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The map label for this gene is tenA [H]

Identifier: 218232420

GI number: 218232420

Start: 761504

End: 762199

Strand: Direct

Name: tenA [H]

Synonym: BCB4264_A0782

Alternate gene names: 218232420

Gene position: 761504-762199 (Clockwise)

Preceding gene: 218235594

Following gene: 218233062

Centisome position: 14.05

GC content: 34.34

Gene sequence:

>696_bases
ATGAAATTTTGCAATAGATTATTAGAAACTGTGCAACCGGTATGGGAGATGAGTCATAATCATCCGTTTGTAGTGGGTAT
GGGAGATGGCACGTTAGAAAAAGATAAATTCCAGTATTATATTATTCAAGATTATTTATATCTGCTAGATTATGCAAAGT
TATATGCGATTGGTGTTGTAAAAGCAACGAATCCACAAGTAATGGGAAAGTTCGCTGAACAAATTGATGGAATATTAAAT
GGCGAAATGACAATCCATAAACAATATGCAAAAAGACTTGGAATTTCTATAGAGGAGATAGAGTCTGCGAAACCATCTGC
TAAAAATTTAGCTTATACAAATTACATGATGTCTGTATCTCAAAATGGCACACTTGCGGAATTAATAGCAGCGCTTCTTC
CATGTATGTGGAGCTATTGGGAAATTGGAAAGCGTTTAAATGATATTCCTAGAGCAAGAGATCATGAGTTTTTTGGTGAG
TGGATTCAAGGATATAGTTCGGAAGAATACGGTAATCTTTGTATTTGGTTAATAGATTTATTAAATGAAATGGCAGTTGG
AAAGTCTGAGAAAGAACTAGAAAGATTAGAGGAGATTTTCTTAAATTCCAGTCGATTTGAATATTTATTCTGGGATATGT
CCTATCGTAAGGAGATGTGGGGATTTGAGGAGCAAGAACATACTACAGTTTCATAA

Upstream 100 bases:

>100_bases
TGGAACATCGAATAAATGTATAACTTTATTTGCTGTAACCACTTCTTATATATAGAAGTGGTTTTTTATTTAGATACATA
TATTGAAAGGGGATAGAGAA

Downstream 100 bases:

>100_bases
TGTTTCTTTTCATTACGATGAGAAACCAATCACCCATGAATTAAATGCTTCTATACATGAGAAAGAGTTTGTCAGTATTA
TCGGACCGAGTGGATGTGGG

Product: putative transcriptional activator TenA

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKFCNRLLETVQPVWEMSHNHPFVVGMGDGTLEKDKFQYYIIQDYLYLLDYAKLYAIGVVKATNPQVMGKFAEQIDGILN
GEMTIHKQYAKRLGISIEEIESAKPSAKNLAYTNYMMSVSQNGTLAELIAALLPCMWSYWEIGKRLNDIPRARDHEFFGE
WIQGYSSEEYGNLCIWLIDLLNEMAVGKSEKELERLEEIFLNSSRFEYLFWDMSYRKEMWGFEEQEHTTVS

Sequences:

>Translated_231_residues
MKFCNRLLETVQPVWEMSHNHPFVVGMGDGTLEKDKFQYYIIQDYLYLLDYAKLYAIGVVKATNPQVMGKFAEQIDGILN
GEMTIHKQYAKRLGISIEEIESAKPSAKNLAYTNYMMSVSQNGTLAELIAALLPCMWSYWEIGKRLNDIPRARDHEFFGE
WIQGYSSEEYGNLCIWLIDLLNEMAVGKSEKELERLEEIFLNSSRFEYLFWDMSYRKEMWGFEEQEHTTVS
>Mature_231_residues
MKFCNRLLETVQPVWEMSHNHPFVVGMGDGTLEKDKFQYYIIQDYLYLLDYAKLYAIGVVKATNPQVMGKFAEQIDGILN
GEMTIHKQYAKRLGISIEEIESAKPSAKNLAYTNYMMSVSQNGTLAELIAALLPCMWSYWEIGKRLNDIPRARDHEFFGE
WIQGYSSEEYGNLCIWLIDLLNEMAVGKSEKELERLEEIFLNSSRFEYLFWDMSYRKEMWGFEEQEHTTVS

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=210, Percent_Identity=31.4285714285714, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324997, Length=205, Percent_Identity=29.7560975609756, Blast_Score=89, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6325378, Length=203, Percent_Identity=28.0788177339901, Blast_Score=85, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 27027; Mature: 27027

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFCNRLLETVQPVWEMSHNHPFVVGMGDGTLEKDKFQYYIIQDYLYLLDYAKLYAIGVV
CHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHE
KATNPQVMGKFAEQIDGILNGEMTIHKQYAKRLGISIEEIESAKPSAKNLAYTNYMMSVS
ECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHCC
QNGTLAELIAALLPCMWSYWEIGKRLNDIPRARDHEFFGEWIQGYSSEEYGNLCIWLIDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHH
LNEMAVGKSEKELERLEEIFLNSSRFEYLFWDMSYRKEMWGFEEQEHTTVS
HHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKFCNRLLETVQPVWEMSHNHPFVVGMGDGTLEKDKFQYYIIQDYLYLLDYAKLYAIGVV
CHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHE
KATNPQVMGKFAEQIDGILNGEMTIHKQYAKRLGISIEEIESAKPSAKNLAYTNYMMSVS
ECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHCC
QNGTLAELIAALLPCMWSYWEIGKRLNDIPRARDHEFFGEWIQGYSSEEYGNLCIWLIDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHH
LNEMAVGKSEKELERLEEIFLNSSRFEYLFWDMSYRKEMWGFEEQEHTTVS
HHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]