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The map label for this gene is rihA [H]

Identifier: 218232861

GI number: 218232861

Start: 3574378

End: 3575310

Strand: Reverse

Name: rihA [H]

Synonym: BCB4264_A3659

Alternate gene names: 218232861

Gene position: 3575310-3574378 (Counterclockwise)

Preceding gene: 218233481

Following gene: 218234272

Centisome position: 65.98

GC content: 35.26

Gene sequence:

>933_bases
ATGAAAAAAGTGTTATTTTTAGGAGACCCAGGAATTGATGACTCTTTAGCAATTATGTATGGATTGTTGCATCCTGATAT
TGATATTGTTGGTGTAGTAACGGGATATGGAAATGTAACACAAGAAAAGGCGACAAGTAATGCCGCATATTTATTGCAAA
TGGCAGGACGGGAAGATATACCTGTTATTAATGGTGCGAAAATTCCTTTATCCGGAGATATTACGACGTATTATCCAGAA
ATTCATGGGACAGACGGTTTAGGGCCAATTAAACCTCCAAAAACTTTTTCACCAAATATAAAGCCTTTTTGTACATTTTT
TGATCTTCTTGAAAAATATAAAGGAGAATTAATAATAGTCGATGCTGGTAGATCAACAACATTAGCGACAGCATTTATTT
TAGAAAAGCCGATGATGAAGTATGTGAAAGAATATTATATAATGGGCGGTGCTTTCTTAATGCCTGGCAATGTAACACCA
GTCGCAGAGGCGAATTTTCATGGTGATCCCATTGCGTCACAATTAGTTATGCAAAATGCCAAAAATGTGACATTAGTGCC
ACTTAACGTTACATCTGAAGCAATTATTACACCGGAAATGGTAAAATATATTACGAAACACTCTAAAACGAGTTTTAATA
AGTTAATCGAGCCGATATTTACGTATTATTATAAAGCATATAAAAAGTTAAACCCGAAAATAACAGGTAGTCCAGTACAT
GACGTCGTTACAATGATGGTTGCGGCAAATCCATCCATTTTAGATTATGTATATCGTCGTGTCGATGTAGATACAGTTGG
AATTGCAAAAGGAGAGAGTATTGCTGATTTTCGGCCTCAACCTGATGCAAAAGCATTAAAAAATTGGGTGCGAATTGGTT
GGTCGTTACATTATAAAAAGTTTCTTGAGGATTTTGTGAAATTTATGACGTAG

Upstream 100 bases:

>100_bases
ATAAGACTGCGTTTTTTCTTATGAATTTGTTCTTTTTTTATTCTGTATATCCACGGTTATTCATAAATTAATAACAACTG
AGGGGATGGAGTTGAGGTCG

Downstream 100 bases:

>100_bases
ACATAATAGGAGCATACAAGTTAAAATAATGGAGATGGTTATTTAAATGACCATCTCCATTATTTTGTTTTTAAATGATA
AATAGAGAAGAGTTACACAA

Product: inosine-uridine preferring nucleoside hydrolase family protein

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQMAGREDIPVINGAKIPLSGDITTYYPE
IHGTDGLGPIKPPKTFSPNIKPFCTFFDLLEKYKGELIIVDAGRSTTLATAFILEKPMMKYVKEYYIMGGAFLMPGNVTP
VAEANFHGDPIASQLVMQNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYKKLNPKITGSPVH
DVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLHYKKFLEDFVKFMT

Sequences:

>Translated_310_residues
MKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQMAGREDIPVINGAKIPLSGDITTYYPE
IHGTDGLGPIKPPKTFSPNIKPFCTFFDLLEKYKGELIIVDAGRSTTLATAFILEKPMMKYVKEYYIMGGAFLMPGNVTP
VAEANFHGDPIASQLVMQNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYKKLNPKITGSPVH
DVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLHYKKFLEDFVKFMT
>Mature_310_residues
MKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQMAGREDIPVINGAKIPLSGDITTYYPE
IHGTDGLGPIKPPKTFSPNIKPFCTFFDLLEKYKGELIIVDAGRSTTLATAFILEKPMMKYVKEYYIMGGAFLMPGNVTP
VAEANFHGDPIASQLVMQNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYKKLNPKITGSPVH
DVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLHYKKFLEDFVKFMT

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=315, Percent_Identity=29.8412698412698, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1788486, Length=279, Percent_Identity=27.9569892473118, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1786213, Length=310, Percent_Identity=28.3870967741935, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32565474, Length=196, Percent_Identity=30.1020408163265, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17565698, Length=200, Percent_Identity=29.5, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320608, Length=277, Percent_Identity=31.7689530685921, Blast_Score=119, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 34446; Mature: 34446

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQMAGREDI
CCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCHHHHEEECCCCCC
PVINGAKIPLSGDITTYYPEIHGTDGLGPIKPPKTFSPNIKPFCTFFDLLEKYKGELIIV
CEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEE
DAGRSTTLATAFILEKPMMKYVKEYYIMGGAFLMPGNVTPVAEANFHGDPIASQLVMQNA
ECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCC
KNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYKKLNPKITGSPVH
CCEEEEEECCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
DVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLHYKK
HHHHHHHHCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FLEDFVKFMT
HHHHHHHHHC
>Mature Secondary Structure
MKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQMAGREDI
CCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCHHHHEEECCCCCC
PVINGAKIPLSGDITTYYPEIHGTDGLGPIKPPKTFSPNIKPFCTFFDLLEKYKGELIIV
CEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEE
DAGRSTTLATAFILEKPMMKYVKEYYIMGGAFLMPGNVTPVAEANFHGDPIASQLVMQNA
ECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCC
KNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYKKLNPKITGSPVH
CCEEEEEECCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
DVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLHYKK
HHHHHHHHCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FLEDFVKFMT
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA