Click here to switch to the map view.

The map label for this gene is yiaE [C]

Identifier: 218232588

GI number: 218232588

Start: 1407517

End: 1408488

Strand: Direct

Name: yiaE [C]

Synonym: BCB4264_A1468

Alternate gene names: 218232588

Gene position: 1407517-1408488 (Clockwise)

Preceding gene: 218234312

Following gene: 218232891

Centisome position: 25.97

GC content: 36.83

Gene sequence:

>972_bases
GTGGCAAAAATATTAGTAGCGGGAAAAATTCCGGAAATTGGATTAGAACTATTAAAAGATCATAATGTAGAAATGTACAA
TAAAGAGGAGTTAATTTCTATAGATGAATTAACAGAGCGTGTAAAAGATAAAGATGCATTGTTAAGTTTACTTTCGACAA
AGGTGCCAAAAGAAGTTATTGATGCAGCGCCTTATTTAAAGATTGTTGCAAACTATGGTGCTGGTTACGACAATATTGAT
TACACGTATGCGGGCGAAAAAGGAATTGCAGTTACGAATACACCGAAAGTATCAACGGAAGCGACTGCAGAATTAACATT
TGCACTTCTTTTAGCAGCGGCGCGTAGAATTCCTGAAGGGGATACGTTATGTCGTACGACTGGATTTAACGGATGGGCAC
CGTTATTTTTCTTAGGACGAGAAGTTCATGGTAAGACAATCGGAATTATAGGTCTTGGAGAAATTGGGAAGGCTGTTGCA
AAACGTGCGAAAGCATTTGGAATGGATGTTTTATATACAGGGCCAAATCGTAAGCTTGAAGCTGAAAGTGAACTGGGAGC
AACGTATGTGACGTTGGAAGAATTATTACAAACAGCAGACTTTATTACAATTAACTGTGCTTATAATCCAAAATTACATC
ATATGATTGATGAAGAGCAGTTTAAAATGATGAAGAAAACAGCGTATATTGTAAATGCTTCACGTGGACCAATTATGAAT
GAAGCAGCACTTGCTCATGCATTAAAAACAAATGAAATTGAAGGAGCAGCTCTTGACGTGTTTGAATTTGAACCGAAAAT
TACAGAAGAGCTAAAAGAATTAAAGAATGTAGTACTTGCTCCACATGTAGGGAATGCAACATTTGAAACTCGTGATGCGA
TGGCTGAAATGGCTGTCAGAAATATTTTAGCTGTATTAAACGGCGAAGAGCCAGTAACACCTGTAAATCAAAAGGTATTA
GTTACAAAATAA

Upstream 100 bases:

>100_bases
CGATTTTCAAAACTATAATCAAATTCCTTGGTTTTTATATGTGGATTTCTTACAATGAAATATAAGAGATGTCTTATTTT
CTATAGAAGAGGTGTTAGTA

Downstream 100 bases:

>100_bases
AAAGAAACCTTCCGGACTTGGAAGGTTTTCTTATTTTTCTTTATTCGCTTTTTTAGACGGTCTTTGTCTAATGAATTGAG
ATAACATGTACAAAATATAT

Product: D-isomer specific 2-hydroxyacid dehydrogenase family protein

Products: glycerate; NADP; NADPH; glyoxylate; 2-Dehydro-D-gluconate; 5-ketogluconate; L-idonate [C]

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MAKILVAGKIPEIGLELLKDHNVEMYNKEELISIDELTERVKDKDALLSLLSTKVPKEVIDAAPYLKIVANYGAGYDNID
YTYAGEKGIAVTNTPKVSTEATAELTFALLLAAARRIPEGDTLCRTTGFNGWAPLFFLGREVHGKTIGIIGLGEIGKAVA
KRAKAFGMDVLYTGPNRKLEAESELGATYVTLEELLQTADFITINCAYNPKLHHMIDEEQFKMMKKTAYIVNASRGPIMN
EAALAHALKTNEIEGAALDVFEFEPKITEELKELKNVVLAPHVGNATFETRDAMAEMAVRNILAVLNGEEPVTPVNQKVL
VTK

Sequences:

>Translated_323_residues
MAKILVAGKIPEIGLELLKDHNVEMYNKEELISIDELTERVKDKDALLSLLSTKVPKEVIDAAPYLKIVANYGAGYDNID
YTYAGEKGIAVTNTPKVSTEATAELTFALLLAAARRIPEGDTLCRTTGFNGWAPLFFLGREVHGKTIGIIGLGEIGKAVA
KRAKAFGMDVLYTGPNRKLEAESELGATYVTLEELLQTADFITINCAYNPKLHHMIDEEQFKMMKKTAYIVNASRGPIMN
EAALAHALKTNEIEGAALDVFEFEPKITEELKELKNVVLAPHVGNATFETRDAMAEMAVRNILAVLNGEEPVTPVNQKVL
VTK
>Mature_322_residues
AKILVAGKIPEIGLELLKDHNVEMYNKEELISIDELTERVKDKDALLSLLSTKVPKEVIDAAPYLKIVANYGAGYDNIDY
TYAGEKGIAVTNTPKVSTEATAELTFALLLAAARRIPEGDTLCRTTGFNGWAPLFFLGREVHGKTIGIIGLGEIGKAVAK
RAKAFGMDVLYTGPNRKLEAESELGATYVTLEELLQTADFITINCAYNPKLHHMIDEEQFKMMKKTAYIVNASRGPIMNE
AALAHALKTNEIEGAALDVFEFEPKITEELKELKNVVLAPHVGNATFETRDAMAEMAVRNILAVLNGEEPVTPVNQKVLV
TK

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=315, Percent_Identity=38.4126984126984, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI23308577, Length=322, Percent_Identity=33.2298136645963, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI61743967, Length=319, Percent_Identity=34.7962382445141, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI4557497, Length=319, Percent_Identity=34.7962382445141, Blast_Score=161, Evalue=6e-40,
Organism=Homo sapiens, GI145580578, Length=333, Percent_Identity=32.7327327327327, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI4557499, Length=333, Percent_Identity=32.7327327327327, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI145580575, Length=332, Percent_Identity=32.8313253012048, Blast_Score=155, Evalue=6e-38,
Organism=Escherichia coli, GI87082289, Length=263, Percent_Identity=38.4030418250951, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1789279, Length=265, Percent_Identity=32.8301886792453, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787645, Length=258, Percent_Identity=31.0077519379845, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1788660, Length=257, Percent_Identity=28.4046692607004, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=317, Percent_Identity=33.7539432176656, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25147481, Length=316, Percent_Identity=30.0632911392405, Blast_Score=124, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6324055, Length=235, Percent_Identity=37.4468085106383, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322116, Length=313, Percent_Identity=31.3099041533546, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320925, Length=316, Percent_Identity=31.6455696202532, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321253, Length=172, Percent_Identity=29.0697674418605, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324964, Length=275, Percent_Identity=26.1818181818182, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571528, Length=306, Percent_Identity=39.5424836601307, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24585514, Length=318, Percent_Identity=35.8490566037736, Blast_Score=168, Evalue=4e-42,
Organism=Drosophila melanogaster, GI28574282, Length=318, Percent_Identity=35.8490566037736, Blast_Score=168, Evalue=4e-42,
Organism=Drosophila melanogaster, GI45552429, Length=318, Percent_Identity=35.8490566037736, Blast_Score=168, Evalue=4e-42,
Organism=Drosophila melanogaster, GI45551003, Length=318, Percent_Identity=35.8490566037736, Blast_Score=168, Evalue=4e-42,
Organism=Drosophila melanogaster, GI28574284, Length=318, Percent_Identity=35.8490566037736, Blast_Score=168, Evalue=4e-42,
Organism=Drosophila melanogaster, GI28574286, Length=319, Percent_Identity=36.0501567398119, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI19921140, Length=315, Percent_Identity=33.968253968254, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24646446, Length=318, Percent_Identity=33.6477987421384, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24646448, Length=318, Percent_Identity=33.6477987421384, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24646452, Length=318, Percent_Identity=33.6477987421384, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24646450, Length=318, Percent_Identity=33.6477987421384, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI62472511, Length=318, Percent_Identity=33.6477987421384, Blast_Score=147, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24585516, Length=317, Percent_Identity=29.0220820189274, Blast_Score=135, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: NA

Molecular weight: Translated: 35391; Mature: 35260

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKILVAGKIPEIGLELLKDHNVEMYNKEELISIDELTERVKDKDALLSLLSTKVPKEVI
CCEEEEECCCCHHHHHHHHCCCCCEECHHHHEEHHHHHHHHHHHHHHHHHHHHCCCHHHH
DAAPYLKIVANYGAGYDNIDYTYAGEKGIAVTNTPKVSTEATAELTFALLLAAARRIPEG
CCCHHHHHHHHCCCCCCCCCEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
DTLCRTTGFNGWAPLFFLGREVHGKTIGIIGLGEIGKAVAKRAKAFGMDVLYTGPNRKLE
CCEEEECCCCCCCHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCEEEEECCCCCCC
AESELGATYVTLEELLQTADFITINCAYNPKLHHMIDEEQFKMMKKTAYIVNASRGPIMN
CHHHCCCHHHHHHHHHHHCCEEEEEEEECCHHHHHCCHHHHHHHHHHHEEEECCCCCCCH
EAALAHALKTNEIEGAALDVFEFEPKITEELKELKNVVLAPHVGNATFETRDAMAEMAVR
HHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHH
NILAVLNGEEPVTPVNQKVLVTK
HHHHHHCCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
AKILVAGKIPEIGLELLKDHNVEMYNKEELISIDELTERVKDKDALLSLLSTKVPKEVI
CEEEEECCCCHHHHHHHHCCCCCEECHHHHEEHHHHHHHHHHHHHHHHHHHHCCCHHHH
DAAPYLKIVANYGAGYDNIDYTYAGEKGIAVTNTPKVSTEATAELTFALLLAAARRIPEG
CCCHHHHHHHHCCCCCCCCCEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
DTLCRTTGFNGWAPLFFLGREVHGKTIGIIGLGEIGKAVAKRAKAFGMDVLYTGPNRKLE
CCEEEECCCCCCCHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCEEEEECCCCCCC
AESELGATYVTLEELLQTADFITINCAYNPKLHHMIDEEQFKMMKKTAYIVNASRGPIMN
CHHHCCCHHHHHHHHHHHCCEEEEEEEECCHHHHHCCHHHHHHHHHHHEEEECCCCCCCH
EAALAHALKTNEIEGAALDVFEFEPKITEELKELKNVVLAPHVGNATFETRDAMAEMAVR
HHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHH
NILAVLNGEEPVTPVNQKVLVTK
HHHHHHCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hydroxypyruvate; NADPH; glycolate; NADP; gluconate; 2,5-diketo-D-gluconate; 2-keto-L-gulonate [C]

Specific reaction: hydroxypyruvate + NADPH = glycerate + NADP glycolate + NADP = NADPH + glyoxylate NADP + gluconate = NADPH + 2-Dehydro-D-gluconate 2,5-diketo-D-gluconate + NADPH = 5-ketogluconate + NADP 2-keto-L-gulonate + NADPH = L-idonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]