIGLNEKLFQPE
DTYLDKGTFQYDNQNNITNAPGTPTGEITPRQAIINSSNTFMTAKVALPLFNQNNGNIEKVAHIWTDYLMQFGLRSKTGI
DLPFEEDGQYEFHPSNKFENGISALLNASWGGNEVHTPLQLAQYAATLASKGDKYKPQIVSAIIGQDGKETKKFKPILES
SNRYPMNFWSVVQGGMSQNIEEIKKLPFDVAGKTGITGFPNEQARMINHSLFIAYAPTEDPQIAVSVVIPGSNSEKNIAA
LVTSEILEYWNTLQNENKNREEGSL
>Mature_585_residues
MLHTRRWRFFAVLSIVGLILLILLKINFFSITIATSSAERGKIFDKNGVMLATNKKVKSLYCTSNDEKLSKQDTSNTLAF
FNQFSSEFQHDISTENIKSQLQQSCKKKTMNDIPLYSDINENELAFINKNKPNNVIIKDEWIRYYPKHEIGSQVIGYVEN
DLKSKHMAVGKSGIELQYENDLKGKPGKTLIFKLNNKKFLWNIQKVQKGKDVRLALDSELQQKAEEALKSQIKKTPDAKA
GYAIVSDAKTGAILTLANSVAFNPNILNTHVSSRKENIKSLSQNKAIQKLKYGESYVNMASTIKPLTILIGLNEKLFQPE
DTYLDKGTFQYDNQNNITNAPGTPTGEITPRQAIINSSNTFMTAKVALPLFNQNNGNIEKVAHIWTDYLMQFGLRSKTGI
DLPFEEDGQYEFHPSNKFENGISALLNASWGGNEVHTPLQLAQYAATLASKGDKYKPQIVSAIIGQDGKETKKFKPILES
SNRYPMNFWSVVQGGMSQNIEEIKKLPFDVAGKTGITGFPNEQARMINHSLFIAYAPTEDPQIAVSVVIPGSNSEKNIAA
LVTSEILEYWNTLQNENKNREEGSL

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=624, Percent_Identity=21.7948717948718, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1786272, Length=599, Percent_Identity=23.873121869783, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 65614; Mature: 65614

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHTRRWRFFAVLSIVGLILLILLKINFFSITIATSSAERGKIFDKNGVMLATNKKVKSL
CCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCEECCCCEEEEECCCEEEE
YCTSNDEKLSKQDTSNTLAFFNQFSSEFQHDISTENIKSQLQQSCKKKTMNDIPLYSDIN
EECCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
ENELAFINKNKPNNVIIKDEWIRYYPKHEIGSQVIGYVENDLKSKHMAVGKSGIELQYEN
CCCEEEEECCCCCEEEEECHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
DLKGKPGKTLIFKLNNKKFLWNIQKVQKGKDVRLALDSELQQKAEEALKSQIKKTPDAKA
CCCCCCCCEEEEEECCCEEEEEHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCC
GYAIVSDAKTGAILTLANSVAFNPNILNTHVSSRKENIKSLSQNKAIQKLKYGESYVNMA
CEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
STIKPLTILIGLNEKLFQPEDTYLDKGTFQYDNQNNITNAPGTPTGEITPRQAIINSSNT
HHCCEEEEEEECCHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCC
FMTAKVALPLFNQNNGNIEKVAHIWTDYLMQFGLRSKTGIDLPFEEDGQYEFHPSNKFEN
EEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCHHH
GISALLNASWGGNEVHTPLQLAQYAATLASKGDKYKPQIVSAIIGQDGKETKKFKPILES
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHC
SNRYPMNFWSVVQGGMSQNIEEIKKLPFDVAGKTGITGFPNEQARMINHSLFIAYAPTED
CCCCCCHHHHHHHCHHHCCHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCCC
PQIAVSVVIPGSNSEKNIAALVTSEILEYWNTLQNENKNREEGSL
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLHTRRWRFFAVLSIVGLILLILLKINFFSITIATSSAERGKIFDKNGVMLATNKKVKSL
CCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCEECCCCEEEEECCCEEEE
YCTSNDEKLSKQDTSNTLAFFNQFSSEFQHDISTENIKSQLQQSCKKKTMNDIPLYSDIN
EECCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
ENELAFINKNKPNNVIIKDEWIRYYPKHEIGSQVIGYVENDLKSKHMAVGKSGIELQYEN
CCCEEEEECCCCCEEEEECHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
DLKGKPGKTLIFKLNNKKFLWNIQKVQKGKDVRLALDSELQQKAEEALKSQIKKTPDAKA
CCCCCCCCEEEEEECCCEEEEEHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCC
GYAIVSDAKTGAILTLANSVAFNPNILNTHVSSRKENIKSLSQNKAIQKLKYGESYVNMA
CEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
STIKPLTILIGLNEKLFQPEDTYLDKGTFQYDNQNNITNAPGTPTGEITPRQAIINSSNT
HHCCEEEEEEECCHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCC
FMTAKVALPLFNQNNGNIEKVAHIWTDYLMQFGLRSKTGIDLPFEEDGQYEFHPSNKFEN
EEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCHHH
GISALLNASWGGNEVHTPLQLAQYAATLASKGDKYKPQIVSAIIGQDGKETKKFKPILES
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHC
SNRYPMNFWSVVQGGMSQNIEEIKKLPFDVAGKTGITGFPNEQARMINHSLFIAYAPTED
CCCCCCHHHHHHHCHHHCCHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCCC
PQIAVSVVIPGSNSEKNIAALVTSEILEYWNTLQNENKNREEGSL
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]