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The map label for this gene is bmr3 [H]

Identifier: 218232535

GI number: 218232535

Start: 3053115

End: 3054638

Strand: Reverse

Name: bmr3 [H]

Synonym: BCB4264_A3171

Alternate gene names: 218232535

Gene position: 3054638-3053115 (Counterclockwise)

Preceding gene: 218232079

Following gene: 218232981

Centisome position: 56.37

GC content: 34.45

Gene sequence:

>1524_bases
ATGGTTGCAAAAGAGAGCAACTTACGCTTAGTGATAGCTGGGTTAATGCTAGGCATTTTAATGGCTGCAATGGATAATAC
AATTGTTGCCACTTCAATTGGTACAATTGTCTCAGATTTGGGTGGATTGGATAAATTCATCTGGGTTACTTCTGCTTATA
TGGTTGCAGTTATGGCGGGTATGCCTATTTTTGGGAAATTATCTGATATGTATGGTAGAAAGAGGTTTTATATTTTTGGT
TTATTAATCTTCTTAATTGGTTCTGCATTATGTGGTACTGCGCAAAGTATTGTTCAGCTAAGTATTTATCGTGCAATTCA
AGGTATAGGTGGGGGGGCACTGATGCCAATCGCCTTTACCATTGTATTTGATATTTTTCCTATTGAAAAAAGAGGGAAAA
TGACTGGATTATTAGGGGCCGTATTTGGTTCTTCAAGTGTATTAGGACCTTTATTAGGTGCGTATATAACAGAAAATATA
AGTTGGCATTGGATTTTTTACGTCAATATTCCAATTGGTCTAATGTCATTAATTTTTATTTTAAAAGCTTATAAAGAAAA
TAATCCACCTACTAAGCAAAATATTGATTGGTGGGGGGCGATAACACTCGTCATAGCAGTAATTAGCTTAATGTTCGCTT
TAGAGTTGGGAGGAAAAGAATTTAAGTGGGATTCGATACAAATCATTGGGTTATTTGCATCGTTTATTGTATTTTTTGTT
GGCTTCCTATTGGTAGAAAGAAAAGTGAAAGATCCGATAATATCATTTTGGATGTTTAAGCGTAAGGAGTTTGCAATATC
ACAAATTATTACGTTTTTCTATGGAGCAACATTTGTTATTTTAACAGTATTTATTCCTATTTTTGTTCAGGCTGTATATG
GTGGAACAGCAACTAATGCTGGATTAATTTTAACCCCAATGATGTTAGGTTCTGTAGTAGGTAGTGCAATTGGTGGAATA
TTTCAAACGAAAATTAGTTACCGCAACTTGATGTTATTATCTATAATATCCTTTGGGATAGGTATGTATTTGTTAGGAAC
GATGGATCCACAAACAAGCCGTGTACTTTTAACGATATACATGATTATAACGGGGTTTGGAGTTGGATTTTCGTTCTCTT
TGCTTCCCACAGTTTCTATTCATAATATGGAACCGAGATTCCGTGGTTCAGCTACTTCAACAAATTCATTCTTTCGTTCA
CTAGGTATGACAATTGGAGTAACGATTTTTGGCTCCATTCAAAATAGTACCTTTACAGCTAACTTAAAGGATGCTTTTAA
GGGAATAGAACATGGTTCCAGCATGTTAAATAATCTAGGTGATCCGAGTGCAATATTTCGCTCAGATGTAAGGTCCAGTA
TACCTACTTTTATTCTTGAAAAGGTTATAGCTGCTATGTCATCTTCCATTACTACTGTTTTTCTATTAGCTTTAATTCCA
ATTATCGTTTCGTTTATGGCAATATTATTTATGGGGAATGTCAAAGCTGGTACAAAGGATAATAAAGTGAATTTGAAGTT
ATAA

Upstream 100 bases:

>100_bases
TTTTATGATTTATTGAAAAATAGTCTAATTAGACTATAATGAGTATATAGTTTAATTAGACTATATATTTTATTGAGTGT
GAAAAAAGGAGGAAAGAGGT

Downstream 100 bases:

>100_bases
ATTTTATAAAACAAAAAGATTCTTTACTTATTTTTAGCTTATACTATTGGTATAGGTTATTAAGGAGTGGAGAGTTATGA
ATACAAAGCTTTTTATGCTG

Product: Mdr

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MVAKESNLRLVIAGLMLGILMAAMDNTIVATSIGTIVSDLGGLDKFIWVTSAYMVAVMAGMPIFGKLSDMYGRKRFYIFG
LLIFLIGSALCGTAQSIVQLSIYRAIQGIGGGALMPIAFTIVFDIFPIEKRGKMTGLLGAVFGSSSVLGPLLGAYITENI
SWHWIFYVNIPIGLMSLIFILKAYKENNPPTKQNIDWWGAITLVIAVISLMFALELGGKEFKWDSIQIIGLFASFIVFFV
GFLLVERKVKDPIISFWMFKRKEFAISQIITFFYGATFVILTVFIPIFVQAVYGGTATNAGLILTPMMLGSVVGSAIGGI
FQTKISYRNLMLLSIISFGIGMYLLGTMDPQTSRVLLTIYMIITGFGVGFSFSLLPTVSIHNMEPRFRGSATSTNSFFRS
LGMTIGVTIFGSIQNSTFTANLKDAFKGIEHGSSMLNNLGDPSAIFRSDVRSSIPTFILEKVIAAMSSSITTVFLLALIP
IIVSFMAILFMGNVKAGTKDNKVNLKL

Sequences:

>Translated_507_residues
MVAKESNLRLVIAGLMLGILMAAMDNTIVATSIGTIVSDLGGLDKFIWVTSAYMVAVMAGMPIFGKLSDMYGRKRFYIFG
LLIFLIGSALCGTAQSIVQLSIYRAIQGIGGGALMPIAFTIVFDIFPIEKRGKMTGLLGAVFGSSSVLGPLLGAYITENI
SWHWIFYVNIPIGLMSLIFILKAYKENNPPTKQNIDWWGAITLVIAVISLMFALELGGKEFKWDSIQIIGLFASFIVFFV
GFLLVERKVKDPIISFWMFKRKEFAISQIITFFYGATFVILTVFIPIFVQAVYGGTATNAGLILTPMMLGSVVGSAIGGI
FQTKISYRNLMLLSIISFGIGMYLLGTMDPQTSRVLLTIYMIITGFGVGFSFSLLPTVSIHNMEPRFRGSATSTNSFFRS
LGMTIGVTIFGSIQNSTFTANLKDAFKGIEHGSSMLNNLGDPSAIFRSDVRSSIPTFILEKVIAAMSSSITTVFLLALIP
IIVSFMAILFMGNVKAGTKDNKVNLKL
>Mature_507_residues
MVAKESNLRLVIAGLMLGILMAAMDNTIVATSIGTIVSDLGGLDKFIWVTSAYMVAVMAGMPIFGKLSDMYGRKRFYIFG
LLIFLIGSALCGTAQSIVQLSIYRAIQGIGGGALMPIAFTIVFDIFPIEKRGKMTGLLGAVFGSSSVLGPLLGAYITENI
SWHWIFYVNIPIGLMSLIFILKAYKENNPPTKQNIDWWGAITLVIAVISLMFALELGGKEFKWDSIQIIGLFASFIVFFV
GFLLVERKVKDPIISFWMFKRKEFAISQIITFFYGATFVILTVFIPIFVQAVYGGTATNAGLILTPMMLGSVVGSAIGGI
FQTKISYRNLMLLSIISFGIGMYLLGTMDPQTSRVLLTIYMIITGFGVGFSFSLLPTVSIHNMEPRFRGSATSTNSFFRS
LGMTIGVTIFGSIQNSTFTANLKDAFKGIEHGSSMLNNLGDPSAIFRSDVRSSIPTFILEKVIAAMSSSITTVFLLALIP
IIVSFMAILFMGNVKAGTKDNKVNLKL

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=413, Percent_Identity=30.9927360774818, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1788710, Length=447, Percent_Identity=27.7404921700224, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1790195, Length=404, Percent_Identity=24.5049504950495, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87081983, Length=409, Percent_Identity=25.9168704156479, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1788392, Length=398, Percent_Identity=27.3869346733668, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1790146, Length=162, Percent_Identity=29.6296296296296, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788509, Length=144, Percent_Identity=26.3888888888889, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=469, Percent_Identity=23.0277185501066, Blast_Score=120, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6319770, Length=403, Percent_Identity=28.7841191066998, Blast_Score=116, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6323735, Length=345, Percent_Identity=28.695652173913, Blast_Score=113, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6321663, Length=544, Percent_Identity=22.7941176470588, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6325455, Length=467, Percent_Identity=23.7687366167024, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6319783, Length=372, Percent_Identity=21.505376344086, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324954, Length=407, Percent_Identity=23.3415233415233, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55240; Mature: 55240

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MVAKESNLRLVIAGLMLGILMAAMDNTIVATSIGTIVSDLGGLDKFIWVTSAYMVAVMAG
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
MPIFGKLSDMYGRKRFYIFGLLIFLIGSALCGTAQSIVQLSIYRAIQGIGGGALMPIAFT
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
IVFDIFPIEKRGKMTGLLGAVFGSSSVLGPLLGAYITENISWHWIFYVNIPIGLMSLIFI
HHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHH
LKAYKENNPPTKQNIDWWGAITLVIAVISLMFALELGGKEFKWDSIQIIGLFASFIVFFV
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
GFLLVERKVKDPIISFWMFKRKEFAISQIITFFYGATFVILTVFIPIFVQAVYGGTATNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GLILTPMMLGSVVGSAIGGIFQTKISYRNLMLLSIISFGIGMYLLGTMDPQTSRVLLTIY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
MIITGFGVGFSFSLLPTVSIHNMEPRFRGSATSTNSFFRSLGMTIGVTIFGSIQNSTFTA
HHHHHCCCCCEEEHCCEEEEECCCCCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCEEEE
NLKDAFKGIEHGSSMLNNLGDPSAIFRSDVRSSIPTFILEKVIAAMSSSITTVFLLALIP
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIVSFMAILFMGNVKAGTKDNKVNLKL
HHHHHHHHHHHCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]