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The map label for this gene is amaA [H]

Identifier: 218233174

GI number: 218233174

Start: 733375

End: 734550

Strand: Direct

Name: amaA [H]

Synonym: BCB4264_A0740

Alternate gene names: 218233174

Gene position: 733375-734550 (Clockwise)

Preceding gene: 218231362

Following gene: 218232600

Centisome position: 13.53

GC content: 39.46

Gene sequence:

>1176_bases
ATGGATGTTGCAAAGGAACTTGTTTTGTCAAAAAATCAGTTAGTTGAGTGGAGAAGGCATTTTCATAAGTATCCAGAGTT
ATCTTTTCAAGAAGAGAAAACATCGCAGTTTGTGTTCGATATACTTCGGAAATTCCCATATTTAGAAGTGTCACGGCCGA
CTAAATATAGTGTGATGGCAAAGTTAGTAGGTAAACATCCGGGCAAAACAGTTGCGATTCGAGCCGATATGGATGCTCTT
CCTATTCATGAAGAAAATGAGTTTGATTTTATTTCTACATATAAAGGTGTAATGCATGCATGTGGTCATGATGGACATAT
AGCGATATTACTTGGGGTTGTACATAAGTTGGTAGAAGAAAGAGAGAAGATTAAAGGGGAGATTCGTTTTCTATTCCAAC
ATGCTGAAGAGAATTTTCCTGGTGGTGCAGAGGAGATGGTTGCTGCAGGTGTAATGGAAGGTGTGGATTATATTATTGGT
GCTCACCTTTGGGCGTCGTTAGAGGTTGGGAAAATAGGTGTTATTTATGGTCCTGCAATGGCTGCACCGGATGTTTTTAA
AATTAAGATAGAGGGAAAAGGTGGACATGCTGGAATCCCTCATGAAACGGTGGATAGTATTGCAATCGGAACACAAGTCG
TTTCGCAACTTCAGCAAATTGTATCTCGCCTCACGAATCCATTAGATTCTCTCGTAATATCTGTTACGCAATTTCATGCG
GGGACAACTCATAATGTAATACCAGAGCAAGCCGAGATTGAAGGGACAGTAAGAAGTTTAAGACATGAATTACGAGGTGA
AACAGAGAAGAAGATTGAGCGGATTGTGAAGCATATTACGGAATCTTACGGAGCGAAATATACATTTTCATATGAATATG
GATATCGACCAGTTGTAAATGATTATGAAGCTACAGAGCTTATTGAACAAACGGCATTACAACTTTATGGAAGGGATAGA
GTTGTTCGTTTACAGCCGACGATGGCTGGAGAAGATTTTTCAGCGTTTTTACAAAAGGCACCGGGGACATTCTTTTTTAT
CGGAGCGGGAAATAAAGAGAAAGGTATTATATATCCTCACCATCACCCTCGTTTTACGATTGACGAAGATGCATTACCGA
TTGGAGTGGAAGTCTTTGTATCAGCCATTATGAACTTTATAAGTAAAGGAGAATGA

Upstream 100 bases:

>100_bases
CTTTTTGGTTTATTAACACTGAAAATTCAGAAGATTCTTTTAAAAGTTAACGATTCACGTTATAATAACATTTAACTTGG
TGAAGAAGGGGAGAAGAGAA

Downstream 100 bases:

>100_bases
GATGAAGAAAATACACATTCTAGCGCTTATCCCAGTTCTTTGTTTAGTAGTTGGACCAATATTTGCGAATTCAGTTACTC
CTTACGTATTAGGGATGCCA

Product: N-acyl-L-amino acid amidohydrolase

Products: NA

Alternate protein names: L-aminoacylase [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MDVAKELVLSKNQLVEWRRHFHKYPELSFQEEKTSQFVFDILRKFPYLEVSRPTKYSVMAKLVGKHPGKTVAIRADMDAL
PIHEENEFDFISTYKGVMHACGHDGHIAILLGVVHKLVEEREKIKGEIRFLFQHAEENFPGGAEEMVAAGVMEGVDYIIG
AHLWASLEVGKIGVIYGPAMAAPDVFKIKIEGKGGHAGIPHETVDSIAIGTQVVSQLQQIVSRLTNPLDSLVISVTQFHA
GTTHNVIPEQAEIEGTVRSLRHELRGETEKKIERIVKHITESYGAKYTFSYEYGYRPVVNDYEATELIEQTALQLYGRDR
VVRLQPTMAGEDFSAFLQKAPGTFFFIGAGNKEKGIIYPHHHPRFTIDEDALPIGVEVFVSAIMNFISKGE

Sequences:

>Translated_391_residues
MDVAKELVLSKNQLVEWRRHFHKYPELSFQEEKTSQFVFDILRKFPYLEVSRPTKYSVMAKLVGKHPGKTVAIRADMDAL
PIHEENEFDFISTYKGVMHACGHDGHIAILLGVVHKLVEEREKIKGEIRFLFQHAEENFPGGAEEMVAAGVMEGVDYIIG
AHLWASLEVGKIGVIYGPAMAAPDVFKIKIEGKGGHAGIPHETVDSIAIGTQVVSQLQQIVSRLTNPLDSLVISVTQFHA
GTTHNVIPEQAEIEGTVRSLRHELRGETEKKIERIVKHITESYGAKYTFSYEYGYRPVVNDYEATELIEQTALQLYGRDR
VVRLQPTMAGEDFSAFLQKAPGTFFFIGAGNKEKGIIYPHHHPRFTIDEDALPIGVEVFVSAIMNFISKGE
>Mature_391_residues
MDVAKELVLSKNQLVEWRRHFHKYPELSFQEEKTSQFVFDILRKFPYLEVSRPTKYSVMAKLVGKHPGKTVAIRADMDAL
PIHEENEFDFISTYKGVMHACGHDGHIAILLGVVHKLVEEREKIKGEIRFLFQHAEENFPGGAEEMVAAGVMEGVDYIIG
AHLWASLEVGKIGVIYGPAMAAPDVFKIKIEGKGGHAGIPHETVDSIAIGTQVVSQLQQIVSRLTNPLDSLVISVTQFHA
GTTHNVIPEQAEIEGTVRSLRHELRGETEKKIERIVKHITESYGAKYTFSYEYGYRPVVNDYEATELIEQTALQLYGRDR
VVRLQPTMAGEDFSAFLQKAPGTFFFIGAGNKEKGIIYPHHHPRFTIDEDALPIGVEVFVSAIMNFISKGE

Specific function: Hydrolyzes most efficiently N-acetyl derivatives of aromatic amino acids but is also active on other amino acids. L- stereospecific [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=424, Percent_Identity=25.2358490566038, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.14 [H]

Molecular weight: Translated: 43872; Mature: 43872

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVAKELVLSKNQLVEWRRHFHKYPELSFQEEKTSQFVFDILRKFPYLEVSRPTKYSVMA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCHHHHHH
KLVGKHPGKTVAIRADMDALPIHEENEFDFISTYKGVMHACGHDGHIAILLGVVHKLVEE
HHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
REKIKGEIRFLFQHAEENFPGGAEEMVAAGVMEGVDYIIGAHLWASLEVGKIGVIYGPAM
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCC
AAPDVFKIKIEGKGGHAGIPHETVDSIAIGTQVVSQLQQIVSRLTNPLDSLVISVTQFHA
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC
GTTHNVIPEQAEIEGTVRSLRHELRGETEKKIERIVKHITESYGAKYTFSYEYGYRPVVN
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
DYEATELIEQTALQLYGRDRVVRLQPTMAGEDFSAFLQKAPGTFFFIGAGNKEKGIIYPH
CHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCCCEECCC
HHPRFTIDEDALPIGVEVFVSAIMNFISKGE
CCCCEEECCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDVAKELVLSKNQLVEWRRHFHKYPELSFQEEKTSQFVFDILRKFPYLEVSRPTKYSVMA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCHHHHHH
KLVGKHPGKTVAIRADMDALPIHEENEFDFISTYKGVMHACGHDGHIAILLGVVHKLVEE
HHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
REKIKGEIRFLFQHAEENFPGGAEEMVAAGVMEGVDYIIGAHLWASLEVGKIGVIYGPAM
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCC
AAPDVFKIKIEGKGGHAGIPHETVDSIAIGTQVVSQLQQIVSRLTNPLDSLVISVTQFHA
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC
GTTHNVIPEQAEIEGTVRSLRHELRGETEKKIERIVKHITESYGAKYTFSYEYGYRPVVN
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
DYEATELIEQTALQLYGRDRVVRLQPTMAGEDFSAFLQKAPGTFFFIGAGNKEKGIIYPH
CHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCCCEECCC
HHPRFTIDEDALPIGVEVFVSAIMNFISKGE
CCCCEEECCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8285691; 9023955 [H]