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The map label for this gene is ansA1 [H]

Identifier: 218233392

GI number: 218233392

Start: 1483004

End: 1483936

Strand: Reverse

Name: ansA1 [H]

Synonym: BCB4264_A1551

Alternate gene names: 218233392

Gene position: 1483936-1483004 (Counterclockwise)

Preceding gene: 218233966

Following gene: 218234259

Centisome position: 27.38

GC content: 37.3

Gene sequence:

>933_bases
ATGGAGGAAGATAAAGAAACTGGGGTCGTACAACCAGGTGAAAAAAATCCTCTTTTAAAATTCATTCCTGATTTAGAAGG
CGATGTTGATTTAATCGTTGAAGATGTGTTCCACCTACCATCTCCTCATATGACACCGAGCGAAATGTTACAATTACAAG
TCATCATTGATGAAAGAGTAAAACAAGACAACATTCATGGCGTAGTCATTACGCATGGCACGGATACATTAGAAGAAACA
GCTTATTTCTTAGATTTAACAGTACAAGCAACTATTCCGATCGTAGTGACAGGTGCAATGCGCTCTAGCAATGAGTTAGG
TGCTGATGGTCTATATAACTTCTTATCAGCTGTAAAAGTTGCTAGTTGTGATGAAGCTGCAGAAAAAGGTGTTTTAGTCG
TATTAAACGACGAAATTCATTGCGCTACAAATGTAACGAAAACACATACAAGTAATGTTGCAACATTCCAAAGCCCACAA
TACGGACCAATTGGAATGGTAACAAAACGCGGCGTTGTCTTCCATCACGCTTTAGTACATCACGAAACATATACGATAAA
CAAAGTGTCTAAAAATGTAGTTGTTTTAAAAGCATATGCTGGCATGGATGATACATTATTAGCAGCAATCGAAAATCTTC
CTGTAGACGGCATTGTTATCGAAGCTCTTGGACAAGGTAATCTCCCTCCAAGAACACTACCTAGTCTAGAAAGATTATTA
AATAGAGGTATTCCTGTCGTATTAGTTTCTCGCTGTTTCAACGGTATCGTTCAAGACGTATATTCGTATGAAGGTGGCGG
TAAACACTTAAAAGATATGGGCATTGTATTCACATACGGCTTAAATGCACAAAAAGCACGCATTAAATTACTTGTTGCAT
TAGAGAATACAAATTCTCATGAATCCATTCAAAATATGTTTATGCACGAATAA

Upstream 100 bases:

>100_bases
TTTACTCAAAACATGTTATGTTCTAAATGTATTTTTTTACTTGAAAGGGGTATAAGCATTGAAAAGAATCCTAGTTTTAC
ACACAGGTGGAACAATTGCA

Downstream 100 bases:

>100_bases
AAAGGAGCTGTCGAGGTTTTCCCGACAGCTCCTTTTTATTTTTCTCTTGATGCAATGGTTTGCGCAATCGCATCTCCATG
AAATCTCCCGTTCTCGATAA

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERVKQDNIHGVVITHGTDTLEET
AYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKVASCDEAAEKGVLVVLNDEIHCATNVTKTHTSNVATFQSPQ
YGPIGMVTKRGVVFHHALVHHETYTINKVSKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQGNLPPRTLPSLERLL
NRGIPVVLVSRCFNGIVQDVYSYEGGGKHLKDMGIVFTYGLNAQKARIKLLVALENTNSHESIQNMFMHE

Sequences:

>Translated_310_residues
MEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERVKQDNIHGVVITHGTDTLEET
AYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKVASCDEAAEKGVLVVLNDEIHCATNVTKTHTSNVATFQSPQ
YGPIGMVTKRGVVFHHALVHHETYTINKVSKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQGNLPPRTLPSLERLL
NRGIPVVLVSRCFNGIVQDVYSYEGGGKHLKDMGIVFTYGLNAQKARIKLLVALENTNSHESIQNMFMHE
>Mature_310_residues
MEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERVKQDNIHGVVITHGTDTLEET
AYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKVASCDEAAEKGVLVVLNDEIHCATNVTKTHTSNVATFQSPQ
YGPIGMVTKRGVVFHHALVHHETYTINKVSKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQGNLPPRTLPSLERLL
NRGIPVVLVSRCFNGIVQDVYSYEGGGKHLKDMGIVFTYGLNAQKARIKLLVALENTNSHESIQNMFMHE

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=235, Percent_Identity=28.936170212766, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1789327, Length=297, Percent_Identity=36.7003367003367, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1788065, Length=223, Percent_Identity=27.8026905829596, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17558278, Length=251, Percent_Identity=25.0996015936255, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193207372, Length=252, Percent_Identity=25, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17558280, Length=251, Percent_Identity=25.0996015936255, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193207374, Length=236, Percent_Identity=26.271186440678, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323189, Length=304, Percent_Identity=29.2763157894737, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323187, Length=304, Percent_Identity=29.2763157894737, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323186, Length=304, Percent_Identity=29.2763157894737, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323184, Length=304, Percent_Identity=29.2763157894737, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320527, Length=299, Percent_Identity=29.0969899665552, Blast_Score=109, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24645530, Length=238, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24639609, Length=261, Percent_Identity=23.7547892720307, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 33944; Mature: 33944

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERV
CCCCCCCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHCCCCCCCHHHHEEEEEEEHHHC
KQDNIHGVVITHGTDTLEETAYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKV
CCCCCCEEEEECCCCCHHHHEEEEEEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
ASCDEAAEKGVLVVLNDEIHCATNVTKTHTSNVATFQSPQYGPIGMVTKRGVVFHHALVH
HCCHHHHHCCEEEEECCCEEEECCCCCCCCCCEEEECCCCCCCCCEEECCCHHHHHHHHH
HETYTINKVSKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQGNLPPRTLPSLERLL
CCEEEEEECCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHH
NRGIPVVLVSRCFNGIVQDVYSYEGGGKHLKDMGIVFTYGLNAQKARIKLLVALENTNSH
HCCCCEEHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCCCCEEEEEEEEEECCCCH
ESIQNMFMHE
HHHHHHHCCC
>Mature Secondary Structure
MEEDKETGVVQPGEKNPLLKFIPDLEGDVDLIVEDVFHLPSPHMTPSEMLQLQVIIDERV
CCCCCCCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHCCCCCCCHHHHEEEEEEEHHHC
KQDNIHGVVITHGTDTLEETAYFLDLTVQATIPIVVTGAMRSSNELGADGLYNFLSAVKV
CCCCCCEEEEECCCCCHHHHEEEEEEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
ASCDEAAEKGVLVVLNDEIHCATNVTKTHTSNVATFQSPQYGPIGMVTKRGVVFHHALVH
HCCHHHHHCCEEEEECCCEEEECCCCCCCCCCEEEECCCCCCCCCEEECCCHHHHHHHHH
HETYTINKVSKNVVVLKAYAGMDDTLLAAIENLPVDGIVIEALGQGNLPPRTLPSLERLL
CCEEEEEECCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHH
NRGIPVVLVSRCFNGIVQDVYSYEGGGKHLKDMGIVFTYGLNAQKARIKLLVALENTNSH
HCCCCEEHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCCCCEEEEEEEEEECCCCH
ESIQNMFMHE
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]