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The map label for this gene is fruA [H]

Identifier: 218232548

GI number: 218232548

Start: 3726944

End: 3728800

Strand: Reverse

Name: fruA [H]

Synonym: BCB4264_A3816

Alternate gene names: 218232548

Gene position: 3728800-3726944 (Counterclockwise)

Preceding gene: 218231935

Following gene: 218233993

Centisome position: 68.81

GC content: 38.18

Gene sequence:

>1857_bases
ATGAAAATTACAGAACTATTAAAAAGGGATACAGTCATTATGGATTTGACAGCTTCAAATAAAGAAGCTGTCATAGATGA
ATTAGTTGAGAAATTAAACGGGGCAAATCGTTTAAATAGTAAAACGGAATTTAAAGAAGCTATTTTAAAGCGGGAGGCCC
AAAGTACGACTGGAATAGGGGAAGGTATTGCAATACCTCATGCGAAGACAAAGGCTGTTAAACAACCATCGATTTGTTTT
GGTAGAAGTGTAAGCGGTATCAATTATGAGTCGCTTGACGGACAGCCAGCACACTTATTCTTTATGATTGCTGCAAGTGA
AGGAGCGAATAACACTCATTTAGAAACGTTGTCTCGTTTATCGACATTATTAATGGATGAAGGATTTCGCAAGCAACTAT
TAGAAGCAAAGGATGAAAGTGAGCTTCTTCAACTATTTGATGAAAAAGAGAATGAAAAAGAAGAAGAGATTGAAGTCGCA
AAACCAGAAGGAAATGAACCGTACGTACTTGCTGTTACAGCTTGTCCAACTGGAATCGCTCACACATATATGGCAGCGGA
TAGCTTAAAAGCAAAAGCGACAGAGTTAGGAATAGCGATTAAAGTTGAAACAAACGGATCAACAGGTATTAAAAACGGTT
TAACGAAAGAGGATATTGAACGTGCAACAGCTATTATTGTTGCGGCGGATAAACAAGTAGAAATGAATCGTTTTGCTGGT
AAACATGTCATTCAAGTGCCAGTTGCTGATGGGATTCGAAAAACGGAAAATCTGCTTAAGCGAGCTGTCAAACAAGATGC
ACCAATCTTTAAAGGTGTAAAAGAGGACGGGAAATCAGAAAGTATTGAGGGAGAAAAAGGATTAGGAATTTATAAGCATT
TAATGAGCGGTGTAAGTAATATGCTTCCATTCGTTGTTGGTGGCGGGATTTTAATAGCGCTAGCGTTTTCGTTTGGTGGT
ATAAAGGCGGAAGGTCCTTTAGCTGAATTGTTCATGTCAATTGGTGGAGGAAAAACAGGTGCGTTTTTATTCCTTGTACC
AATTTTAGCTGGATTTATTGCAAGTTCTATTGCTGATCGTCCTGGTTTTATGCCTGGTGTTGTAGGTGGATTTTTAGCGG
CGAATGCGAATGCTGGATTTTTAGGTGGGTTAATTGCTGGTTTCTTAGCTGGATATGTTGTTTTAGGGTTGAAAAGATTA
TTTTCAGGACTACCAGTACAATTAGAAGGAATTAAACCTGTATTGTTATATCCAGTCTTTGGATTATTGATTACAGGAGT
TGTAATGCAAAAAGTAGTAATTCCGCCTGTAGTAGCATTAAATGAAATGTTGACAGGGTGGTTAAATGGTTTAAACGGTA
CGAATGCTATATTACTAGGTCTTATTTTAGGTGGTATGATGGCAATTGATATGGGGGGGCCAATTAATAAAGCGGCATTT
ACGTTTGGTATTGCTGCAATAGAAGCACAGAATTTTGGAGTGCACTCAGCAGTTATGGCTGGTGGGATGGTACCGCCACT
CGCGATTGCATTCGCAACTACATTCTTTAAATCAAAGTTTACAGAAGCGGAACGTAAATCTGGTTTAACAAATTATATTA
TGGGAGCATCGTTCATTACAGAAGGTGCGATTCCGTTTGCTGCTGCAGATCCGGTTCGAGTAATCGTAAGTTGTGTTGTT
GGTTCAAGTATTGCAGGTGCGTTATCTATGTTATTCCAAATTACATTACCGGCACCGCATGGTGGATTATTTGTAATAGC
GTTAGTAAATAAACCGTTACTATATATTTTCTCTATATTAATTGGAACAATTGTTTCGGCTGTTATGCTAGGTGTTTGGA
AAAAGAAAGTTCAATAA

Upstream 100 bases:

>100_bases
GGGAGTGCAACAGCATTTTCAGCTGATTTATGTAAGAAGGAAAAAGTAGAAGAATTATTGTCGCAAGTAATTGTAGCTAA
GCGATAGGGGGAAGCAACAT

Downstream 100 bases:

>100_bases
CAAATAAAAAGAGAGCAGTTTTAGTAGCTGCTCTCTTTTTATTACAAAAAAATAGGTTAACAATTGAATATTGATGTTTT
TTGGTAAAGGATGTAACGAA

Product: PTS system, fructose-specific IIABC component

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MKITELLKRDTVIMDLTASNKEAVIDELVEKLNGANRLNSKTEFKEAILKREAQSTTGIGEGIAIPHAKTKAVKQPSICF
GRSVSGINYESLDGQPAHLFFMIAASEGANNTHLETLSRLSTLLMDEGFRKQLLEAKDESELLQLFDEKENEKEEEIEVA
KPEGNEPYVLAVTACPTGIAHTYMAADSLKAKATELGIAIKVETNGSTGIKNGLTKEDIERATAIIVAADKQVEMNRFAG
KHVIQVPVADGIRKTENLLKRAVKQDAPIFKGVKEDGKSESIEGEKGLGIYKHLMSGVSNMLPFVVGGGILIALAFSFGG
IKAEGPLAELFMSIGGGKTGAFLFLVPILAGFIASSIADRPGFMPGVVGGFLAANANAGFLGGLIAGFLAGYVVLGLKRL
FSGLPVQLEGIKPVLLYPVFGLLITGVVMQKVVIPPVVALNEMLTGWLNGLNGTNAILLGLILGGMMAIDMGGPINKAAF
TFGIAAIEAQNFGVHSAVMAGGMVPPLAIAFATTFFKSKFTEAERKSGLTNYIMGASFITEGAIPFAAADPVRVIVSCVV
GSSIAGALSMLFQITLPAPHGGLFVIALVNKPLLYIFSILIGTIVSAVMLGVWKKKVQ

Sequences:

>Translated_618_residues
MKITELLKRDTVIMDLTASNKEAVIDELVEKLNGANRLNSKTEFKEAILKREAQSTTGIGEGIAIPHAKTKAVKQPSICF
GRSVSGINYESLDGQPAHLFFMIAASEGANNTHLETLSRLSTLLMDEGFRKQLLEAKDESELLQLFDEKENEKEEEIEVA
KPEGNEPYVLAVTACPTGIAHTYMAADSLKAKATELGIAIKVETNGSTGIKNGLTKEDIERATAIIVAADKQVEMNRFAG
KHVIQVPVADGIRKTENLLKRAVKQDAPIFKGVKEDGKSESIEGEKGLGIYKHLMSGVSNMLPFVVGGGILIALAFSFGG
IKAEGPLAELFMSIGGGKTGAFLFLVPILAGFIASSIADRPGFMPGVVGGFLAANANAGFLGGLIAGFLAGYVVLGLKRL
FSGLPVQLEGIKPVLLYPVFGLLITGVVMQKVVIPPVVALNEMLTGWLNGLNGTNAILLGLILGGMMAIDMGGPINKAAF
TFGIAAIEAQNFGVHSAVMAGGMVPPLAIAFATTFFKSKFTEAERKSGLTNYIMGASFITEGAIPFAAADPVRVIVSCVV
GSSIAGALSMLFQITLPAPHGGLFVIALVNKPLLYIFSILIGTIVSAVMLGVWKKKVQ
>Mature_618_residues
MKITELLKRDTVIMDLTASNKEAVIDELVEKLNGANRLNSKTEFKEAILKREAQSTTGIGEGIAIPHAKTKAVKQPSICF
GRSVSGINYESLDGQPAHLFFMIAASEGANNTHLETLSRLSTLLMDEGFRKQLLEAKDESELLQLFDEKENEKEEEIEVA
KPEGNEPYVLAVTACPTGIAHTYMAADSLKAKATELGIAIKVETNGSTGIKNGLTKEDIERATAIIVAADKQVEMNRFAG
KHVIQVPVADGIRKTENLLKRAVKQDAPIFKGVKEDGKSESIEGEKGLGIYKHLMSGVSNMLPFVVGGGILIALAFSFGG
IKAEGPLAELFMSIGGGKTGAFLFLVPILAGFIASSIADRPGFMPGVVGGFLAANANAGFLGGLIAGFLAGYVVLGLKRL
FSGLPVQLEGIKPVLLYPVFGLLITGVVMQKVVIPPVVALNEMLTGWLNGLNGTNAILLGLILGGMMAIDMGGPINKAAF
TFGIAAIEAQNFGVHSAVMAGGMVPPLAIAFATTFFKSKFTEAERKSGLTNYIMGASFITEGAIPFAAADPVRVIVSCVV
GSSIAGALSMLFQITLPAPHGGLFVIALVNKPLLYIFSILIGTIVSAVMLGVWKKKVQ

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=458, Percent_Identity=45.1965065502183, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1786951, Length=643, Percent_Identity=33.7480559875583, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI87082348, Length=438, Percent_Identity=34.703196347032, Blast_Score=219, Evalue=6e-58,
Organism=Escherichia coli, GI1790386, Length=313, Percent_Identity=38.9776357827476, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI1788729, Length=381, Percent_Identity=25.7217847769029, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1790387, Length=94, Percent_Identity=41.4893617021277, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788730, Length=94, Percent_Identity=40.4255319148936, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788726, Length=147, Percent_Identity=32.6530612244898, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789597, Length=156, Percent_Identity=28.8461538461538, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 65292; Mature: 65292

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITELLKRDTVIMDLTASNKEAVIDELVEKLNGANRLNSKTEFKEAILKREAQSTTGIG
CCHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCC
EGIAIPHAKTKAVKQPSICFGRSVSGINYESLDGQPAHLFFMIAASEGANNTHLETLSRL
CCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHH
STLLMDEGFRKQLLEAKDESELLQLFDEKENEKEEEIEVAKPEGNEPYVLAVTACPTGIA
HHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHEEECCCCCCCEEEEEECCCCCHH
HTYMAADSLKAKATELGIAIKVETNGSTGIKNGLTKEDIERATAIIVAADKQVEMNRFAG
HHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHEEEEECCCCHHHHHHCC
KHVIQVPVADGIRKTENLLKRAVKQDAPIFKGVKEDGKSESIEGEKGLGIYKHLMSGVSN
CEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
MLPFVVGGGILIALAFSFGGIKAEGPLAELFMSIGGGKTGAFLFLVPILAGFIASSIADR
HHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
PGFMPGVVGGFLAANANAGFLGGLIAGFLAGYVVLGLKRLFSGLPVQLEGIKPVLLYPVF
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHH
GLLITGVVMQKVVIPPVVALNEMLTGWLNGLNGTNAILLGLILGGMMAIDMGGPINKAAF
HHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEECCCCCCHHHH
TFGIAAIEAQNFGVHSAVMAGGMVPPLAIAFATTFFKSKFTEAERKSGLTNYIMGASFIT
HHHHHEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
EGAIPFAAADPVRVIVSCVVGSSIAGALSMLFQITLPAPHGGLFVIALVNKPLLYIFSIL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCHHHHHHHH
IGTIVSAVMLGVWKKKVQ
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKITELLKRDTVIMDLTASNKEAVIDELVEKLNGANRLNSKTEFKEAILKREAQSTTGIG
CCHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCC
EGIAIPHAKTKAVKQPSICFGRSVSGINYESLDGQPAHLFFMIAASEGANNTHLETLSRL
CCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHH
STLLMDEGFRKQLLEAKDESELLQLFDEKENEKEEEIEVAKPEGNEPYVLAVTACPTGIA
HHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHEEECCCCCCCEEEEEECCCCCHH
HTYMAADSLKAKATELGIAIKVETNGSTGIKNGLTKEDIERATAIIVAADKQVEMNRFAG
HHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHEEEEECCCCHHHHHHCC
KHVIQVPVADGIRKTENLLKRAVKQDAPIFKGVKEDGKSESIEGEKGLGIYKHLMSGVSN
CEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
MLPFVVGGGILIALAFSFGGIKAEGPLAELFMSIGGGKTGAFLFLVPILAGFIASSIADR
HHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
PGFMPGVVGGFLAANANAGFLGGLIAGFLAGYVVLGLKRLFSGLPVQLEGIKPVLLYPVF
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHH
GLLITGVVMQKVVIPPVVALNEMLTGWLNGLNGTNAILLGLILGGMMAIDMGGPINKAAF
HHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEECCCCCCHHHH
TFGIAAIEAQNFGVHSAVMAGGMVPPLAIAFATTFFKSKFTEAERKSGLTNYIMGASFIT
HHHHHEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
EGAIPFAAADPVRVIVSCVVGSSIAGALSMLFQITLPAPHGGLFVIALVNKPLLYIFSIL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCHHHHHHHH
IGTIVSAVMLGVWKKKVQ
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]