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The map label for this gene is gyrB [H]

Identifier: 218232402

GI number: 218232402

Start: 4590

End: 6512

Strand: Direct

Name: gyrB [H]

Synonym: BCB4264_A0005

Alternate gene names: 218232402

Gene position: 4590-6512 (Clockwise)

Preceding gene: 218232841

Following gene: 218231332

Centisome position: 0.08

GC content: 37.7

Gene sequence:

>1923_bases
ATGGAACAAAAGCAAATGCAAGAAAATGCATATGATGAAAGTCAGATACAGGTGCTTGAAGGACTAGAGGCAGTTCGGAA
ACGCCCTGGTATGTATATTGGATCTACAAGTGGAAAAGGGCTTCACCATCTTGTTTGGGAAATCGTAGATAATAGTATTG
ATGAAGCACTTGCAGGTTATTGTGATGAAATTAATGTTAGTATCGAAGAAGATAATAGTATTCGTGTAACGGATAATGGG
CGTGGTATTCCGGTTGGTATACAAGAAAAAATGGGACGTCCAGCTGTAGAAGTTATTATGACTGTTCTCCACGCCGGTGG
TAAATTTGGCGGTGGCGGTTATAAAGTTTCTGGTGGTTTACATGGTGTTGGGGCATCGGTTGTAAATGCTCTATCAACAG
AATTGGAAGTATTTGTACATCGTGAAGGTAAAATCCATTACCAAAAATACGAAAGAGGTATTCCGGTTGCGGATTTAAAA
GTCATTGGTGACACAGATCAAACAGGAACAATAACTCGATTTAAACCAGATCCGGAAATTTTCCAAGAAACAACAGTATA
CGATTTTGATACGCTAGCAACTCGTATGCGTGAATTAGCGTTTTTAAATCGTAATATTAAATTAACAATTGAAGATAAAC
GTGAACATAAGCAAAAGAAAGAATTCCATTACGAAGGTGGAATTAAATCATACGTTGAGCATTTAAATCGCTCAAAACAA
CCGATTCATGAAGAGCCTGTGTACGTAGAAGGTTCAAAAGATGGTATTCAGGTTGAGGTTTCTCTTCAATATAACGAAGG
ATACACAAATAATATTTACTCATTTACGAATAACATCCATACGTATGAAGGTGGTACACATGAGGTAGGTTTTAAAACAG
CTTTAACTCGTGTAATCAACGACTATGGTCGTAAAAATAGCATTTTAAAAGATGCGGACAGTAATTTAACTGGTGAGGAT
GTTCGTGAAGGTTTAACAGCAATTGTATCAATCAAGCATCCAAATCCACAATTTGAAGGACAAACGAAGACAAAACTTGG
GAATAGTGAAGCGAGAACAATTACAGAGTCTGTATTCTCAGAGGCATTTGAAAAGTTCTTACTAGAAAATCCTAATGTAG
CGCGAAAAATTGTAGAAAAAGGTACGATGGCTGCACGTGCACGTGTAGCTGCGAAAAAAGCGCGTGAATTGACACGTCGA
AAGAGTGCGTTAGAAGTTTCAAGTTTACCTGGTAAATTAGCTGATTGCTCTTCGAAAGATCCAGCAATTAGTGAAATTTA
CATCGTAGAGGGTGACTCTGCGGGTGGATCTGCAAAACAAGGACGCGATCGTCATTTCCAAGCAATTTTACCGCTGAAGG
GTAAAATTATTAATGTGGAAAAGGCGCGCTTAGATAAGATTTTATCAAATGATGAAGTTCGTACAATTATTACGGCAATC
GGTACAAATATTGGTGGAGACTTCGATATTGAAAAAGCACGCTATCATAAAGTTATTATTATGACCGATGCCGATGTTGA
TGGTGCACATATTCGTACCCTATTATTAACGTTCTTCTATCGTTATATGCGTCAAATAATTGAGTGTGGTTATATCTATA
TTGCACAGCCGCCATTGTTTAAAATACAACAAGGTAAAAAAATTCAATATGCTTACAACGATAAGGAACTTGAAAAAATC
TTAGCTGAGCTACCAGCCCAGCCGAAACCAGGAATTCAACGTTATAAAGGTCTTGGGGAGATGAATCCAACTCAGCTATG
GGAGACGACAATGGATCCAGAAGTACGTTCGTTACTTCAAGTTTCTCTTCAAGATGCAATAGAAGCAGACGAAACTTTTG
AAATTTTAATGGGTGATAAAGTAGAACCGCGTCGTAACTTTATCCAAGAGAATGCAAAATACGTGAAGAACCTTGATATT
TAA

Upstream 100 bases:

>100_bases
AAGAAGCGAAAACAATTCATGTAACGAACGGCACTGTAGATTGTGAAATAGATAGGGAATAACCCCTGTTTAAATGGAAA
AGTAGGTGATCTTTGTGTCA

Downstream 100 bases:

>100_bases
ATGAGTAATGACAGGAATCTAAGTATTCCTGTCTTCTACATATAAATCAATGTATGTGTAACGGAAATGTAAGAGGAGGT
GCTCGTTGATGTCAGACAAT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 640; Mature: 640

Protein sequence:

>640_residues
MEQKQMQENAYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGYCDEINVSIEEDNSIRVTDNG
RGIPVGIQEKMGRPAVEVIMTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTELEVFVHREGKIHYQKYERGIPVADLK
VIGDTDQTGTITRFKPDPEIFQETTVYDFDTLATRMRELAFLNRNIKLTIEDKREHKQKKEFHYEGGIKSYVEHLNRSKQ
PIHEEPVYVEGSKDGIQVEVSLQYNEGYTNNIYSFTNNIHTYEGGTHEVGFKTALTRVINDYGRKNSILKDADSNLTGED
VREGLTAIVSIKHPNPQFEGQTKTKLGNSEARTITESVFSEAFEKFLLENPNVARKIVEKGTMAARARVAAKKARELTRR
KSALEVSSLPGKLADCSSKDPAISEIYIVEGDSAGGSAKQGRDRHFQAILPLKGKIINVEKARLDKILSNDEVRTIITAI
GTNIGGDFDIEKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRQIIECGYIYIAQPPLFKIQQGKKIQYAYNDKELEKI
LAELPAQPKPGIQRYKGLGEMNPTQLWETTMDPEVRSLLQVSLQDAIEADETFEILMGDKVEPRRNFIQENAKYVKNLDI

Sequences:

>Translated_640_residues
MEQKQMQENAYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGYCDEINVSIEEDNSIRVTDNG
RGIPVGIQEKMGRPAVEVIMTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTELEVFVHREGKIHYQKYERGIPVADLK
VIGDTDQTGTITRFKPDPEIFQETTVYDFDTLATRMRELAFLNRNIKLTIEDKREHKQKKEFHYEGGIKSYVEHLNRSKQ
PIHEEPVYVEGSKDGIQVEVSLQYNEGYTNNIYSFTNNIHTYEGGTHEVGFKTALTRVINDYGRKNSILKDADSNLTGED
VREGLTAIVSIKHPNPQFEGQTKTKLGNSEARTITESVFSEAFEKFLLENPNVARKIVEKGTMAARARVAAKKARELTRR
KSALEVSSLPGKLADCSSKDPAISEIYIVEGDSAGGSAKQGRDRHFQAILPLKGKIINVEKARLDKILSNDEVRTIITAI
GTNIGGDFDIEKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRQIIECGYIYIAQPPLFKIQQGKKIQYAYNDKELEKI
LAELPAQPKPGIQRYKGLGEMNPTQLWETTMDPEVRSLLQVSLQDAIEADETFEILMGDKVEPRRNFIQENAKYVKNLDI
>Mature_640_residues
MEQKQMQENAYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGYCDEINVSIEEDNSIRVTDNG
RGIPVGIQEKMGRPAVEVIMTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTELEVFVHREGKIHYQKYERGIPVADLK
VIGDTDQTGTITRFKPDPEIFQETTVYDFDTLATRMRELAFLNRNIKLTIEDKREHKQKKEFHYEGGIKSYVEHLNRSKQ
PIHEEPVYVEGSKDGIQVEVSLQYNEGYTNNIYSFTNNIHTYEGGTHEVGFKTALTRVINDYGRKNSILKDADSNLTGED
VREGLTAIVSIKHPNPQFEGQTKTKLGNSEARTITESVFSEAFEKFLLENPNVARKIVEKGTMAARARVAAKKARELTRR
KSALEVSSLPGKLADCSSKDPAISEIYIVEGDSAGGSAKQGRDRHFQAILPLKGKIINVEKARLDKILSNDEVRTIITAI
GTNIGGDFDIEKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRQIIECGYIYIAQPPLFKIQQGKKIQYAYNDKELEKI
LAELPAQPKPGIQRYKGLGEMNPTQLWETTMDPEVRSLLQVSLQDAIEADETFEILMGDKVEPRRNFIQENAKYVKNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=609, Percent_Identity=26.9293924466338, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI19913408, Length=627, Percent_Identity=26.3157894736842, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI48994957, Length=553, Percent_Identity=59.1320072332731, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=627, Percent_Identity=39.5534290271132, Blast_Score=441, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17535065, Length=657, Percent_Identity=24.6575342465753, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=541, Percent_Identity=25.5083179297597, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=227, Percent_Identity=26.431718061674, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=616, Percent_Identity=26.2987012987013, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17136538, Length=620, Percent_Identity=25, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71833; Mature: 71833

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQKQMQENAYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGY
CCHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
CDEINVSIEEDNSIRVTDNGRGIPVGIQEKMGRPAVEVIMTVLHAGGKFGGGGYKVSGGL
CCCCEEEEECCCEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGASVVNALSTELEVFVHREGKIHYQKYERGIPVADLKVIGDTDQTGTITRFKPDPEI
CHHHHHHHHHHHHHEEEEEECCCCEEHHHHHCCCCEEEEEEECCCCCCCCEEEECCCHHH
FQETTVYDFDTLATRMRELAFLNRNIKLTIEDKREHKQKKEFHYEGGIKSYVEHLNRSKQ
HHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHCCCCC
PIHEEPVYVEGSKDGIQVEVSLQYNEGYTNNIYSFTNNIHTYEGGTHEVGFKTALTRVIN
CCCCCCEEEECCCCCEEEEEEEEECCCCCCCHHHHCCCEEECCCCCCCCHHHHHHHHHHH
DYGRKNSILKDADSNLTGEDVREGLTAIVSIKHPNPQFEGQTKTKLGNSEARTITESVFS
HHCCCCCCHHCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHHH
EAFEKFLLENPNVARKIVEKGTMAARARVAAKKARELTRRKSALEVSSLPGKLADCSSKD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PAISEIYIVEGDSAGGSAKQGRDRHFQAILPLKGKIINVEKARLDKILSNDEVRTIITAI
CCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHCCCHHHHHHHHH
GTNIGGDFDIEKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRQIIECGYIYIAQPPLF
CCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCE
KIQQGKKIQYAYNDKELEKILAELPAQPKPGIQRYKGLGEMNPTQLWETTMDPEVRSLLQ
EECCCCEEEEEECHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHCCCCHHHHHHHH
VSLQDAIEADETFEILMGDKVEPRRNFIQENAKYVKNLDI
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEQKQMQENAYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSIDEALAGY
CCHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
CDEINVSIEEDNSIRVTDNGRGIPVGIQEKMGRPAVEVIMTVLHAGGKFGGGGYKVSGGL
CCCCEEEEECCCEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGASVVNALSTELEVFVHREGKIHYQKYERGIPVADLKVIGDTDQTGTITRFKPDPEI
CHHHHHHHHHHHHHEEEEEECCCCEEHHHHHCCCCEEEEEEECCCCCCCCEEEECCCHHH
FQETTVYDFDTLATRMRELAFLNRNIKLTIEDKREHKQKKEFHYEGGIKSYVEHLNRSKQ
HHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHCCCCC
PIHEEPVYVEGSKDGIQVEVSLQYNEGYTNNIYSFTNNIHTYEGGTHEVGFKTALTRVIN
CCCCCCEEEECCCCCEEEEEEEEECCCCCCCHHHHCCCEEECCCCCCCCHHHHHHHHHHH
DYGRKNSILKDADSNLTGEDVREGLTAIVSIKHPNPQFEGQTKTKLGNSEARTITESVFS
HHCCCCCCHHCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHHH
EAFEKFLLENPNVARKIVEKGTMAARARVAAKKARELTRRKSALEVSSLPGKLADCSSKD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PAISEIYIVEGDSAGGSAKQGRDRHFQAILPLKGKIINVEKARLDKILSNDEVRTIITAI
CCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHCCCHHHHHHHHH
GTNIGGDFDIEKARYHKVIIMTDADVDGAHIRTLLLTFFYRYMRQIIECGYIYIAQPPLF
CCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCE
KIQQGKKIQYAYNDKELEKILAELPAQPKPGIQRYKGLGEMNPTQLWETTMDPEVRSLLQ
EECCCCEEEEEECHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHCCCCHHHHHHHH
VSLQDAIEADETFEILMGDKVEPRRNFIQENAKYVKNLDI
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629; 10103241 [H]