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The map label for this gene is miaA [H]

Identifier: 218232488

GI number: 218232488

Start: 3724203

End: 3725156

Strand: Reverse

Name: miaA [H]

Synonym: BCB4264_A3813

Alternate gene names: 218232488

Gene position: 3725156-3724203 (Counterclockwise)

Preceding gene: 218233993

Following gene: 218232048

Centisome position: 68.74

GC content: 37.63

Gene sequence:

>954_bases
ATGGGAGAAGTGCAACGTGAAAAAGTTGCTGTCATCATTGGACCAACTGCTGTTGGAAAGACGAAGTTAAGTATCGATCT
TGCCAAAGCGTTGAACGGTGAAATTATTAGTGGTGATTCCATGCAGATCTATCGAACGATGGATATTGGAACTGCAAAGG
TAACAAAAGCAGAGATGGACGGAATTCCACATTATATGATCGATATAAAAAATCCGGAAGATTCATTTTCTGTCGCGGAA
TTTCAAGAACGTGTTCGCAAGCATATTCGAGAAATTACAGAACGTGGTAAATTACCAATTATAGTTGGTGGGACCGGTCT
TTATATACAATCTGTTTTATTCGATTATCAGTTTACAGATGATGCTGGTGATGTGATATACCGAGAGCAAATGGAAAAGT
TAGCTTTAGAACGCGGTGTGGAATATGTACATAAAAAATTGCAAGAAGTAGATCCAGAAAGTGCGGAGCGTATTCATGCA
AATAATGTACGACGTGTCATTCGAGCGTTAGAAATTTTCCACATGACGGGTGAAAAAATGAGTGACCAAATTGAGAAACA
AGAAAAAGAGTTACTCTATGATGTTTCATTAATTGGCTTGACAATGGATCGAGAAATGCTATACGATCGCATTAACTTAC
GAGTGGATTTGATGATGGAACAAGGCCTATTAGAGGAAGTAGAAGGGCTGTATAATAGAGGGATACGAGATTGTCAATCT
ATTCAAGCGATTGGATATAAAGAAATCTATGATTATTTTGAGAATCGAGCCTCTTTAGAAGATGCGGTGTCACAATTAAA
GACGAATTCGCGTCGTTATGCAAAACGTCAATTAACGTGGTTCCGTAATAAAATGGATGTCACGTGGTTTGATGTTACAG
ACGGTGAAAAAACGTCAGAAATTTTACGATACATAGAAGGAAAGTTACAAGTAAAGTCGAATAATAGTAAGTAG

Upstream 100 bases:

>100_bases
CATGTACCGAAATGAAAGAACTTGCAATCATGCCGTTAAAATTGGTAAGATTGGGTATAATCAATAGATGGAGTATATTA
CATAAAGAGAGTTGAATGAT

Downstream 100 bases:

>100_bases
AGAAAAAGAGGAGGATTCGACATGAAGCAATCAATCAATATTCAAGATCAGTTTTTAAATCAACTCCGTAAAGAAAATAC
GTTTGTTACGCTGTACTTAT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MGEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKAEMDGIPHYMIDIKNPEDSFSVAE
FQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDVIYREQMEKLALERGVEYVHKKLQEVDPESAERIHA
NNVRRVIRALEIFHMTGEKMSDQIEKQEKELLYDVSLIGLTMDREMLYDRINLRVDLMMEQGLLEEVEGLYNRGIRDCQS
IQAIGYKEIYDYFENRASLEDAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYIEGKLQVKSNNSK

Sequences:

>Translated_317_residues
MGEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKAEMDGIPHYMIDIKNPEDSFSVAE
FQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDVIYREQMEKLALERGVEYVHKKLQEVDPESAERIHA
NNVRRVIRALEIFHMTGEKMSDQIEKQEKELLYDVSLIGLTMDREMLYDRINLRVDLMMEQGLLEEVEGLYNRGIRDCQS
IQAIGYKEIYDYFENRASLEDAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYIEGKLQVKSNNSK
>Mature_316_residues
GEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKAEMDGIPHYMIDIKNPEDSFSVAEF
QERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDVIYREQMEKLALERGVEYVHKKLQEVDPESAERIHAN
NVRRVIRALEIFHMTGEKMSDQIEKQEKELLYDVSLIGLTMDREMLYDRINLRVDLMMEQGLLEEVEGLYNRGIRDCQSI
QAIGYKEIYDYFENRASLEDAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYIEGKLQVKSNNSK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=310, Percent_Identity=32.258064516129, Blast_Score=155, Evalue=4e-38,
Organism=Escherichia coli, GI1790613, Length=302, Percent_Identity=37.4172185430464, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI25144712, Length=307, Percent_Identity=32.8990228013029, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324848, Length=307, Percent_Identity=34.8534201954397, Blast_Score=181, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24649830, Length=341, Percent_Identity=30.791788856305, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 36637; Mature: 36506

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKAEMD
CCCCCCCCEEEEECCCCCCCEEHHEEHHHHCCCCEECCCCEEEEEEECCCCHHEEHHHHC
GIPHYMIDIKNPEDSFSVAEFQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCEECC
DAGDVIYREQMEKLALERGVEYVHKKLQEVDPESAERIHANNVRRVIRALEIFHMTGEKM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHH
SDQIEKQEKELLYDVSLIGLTMDREMLYDRINLRVDLMMEQGLLEEVEGLYNRGIRDCQS
HHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHCCHHHHHH
IQAIGYKEIYDYFENRASLEDAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSE
HHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
ILRYIEGKLQVKSNNSK
HHHHHCCEEEEECCCCC
>Mature Secondary Structure 
GEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKAEMD
CCCCCCCEEEEECCCCCCCEEHHEEHHHHCCCCEECCCCEEEEEEECCCCHHEEHHHHC
GIPHYMIDIKNPEDSFSVAEFQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCEECC
DAGDVIYREQMEKLALERGVEYVHKKLQEVDPESAERIHANNVRRVIRALEIFHMTGEKM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHH
SDQIEKQEKELLYDVSLIGLTMDREMLYDRINLRVDLMMEQGLLEEVEGLYNRGIRDCQS
HHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHCCHHHHHH
IQAIGYKEIYDYFENRASLEDAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSE
HHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
ILRYIEGKLQVKSNNSK
HHHHHCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA