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The map label for this gene is amhX [H]

Identifier: 218232644

GI number: 218232644

Start: 365596

End: 366711

Strand: Direct

Name: amhX [H]

Synonym: BCB4264_A0389

Alternate gene names: 218232644

Gene position: 365596-366711 (Clockwise)

Preceding gene: 218231806

Following gene: 218233419

Centisome position: 6.75

GC content: 39.43

Gene sequence:

>1116_bases
TTGAAAACATTAGAACTACAAGAGCGCTTAACGGAGATTTTTCAGCATTTACACGAAAACCCTGAAGTAAGTTGGAAAGA
ATATGAAACGACAGCGTATATTACTGACTTTTTAAAAGAAGAAGGAATTTCATATAGAACATTTGATGATTGTCCAGGTG
TAATTGCTGAGATTGGAGGCGGGAATCCAGTTATTGCGATTCGTGCTGATATGGATGCACTTTGGCAAGAAGTAAATGGA
GAATTTAAAGCGAACCATTCATGTGGCCATGATGCTCATATGACCATTGTAATGGGACTTATTTTACAATTGAAAAATAT
GAGATGGAAAAGTGGTACGGTTAGATTTATTTTTCAGCCAGCAGAAGAGAAGGGGAATGGTGCTTTGAAGATGGTGGAGA
AAGGTGCTGTAGATGATGCTGATTTCTTATTTGGCGTCCATCTAAGACCAATTGAAGAACTATCTTTGAAACAAGCAGCC
CCATCTATTCGTCATGGAGCAGCAGGATTTTTAGAGGGTATGATTCATGGGGAGGATGCACACGGGGCAAGACCACATCA
AGGTGTGAATGCAATTGATGTCATTTCCATGATTAATATCGGTTTGAAAAATATCTGGCTGCCACCGCAAAGTTCTTATT
CAGTAAAAATGACGAGATGCCAAGCTGGTGGTGATAATTTAAATATTATTCCGGGAAATGGTCATTTCAGTTTAGATGTA
AGAGCAGAGAGTAATATACTATTAGAAGAATTAAAGAAAAAGATTGAGCATGTAATAGAATCAGCTGCATCGATGGGATC
AAAAACTTCATACGAATGGATGGATCTTGCACCAGGAGCAGAGGTTTCCGAAGAAGCAGAGCGCTTTATGCGTAAAGGTA
TTCTCGAAGTGTATGGGGAAGAGAGATGCACAGGGCCTCTCTATACGACAGGAAGCGATGATTTCCATTACTATACAGTG
AAAAGACCACATTTAAAAGCTGTCATGCTTGGACTAGGAGCAGACCTACAACCTGGATTACACCATCCGTATATGAGGTT
TGATCATAGTTGTATTATGGATGGGGTAGAAATATTGAAACAAACTGTATTGAAAGTGTTAGAAGACAGGGGCTAG

Upstream 100 bases:

>100_bases
TTGACTTTTTTCTTAAAATTCAATATGTTCATAAAAACATCTGTTTTTCATAAGTATAATTAACCCGAATCGTTTGTAAT
GTGGGAAGGAGACTGGAACG

Downstream 100 bases:

>100_bases
AGAGCTCCTGTTTTTTTGTCGTGAATTGTCGGCAAATCGATATATTTGAAAAAACGTTGATATATTTTGAGTTATGATAG
ATATATTGAGAAAATCGTTG

Product: amidohydrolase amhX

Products: NA

Alternate protein names: Aminoacylase [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKTLELQERLTEIFQHLHENPEVSWKEYETTAYITDFLKEEGISYRTFDDCPGVIAEIGGGNPVIAIRADMDALWQEVNG
EFKANHSCGHDAHMTIVMGLILQLKNMRWKSGTVRFIFQPAEEKGNGALKMVEKGAVDDADFLFGVHLRPIEELSLKQAA
PSIRHGAAGFLEGMIHGEDAHGARPHQGVNAIDVISMINIGLKNIWLPPQSSYSVKMTRCQAGGDNLNIIPGNGHFSLDV
RAESNILLEELKKKIEHVIESAASMGSKTSYEWMDLAPGAEVSEEAERFMRKGILEVYGEERCTGPLYTTGSDDFHYYTV
KRPHLKAVMLGLGADLQPGLHHPYMRFDHSCIMDGVEILKQTVLKVLEDRG

Sequences:

>Translated_371_residues
MKTLELQERLTEIFQHLHENPEVSWKEYETTAYITDFLKEEGISYRTFDDCPGVIAEIGGGNPVIAIRADMDALWQEVNG
EFKANHSCGHDAHMTIVMGLILQLKNMRWKSGTVRFIFQPAEEKGNGALKMVEKGAVDDADFLFGVHLRPIEELSLKQAA
PSIRHGAAGFLEGMIHGEDAHGARPHQGVNAIDVISMINIGLKNIWLPPQSSYSVKMTRCQAGGDNLNIIPGNGHFSLDV
RAESNILLEELKKKIEHVIESAASMGSKTSYEWMDLAPGAEVSEEAERFMRKGILEVYGEERCTGPLYTTGSDDFHYYTV
KRPHLKAVMLGLGADLQPGLHHPYMRFDHSCIMDGVEILKQTVLKVLEDRG
>Mature_371_residues
MKTLELQERLTEIFQHLHENPEVSWKEYETTAYITDFLKEEGISYRTFDDCPGVIAEIGGGNPVIAIRADMDALWQEVNG
EFKANHSCGHDAHMTIVMGLILQLKNMRWKSGTVRFIFQPAEEKGNGALKMVEKGAVDDADFLFGVHLRPIEELSLKQAA
PSIRHGAAGFLEGMIHGEDAHGARPHQGVNAIDVISMINIGLKNIWLPPQSSYSVKMTRCQAGGDNLNIIPGNGHFSLDV
RAESNILLEELKKKIEHVIESAASMGSKTSYEWMDLAPGAEVSEEAERFMRKGILEVYGEERCTGPLYTTGSDDFHYYTV
KRPHLKAVMLGLGADLQPGLHHPYMRFDHSCIMDGVEILKQTVLKVLEDRG

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=349, Percent_Identity=28.3667621776504, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787598, Length=281, Percent_Identity=24.9110320284698, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: NA

Molecular weight: Translated: 41387; Mature: 41387

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLELQERLTEIFQHLHENPEVSWKEYETTAYITDFLKEEGISYRTFDDCPGVIAEIGG
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCC
GNPVIAIRADMDALWQEVNGEFKANHSCGHDAHMTIVMGLILQLKNMRWKSGTVRFIFQP
CCEEEEEECCHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
AEEKGNGALKMVEKGAVDDADFLFGVHLRPIEELSLKQAAPSIRHGAAGFLEGMIHGEDA
CCCCCCCEEEEHHCCCCCCCHHEEEECCCCHHHHHHHHHCCCHHCCHHHHHHHHCCCCCC
HGARPHQGVNAIDVISMINIGLKNIWLPPQSSYSVKMTRCQAGGDNLNIIPGNGHFSLDV
CCCCCCCCCCHHHHHHHHHHCHHHEECCCCCCCEEEEEEEECCCCEEEEEECCCEEEEEE
RAESNILLEELKKKIEHVIESAASMGSKTSYEWMDLAPGAEVSEEAERFMRKGILEVYGE
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCC
ERCTGPLYTTGSDDFHYYTVKRPHLKAVMLGLGADLQPGLHHPYMRFDHSCIMDGVEILK
CCCCCCEEECCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCHHHHCHHHHHHHHHHHH
QTVLKVLEDRG
HHHHHHHHCCC
>Mature Secondary Structure
MKTLELQERLTEIFQHLHENPEVSWKEYETTAYITDFLKEEGISYRTFDDCPGVIAEIGG
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCC
GNPVIAIRADMDALWQEVNGEFKANHSCGHDAHMTIVMGLILQLKNMRWKSGTVRFIFQP
CCEEEEEECCHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
AEEKGNGALKMVEKGAVDDADFLFGVHLRPIEELSLKQAAPSIRHGAAGFLEGMIHGEDA
CCCCCCCEEEEHHCCCCCCCHHEEEECCCCHHHHHHHHHCCCHHCCHHHHHHHHCCCCCC
HGARPHQGVNAIDVISMINIGLKNIWLPPQSSYSVKMTRCQAGGDNLNIIPGNGHFSLDV
CCCCCCCCCCHHHHHHHHHHCHHHEECCCCCCCEEEEEEEECCCCEEEEEECCCEEEEEE
RAESNILLEELKKKIEHVIESAASMGSKTSYEWMDLAPGAEVSEEAERFMRKGILEVYGE
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCC
ERCTGPLYTTGSDDFHYYTVKRPHLKAVMLGLGADLQPGLHHPYMRFDHSCIMDGVEILK
CCCCCCEEECCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCHHHHCHHHHHHHHHHHH
QTVLKVLEDRG
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8768514; 8969502; 9384377; 7622480 [H]